Journal: Experimental physiology

Article Title: The parotid secretory protein BPIFA2 is a salivary surfactant that affects LPS action

doi: 10.1113/EP088567

Figure Lengend Snippet: Analysis of mouse saliva. A. Stimulated whole saliva from three WT and three Bpifa2 KO male mice was analyzed by SDS-PAGE and the proteins were visualized by Coomassie Blue stain. Molecular size standards (Mr, kD) are shown on the left and the expected positions of amylase (arrows) and BPIFA2 (arrowheads) are indicated. B. Detection of BPIFA2 in mouse saliva by immunoblotting. Saliva from three WT, Bpifa2 KO and heterozygous (Ht) mice, respectively, was analyzed with an antibody to mouse BPIFA2. The experiment was repeated with WT and Bpifa2 KO saliva with similar results.

Article Snippet: Bpifa2 KO mice have recently been phenotyped and the results posted by the International Mouse Phenotyping Consortium (IMPC) ( Brown et al., 2018 ).

Techniques: SDS Page, Staining, Western Blot

Journal: Experimental physiology

Article Title: The parotid secretory protein BPIFA2 is a salivary surfactant that affects LPS action

doi: 10.1113/EP088567

Figure Lengend Snippet: Saliva secretion. A. Pilocarpine-stimulated saliva was collected from male (M, squares) and female (F, circles), WT (closed symbols) and Bpifa2 KO (open symbols) mice. Saliva volume was expressed relative to body weight (µl saliva / g body weight). Data from two (M) or four (F) independent experiments are shown with the group means indicated by horizontal lines. WT and KO mice were compared by Student’s t-test for each sex (N=5–13).

Article Snippet: Bpifa2 KO mice have recently been phenotyped and the results posted by the International Mouse Phenotyping Consortium (IMPC) ( Brown et al., 2018 ).

Techniques:

Journal: Experimental physiology

Article Title: The parotid secretory protein BPIFA2 is a salivary surfactant that affects LPS action

doi: 10.1113/EP088567

Figure Lengend Snippet: Analysis of mouse saliva. A. Stimulated whole saliva from three WT and three Bpifa2 KO male mice was analyzed by SDS-PAGE and the proteins were visualized by Coomassie Blue stain. Molecular size standards (Mr, kD) are shown on the left and the expected positions of amylase (arrows) and BPIFA2 (arrowheads) are indicated. B. Detection of BPIFA2 in mouse saliva by immunoblotting. Saliva from three WT, Bpifa2 KO and heterozygous (Ht) mice, respectively, was analyzed with an antibody to mouse BPIFA2. The experiment was repeated with WT and Bpifa2 KO saliva with similar results.

Article Snippet: Generation of Bpifa2 knock-out mice.. Bpifa2 knockout mice (Bpifa2 tm1(KOMP)Vlcg ) (IMSR Cat# KOMP:VG14843-1-Vlcg, RRID:IMSR_KOMP:VG14843–1-Vlcg) were generated on a C57BL/6NTac background using ES colonies that were produced by Regeneron Pharmaceuticals, Inc. using the approach described by Regeneron ( http://velocigene.com/komp/detail/14843 ) ( Valenzuela et al., 2003 ).

Techniques: SDS Page, Staining, Western Blot

Journal: Experimental physiology

Article Title: The parotid secretory protein BPIFA2 is a salivary surfactant that affects LPS action

doi: 10.1113/EP088567

Figure Lengend Snippet: Saliva secretion. A. Pilocarpine-stimulated saliva was collected from male (M, squares) and female (F, circles), WT (closed symbols) and Bpifa2 KO (open symbols) mice. Saliva volume was expressed relative to body weight (µl saliva / g body weight). Data from two (M) or four (F) independent experiments are shown with the group means indicated by horizontal lines. WT and KO mice were compared by Student’s t-test for each sex (N=5–13).

Article Snippet: Generation of Bpifa2 knock-out mice.. Bpifa2 knockout mice (Bpifa2 tm1(KOMP)Vlcg ) (IMSR Cat# KOMP:VG14843-1-Vlcg, RRID:IMSR_KOMP:VG14843–1-Vlcg) were generated on a C57BL/6NTac background using ES colonies that were produced by Regeneron Pharmaceuticals, Inc. using the approach described by Regeneron ( http://velocigene.com/komp/detail/14843 ) ( Valenzuela et al., 2003 ).

Techniques: