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gngt1 primary antibody (Bioss)

Name: gngt1 primary antibody
Brand: Bioss
Rating: 94 (2 reviews)

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Bioss gngt1 primary antibody
Gngt1 Primary Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gngt1 primary antibody/product/Bioss
Average 94 stars, based on 2 article reviews

gngt1 primary antibody - by Bioz Stars, 2026-02

94/100 stars

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GNGT1 expression levels and clinical pathological features in LUAD. (A) Expression levels of GNGT1 of various cancers in TCGA database. (B,C) Expression of GNGT1 in LUAD tissues versus adjacent tissues in (B) GSE30219 and (C) GSE10072. (D) Relative expression levels of mRNA in frozen LUAD tissues and adjacent tissues of patients. (E) Clinical pathological features of GNGT1 expression in TCGA. (F) TP53 mutant status of GNGT1 expression in TCGA. (G) Overall survival analysis of GSE41271 according to GNGT1 expression. (H) Expression levels of GNGT1 protein in formalin-fixed tissues of patients with LUAD with paired normal tissues. (I,J) Fisher exact test of the relationship between GNGT1 immunohistochemical expression and the clinicopathological characteristics of patients with LUAD. ns, P>0.05; *, 0.01<P<0.05; **, 0.001<P<0.05; ***, P<0.001. TPM, transcripts per kilobase million; NSCLC, non-small cell lung cancer; CI, confidence interval; H&E, hematoxylin and eosin; IHC, immunohistochemistry; LUAD, lung adenocarcinoma; TCGA, The Cancer Genome Atlas; mRNA, messenger RNA.

Journal: Translational Lung Cancer Research

Article Title: GNGT1 remodels the tumor microenvironment and promotes immune escape through enhancing tumor stemness and modulating the fibrinogen beta chain-neutrophil extracellular trap signaling axis in lung adenocarcinoma

doi: 10.21037/tlcr-2024-1200

Figure Lengend Snippet: GNGT1 expression levels and clinical pathological features in LUAD. (A) Expression levels of GNGT1 of various cancers in TCGA database. (B,C) Expression of GNGT1 in LUAD tissues versus adjacent tissues in (B) GSE30219 and (C) GSE10072. (D) Relative expression levels of mRNA in frozen LUAD tissues and adjacent tissues of patients. (E) Clinical pathological features of GNGT1 expression in TCGA. (F) TP53 mutant status of GNGT1 expression in TCGA. (G) Overall survival analysis of GSE41271 according to GNGT1 expression. (H) Expression levels of GNGT1 protein in formalin-fixed tissues of patients with LUAD with paired normal tissues. (I,J) Fisher exact test of the relationship between GNGT1 immunohistochemical expression and the clinicopathological characteristics of patients with LUAD. ns, P>0.05; *, 0.01

Article Snippet: Rabbit anti-GNGT1 antibodies (cat. no. bs-13470R; Bioss Antibodies, Beijing, China) were diluted to 1:200, and IF staining was performed and scanned by Servicebio (Wuhan, China).

Techniques: Expressing, Mutagenesis, Immunohistochemical staining, Immunohistochemistry

The function analysis of GNGT1 in LUAD. (A) Volcano map of transcriptome analysis of DEGs between the GNGT1 high and low group. (B) Analysis of the top 5 genes associated with GNGT1. (C,D) Expression of the top 5 genes associated with GNGT1 in (C) LUAD and (D) LUSC according to GNGT1 expression. (E,F) GO and KEGG enrichment analysis of GNGT1 associated with (E) upregulation and (F) downregulation in biological pathways. (G) GSEA of genes associated with GNGT1 high expression versus those associated genes with low expression. (H) Relevance of GNGT1 across 14 functional states in difference cancers of cancer in the CancerSEA database. (I,J) The correlation between GNGT1 and functional states (metastasis, inflammation, and quiescence) in LUAD. *, 0.01<P<0.05; **, 0.001<P<0.05; ***, P<0.001. mRNA, messenger RNA; TPM, transcripts per kilobase million; BP, biological process; MF, molecular function; KEGG, Kyoto Encyclopedia of Genes and Genomes; CC, cellular component; NES, normalized enrichment score; FDR, false discovery rate; LUAD, lung adenocarcinoma; DEGs, differentially expressed genes; LUSC, lung squamous cell carcinoma; GO, Gene Ontology; GSEA, gene set enrichment analysis; BRCA, breast cancer; GBM, gioblastoma; OV, ovarian cancer; RB, retinoblastoma; UM, uveal melanoma; EMT, epithelial-mesenchymal transition.

