Journal: Endocrinology
Article Title: Leukemia Inhibitory Factor Is Necessary for Ovulation in Female Rhesus Macaques
doi: 10.1210/en.2016-1283
Figure Lengend Snippet: Antibody Table
Article Snippet: Any white balance adjustment was made uniformly across all images. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Protein Target Antigen Sequence Antibody Name Manufacturer and Catalog Number Species Raised in; Clonality Dilution Used LIFR KLH conjugated from the middle of human LIFR LIFR/CD118 Bioss, bs-1458R Rabbit; polyclonal 1:200 pY705-STAT3 Phosphopeptide containing human STAT3 Y705 site Human phosho-STAT3 (Y705) R&D Systems, AF4607 Rabbit; polyclonal 1:50 IL6ST Phosphorylation site of serine 782 CD130 (gp130) Abcam, ab59389 Rabbit; polyclonal 1:100 JAK1 Phosphorylation site of tyrosine 1022 JAK1 Abcam, ab47435 Rabbit; polyclonal 1:100 STAT3 Residues surrounding serine 727 of human STAT3 STAT3 Abcam, ab32500 Rabbit; monoclonal 1:100 pS727-STAT3 Human STAT3 700 to the C terminus STAT3 (phospho-S727) Abcam, ab32143 Rabbit; monoclonal 1:650 Isotype control Proprietary isotype control Rabbit IgG Abcam, ab172730 Rabbit; monoclonal 1:50, 100, 200, and 650 Open in a separate window Antibody Table Laparoscopic and histological evaluation of follicle rupture The presence or absence of an ovulatory stigmata was determined by laparoscopy at 72 hours after intrafollicular injection of vehicle (n = 3) or sLIFR (n = 4) and hCG ( 16 ).
Techniques: Sequencing, Control