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rabbit antihuman zic1 polyclonal antibod (Bioss)

Name: rabbit antihuman zic1 polyclonal antibod
Brand: Bioss
Rating: 91 (5 reviews)

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Bioss rabbit antihuman zic1 polyclonal antibod
Rabbit Antihuman Zic1 Polyclonal Antibod, supplied by Bioss, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit antihuman zic1 polyclonal antibod/product/Bioss
Average 91 stars, based on 5 article reviews

rabbit antihuman zic1 polyclonal antibod - by Bioz Stars, 2026-02

91/100 stars

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Expression of ZIC1 mRNA in biopsy tissues. (A) Expression of ZIC1 in normal tissues, CIN and CSCC samples determined by RT-qPCR. (B) Expression of ZIC1 in normal tissues, CIN I, II and III, and CSCC samples determined by RT-qPCR. **P<0.01; ***P<0.001. ZIC1, zinc finger of the cerebellum 1; CSCC, cervical squamous cell carcinoma; CIN, cervical intraepithelial neoplasia; RT-qPCR, reverse transcription-quantitative PCR.

Journal: Oncology Letters

Article Title: Prognostic and clinicopathological value of ZIC1 in patients with cervical squamous cell carcinoma

doi: 10.3892/ol.2019.11007

Figure Lengend Snippet: Expression of ZIC1 mRNA in biopsy tissues. (A) Expression of ZIC1 in normal tissues, CIN and CSCC samples determined by RT-qPCR. (B) Expression of ZIC1 in normal tissues, CIN I, II and III, and CSCC samples determined by RT-qPCR. **P<0.01; ***P<0.001. ZIC1, zinc finger of the cerebellum 1; CSCC, cervical squamous cell carcinoma; CIN, cervical intraepithelial neoplasia; RT-qPCR, reverse transcription-quantitative PCR.

Article Snippet: The ZIC1 rabbit polyclonal antibody (1:200; cat. no. bs-11609R; BIOSS) was used as the primary antibody.

Techniques: Expressing, Quantitative RT-PCR, Real-time Polymerase Chain Reaction

Expression of ZIC1 protein in CIN III samples, CSCC and adjacent noncancerous tissues. Hematoxylin and eosin staining of (A) noncancerous tissues, (C) CIN III and (E) CSCC tissues (magnification ×100). Immunohistochemistry staining for ZIC1 protein (B) in noncancerous tissues, (D) CIN III and (F) CSCC tissues (magnification ×400). (G) There was a negative correlation between ZIC1 expression in CSCC tissues and expression in adjacent noncancerous tissues. (H) Statistical comparison of IRS of ZIC1 staining in CSCC, noncancerous tissues and CIN III samples. *P<0.05, ***P<0.001. CSCC, cervical squamous cell carcinoma; N, corresponding adjacent noncancerous tissues; CIN, cervical intraepithelial neoplasia; ZIC1, zinc finger of the cerebellum 1; IRS, immunoreactivity score.

Journal: Oncology Letters

Article Title: Prognostic and clinicopathological value of ZIC1 in patients with cervical squamous cell carcinoma

doi: 10.3892/ol.2019.11007

Figure Lengend Snippet: Expression of ZIC1 protein in CIN III samples, CSCC and adjacent noncancerous tissues. Hematoxylin and eosin staining of (A) noncancerous tissues, (C) CIN III and (E) CSCC tissues (magnification ×100). Immunohistochemistry staining for ZIC1 protein (B) in noncancerous tissues, (D) CIN III and (F) CSCC tissues (magnification ×400). (G) There was a negative correlation between ZIC1 expression in CSCC tissues and expression in adjacent noncancerous tissues. (H) Statistical comparison of IRS of ZIC1 staining in CSCC, noncancerous tissues and CIN III samples. *P<0.05, ***P<0.001. CSCC, cervical squamous cell carcinoma; N, corresponding adjacent noncancerous tissues; CIN, cervical intraepithelial neoplasia; ZIC1, zinc finger of the cerebellum 1; IRS, immunoreactivity score.

Article Snippet: The ZIC1 rabbit polyclonal antibody (1:200; cat. no. bs-11609R; BIOSS) was used as the primary antibody.

