cd45  (Bioss)

Journal: bioRxiv

Article Title: 4-Methylumbelliferone Restores Age-Related Changes in Perineuronal Nets, Memory and Neuroinflammation

doi: 10.64898/2026.01.30.702721

Figure Lengend Snippet: (A) Representative images of CD45 (red), Iba1 (green) and DAPI (blue) staining in the indicated regions. Arrows mark CD45 + Iba1 − cells and the insets show a magnified view of one such cell. (B) Quantification of CD45 + Iba1 − cells per mm 2 in the indicated areas, showing increased number of PICs in the corpus callosum with aging, which was reduced to young adult levels after 4-MU treatment. Data are presented as mean ± SD and were analyzed using ordinary one-way ANOVA; **p < 0.01, **** p < 0.0001.

Article Snippet: Sections were incubated overnight at 4 °C with primary antibodies against GFAP (chicken, 1:800, ab4674, Abcam), Iba1 (goat, 1:800, 019-19741, Fujifilm), CD45 (rabbit, 1:300, bs-10599R, Bioss), and biotinylated WFA.

Techniques: Staining

Journal: Scientific Reports

Article Title: Dynamics of CD44 + bovine nucleus pulposus cells with inflammation

doi: 10.1038/s41598-024-59504-7

Figure Lengend Snippet: Co-expression analysis of CD44, CD45 and CD14 in NP cells in response to a pro-inflammatory/degenerative stimulus. ( A ) Representative images of sagittal sections of IVD explants with immunofluorescence staining for CD44, CD45 and CD14 in bovine NPs after 2 days in culture (scale bar: 50 μm). ( B ) Quantification of the percentage of cells simultaneously expressing CD44, CD45 and CD14 markers was performed, as previously described ( n = 4). Results are presented as box and whiskers plots with representation of median, min and max. Statistical analysis was performed and p values indicated in the plots.

Article Snippet: Following three washing steps with PBS + 0.5% Tween-20, tissue sections were incubated with the primary antibodies: mouse anti-CD14 (1:200, Bio-Rad, clone CC-G33), rabbit anti-CD45 (1:50, Bioss, polyclonal) and rat anti-CD44 (1:50, Thermofisher, clone Hermes-1), diluted in Antibody Diluent with Background Reduction Solution (Dako) overnight at 4 °C in a humid environment.

Techniques: Expressing, Immunofluorescence, Staining

Journal: Frontiers in Immunology

Article Title: Single-cell RNA sequencing reveals the immune microenvironment and signaling networks in cystitis glandularis

doi: 10.3389/fimmu.2023.1083598

Figure Lengend Snippet: Classification and analysis of T cells in cystitis glandularis. (A) U-MAP plot showing different T cell subsets indicated by color. (B) Dot plot showing canonical genes used to distinguish different subsets of T cells. (C) The proportion of cells of different subsets of T cells in normal and CG tissues. The left panel indicates normal tissue and the right panel indicates inflamed tissue. (D) Heat map showing functional gene sets of different T-cell subsets. (E, F) Monocle2 inferences of the pseudotime trajectory of T cells. Above: pseudotime trajectory of CD4+ T cells/CD8+ T cells of all samples annotated with different colors or color depth, respectively. Bottom: Trends of expression of activated and terminated gene sets in CD4+ T cells/CD8+ T cells along with pseudotime trajectory. The gene sets used can be seen in supplementary table S5. (G) GSVA enrichment analysis of NK cells in CG and normal tissues. The horizontal axis shows the enrichment score, the right side shows the CG enrichment function, and the left side shows the normal tissue enrichment function. This figure displays only part of the Inflammation related pathway – see supplementary table S7 for all enrichment results. (H) Heat map showing the regulation of transcription factors within T cells. (I) Multiplex immunofluorescence showing the expression of the IL2RB-pSTAT5-FOXP3 activation pathway in Treg cells. The red arrow indicates the Treg cells activated by this activation pathway.

Article Snippet: A18036), PIGR (ABclonal, A61301), KRT19 (ABclonal, A19040), KRT15 (ABclonal, A4854), KRT13 (ABclonal, A0411), IL-2 (BIOSS, BS-1959R, CD8 (BIOSS, BS-10599R), FOXP3 (CST, 41822), P-STAT5 (BIOSS, BS-5703R), TNFR1A (ABclonal, A1540), NF-kB p65 (ABclonal, A11204), Vimentin (ABclonal, A19607), CD79a (ABclonal, A22452), CD27 (ABclonal, A11505), CD20 (ABclonal, A4893).

Techniques: Functional Assay, Expressing, Multiplex Assay, Immunofluorescence, Activation Assay