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nanog (Bioss)

Name: nanog
Brand: Bioss
Rating: 91 (11 reviews)

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Fig. 2 <t>High</t> <t>TC2N</t> expression represents the upregulated expression of stem cell markers in lung cancer, but not in normal lung. A Gene set enrichment analysis (GSEA) analysis of TCGA database revealed that TC2N was related to “LUNG CELL DIFFERENTIATION” process in lung cancer but not in normal lung. NES, Normalized Enrichment Score. B Cohort 1 showed that the protein expression of TC2N was associated to differentiation degree (well, moderately and poorly differentiated) of human lung tumor. Scale bar represent 100 μm. The P value was measured with Student’s t-tests. **P < 0.01, ***P < 0.001. C Cohort 2 verified that the protein expression of TC2N was associated to DD of human lung tumor. The P value was measured with Student’s t-tests. ***P < 0.001. D Kaplan–Meier and COX regression analyses showed that TC2N expression was associated with poor prognosis of lung cancer patients. E Survival analysis of TC2N expression in lung cancer patients with well tumor differentiation by Kaplan–Meier and COX regression analyses. F Survival analysis of TC2N expression in lung cancer patients with moderately tumor differentiation by Kaplan–Meier and COX regression analyses. G Survival analysis of TC2N expression in lung cancer patients with poorly tumor differentiation by Kaplan–Meier and COX regression analyses. H IHC evaluation of SOX2, OCT4 and <t>NANOG</t> expression in normal lung tissues of TC2N engineered mice without urethane exposure. Scale bar represent 100 μm. I IHC evaluation of SOX2, OCT4 and NANOG expression in lung tissues of TC2N engineered mice with urethane exposure. Scale bar represent 100 μm

Nanog, supplied by Bioss, used in various techniques. Bioz Stars score: 91/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 11 article reviews

nanog - by Bioz Stars, 2026-02

91/100 stars

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1) Product Images from "TC2N maintains stem cell-like characteristics to accelerate lung carcinogenesis by blockade of dual specificity protein phosphatase 3."

Article Title: TC2N maintains stem cell-like characteristics to accelerate lung carcinogenesis by blockade of dual specificity protein phosphatase 3.

Journal: Cell & bioscience

doi: 10.1186/s13578-025-01348-3

Fig. 2 High TC2N expression represents the upregulated expression of stem cell markers in lung cancer, but not in normal lung. A Gene set enrichment analysis (GSEA) analysis of TCGA database revealed that TC2N was related to “LUNG CELL DIFFERENTIATION” process in lung cancer but not in normal lung. NES, Normalized Enrichment Score. B Cohort 1 showed that the protein expression of TC2N was associated to differentiation degree (well, moderately and poorly differentiated) of human lung tumor. Scale bar represent 100 μm. The P value was measured with Student’s t-tests. **P < 0.01, ***P < 0.001. C Cohort 2 verified that the protein expression of TC2N was associated to DD of human lung tumor. The P value was measured with Student’s t-tests. ***P < 0.001. D Kaplan–Meier and COX regression analyses showed that TC2N expression was associated with poor prognosis of lung cancer patients. E Survival analysis of TC2N expression in lung cancer patients with well tumor differentiation by Kaplan–Meier and COX regression analyses. F Survival analysis of TC2N expression in lung cancer patients with moderately tumor differentiation by Kaplan–Meier and COX regression analyses. G Survival analysis of TC2N expression in lung cancer patients with poorly tumor differentiation by Kaplan–Meier and COX regression analyses. H IHC evaluation of SOX2, OCT4 and NANOG expression in normal lung tissues of TC2N engineered mice without urethane exposure. Scale bar represent 100 μm. I IHC evaluation of SOX2, OCT4 and NANOG expression in lung tissues of TC2N engineered mice with urethane exposure. Scale bar represent 100 μm

Figure Legend Snippet: Fig. 2 High TC2N expression represents the upregulated expression of stem cell markers in lung cancer, but not in normal lung. A Gene set enrichment analysis (GSEA) analysis of TCGA database revealed that TC2N was related to “LUNG CELL DIFFERENTIATION” process in lung cancer but not in normal lung. NES, Normalized Enrichment Score. B Cohort 1 showed that the protein expression of TC2N was associated to differentiation degree (well, moderately and poorly differentiated) of human lung tumor. Scale bar represent 100 μm. The P value was measured with Student’s t-tests. **P < 0.01, ***P < 0.001. C Cohort 2 verified that the protein expression of TC2N was associated to DD of human lung tumor. The P value was measured with Student’s t-tests. ***P < 0.001. D Kaplan–Meier and COX regression analyses showed that TC2N expression was associated with poor prognosis of lung cancer patients. E Survival analysis of TC2N expression in lung cancer patients with well tumor differentiation by Kaplan–Meier and COX regression analyses. F Survival analysis of TC2N expression in lung cancer patients with moderately tumor differentiation by Kaplan–Meier and COX regression analyses. G Survival analysis of TC2N expression in lung cancer patients with poorly tumor differentiation by Kaplan–Meier and COX regression analyses. H IHC evaluation of SOX2, OCT4 and NANOG expression in normal lung tissues of TC2N engineered mice without urethane exposure. Scale bar represent 100 μm. I IHC evaluation of SOX2, OCT4 and NANOG expression in lung tissues of TC2N engineered mice with urethane exposure. Scale bar represent 100 μm

