apobec3g  (Bioss)

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Correlation of APOBEC3G Polymorphism with Human Papillomavirus (HPV) Persistent Infection and Progression of Cervical Lesions

doi: 10.12659/MSM.916142

Figure Lengend Snippet: Expression level of APOBEC3G mRNA in cervical cancer, CIN, and non-cervical lesions. CIN – cervical intraepithelial neoplasia; ** p<0.05.

Article Snippet: The primary antibodies used in this study were as follows: APOBEC3G (Bioss, MA, USA), HPV E6 (Abcam, Cambridge, MA), p53 (Abcam, Cambridge, MA), with GADPH (Abcam, Cambridge, MA) and was considered as the internal reference.

Techniques: Expressing

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Correlation of APOBEC3G Polymorphism with Human Papillomavirus (HPV) Persistent Infection and Progression of Cervical Lesions

doi: 10.12659/MSM.916142

Figure Lengend Snippet: Expression level of APOBEC3G protein in cervical cancer, CIN, and non-cervical lesions. CIN – cervical intraepithelial neoplasia; ** p<0.05.

Article Snippet: The primary antibodies used in this study were as follows: APOBEC3G (Bioss, MA, USA), HPV E6 (Abcam, Cambridge, MA), p53 (Abcam, Cambridge, MA), with GADPH (Abcam, Cambridge, MA) and was considered as the internal reference.

Techniques: Expressing

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Correlation of APOBEC3G Polymorphism with Human Papillomavirus (HPV) Persistent Infection and Progression of Cervical Lesions

doi: 10.12659/MSM.916142

Figure Lengend Snippet: APOBEC3G Exon3 genotype in Uyghur people, Han population, and European and American people.

Article Snippet: The primary antibodies used in this study were as follows: APOBEC3G (Bioss, MA, USA), HPV E6 (Abcam, Cambridge, MA), p53 (Abcam, Cambridge, MA), with GADPH (Abcam, Cambridge, MA) and was considered as the internal reference.

Techniques:

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Correlation of APOBEC3G Polymorphism with Human Papillomavirus (HPV) Persistent Infection and Progression of Cervical Lesions

doi: 10.12659/MSM.916142

Figure Lengend Snippet: Distribution pattern of APOBEC3G gene rs5757465 locus genotype in Uygur cervical lesions.

Article Snippet: The primary antibodies used in this study were as follows: APOBEC3G (Bioss, MA, USA), HPV E6 (Abcam, Cambridge, MA), p53 (Abcam, Cambridge, MA), with GADPH (Abcam, Cambridge, MA) and was considered as the internal reference.

Techniques:

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Correlation of APOBEC3G Polymorphism with Human Papillomavirus (HPV) Persistent Infection and Progression of Cervical Lesions

doi: 10.12659/MSM.916142

Figure Lengend Snippet: Expression levels of HPV E6 and p53 mRNA and protein in SIHA cells transfected with APOBEC3G gene. ( A ) Fluorescent protein in SIHA cells before and after transfection. ( a ) Fluorescent protein in SIHA cells before transfected with APOBEC3G gene; ( b ) Fluorescent protein in SIHA cells after transfected with APOBEC3G gene. ( B ) Expression levels of HPV E6 and p53 protein in SIHA cells transfected with APOBEC3G gene. ( C ) Expression levels of p53 mRNA in SIHA cells transfected with APOBEC3G gene. ( D ) Expression levels of HPV E6 mRNA in SIHA cells transfected with APOBEC3G gene. ** p<0.05.

Article Snippet: The primary antibodies used in this study were as follows: APOBEC3G (Bioss, MA, USA), HPV E6 (Abcam, Cambridge, MA), p53 (Abcam, Cambridge, MA), with GADPH (Abcam, Cambridge, MA) and was considered as the internal reference.

Techniques: Expressing, Transfection

Journal: Oncology Letters

Article Title: Association between APOBEC3s and HPV16 E2 gene hypermutation in Uygur females with cervical cancer

doi: 10.3892/ol.2020.11697

Figure Lengend Snippet: PCR primer sequences.

Article Snippet: After blocking with 5% BSA at room temperature for 1 h, the membrane was incubated with anti-APOBEC3G (1:500; bs-15407R; BIOSS), anti-APOBEC3F (1:500; ab227962; Abcam), anti-APOBEC3C (1:500; bs-12495R; BIOSS) and anti-GAPDH (1:5,000; bs-50549R; BIOSS) primary antibodies at 4°C for 16 h. Subsequently, the membranes were incubated with the secondary antibody of goat anti-rabbit HRP conjugated IgG (1:10,000; ZB-2301; OriGene Technologies, Inc.) at 37°C for 1 h in the dark.