Journal: Translational Lung Cancer Research

doi: 10.21037/tlcr-2024-1200

Figure Lengend Snippet: The function analysis of GNGT1 in LUAD. (A) Volcano map of transcriptome analysis of DEGs between the GNGT1 high and low group. (B) Analysis of the top 5 genes associated with GNGT1. (C,D) Expression of the top 5 genes associated with GNGT1 in (C) LUAD and (D) LUSC according to GNGT1 expression. (E,F) GO and KEGG enrichment analysis of GNGT1 associated with (E) upregulation and (F) downregulation in biological pathways. (G) GSEA of genes associated with GNGT1 high expression versus those associated genes with low expression. (H) Relevance of GNGT1 across 14 functional states in difference cancers of cancer in the CancerSEA database. (I,J) The correlation between GNGT1 and functional states (metastasis, inflammation, and quiescence) in LUAD. *, 0.01

Article Snippet: Rabbit anti-GNGT1 antibodies (cat. no. bs-13470R; Bioss Antibodies, Beijing, China) were diluted to 1:200, and IF staining was performed and scanned by Servicebio (Wuhan, China).

Techniques: Expressing, Functional Assay

Construction and verification of genetically modified GNGT1 fl/+ mice. (A) Pattern plot of conditional overexpression of GNGT1 in lung tissue. (B) Flowchart for obtaining GNGT1 fl/+ mice. (C) Expression level of GNGT1 mRNA in multiple organs (lung, liver, kidney, and colon) of GNGT1 fl/+ and wild-type mice. (D) Morphologic structure and GNGT1 expression of different organs in GNGT1 fl/+ and wild-type mice. (E-G) Expression of cell proliferation markers (E) Ki-67, (F) PCNA, (G) and apoptosis marker caspase-3 in the lungs of GNGT1 fl/+ and wild-type mice. ***, P<0.001. ATG, translation initiation codon ATG; Cre, cyclization recombination enzyme; mRNA, messenger RNA; WT, wild type; H&E, hematoxylin and eosin; IHC, immunohistochemistry; PCNA, proliferating cell nuclear antigen.

Journal: Translational Lung Cancer Research

doi: 10.21037/tlcr-2024-1200

Figure Lengend Snippet: Construction and verification of genetically modified GNGT1 fl/+ mice. (A) Pattern plot of conditional overexpression of GNGT1 in lung tissue. (B) Flowchart for obtaining GNGT1 fl/+ mice. (C) Expression level of GNGT1 mRNA in multiple organs (lung, liver, kidney, and colon) of GNGT1 fl/+ and wild-type mice. (D) Morphologic structure and GNGT1 expression of different organs in GNGT1 fl/+ and wild-type mice. (E-G) Expression of cell proliferation markers (E) Ki-67, (F) PCNA, (G) and apoptosis marker caspase-3 in the lungs of GNGT1 fl/+ and wild-type mice. ***, P<0.001. ATG, translation initiation codon ATG; Cre, cyclization recombination enzyme; mRNA, messenger RNA; WT, wild type; H&E, hematoxylin and eosin; IHC, immunohistochemistry; PCNA, proliferating cell nuclear antigen.

Article Snippet: Rabbit anti-GNGT1 antibodies (cat. no. bs-13470R; Bioss Antibodies, Beijing, China) were diluted to 1:200, and IF staining was performed and scanned by Servicebio (Wuhan, China).

Techniques: Genetically Modified, Over Expression, Expressing, Marker, Immunohistochemistry

High expression of GNGT1 promoted stemness gene expression in the lung. (A) The correlation analysis between GNGT1 and the stemness genes of LUAD in TCGA. (B) RT-qPCR and (C) immunofluorescence analysis results of stemness gene expression in lung tissues of wild-type and GNGT1 fl/+ mice (×400 magnification). **, 0.001<P<0.05; ***, P<0.001. TPM, transcripts per kilobase million; mRNA, messenger RNA; WT, wild type; DAPI, 4',6-diamidino-2-phenylindole; LUAD, lung adenocarcinoma; TCGA, The Cancer Genome Atlas; RT-qPCR, real-time quantitative polymerase chain reaction.

Journal: Translational Lung Cancer Research

doi: 10.21037/tlcr-2024-1200

Figure Lengend Snippet: High expression of GNGT1 promoted stemness gene expression in the lung. (A) The correlation analysis between GNGT1 and the stemness genes of LUAD in TCGA. (B) RT-qPCR and (C) immunofluorescence analysis results of stemness gene expression in lung tissues of wild-type and GNGT1 fl/+ mice (×400 magnification). **, 0.001

Article Snippet: Rabbit anti-GNGT1 antibodies (cat. no. bs-13470R; Bioss Antibodies, Beijing, China) were diluted to 1:200, and IF staining was performed and scanned by Servicebio (Wuhan, China).

Techniques: Expressing, Quantitative RT-PCR, Immunofluorescence, Real-time Polymerase Chain Reaction

Functional verification of GNGT1 in regulating tumor cell function and reshaping the tumor microenvironment. (A) The correlation analysis between GNGT1 and common driver genes of LUAD in TCGA. (B) Heatmap of the correlation between GNGT1 and driver genes. (C) RT-qPCR validation of driver gene expression level in the wild-type and GNGT1 fl/+ mouse models. (D) Venn diagram of GNGT1-related hub genes calculated by cytohubba app in Cytoscape. (E) Heatmap and (F) respective correlation analysis chart between GNGT1 and the obtained key genes. (G) RT-qPCR validation of hub genes level in the wild-type and GNGT1 fl/+ mouse models. (H) Model map of FGB-induced NET release and the function of NETs in regulating tumor function and remodeling the microenvironment. (I) qPCR analysis results of the expression of NET-related genes (PADI3, PADI4, and ELANE), three inflammation markers (IL-6, CXCL4, and CXCL15), and the molecules associated with tumor matrix remodeling (HMGB-1 and MMP9). ns, P>0.05; *, 0.01<P<0.05; **, 0.001<P<0.05; ***, P<0.001. TPM, transcripts per kilobase million; mRNA, messenger RNA; WT, wild type; MCC, maximal clique centrality; EPC, edge percolated component; FGB, fibrinogen beta chain; ALB, albumin; CTAG2, cancer/testis antigen 2; AFP, alpha-fetoprotein; HP, haptoglobin; FGF4, fibroblast growth factor 4; CXCL4, platelet factor 4; CXCL15, chemokine (C-X-C motif) ligand 15; HGMB-1, high mobility group protein B1; MMP9, matrix metallopeptidase 9; PAD, peptidyl arginine deiminase; ELANE, elastase; NET, neutrophils extracellular trap; TME, tumor microenvironment; EMT, epithelial-mesenchymal transition; LUAD, lung adenocarcinoma; TCGA, The Cancer Genome Atlas; RT-qPCR, real time quantitative polymerase chain reaction.

Journal: Translational Lung Cancer Research

doi: 10.21037/tlcr-2024-1200

Figure Lengend Snippet: Functional verification of GNGT1 in regulating tumor cell function and reshaping the tumor microenvironment. (A) The correlation analysis between GNGT1 and common driver genes of LUAD in TCGA. (B) Heatmap of the correlation between GNGT1 and driver genes. (C) RT-qPCR validation of driver gene expression level in the wild-type and GNGT1 fl/+ mouse models. (D) Venn diagram of GNGT1-related hub genes calculated by cytohubba app in Cytoscape. (E) Heatmap and (F) respective correlation analysis chart between GNGT1 and the obtained key genes. (G) RT-qPCR validation of hub genes level in the wild-type and GNGT1 fl/+ mouse models. (H) Model map of FGB-induced NET release and the function of NETs in regulating tumor function and remodeling the microenvironment. (I) qPCR analysis results of the expression of NET-related genes (PADI3, PADI4, and ELANE), three inflammation markers (IL-6, CXCL4, and CXCL15), and the molecules associated with tumor matrix remodeling (HMGB-1 and MMP9). ns, P>0.05; *, 0.01

Article Snippet: Rabbit anti-GNGT1 antibodies (cat. no. bs-13470R; Bioss Antibodies, Beijing, China) were diluted to 1:200, and IF staining was performed and scanned by Servicebio (Wuhan, China).

Techniques: Functional Assay, Cell Function Assay, Quantitative RT-PCR, Expressing, Real-time Polymerase Chain Reaction

Immune infiltration analysis in LUAD and the association with GNGT1 expression. (A) The correlation between GNGT1 gene alteration and the infiltration of different immune cells according to TIMER. (B-D) The correlation between GNGT1 expression and the infiltration of different immune cells as calculated by R software. (E) Immunohistochemical detection of common immune cell markers in LUAD with different GNGT1 expression levels, T-lymphocyte markers CD8 and CD4, neutrophil marker MPO, and B-lymphocyte marker CD20 (red arrow: immune cells; blue arrow: MPO staining of neutrophils was positive). *, 0.01<P<0.05; **, 0.001<P<0.05; ***, P<0.001. LUAD, lung adenocarcinoma; aDC, activated dendritic cell; DC, dendritic cells; iDC, immature dendritic cells; NK, natural killer; pDC, plasmacytoid dendritic cells; TPM, transcripts per kilobase million; H&E, hematoxylin and eosin; MPO, myeloperoxidase; TIMER, Tumor Immune Estimation Resource.

Journal: Translational Lung Cancer Research

doi: 10.21037/tlcr-2024-1200

Figure Lengend Snippet: Immune infiltration analysis in LUAD and the association with GNGT1 expression. (A) The correlation between GNGT1 gene alteration and the infiltration of different immune cells according to TIMER. (B-D) The correlation between GNGT1 expression and the infiltration of different immune cells as calculated by R software. (E) Immunohistochemical detection of common immune cell markers in LUAD with different GNGT1 expression levels, T-lymphocyte markers CD8 and CD4, neutrophil marker MPO, and B-lymphocyte marker CD20 (red arrow: immune cells; blue arrow: MPO staining of neutrophils was positive). *, 0.01

Article Snippet: Rabbit anti-GNGT1 antibodies (cat. no. bs-13470R; Bioss Antibodies, Beijing, China) were diluted to 1:200, and IF staining was performed and scanned by Servicebio (Wuhan, China).

Techniques: Expressing, Software, Immunohistochemical staining, Marker, Staining

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