Techniques: Expressing, Staining, Immunohistochemistry

Association between ZIC1 expression in cervical squamous cell carcinoma and clinicopathological variables of patients.

Journal: Oncology Letters

Article Title: Prognostic and clinicopathological value of ZIC1 in patients with cervical squamous cell carcinoma

doi: 10.3892/ol.2019.11007

Figure Lengend Snippet: Association between ZIC1 expression in cervical squamous cell carcinoma and clinicopathological variables of patients.

Article Snippet: The ZIC1 rabbit polyclonal antibody (1:200; cat. no. bs-11609R; BIOSS) was used as the primary antibody.

Techniques: Expressing

Kaplan-Meier survival curves of patients with cervical squamous cell carcinoma stratified by high or low expression of ZIC1. (A) Overall survival and (B) disease-free survival. Blue lines represent the low ZIC1 expression group and the green lines represent the high ZIC1 expression group. ZIC1, zinc finger of the cerebellum 1.

Journal: Oncology Letters

Article Title: Prognostic and clinicopathological value of ZIC1 in patients with cervical squamous cell carcinoma

doi: 10.3892/ol.2019.11007

Figure Lengend Snippet: Kaplan-Meier survival curves of patients with cervical squamous cell carcinoma stratified by high or low expression of ZIC1. (A) Overall survival and (B) disease-free survival. Blue lines represent the low ZIC1 expression group and the green lines represent the high ZIC1 expression group. ZIC1, zinc finger of the cerebellum 1.

Article Snippet: The ZIC1 rabbit polyclonal antibody (1:200; cat. no. bs-11609R; BIOSS) was used as the primary antibody.

Techniques: Expressing

Prognostic value of ZIC1 expression and clinicopathological factors for overall survival of patients with cervical squamous cell carcinoma.

Journal: Oncology Letters

Article Title: Prognostic and clinicopathological value of ZIC1 in patients with cervical squamous cell carcinoma

doi: 10.3892/ol.2019.11007

Figure Lengend Snippet: Prognostic value of ZIC1 expression and clinicopathological factors for overall survival of patients with cervical squamous cell carcinoma.

Article Snippet: The ZIC1 rabbit polyclonal antibody (1:200; cat. no. bs-11609R; BIOSS) was used as the primary antibody.

Techniques: Expressing

Prognostic value of ZIC1 expression and clinicopathological factors for disease-free survival of patients with cervical squamous cell carcinoma.

Journal: Oncology Letters

Article Title: Prognostic and clinicopathological value of ZIC1 in patients with cervical squamous cell carcinoma

doi: 10.3892/ol.2019.11007

Figure Lengend Snippet: Prognostic value of ZIC1 expression and clinicopathological factors for disease-free survival of patients with cervical squamous cell carcinoma.

Article Snippet: The ZIC1 rabbit polyclonal antibody (1:200; cat. no. bs-11609R; BIOSS) was used as the primary antibody.

Techniques: Expressing

The PCR primers for the IAPs (Survivin and livin), eIF3 subunits, NM23 genes, ZIC family genes and GAPDH

Journal: Journal of Breast Cancer

Article Title: High Survivin and Low Zinc Finger of the Cerebellum 1 Expression Indicates Poor Prognosis in Triple-negative Breast Carcinoma

doi: 10.4048/jbc.2019.22.e20

Figure Lengend Snippet: The PCR primers for the IAPs (Survivin and livin), eIF3 subunits, NM23 genes, ZIC family genes and GAPDH

Article Snippet: Survivin (bs-0615R) and ZIC1 (bs-11609R) rabbit polyclonal antibodies (BIOSS, Beijing, China) were both diluted at a concentration of 1:200 in phosphate-buffered saline (PBS).

Techniques:

Associations of Survivin or ZIC1 expression with clinicopathological features of 150 patients with TNBC

Journal: Journal of Breast Cancer

Article Title: High Survivin and Low Zinc Finger of the Cerebellum 1 Expression Indicates Poor Prognosis in Triple-negative Breast Carcinoma

doi: 10.4048/jbc.2019.22.e20

Figure Lengend Snippet: Associations of Survivin or ZIC1 expression with clinicopathological features of 150 patients with TNBC

Article Snippet: Survivin (bs-0615R) and ZIC1 (bs-11609R) rabbit polyclonal antibodies (BIOSS, Beijing, China) were both diluted at a concentration of 1:200 in phosphate-buffered saline (PBS).

