anti bmp5  (Proteintech)

Journal: Frontiers in Endocrinology

Article Title: Amelioration of polycystic ovarian morphology by Tokishakuyakusan in a PCOS rat model: association with bone morphogenetic protein 4

doi: 10.3389/fendo.2025.1649124

Figure Lengend Snippet: Bmp4 , Bmp5 , and Inhba expression in the ovaries. (A–C) Relative mRNA expression quantified by real-time PCR. Expression levels are relative to 18S ribosomal RNA. Data are shown as mean ± standard deviation with individual data points. P -values were obtained by one-way analysis of variance (ANOVA) with Tukey’s post hoc test (vehicle: n = 6; DHT: n = 9; DHT+TSS: n = 10). (D–G) Correlation analysis between gene expression and ovarian follicle counts in the DHT and DHT+TSS groups ( n = 6 per group). The correlation coefficient ( r 2 ) and P -value were calculated. DHT, 5α-dihydrotestosterone; TSS, Tokishakuyakusan; Bmp, bone morphogenetic protein; Inhba, inhibin-βa; PCR, polymerase chain reaction.

Article Snippet: The following TaqMan primer–probe sets (Thermo Fisher Scientific) were used: Bmp4 , Rn00432087_m1; Bmp5 , Rn01447676_m1; inhibin-βa ( Inhba ), Rn01538592_m1; steroidogenic acute regulatory protein ( Star ), Rn00580695_m1; cytochrome P450 11A1 ( Cyp11a1 ), Rn00568733_m1; 3β hydroxysteroid dehydrogenase ( Hsd3b ), Rn01789220_m1; 18s , Hs99999901_s1; and Gapdh , Rn01775763_g1.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Standard Deviation, Gene Expression, Polymerase Chain Reaction

Journal: bioRxiv

Article Title: Vascular endothelial growth factor-D improves lung vascular integrity during acute lung injury

doi: 10.1101/2024.12.16.628787

Figure Lengend Snippet: A) NICHES analysis of AFs, mesothelial cells, pericytes, gCap, and aCap in human microvascular niche yields a quantitative cell-cell signaling atlas of AF-aCap, AF-gCap, Mesothelial-aCap, Mesothelial-gCap, Pericyte-aCap, and Pericyte-gCap visualized by low-dimensional UMAP embedding. B) FeaturePlot of top differentially expressed L/R pairs ANGPT1 - TEK , SLIT2 - ROBO4 , VEGFD - KDR , BMP5-BMPR2 , ADM - RAMP2 , WNT5A - FZD6 , PTN - RACK1 , OXT - EDNRB , and ADCYAP1 - VIPR1 from NICHES analysis C) Top 10 differentially expressed L/R pairs between different cell-cell interaction signals processed through NICHES and plotted in DoHeatmap. D) Immunostaining of representative L/R pairs from AF to microvascular endothelial cells, including SLIT2 - ROBO4 , ADM - RAMP2 , BMP5 - BMPR2 , and VEGFD - VEGFR2 in human lung section. ITGA8 is a marker for AF; PRX is a marker for microvascular endothelial cells. Scale bar 20 μm.

Article Snippet: The protein levels of SLIT2, ADM, BMP5, and VEGFD in samples harvested from different timepoints were determined using corresponding ELISA kits: SLIT2 (# NB030384), ADM (# NBP2-78738), BMP5 (# NBP2-69996), VEGFD (# NBP2-78890), all from Novus Biologicals.

Techniques: Immunostaining, Marker

Journal: bioRxiv

Article Title: Vascular endothelial growth factor-D improves lung vascular integrity during acute lung injury

doi: 10.1101/2024.12.16.628787

Figure Lengend Snippet: A, B) The electrical resistance and permeability in HLMVECs after treatment with VEGF-D (1 μg/mL), adrenomedullin (ADM)(100 ng/mL), angiopoietin 1 (ANGPT1)(2 μg/mL), BMP-5 (1 μg/mL), SLIT2 (1 μg/mL), CXCL6 (1 μg/mL), Pleiotrophin (PTN)(1 μg/mL), SEMA6D (1 μg/mL), and TNF-α (1 ng/mL) were measured through electrical cell impendence sensing (ECIS) assay, and transwell assay, respectively. C) HLMVECs cultured on transwell treated with VEGF-D, followed by challenging with IL1β, TNF-α, or thrombin. The permeability was determined using a 20kDa FITC-dextran probe. D) Immunofluorescent images of F actin (red), and VE-Cadherin (green) in HLMVECs challenged with TNF-α or IL1β with or without VEGF-D. White boxes highlight VE-Cadherin junctions in all conditions. Scale bar 20 μm. E) Protein expression of phosphor-MLC in HLMVECs challenged with thrombin with different concentrations of VEGF-D was assessed through a western blot. Data presented as mean ± SEM. * and ** indicate p<0.05 and p<0.01, respectively.

Article Snippet: The protein levels of SLIT2, ADM, BMP5, and VEGFD in samples harvested from different timepoints were determined using corresponding ELISA kits: SLIT2 (# NB030384), ADM (# NBP2-78738), BMP5 (# NBP2-69996), VEGFD (# NBP2-78890), all from Novus Biologicals.

Techniques: Permeability, Transwell Assay, Cell Culture, Expressing, Western Blot

Journal: bioRxiv

Article Title: Vascular endothelial growth factor-D improves lung vascular integrity during acute lung injury

doi: 10.1101/2024.12.16.628787

Figure Lengend Snippet: A) NICHES analysis of AFs, mesothelial cells, pericytes, gCap, and aCap in human microvascular niche yields a quantitative cell-cell signaling atlas of AF-aCap, AF-gCap, Mesothelial-aCap, Mesothelial-gCap, Pericyte-aCap, and Pericyte-gCap visualized by low-dimensional UMAP embedding. B) FeaturePlot of top differentially expressed L/R pairs ANGPT1 - TEK , SLIT2 - ROBO4 , VEGFD - KDR , BMP5-BMPR2 , ADM - RAMP2 , WNT5A - FZD6 , PTN - RACK1 , OXT - EDNRB , and ADCYAP1 - VIPR1 from NICHES analysis C) Top 10 differentially expressed L/R pairs between different cell-cell interaction signals processed through NICHES and plotted in DoHeatmap. D) Immunostaining of representative L/R pairs from AF to microvascular endothelial cells, including SLIT2 - ROBO4 , ADM - RAMP2 , BMP5 - BMPR2 , and VEGFD - VEGFR2 in human lung section. ITGA8 is a marker for AF; PRX is a marker for microvascular endothelial cells. Scale bar 20 μm.

Article Snippet: The following ligands were purchased from vendors: BMP5 (Novus Biologicals, # 615-BMC-020), VEGF-D (Cayman Chemical, #32055), Adrenomedullin (ADM)(Cayman Chemical, #24889), Angiopoietin-1 (Novus Biologicals, #923-AN), TNF-α (R&D Systems, # 10291-TA), Slit homolog 2 (SLIT2)(R&D Systems, # 8616-SL), CXCL6 (R&D Systems, # 333-GC-025/CF), Pleiotrophin (PTN)(R&D Systems, # 252-PL), and Semaphorin 6D (Sema6D)(R&D Systems, #2095-S6).

Techniques: Immunostaining, Marker