Journal: Cellular and Molecular Gastroenterology and Hepatology
Article Title: Fibroblast Growth Factor 20 Attenuates Colitis by Restoring Impaired Intestinal Epithelial Barrier Integrity and Modulating Macrophage Polarization via S100A9 in an NF-κB-Dependent Manner
doi: 10.1016/j.jcmgh.2025.101486
Figure Lengend Snippet: FGF20 is markedly increased in patients and mice with colitis. ( A ) Box plot of FGF20 mRNA in healthy control animals and ulcerative colitis specimens (using dataset GSE22619). ( B-D ) The body weight, DAI scores, and gross morphology images of colons and colon lengths of each group were recorded (n ≥ 3). ( E ) hematoxylin and eosin (H&E)-stained colonic sections of each group mice. Scale bar, 100 μm and 50 μm (n ≥ 3). ( F, G ) Expression of FGF20 in the colon of each group of mice was detected by Western blot and immunohistochemistry (n ≥ 3). Scale bar, 50 μm and 20 μm. ( H ) Serum FGF20 contents were measured by enzyme-linked immunosorbent assay (n = 8). ( I ) Expression of FGFR1, FGFR2, FGFR3, and FGFR4 were detected by Western blot (n ≥ 3). Data are represented as mean ± standard error of the mean (SEM); ∗ P < .05, ∗∗ P < .01, ∗∗∗ P < .001.
Article Snippet: FGF20 (1/500, SC-373927, Santa CruzA), Bcl2 (1/1000, AB182858, Abcam), Bax (1/1000, AB32503, Abcam), S100A9 (1/500, SC-53187, Santa Cruz), S100A8 (1/500, SC-48352, Santa Cruz), phosphorylated (p) -IκBα (1/500, SC-8404, Santa Cruz), p-P65 (1/500, SC-136548, Santa Cruz), IκBα (1/1000, 4814S, CST, USA), P65 (1/1000, 4814S, CST), TNF-α (1/1000, AF7014, Affinity, China), IL6 (1/1000, 12912S, CST), FGFR1 (1/1000, bs-0230R, Bioss, China), FGFR2 (1/1000, bs-0675R, Bioss), FGFR3 (1/2000, MA5-31142, ThermoFisher), FGFR4 (1/1000, bsm-61509R, Bioss), GAPDH (1/10000, HRP-60004, Proteintech, China), and Beta-Tubulin (1/5000, 10094-1-AP, Proteintech).
Techniques: Control, Staining, Expressing, Western Blot, Immunohistochemistry, Enzyme-linked Immunosorbent Assay
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