Techniques: Expressing

Associations of Survivin positive and ZIC1 negative expression with clinicopathological features of 150 patients with TNBC

Journal: Journal of Breast Cancer

Article Title: High Survivin and Low Zinc Finger of the Cerebellum 1 Expression Indicates Poor Prognosis in Triple-negative Breast Carcinoma

doi: 10.4048/jbc.2019.22.e20

Figure Lengend Snippet: Associations of Survivin positive and ZIC1 negative expression with clinicopathological features of 150 patients with TNBC

Article Snippet: Survivin (bs-0615R) and ZIC1 (bs-11609R) rabbit polyclonal antibodies (BIOSS, Beijing, China) were both diluted at a concentration of 1:200 in phosphate-buffered saline (PBS).

Techniques: Expressing

Prognostic role of Survivin, ZIC1 expression and clinicopathological features for the overall survival of TNBC patients evaluated using univariate and multivariate analyses with Cox regression

Journal: Journal of Breast Cancer

Article Title: High Survivin and Low Zinc Finger of the Cerebellum 1 Expression Indicates Poor Prognosis in Triple-negative Breast Carcinoma

doi: 10.4048/jbc.2019.22.e20

Figure Lengend Snippet: Prognostic role of Survivin, ZIC1 expression and clinicopathological features for the overall survival of TNBC patients evaluated using univariate and multivariate analyses with Cox regression

Article Snippet: Survivin (bs-0615R) and ZIC1 (bs-11609R) rabbit polyclonal antibodies (BIOSS, Beijing, China) were both diluted at a concentration of 1:200 in phosphate-buffered saline (PBS).

Techniques: Expressing

Prognostic role of Survivin, ZIC1 expression and clinicopathological features for the disease-free survival of TNBC patients evaluate using univariate and multivariate analyses with Cox regression

Journal: Journal of Breast Cancer

Article Title: High Survivin and Low Zinc Finger of the Cerebellum 1 Expression Indicates Poor Prognosis in Triple-negative Breast Carcinoma

doi: 10.4048/jbc.2019.22.e20

Figure Lengend Snippet: Prognostic role of Survivin, ZIC1 expression and clinicopathological features for the disease-free survival of TNBC patients evaluate using univariate and multivariate analyses with Cox regression

Article Snippet: Survivin (bs-0615R) and ZIC1 (bs-11609R) rabbit polyclonal antibodies (BIOSS, Beijing, China) were both diluted at a concentration of 1:200 in phosphate-buffered saline (PBS).

Techniques: Expressing

Prognostic role of Survivin combined with ZIC1 expression and clinicopathological features for the OS and DFS of TNBC patients evaluated using multivariate analyses with Cox regression

Journal: Journal of Breast Cancer

Article Title: High Survivin and Low Zinc Finger of the Cerebellum 1 Expression Indicates Poor Prognosis in Triple-negative Breast Carcinoma

doi: 10.4048/jbc.2019.22.e20

Figure Lengend Snippet: Prognostic role of Survivin combined with ZIC1 expression and clinicopathological features for the OS and DFS of TNBC patients evaluated using multivariate analyses with Cox regression

Article Snippet: Survivin (bs-0615R) and ZIC1 (bs-11609R) rabbit polyclonal antibodies (BIOSS, Beijing, China) were both diluted at a concentration of 1:200 in phosphate-buffered saline (PBS).

Techniques: Expressing

The expression of ZIC1 and survivin in 120 female invasive breast tumors was examined by immunohistochemistry (magnification, ×400). (A) Expression of ZIC1 in breast carcinoma; (B) expression of ZIC1 in corresponding adjacent normal tissues; (C) expression of survivin in breast carcinoma; (D) expression of survivin in corresponding adjacent normal tissues; (E) IRS scores of ZIC1 and survivin expression in breast tumors and corresponding adjacent normal tissues *** P<0.001; (F) Correlation analysis of ZIC1 and survivin expression in breast tumors.