Techniques:

Journal: Oncology Letters

Article Title: Association between APOBEC3s and HPV16 E2 gene hypermutation in Uygur females with cervical cancer

doi: 10.3892/ol.2020.11697

Figure Lengend Snippet: Expression levels of APBOEC3s mRNA in cervical lesion samples of the cervicitis, CIN I–III and cervical cancer groups. The relative mRNA expression levels of (A) A3C, (B) A3F and (C) A3G in the cervical lesions of different grades were detected using reverse transcription-quantitative PCR. *P<0.05, **P<0.01. APBOEC3s, apolipoprotein B mRNA-editing enzyme catalytic polypeptides; CIN I–III, cervical intraepithelial neoplasia I, II and III.

Article Snippet: After blocking with 5% BSA at room temperature for 1 h, the membrane was incubated with anti-APOBEC3G (1:500; bs-15407R; BIOSS), anti-APOBEC3F (1:500; ab227962; Abcam), anti-APOBEC3C (1:500; bs-12495R; BIOSS) and anti-GAPDH (1:5,000; bs-50549R; BIOSS) primary antibodies at 4°C for 16 h. Subsequently, the membranes were incubated with the secondary antibody of goat anti-rabbit HRP conjugated IgG (1:10,000; ZB-2301; OriGene Technologies, Inc.) at 37°C for 1 h in the dark.

Techniques: Expressing, Real-time Polymerase Chain Reaction

Journal: Oncology Letters

Article Title: Association between APOBEC3s and HPV16 E2 gene hypermutation in Uygur females with cervical cancer

doi: 10.3892/ol.2020.11697

Figure Lengend Snippet: Protein expression levels of APBOEC3 in cervical cancer of different grades. (A) Protein expression levels of A3C, A3F and A3G in cervical lesion of different grades detected using western blot analysis. Relative protein expression levels of (B) A3C, (C) A3F and (D) A3G. **P<0.01. APBOEC3s, Apolipoprotein B mRNA-editing enzyme catalytic polypeptides; CIN I–III, cervical intraepithelial neoplasia I, II and III.

Article Snippet: After blocking with 5% BSA at room temperature for 1 h, the membrane was incubated with anti-APOBEC3G (1:500; bs-15407R; BIOSS), anti-APOBEC3F (1:500; ab227962; Abcam), anti-APOBEC3C (1:500; bs-12495R; BIOSS) and anti-GAPDH (1:5,000; bs-50549R; BIOSS) primary antibodies at 4°C for 16 h. Subsequently, the membranes were incubated with the secondary antibody of goat anti-rabbit HRP conjugated IgG (1:10,000; ZB-2301; OriGene Technologies, Inc.) at 37°C for 1 h in the dark.

Techniques: Expressing, Western Blot

Journal: Oncology Letters

Article Title: Association between APOBEC3s and HPV16 E2 gene hypermutation in Uygur females with cervical cancer

doi: 10.3892/ol.2020.11697

Figure Lengend Snippet: HPV16 E2 gene expression and hypermutation in SiHa cells transfected with APOBEC3s lentiviral vectors. (A) GFP-labeled A3C, A3F and A3G lentiviruses were transfected into SiHa cells and the fluorescence intensity was observed using an inverted fluorescence microscope to determine the transfection efficiency. Magnification, ×200. The mRNA and protein expression levels of HPV16 E2 in A3 lentivirus-transfected SiHa cells were confirmed using reverse transcription-quantitative PCR and western blotting. (B) The HPV16 E2 genome hypermutation following lentiviral transfection was detected using the differential DNA Denaturation PCR. **P<0.01 vs. blank control or scrambled siRNA control. HPV, human papilloma virus; APBOEC3s, Apolipoprotein B mRNA-editing enzyme catalytic polypeptides; siRNA, small interfering RNA; NC, negative control; A3C, APOBEC3C; A3F, APOBEC3F; A3G, APOBEC3G; GFP, green fluorescent protein; C, cytosine; G, guanine; A, adenine; T, thymine.

Article Snippet: After blocking with 5% BSA at room temperature for 1 h, the membrane was incubated with anti-APOBEC3G (1:500; bs-15407R; BIOSS), anti-APOBEC3F (1:500; ab227962; Abcam), anti-APOBEC3C (1:500; bs-12495R; BIOSS) and anti-GAPDH (1:5,000; bs-50549R; BIOSS) primary antibodies at 4°C for 16 h. Subsequently, the membranes were incubated with the secondary antibody of goat anti-rabbit HRP conjugated IgG (1:10,000; ZB-2301; OriGene Technologies, Inc.) at 37°C for 1 h in the dark.

Techniques: Expressing, Transfection, Labeling, Fluorescence, Microscopy, Real-time Polymerase Chain Reaction, Western Blot, Small Interfering RNA, Negative Control