Journal: Frontiers in Oncology
Article Title: Wan-Nian-Qing, a Herbal Composite Prescription, Suppresses the Progression of Liver Cancer in Mice by Regulating Immune Response
doi: 10.3389/fonc.2021.696282
Figure Lengend Snippet: Nrf2 signaling is involved in the improvement of immune function during WNQP-mediated inhibition of tumor growth. WNQP strongly enhanced the expression levels of Nrf2, HO-1, HO-2 and SOD-1 in the spleen tissues of BALB/c mice bearing (A, B) HepG2- and (C, D) SMMC-7721-xenograft tumors. (E, F) In Nrf2-siRNA-transfected primary cultured spleen cells, WNQP-induced increase in the levels of Nrf2, HO-1, HO-2 and SOD-1 were abolished. Quantitative protein expression data were normalized to the corresponding GAPDH levels, and the average fold changes in band intensity are marked. Data are expressed as the mean ± SD ( n = 3). * P < 0.05, ** P < 0.01 and *** P < 0.001 versus the control group.
Article Snippet: Subsequently, the membranes were blocked and incubated with the appropriate primary antibodies: bcl2-associated X (Bax) (21 kDa) (ab32503), B-cell lymphoma-extra-large (Bcl-xL) (26 kDa) (ab32370; Abcam, Cambridge, MA, USA), Nrf2 (97 kDa) (12721S; Cell Signaling Technologies, Danvers, MA, USA), heat shock protein 27 (HSP27) (27 kDa) (bs-0730R), heat shock protein 60 (HSP60) (58 kDa) (bs-0191R), heat shock protein 70 (HSP70) (70 kDa) (bs-0126R), HO-1 (32 kDa) (bs-2075R), HO-2 (36 kDa) (bs-1238R), SOD-1 (17 kDa) (bs-10216R), GAPDH (37 kDa) (bs-0755R; Bioss Antibodies, China), overnight at 4°C.
Techniques: Inhibition, Expressing, Transfection, Cell Culture
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