Journal: International Journal of Molecular Sciences

Article Title: RNA-Seq Analysis of UPM-Exposed Epithelium Co-Cultivated with Macrophages and Dendritic Cells in Obstructive Lung Diseases

doi: 10.3390/ijms23169125

Figure Lengend Snippet: The list of genes chosen for qPCR validation.

Article Snippet: BPIFA2 , Hs00395980_m1 , , , 68.

Techniques: Biomarker Discovery, Activity Assay

Journal: International Journal of Molecular Sciences

Article Title: RNA-Seq Analysis of UPM-Exposed Epithelium Co-Cultivated with Macrophages and Dendritic Cells in Obstructive Lung Diseases

doi: 10.3390/ijms23169125

Figure Lengend Snippet: The overall p -value of the comparisons between the control, asthma, and COPD groups of mRNA expression of the selected genes in the UPM-exposed epithelium from the mono- and triple co-cultures.

Article Snippet: BPIFA2 , Hs00395980_m1 , , , 68.

Techniques: Control, Expressing

Journal: International Journal of Molecular Sciences

Article Title: RNA-Seq Analysis of UPM-Exposed Epithelium Co-Cultivated with Macrophages and Dendritic Cells in Obstructive Lung Diseases

doi: 10.3390/ijms23169125

Figure Lengend Snippet: The sequence of primers used in the PCR.

Article Snippet: BPIFA2 , Hs00395980_m1 , , , 68.

Techniques: Sequencing

Journal: International Journal of Molecular Sciences

Article Title: Differential Expression of Peroxisomal Proteins in Distinct Types of Parotid Gland Tumors

doi: 10.3390/ijms22157872

Figure Lengend Snippet: List of antibodies used for the detection of peroxisomal proteins in the parotid gland.

Article Snippet: Parotid secretory Protein, mouse (SPLUNC2) , Goat, polyclonal , 25 kDa , - , 1:3000 , Acris Antibodies, Inc. Cat. no.: AP31965PU-N.

Techniques: Molecular Weight, Marker

Journal: Experimental physiology

Article Title: The parotid secretory protein BPIFA2 is a salivary surfactant that affects LPS action

doi: 10.1113/EP088567

Figure Lengend Snippet: Analysis of mouse saliva. A. Stimulated whole saliva from three WT and three Bpifa2 KO male mice was analyzed by SDS-PAGE and the proteins were visualized by Coomassie Blue stain. Molecular size standards (Mr, kD) are shown on the left and the expected positions of amylase (arrows) and BPIFA2 (arrowheads) are indicated. B. Detection of BPIFA2 in mouse saliva by immunoblotting. Saliva from three WT, Bpifa2 KO and heterozygous (Ht) mice, respectively, was analyzed with an antibody to mouse BPIFA2. The experiment was repeated with WT and Bpifa2 KO saliva with similar results.

Article Snippet: Bpifa2 KO mice have recently been phenotyped and the results posted by the International Mouse Phenotyping Consortium (IMPC) ( Brown et al., 2018 ).

Techniques: SDS Page, Staining, Western Blot

Journal: Experimental physiology

Article Title: The parotid secretory protein BPIFA2 is a salivary surfactant that affects LPS action

doi: 10.1113/EP088567

Figure Lengend Snippet: Saliva secretion. A. Pilocarpine-stimulated saliva was collected from male (M, squares) and female (F, circles), WT (closed symbols) and Bpifa2 KO (open symbols) mice. Saliva volume was expressed relative to body weight (µl saliva / g body weight). Data from two (M) or four (F) independent experiments are shown with the group means indicated by horizontal lines. WT and KO mice were compared by Student’s t-test for each sex (N=5–13).

Article Snippet: Bpifa2 KO mice have recently been phenotyped and the results posted by the International Mouse Phenotyping Consortium (IMPC) ( Brown et al., 2018 ).

