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OriGene gfp tagged orf plasmids
Gfp Tagged Orf Plasmids, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/birc3/cIAP2+(BIRC3)+(NM_182962)+Human+Tagged+ORF+Clone/pmc13183958-281-6-9
Average 94 stars, based on 1 article reviews

gfp tagged orf plasmids - by Bioz Stars, 2026-10

94/100 stars

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Expressing:

Article Title: NPD1-mediated stereoselective regulation of BIRC3 expression through cREL is decisive for neural cell survival.
Article Snippet: .. BIRC3 and cREL expression was silenced using a mixture of three siRNA commercially available as Trilencer-27 siRNA knockdown duplexes kit (Origene, Rockville, MD, USA). .. Wild-type BIRC3-, cREL- and cRELfused-with-GFP-expression constructs were obtained from Origene.

Article Title: NPD1-mediated stereoselective regulation of BIRC3 expression through cREL is decisive for neural cell survival
Article Snippet: .. BIRC3 and cREL expression was silenced using a mixture of three siRNA commercially available as Trilencer-27 siRNA knockdown duplexes kit (Origene, Rockville, MD, USA). .. Wild-type BIRC3-, cREL- and cREL-fused-with-GFP-expression constructs were obtained from Origene.

Knockdown:

Transfection:

Article Title: IL-1β induces up-regulation of BIRC3, a gene involved in chemoresistance to doxorubicin in breast cancer cells.
Article Snippet: Epithelial to mesenchymal transition (EMT) of tumor cells facilitates their progress to metastasis.. In the tumor microenvironment the inflammatory cytokine 1b (IL-1b) has been associated with tumor development and invasiveness.. IL-1b-induced EMT triggers the expression of markers associated with malignancy.

Image Search Results

Mean (logRQ ± SE) expression of BIRC3 and XAF1 genes depending on PD-1 expression, The U Mann Whitney Test.

Journal: Scientific Reports

Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

doi: 10.1038/s41598-026-35887-7

Figure Lengend Snippet: Mean (logRQ ± SE) expression of BIRC3 and XAF1 genes depending on PD-1 expression, The U Mann Whitney Test.

Article Snippet: The following TaqMan probes were used for the reaction: NAIP (Hs03037952_m1), BIRC2 (Hs00357350_m1), BIRC3 (Hs00154109_m1), XIAP (Hs00236913_m1), BIRC5 (Hs00153353_m1), BIRC6 (Hs00212288_m1), BIRC8 (Hs01057786), XAF1 (Hs01550138_m1), DIABLO (Hs00219876_m1), CASP3 (Hs00234387_m1) and CASP9 (Hs00962278_m1).

Techniques: Expressing, MANN-WHITNEY

Mean (logRQ ± SE) expression of BIRC2 (p = 0.025) , BIRC3 and BIRC5 genes depending on survival rate (< 20 months and > 20 months), (The U Mann Whitney Test)

Journal: Scientific Reports

Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

doi: 10.1038/s41598-026-35887-7

Figure Lengend Snippet: Mean (logRQ ± SE) expression of BIRC2 (p = 0.025) , BIRC3 and BIRC5 genes depending on survival rate (< 20 months and > 20 months), (The U Mann Whitney Test)

Techniques: Expressing, MANN-WHITNEY

Mean (logRQ ± SE) expression of BIRC2 and BIRC3 genes depending on survival rate (< 12 months and > 12 months), (The U Mann Whitney Test)

Journal: Scientific Reports

Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

doi: 10.1038/s41598-026-35887-7

Figure Lengend Snippet: Mean (logRQ ± SE) expression of BIRC2 and BIRC3 genes depending on survival rate (< 12 months and > 12 months), (The U Mann Whitney Test)

Techniques: Expressing, MANN-WHITNEY

Scatterplot between BIRC2 gene expression (logRQ) and survival rate ( r = -0.478 p < 0.05 Spearman’s rank correlations).

Journal: Scientific Reports

Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

doi: 10.1038/s41598-026-35887-7

Figure Lengend Snippet: Scatterplot between BIRC2 gene expression (logRQ) and survival rate ( r = -0.478 p < 0.05 Spearman’s rank correlations).

Techniques: Gene Expression

Scatterplot between BIRC3 gene expression (logRQ) and survival rate ( r = -0.536 p < 0.05 Spearman’s rank correlations).

Journal: Scientific Reports

Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

doi: 10.1038/s41598-026-35887-7

Figure Lengend Snippet: Scatterplot between BIRC3 gene expression (logRQ) and survival rate ( r = -0.536 p < 0.05 Spearman’s rank correlations).

Techniques: Gene Expression

Mean (logRQ ± SE) expression of BIRC2 and BIRC3 genes depending on progression free survival (< 20 months and > 20 months), (The U Mann Whitney Test)

Journal: Scientific Reports

Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

doi: 10.1038/s41598-026-35887-7

Figure Lengend Snippet: Mean (logRQ ± SE) expression of BIRC2 and BIRC3 genes depending on progression free survival (< 20 months and > 20 months), (The U Mann Whitney Test)

Techniques: Expressing, MANN-WHITNEY

Scatterplot between BIRC2 gene expression (logRQ) and progression-free survival ( r =-0.481 p < 0.05 Spearman’s rank correlations).

Journal: Scientific Reports

Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

doi: 10.1038/s41598-026-35887-7

Figure Lengend Snippet: Scatterplot between BIRC2 gene expression (logRQ) and progression-free survival ( r =-0.481 p < 0.05 Spearman’s rank correlations).

