aqp3  (Bioss)

Journal: Renal Failure

Article Title: Zhenwu decoction ameliorates adriamycin-induced nephrotic syndrome in rats via dual modulation of AVP-V2R-AQP2 and RAAS-MR-AQP3 pathways

doi: 10.1080/0886022X.2025.2599570

Figure Lengend Snippet: ZWD attenuates renal water reabsorption via suppressing AQPs in NS. (A-B) Location of AQP1, AQP2, and AQP3 in cortex (A) and medulla (B), detected by IHC. (C) Protein levels of AQP1, AQP2, and AQP3, detected by WB ( n = 6). (D-F) mRNA levels of AQP1, AQP2, and AQP3 detected by RT-qPCR ( n = 6). Model group vs. control group (** p < 0.01, ANOVA followed by LSD); ZWD and prednisone groups vs. model group (# p < 0.05, ## p < 0.01, ANOVA followed by LSD). AQP: aquaporin; WB: western blot; ANOVA: analysis of variance.

Article Snippet: The slices were blocked with 10% goat serum at room temperature and incubated with the primary antibodies against AQP1 (diluted 1:100, NB600-749, NOVUS, USA), AQP2 (diluted 1:100, DF7560, Affinity, USA), and AQP3 (diluted 1:1000, BS-1253R, BIOSS, China) were added and incubated at 4 °C overnight.

Techniques: Quantitative RT-PCR, Control, Western Blot

Journal: Renal Failure

Article Title: Zhenwu decoction ameliorates adriamycin-induced nephrotic syndrome in rats via dual modulation of AVP-V2R-AQP2 and RAAS-MR-AQP3 pathways

doi: 10.1080/0886022X.2025.2599570

Figure Lengend Snippet: Compound–major target molecular docking analysis. (A) Initial 3D conformation of unoptimized bioactive compounds. (B) 3D conformation of optimized bioactive compounds. (C) Interaction mode of AQP1 with benzoylpaeoniflorin. (D–F) Interaction mode of AQP2, V2R, and MR with 6-gingerol. (G) Interaction mode of AQP3 with paeoniflorin. AQP: aquaporin; V2R: type 2 vasopressin receptor; MR: mineralocorticoid receptor.

Article Snippet: The slices were blocked with 10% goat serum at room temperature and incubated with the primary antibodies against AQP1 (diluted 1:100, NB600-749, NOVUS, USA), AQP2 (diluted 1:100, DF7560, Affinity, USA), and AQP3 (diluted 1:1000, BS-1253R, BIOSS, China) were added and incubated at 4 °C overnight.

Techniques:

Journal: Frontiers in Cell and Developmental Biology

Article Title: Comparative analysis of AQP7 expression and cryotolerance in X- and Y-chromosome bearing bovine sperm

doi: 10.3389/fcell.2025.1582961

Figure Lengend Snippet: Western blot analysis of AQP3 in X and Y sperm (a) Western blot results; (b) Relative expression levels of AQP3 in different groups.

Article Snippet: After blocking with 5% non-fat milk, the membranes were incubated with primary antibodies against AQP3 (1:3,000, bs-1253R; Bioss, China) and AQP7 (1:3,000, bs-2506R; Bioss) overnight at 4°C.

Techniques: Western Blot, Expressing

Journal: Frontiers in Cell and Developmental Biology

Article Title: Comparative analysis of AQP7 expression and cryotolerance in X- and Y-chromosome bearing bovine sperm

doi: 10.3389/fcell.2025.1582961

Figure Lengend Snippet: Subcellular localization of AQP3 Sperm nuclei are stained with blue fluorescence, while AQP3 is visualized with red fluorescence (scale bar = 10 μm).

Article Snippet: After blocking with 5% non-fat milk, the membranes were incubated with primary antibodies against AQP3 (1:3,000, bs-1253R; Bioss, China) and AQP7 (1:3,000, bs-2506R; Bioss) overnight at 4°C.

Techniques: Staining, Fluorescence