. RT-qPCR was performed to determine mRNA levels of Ctnnb1 (A) , Cdh1 (B) , Cldn1 (C) , Cldn7 (D) , Cldn13 (E) , Cldn15 (F) , Ocln (G) , Tjp1 (H) , Cldn8 (I) , Muc2 (J) , Muc3 (K) , and Muc4 (L) . n=4 or 5. * p <0.05 vs wild type control. ** p <0.05 vs wild type DSS. " width="100%" height="100%">
Journal: Frontiers in Immunology
Article Title: Matrix metalloproteinase 7 contributes to intestinal barrier dysfunction by degrading tight junction protein Claudin-7
doi: 10.3389/fimmu.2022.1020902
Figure Lengend Snippet: Differential mRNA expression of junction proteins in the colons of wild type and Mmp7 -/- mice treated with 3% DSS. Colitis was induced with 3% DSS in drinking water in both C57BL/6 and Mmp7 -/- mice for 7 days as shown in Figure 4 . RT-qPCR was performed to determine mRNA levels of Ctnnb1 (A) , Cdh1 (B) , Cldn1 (C) , Cldn7 (D) , Cldn13 (E) , Cldn15 (F) , Ocln (G) , Tjp1 (H) , Cldn8 (I) , Muc2 (J) , Muc3 (K) , and Muc4 (L) . n=4 or 5. * p <0.05 vs wild type control. ** p <0.05 vs wild type DSS.
Article Snippet: Primary antibodies were as follows: anti-Claudin-1 rabbit polyclonal (Cat. # A2196, 1:1000) (ABclonal, Woburn, MA), anti-Claudin-7 rabbit polyclonal (Cat. # bs-8482R, 1:1000) (Bioss Antibodies, Woburn, MA), anti-Claudin-8 rabbit polyclonal (Cat. # PIPA124415, 1:1000) (Fisher Scientific), anti-Claudin-15 rabbit polyclonal (Cat. # bs-13753R, 1:1000) (Bioss Antibodies), anti-β-actin mouse monoclonal (Cat. # MA5-15452, 1:3000) (Thermo Fisher), and anti-GADPH rabbit polyclonal (Cat. # 5174S, 1:1000) (Cell Signaling, Danvers, MA).
Techniques: Expressing, Quantitative RT-PCR