Techniques Used: Expressing, Cell Differentiation

Fig. 3 TC2N upregulates the CSC-like characteristics of lung cancer cells. A Gene set enrichment analysis (GSEA) analysis of TCGA database revealed that TC2N was related to “STEM CELL” relevant processes in lung cancer but not in normal lung. NES, Normalized Enrichment Score. B Cellular morphol ogy of adherent lung cancer cells (Ad) compared to spheroid-forming lung cancer cells (Sp). Scale bars represent 120 μm (upper). WB analysis of TC2N expression in adherent and spheroid‐forming lung cancer cells (lower). C WB analysis of SOX2, OCT4 and NANOG expression in stable lung cancer cells. D The sphere formation ability of lung cancer cells with TC2N overexpression (TC2N group) and knockdown (shRNA group) was compared to that of the negative control (NC group). Scale bars represent 120 μm. Mean ± SEM. (n = 3). The P value was measured with Student’s t-tests. *P < 0.05, **P < 0.01. E The expression of CSCs marker CD133 (APC) in lung cancer cells with ectopic expression of TC2N (NC, shRNA and TC2N groups) were analyzed by flow cytometry. Mean ± SEM. (n = 3). The P value was measured with Student’s t-tests. **P < 0.01, ***P < 0.001. F The proportion of side population cells in lung cancer cells with ectopic expression of TC2N (NC, shRNA and TC2N groups) were analyzed by flow cytometry. Mean ± SEM. (n = 3). The P value was mea sured with Student’s t-tests. **P < 0.01, ***P < 0.001. G Soft agar colony formation ability of spheroid‐forming lung cancer cells with ectopic expression of TC2N (NC, shRNA and TC2N groups). Mean ± SEM. (n = 3). The P value was measured with Student’s t-tests. **P < 0.01, ***P < 0.001. The cell experiments were repeated 3 times independently

Figure Legend Snippet: Fig. 3 TC2N upregulates the CSC-like characteristics of lung cancer cells. A Gene set enrichment analysis (GSEA) analysis of TCGA database revealed that TC2N was related to “STEM CELL” relevant processes in lung cancer but not in normal lung. NES, Normalized Enrichment Score. B Cellular morphol ogy of adherent lung cancer cells (Ad) compared to spheroid-forming lung cancer cells (Sp). Scale bars represent 120 μm (upper). WB analysis of TC2N expression in adherent and spheroid‐forming lung cancer cells (lower). C WB analysis of SOX2, OCT4 and NANOG expression in stable lung cancer cells. D The sphere formation ability of lung cancer cells with TC2N overexpression (TC2N group) and knockdown (shRNA group) was compared to that of the negative control (NC group). Scale bars represent 120 μm. Mean ± SEM. (n = 3). The P value was measured with Student’s t-tests. *P < 0.05, **P < 0.01. E The expression of CSCs marker CD133 (APC) in lung cancer cells with ectopic expression of TC2N (NC, shRNA and TC2N groups) were analyzed by flow cytometry. Mean ± SEM. (n = 3). The P value was measured with Student’s t-tests. **P < 0.01, ***P < 0.001. F The proportion of side population cells in lung cancer cells with ectopic expression of TC2N (NC, shRNA and TC2N groups) were analyzed by flow cytometry. Mean ± SEM. (n = 3). The P value was mea sured with Student’s t-tests. **P < 0.01, ***P < 0.001. G Soft agar colony formation ability of spheroid‐forming lung cancer cells with ectopic expression of TC2N (NC, shRNA and TC2N groups). Mean ± SEM. (n = 3). The P value was measured with Student’s t-tests. **P < 0.01, ***P < 0.001. The cell experiments were repeated 3 times independently

Techniques Used: Expressing, Over Expression, Knockdown, shRNA, Negative Control, Marker, Flow Cytometry

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Journal: Cell & bioscience

Article Title: TC2N maintains stem cell-like characteristics to accelerate lung carcinogenesis by blockade of dual specificity protein phosphatase 3.

