Journal: European Journal of Medical Research
Article Title: Invisible appliance promotes bone reconstruction via modulating the periodontal immune microenvironment
doi: 10.1186/s40001-025-02503-9
Figure Lengend Snippet: Effect of different treatment modalities on Treg-related transcription factor—Foxp3. A Representative immunohistochemical staining images (positive staining for biomarkers (indicated by arrows) red to brown in color) and statistical analysis of Foxp3. B mRNA expression level of Foxp3 detected by qRT-PCR. In the same treatment, * P < 0.05, ** P < 0.01 vs. control; # P < 0.05, ## P < 0.01 vs. model; & P < 0.05, && P < 0.01 vs. scaling-0 day. In the comparison of different treatment, * P < 0.05, ** P < 0.01 vs. no appliance; # P < 0.05, ## P < 0.01 vs. fixed appliance. n = 4 per group
Article Snippet: Afterwards, the slices were incubated with goat serum for 15 min and treated with the following primary antibodies at 4 °C overnight: anti-FOXP3 (No.bs-10211R, Bioss, Woburn, MA, USA), anti-RANKL (No.bs-0747R, Bioss), anti-RORγt (No.bs23110R, Bioss), and anti-OPG (No.A2100, ABclonal, Woburn, MA, USA).
Techniques: Immunohistochemical staining, Staining, Expressing, Quantitative RT-PCR, Control, Comparison