Journal: International Journal of Oncology

Article Title: ZIC1 acts a tumor suppressor in breast cancer by targeting survivin

doi: 10.3892/ijo.2018.4450

Figure Lengend Snippet: The expression of ZIC1 and survivin in 120 female invasive breast tumors was examined by immunohistochemistry (magnification, ×400). (A) Expression of ZIC1 in breast carcinoma; (B) expression of ZIC1 in corresponding adjacent normal tissues; (C) expression of survivin in breast carcinoma; (D) expression of survivin in corresponding adjacent normal tissues; (E) IRS scores of ZIC1 and survivin expression in breast tumors and corresponding adjacent normal tissues *** P<0.001; (F) Correlation analysis of ZIC1 and survivin expression in breast tumors.

Article Snippet: ZIC1 (1:500; bs-11609R; Bioss Biotechnology, Beijing, China), survivin (RLM3419), Akt (RLT0178), p-Akt (RLP0006), mTOR (RLT2915), p-mTOR (RLP0176), cleaved caspase-9 (RLC0012), cleaved caspase-3 (RLC0004) (all 1:1,000; Suzhou Ruying Biotechnology, Suzhou, China), cyclin D1 (bs-20596R, 1:1,000; Bioss Biotechnology), p21 (YT3497), p27 (YT3501) (both 1:500; ImmunoWay Biotechnology, Piano, TX, USA), P70S6K (2708S), p-P70S6K (9204S), Bcl-2 (15071S), Bad (9268S), p-Bad (9291S) and Bcl-xL (2764S) (all 1:1,000) were used as the primary antibodies, and GAPDH (5174S, 1:1,000) (both from Cell Signaling Technology, Danvers, MA, USA) was used as the reference protein.

Techniques: Expressing, Immunohistochemistry

The expression of ZIC1 and survivin in 20 pairs of fresh-frozen invasive breast tumors and 7 cell lines was examined by western blot analysis. (A) Blots of ZIC1 and survivin expression in representative samples; (B) Relative protein expression of ZIC1 in 20 pairs of breast tumors was obviously lower than that in matched normal tissues, while expression of survivin in breast tumors was obviously higher than that in matched normal tissues; N, normal tissue; T, breast tumor tissue, *** P<0.001; (C) Blots of ZIC1 and survivin expression in 6 breast cancer cell lines and MCF-10A normal mammary epithelial cells; (D) Relative protein expression of ZIC1 in 6 breast cancer cell lines was obviously lower than that in MCF-10A normal cells, while expression of survivin in breast cancer cell lines was obviously higher than that in MCF-10A normal cells, *** P<0.001.

Journal: International Journal of Oncology

Article Title: ZIC1 acts a tumor suppressor in breast cancer by targeting survivin

doi: 10.3892/ijo.2018.4450

Figure Lengend Snippet: The expression of ZIC1 and survivin in 20 pairs of fresh-frozen invasive breast tumors and 7 cell lines was examined by western blot analysis. (A) Blots of ZIC1 and survivin expression in representative samples; (B) Relative protein expression of ZIC1 in 20 pairs of breast tumors was obviously lower than that in matched normal tissues, while expression of survivin in breast tumors was obviously higher than that in matched normal tissues; N, normal tissue; T, breast tumor tissue, *** P<0.001; (C) Blots of ZIC1 and survivin expression in 6 breast cancer cell lines and MCF-10A normal mammary epithelial cells; (D) Relative protein expression of ZIC1 in 6 breast cancer cell lines was obviously lower than that in MCF-10A normal cells, while expression of survivin in breast cancer cell lines was obviously higher than that in MCF-10A normal cells, *** P<0.001.

Techniques: Expressing, Western Blot

Associations of ZIC1/survivin expression with various clinicopathological characteristics of the 120 patients with invasive breast cancer.

Journal: International Journal of Oncology

Article Title: ZIC1 acts a tumor suppressor in breast cancer by targeting survivin

doi: 10.3892/ijo.2018.4450

Figure Lengend Snippet: Associations of ZIC1/survivin expression with various clinicopathological characteristics of the 120 patients with invasive breast cancer.