doi: 10.1186/s13578-025-01348-3

Figure Lengend Snippet: Fig. 2 High TC2N expression represents the upregulated expression of stem cell markers in lung cancer, but not in normal lung. A Gene set enrichment analysis (GSEA) analysis of TCGA database revealed that TC2N was related to “LUNG CELL DIFFERENTIATION” process in lung cancer but not in normal lung. NES, Normalized Enrichment Score. B Cohort 1 showed that the protein expression of TC2N was associated to differentiation degree (well, moderately and poorly differentiated) of human lung tumor. Scale bar represent 100 μm. The P value was measured with Student’s t-tests. **P < 0.01, ***P < 0.001. C Cohort 2 verified that the protein expression of TC2N was associated to DD of human lung tumor. The P value was measured with Student’s t-tests. ***P < 0.001. D Kaplan–Meier and COX regression analyses showed that TC2N expression was associated with poor prognosis of lung cancer patients. E Survival analysis of TC2N expression in lung cancer patients with well tumor differentiation by Kaplan–Meier and COX regression analyses. F Survival analysis of TC2N expression in lung cancer patients with moderately tumor differentiation by Kaplan–Meier and COX regression analyses. G Survival analysis of TC2N expression in lung cancer patients with poorly tumor differentiation by Kaplan–Meier and COX regression analyses. H IHC evaluation of SOX2, OCT4 and NANOG expression in normal lung tissues of TC2N engineered mice without urethane exposure. Scale bar represent 100 μm. I IHC evaluation of SOX2, OCT4 and NANOG expression in lung tissues of TC2N engineered mice with urethane exposure. Scale bar represent 100 μm

Article Snippet: Briefly, the protein expression of TC2N, DUSP3, SOX2, OCT4 and NANOG were detected using their primary antibodies: 1:200 diluted rabbit polyclonal antibody (Cat. #: HPA027549; Sigma) for TC2N; 1:500 diluted rabbit polyclonal antibody (Cat. #: bs-6246R; Bioss) for DUSP3; 1:1000 diluted rabbit polyclonal antibody (Cat. #: 11064-1-AP; Proteintech) for SOX2; 1:1000 diluted rabbit polyclonal antibody (Cat. #: 60242-1-Ig; Proteintech) for OCT4; 1:1000 diluted rabbit polyclonal antibody (Cat. #: 14295-1-AP; Proteintech) for NANOG; 1:200 diluted rabbit polyclonal antibody (Cat. #: 10490-1-AP; Proteintech) for CC10; 1:200 diluted rabbit polyclonal antibody (Cat. #: 10774-1-AP; Proteintech) for SFTPC.

Techniques: Expressing, Cell Differentiation

Journal: Cell & bioscience

Article Title: TC2N maintains stem cell-like characteristics to accelerate lung carcinogenesis by blockade of dual specificity protein phosphatase 3.

doi: 10.1186/s13578-025-01348-3

Figure Lengend Snippet: Fig. 3 TC2N upregulates the CSC-like characteristics of lung cancer cells. A Gene set enrichment analysis (GSEA) analysis of TCGA database revealed that TC2N was related to “STEM CELL” relevant processes in lung cancer but not in normal lung. NES, Normalized Enrichment Score. B Cellular morphol ogy of adherent lung cancer cells (Ad) compared to spheroid-forming lung cancer cells (Sp). Scale bars represent 120 μm (upper). WB analysis of TC2N expression in adherent and spheroid‐forming lung cancer cells (lower). C WB analysis of SOX2, OCT4 and NANOG expression in stable lung cancer cells. D The sphere formation ability of lung cancer cells with TC2N overexpression (TC2N group) and knockdown (shRNA group) was compared to that of the negative control (NC group). Scale bars represent 120 μm. Mean ± SEM. (n = 3). The P value was measured with Student’s t-tests. *P < 0.05, **P < 0.01. E The expression of CSCs marker CD133 (APC) in lung cancer cells with ectopic expression of TC2N (NC, shRNA and TC2N groups) were analyzed by flow cytometry. Mean ± SEM. (n = 3). The P value was measured with Student’s t-tests. **P < 0.01, ***P < 0.001. F The proportion of side population cells in lung cancer cells with ectopic expression of TC2N (NC, shRNA and TC2N groups) were analyzed by flow cytometry. Mean ± SEM. (n = 3). The P value was mea sured with Student’s t-tests. **P < 0.01, ***P < 0.001. G Soft agar colony formation ability of spheroid‐forming lung cancer cells with ectopic expression of TC2N (NC, shRNA and TC2N groups). Mean ± SEM. (n = 3). The P value was measured with Student’s t-tests. **P < 0.01, ***P < 0.001. The cell experiments were repeated 3 times independently

Techniques: Expressing, Over Expression, Knockdown, shRNA, Negative Control, Marker, Flow Cytometry

Journal: Advanced Science

Article Title: LIF Promotes Sec15b‐Mediated STAT3 Exosome Secretion to Maintain Stem Cell Pluripotency in Mouse Embryonic Development

doi: 10.1002/advs.202407971

Figure Lengend Snippet:

Article Snippet: Nanog , Bioss , Catbs‐0829R; RRID: AB_10857750.

Techniques:

List of antibodies used in Western blot assay.

Journal: International Journal of Molecular Sciences

Article Title: A Fungicide, Fludioxonil, Formed the Polyploid Giant Cancer Cells and Induced Metastasis and Stemness in MDA-MB-231 Triple-Negative Breast Cancer Cells

doi: 10.3390/ijms25169024

Figure Lengend Snippet: List of antibodies used in Western blot assay.

Article Snippet: Nanog , Bioss , 34 , bs-0829R , 1:10.

Techniques: Western Blot

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