Techniques:

Journal: Experimental physiology

Article Title: The parotid secretory protein BPIFA2 is a salivary surfactant that affects LPS action

doi: 10.1113/EP088567

Figure Lengend Snippet: Analysis of mouse saliva. A. Stimulated whole saliva from three WT and three Bpifa2 KO male mice was analyzed by SDS-PAGE and the proteins were visualized by Coomassie Blue stain. Molecular size standards (Mr, kD) are shown on the left and the expected positions of amylase (arrows) and BPIFA2 (arrowheads) are indicated. B. Detection of BPIFA2 in mouse saliva by immunoblotting. Saliva from three WT, Bpifa2 KO and heterozygous (Ht) mice, respectively, was analyzed with an antibody to mouse BPIFA2. The experiment was repeated with WT and Bpifa2 KO saliva with similar results.

Article Snippet: Generation of Bpifa2 knock-out mice.. Bpifa2 knockout mice (Bpifa2 tm1(KOMP)Vlcg ) (IMSR Cat# KOMP:VG14843-1-Vlcg, RRID:IMSR_KOMP:VG14843–1-Vlcg) were generated on a C57BL/6NTac background using ES colonies that were produced by Regeneron Pharmaceuticals, Inc. using the approach described by Regeneron ( http://velocigene.com/komp/detail/14843 ) ( Valenzuela et al., 2003 ).

Techniques: SDS Page, Staining, Western Blot

Journal: Experimental physiology

Article Title: The parotid secretory protein BPIFA2 is a salivary surfactant that affects LPS action

doi: 10.1113/EP088567

Figure Lengend Snippet: Saliva secretion. A. Pilocarpine-stimulated saliva was collected from male (M, squares) and female (F, circles), WT (closed symbols) and Bpifa2 KO (open symbols) mice. Saliva volume was expressed relative to body weight (µl saliva / g body weight). Data from two (M) or four (F) independent experiments are shown with the group means indicated by horizontal lines. WT and KO mice were compared by Student’s t-test for each sex (N=5–13).

Article Snippet: Generation of Bpifa2 knock-out mice.. Bpifa2 knockout mice (Bpifa2 tm1(KOMP)Vlcg ) (IMSR Cat# KOMP:VG14843-1-Vlcg, RRID:IMSR_KOMP:VG14843–1-Vlcg) were generated on a C57BL/6NTac background using ES colonies that were produced by Regeneron Pharmaceuticals, Inc. using the approach described by Regeneron ( http://velocigene.com/komp/detail/14843 ) ( Valenzuela et al., 2003 ).

Techniques:

Journal: Cell reports

Article Title: Functional Specialization of Human Salivary Glands and Origins of Proteins Intrinsic to Human Saliva

doi: 10.1016/j.celrep.2020.108402

Figure Lengend Snippet: (A) Immunohistochemistry of glandular tissues. The SM and PAR acinar cells are highly enriched for amylase (AMY1), statherin (STATH), and lactoperoxidase (LPO) compared with the SL, consistent with these proteins being markers of serous cells. MUC7 and CRISP3 are expressed by a subset of acinar cells of the SM and SL, with little to no expression in the PAR. MUC5B is highly expressed by the SL mucous acinar cells, but not by the acinar cells of the PAR or SM, indicating it to be a marker of SL function. Scale bar, 25 μm. (B) MUC7 and amylase are expressed by distinct subtypes of serous acinar cells. The mature SM tissue section was immunolabeled for MUC7 (red), AMY (green), and ECAD (gray). (C) Gel electrophoretic separation of whole mouth saliva (WS) and glandular ductal secretions (PAR and SM/SL), followed by staining of proteins with Coomassie blue and of glycoproteins with periodic acid Schiff stain (pink bands in left panel) and probing of transfers with antibodies against MUC7, AMY, BPIFA2, CRISP3, and STATH (right panel).

Article Snippet: Rabbit polyclonal anti-BPIFA2 , Eurogentec , .

Techniques: Immunohistochemistry, Expressing, Marker, Immunolabeling, Staining

Journal: Cell reports

Article Title: Functional Specialization of Human Salivary Glands and Origins of Proteins Intrinsic to Human Saliva

doi: 10.1016/j.celrep.2020.108402

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal anti-BPIFA2 , Eurogentec , .

Techniques: Recombinant, Lysis, Bicinchoninic Acid Protein Assay, Software