Techniques: Expressing

Analyses of ZIC1 and survivin in 1,075 TCGA RNA breast cancer samples. (A) ZIC1 fragments per kilobase million (FPKM) were obviously lower than survivin FPKM in 1,075 samples, *** P<0.001; (B) Left graph shows Kaplan-Meier curves for breast cancer according to ZIC1 expression. The prognosis of patients with a high ZIC1 expression (n=799) was better than that of those with a low ZIC1 expression (n=276) (P=0.013). Right graph shows Kaplan-Meier curves for breast cancer according to survivin expression. No significant difference was detected between the prognosis for patients with a high survivin expression (n=536) and those with a low survivin expression (n=539) (P=0.080).

Journal: International Journal of Oncology

Article Title: ZIC1 acts a tumor suppressor in breast cancer by targeting survivin

doi: 10.3892/ijo.2018.4450

Figure Lengend Snippet: Analyses of ZIC1 and survivin in 1,075 TCGA RNA breast cancer samples. (A) ZIC1 fragments per kilobase million (FPKM) were obviously lower than survivin FPKM in 1,075 samples, *** P<0.001; (B) Left graph shows Kaplan-Meier curves for breast cancer according to ZIC1 expression. The prognosis of patients with a high ZIC1 expression (n=799) was better than that of those with a low ZIC1 expression (n=276) (P=0.013). Right graph shows Kaplan-Meier curves for breast cancer according to survivin expression. No significant difference was detected between the prognosis for patients with a high survivin expression (n=536) and those with a low survivin expression (n=539) (P=0.080).

Techniques: Expressing

Assessment of lentiviral transfection in breast cancer cells. (A) MDA-MB-231 (upper diagram) and SK-BR3 (low diagram) transfected with rLV-ZsGreen-PGK-Puro in a bright field microscope (left panels: magnification, ×100) and green fluorescence microscope (right panels: magnification, ×100). (B) Ectopic expression of ZIC1 and survivin in MDA-MB-231 and SK-BR3 cells was examined by western blot analysis. (C) Relative protein expression of ZIC1 in the 'ZIC1' groups was obviously higher than that in the 'Vector' groups, while the expression of survivin in the 'ZIC1' groups (Z) was obviously lower than that in the 'Vector' groups (V), *** P<0.001; (D) The 2 −ΔΔCq value of ZIC1 in the 'ZIC1' groups was markedly higher than that in the 'Vector' groups, while the 2 −ΔΔCq value of survivin in the 'ZIC1' groups was markedly lower than that in the 'Vector' groups, *** P<0.001.

Journal: International Journal of Oncology

Article Title: ZIC1 acts a tumor suppressor in breast cancer by targeting survivin

doi: 10.3892/ijo.2018.4450

Figure Lengend Snippet: Assessment of lentiviral transfection in breast cancer cells. (A) MDA-MB-231 (upper diagram) and SK-BR3 (low diagram) transfected with rLV-ZsGreen-PGK-Puro in a bright field microscope (left panels: magnification, ×100) and green fluorescence microscope (right panels: magnification, ×100). (B) Ectopic expression of ZIC1 and survivin in MDA-MB-231 and SK-BR3 cells was examined by western blot analysis. (C) Relative protein expression of ZIC1 in the 'ZIC1' groups was obviously higher than that in the 'Vector' groups, while the expression of survivin in the 'ZIC1' groups (Z) was obviously lower than that in the 'Vector' groups (V), *** P<0.001; (D) The 2 −ΔΔCq value of ZIC1 in the 'ZIC1' groups was markedly higher than that in the 'Vector' groups, while the 2 −ΔΔCq value of survivin in the 'ZIC1' groups was markedly lower than that in the 'Vector' groups, *** P<0.001.

Techniques: Transfection, Microscopy, Fluorescence, Expressing, Western Blot, Plasmid Preparation

ZIC1 suppresses cell proliferation, reduces mitochondrial membrane potential and promotes apoptosis in breast cancer. (A) The viability of stably transfected breast cancer cells was determined by MTT cell proliferation assay; * P<0.05, ** P<0.01 and *** P<0.001. (B) The effects of ZIC1 on cell growth were confirmed by colony formation assay (left diagrams show representative images and the right graph shows the quantitative analysis of colony numbers), ** P<0.01 and *** P<0.001. (C) Cell cycle distributions were detected by flow cytometric analysis in stably transfected breast cancer cells. (D) Blots of Cyto-c expression in mitochondria and cytosol of stably transfected cells. (E) Relative protein expression of Cyto-c expression in mitochondria and cytosol of stably transfected cells, ** P<0.01 and *** P<0.001. (F) Cell apoptotic rates were detected by flow cytometry in stably transfected breast cancer cells.

Journal: International Journal of Oncology

Article Title: ZIC1 acts a tumor suppressor in breast cancer by targeting survivin

doi: 10.3892/ijo.2018.4450

Figure Lengend Snippet: ZIC1 suppresses cell proliferation, reduces mitochondrial membrane potential and promotes apoptosis in breast cancer. (A) The viability of stably transfected breast cancer cells was determined by MTT cell proliferation assay; * P<0.05, ** P<0.01 and *** P<0.001. (B) The effects of ZIC1 on cell growth were confirmed by colony formation assay (left diagrams show representative images and the right graph shows the quantitative analysis of colony numbers), ** P<0.01 and *** P<0.001. (C) Cell cycle distributions were detected by flow cytometric analysis in stably transfected breast cancer cells. (D) Blots of Cyto-c expression in mitochondria and cytosol of stably transfected cells. (E) Relative protein expression of Cyto-c expression in mitochondria and cytosol of stably transfected cells, ** P<0.01 and *** P<0.001. (F) Cell apoptotic rates were detected by flow cytometry in stably transfected breast cancer cells.

Techniques: Stable Transfection, Transfection, MTT Cell Proliferation, Colony Assay, Expressing, Flow Cytometry

ZIC1 inhibits tumor formation in vivo . (A) Empty vector and ZIC1 transfectant tumors; (B) growth curves of empty vector and ZIC1 transfectant tumors; (C) expression of ZIC1 and survivin in empty vector and ZIC1 transfectant tumors was examined by immunohistochemistry (magnification, ×400).

Journal: International Journal of Oncology

Article Title: ZIC1 acts a tumor suppressor in breast cancer by targeting survivin

doi: 10.3892/ijo.2018.4450

Figure Lengend Snippet: ZIC1 inhibits tumor formation in vivo . (A) Empty vector and ZIC1 transfectant tumors; (B) growth curves of empty vector and ZIC1 transfectant tumors; (C) expression of ZIC1 and survivin in empty vector and ZIC1 transfectant tumors was examined by immunohistochemistry (magnification, ×400).

Techniques: In Vivo, Plasmid Preparation, Transfection, Expressing, Immunohistochemistry

The moleculular mechanisms of action of ZIC1 in breast cancer. (A) Proteins of the Akt/mTOR/P70S6K pathway, cell cycle regulatory proteins and apoptosis-related proteins in MDA-MB-231 and SK-BR3 breast cancer cells with or without ectopic expression of ZIC1 were investigated by western blot analysis. (B) Schematic model of the role of ZIC1 in breast cancer: ZIC1 inhibits survivin expression by the blockade of the Akt/mTOR/P70S6K signaling pathway; the reduced expression of survivin and the ectopic expression of ZIC1 induce cell cycle arrest at the G1 phase together; ZIC1 releases Cyto-c from the mitochondria and then promotes the apoptosis of breast cancer cells; and reduced expression of survivin diminished the suppression of cleaved caspase-9 and cleaved caspase-3.

Journal: International Journal of Oncology

Article Title: ZIC1 acts a tumor suppressor in breast cancer by targeting survivin

doi: 10.3892/ijo.2018.4450

Figure Lengend Snippet: The moleculular mechanisms of action of ZIC1 in breast cancer. (A) Proteins of the Akt/mTOR/P70S6K pathway, cell cycle regulatory proteins and apoptosis-related proteins in MDA-MB-231 and SK-BR3 breast cancer cells with or without ectopic expression of ZIC1 were investigated by western blot analysis. (B) Schematic model of the role of ZIC1 in breast cancer: ZIC1 inhibits survivin expression by the blockade of the Akt/mTOR/P70S6K signaling pathway; the reduced expression of survivin and the ectopic expression of ZIC1 induce cell cycle arrest at the G1 phase together; ZIC1 releases Cyto-c from the mitochondria and then promotes the apoptosis of breast cancer cells; and reduced expression of survivin diminished the suppression of cleaved caspase-9 and cleaved caspase-3.

Techniques: Expressing, Western Blot

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