Techniques: Gene Expression

Mean (logRQ ± SE) expression of BIRC2 and BIRC3 genes depending on progression-free survival (< 12 months and > 12 months), (The U Mann Whitney Test)

Journal: Scientific Reports

Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

doi: 10.1038/s41598-026-35887-7

Figure Lengend Snippet: Mean (logRQ ± SE) expression of BIRC2 and BIRC3 genes depending on progression-free survival (< 12 months and > 12 months), (The U Mann Whitney Test)

Techniques: Expressing, MANN-WHITNEY

Scatterplot between BIRC3 gene expression (logRQ) and progression-free survival ( r =-0.536 p < 0.05 Spearman’s rank correlations).

Journal: Scientific Reports

Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

doi: 10.1038/s41598-026-35887-7

Figure Lengend Snippet: Scatterplot between BIRC3 gene expression (logRQ) and progression-free survival ( r =-0.536 p < 0.05 Spearman’s rank correlations).

Techniques: Gene Expression

Forest plot of odds ratios (OR) from univariate logistic regression for dichotomized survival endpoints (OS/PFS) in the intersection set, using BIRC2 expression (logRQ) as predictor.

Journal: Scientific Reports

Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

doi: 10.1038/s41598-026-35887-7

Figure Lengend Snippet: Forest plot of odds ratios (OR) from univariate logistic regression for dichotomized survival endpoints (OS/PFS) in the intersection set, using BIRC2 expression (logRQ) as predictor.

Techniques: Expressing

Journal: Scientific Reports

Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

doi: 10.1038/s41598-026-35887-7

Techniques: Expressing

Kaplan–Meier overall survival stratified by BIRC2 expression group (high vs. low). Log-rank p = 0.023* BIRC2 group (high = logRQ BIRC2 >-0.077794 (median) /low = logRQ BIRC2 <=-0.077794).

Journal: Scientific Reports

Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

doi: 10.1038/s41598-026-35887-7

Figure Lengend Snippet: Kaplan–Meier overall survival stratified by BIRC2 expression group (high vs. low). Log-rank p = 0.023* BIRC2 group (high = logRQ BIRC2 >-0.077794 (median) /low = logRQ BIRC2 <=-0.077794).

Techniques: Expressing

Kaplan–Meier overall survival stratified by BIRC3 expression group (high vs. low). Log-rank p = 0.1824. BIRC3 group (high = logRQ BIRC3 >-0.316210 (median) /low = logRQ BIRC3 <=-0.316210).

Journal: Scientific Reports

Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

doi: 10.1038/s41598-026-35887-7

Figure Lengend Snippet: Kaplan–Meier overall survival stratified by BIRC3 expression group (high vs. low). Log-rank p = 0.1824. BIRC3 group (high = logRQ BIRC3 >-0.316210 (median) /low = logRQ BIRC3 <=-0.316210).

Techniques: Expressing

Examples of PR regulated proteins and pathways involved in cell proliferation and apoptosis. The quantitative proteomic analysis identified significant changes of thousands of proteins and many hundreds of phosphorylated peptides in response to PR agonist. This figure shows proteins and pathways that were analyzed and interpreted in greater details. (A) Proteins and complexes involved in R5020 induced G0/G1 and G2/M cell cycle arrest, which could also be triggers for apoptosis. (B) PR regulated mitochondrial proteins involved in R5020 induced apoptosis. Significant increases of HIF1A and its downstream targets such as BNIP3 and NIX activated mitophagy together with upregulated LC3. Prolonged increase of BNIP3 and NIX causes mitochondria dysfunction and loss of mitochondria membrane potential. BNIP3 and NIX can sequester anti-apoptotic proteins such as BCL-2 and BCL-XL. This relieved BH3-only proteins, which facilitated formation of BAX and BAK pores on mitochondria outer membrane, leading to MOMPs, cytochrome c release and CASP9 activation, along with upregulation and releases of pro-apoptotic factors such as AIF, ENDOG, HtrA2/Omi, and Smac/DIABLO. PR induced apoptotic mechanism may also involve some of the 200 mitochondrial proteins regulated by R5020. (C) p53 pathway may also participate in R5020-induced cell apoptosis through upregulation of proteins such as death receptors FAS, DR4 and DR5. Upregulation of MDM2 is involved in a negative feedback regulation of p53. (D) TNF signaling via NFKB is generally pro-survival. It was found to be markedly upregulated. Gene silencing of its key players BIRC3 and TNFAIP3 alone or in combination had no influence on R5020 induced downregulation of effector caspases and apoptosis. (E) True to the holistic nature of proteomic analysis, the study also detected the previously reported upregulation of protooncogenic proteins by R5020, but the upregulation of these proteins are futile and should not be interpreted as evidence for pro-tumoral activity of PR. Additionally, all effector CASPs detected in the MS data and in immunoblots were downregulation despite R5020 induced apoptosis. *Although CASP6 is marginally increased in MS data and immunoblot, its activation by cleavage is evidently reduced in immunoblot. Blue downward arrow = Downregulation; Red upward arrow = Upregulation.

Journal: Scientific Reports

Article Title: Proteomics analysis reveals progesterone receptor induced mitochondria-mediated apoptosis in breast cancer cells

doi: 10.1038/s41598-025-28183-3

Figure Lengend Snippet: Examples of PR regulated proteins and pathways involved in cell proliferation and apoptosis. The quantitative proteomic analysis identified significant changes of thousands of proteins and many hundreds of phosphorylated peptides in response to PR agonist. This figure shows proteins and pathways that were analyzed and interpreted in greater details. (A) Proteins and complexes involved in R5020 induced G0/G1 and G2/M cell cycle arrest, which could also be triggers for apoptosis. (B) PR regulated mitochondrial proteins involved in R5020 induced apoptosis. Significant increases of HIF1A and its downstream targets such as BNIP3 and NIX activated mitophagy together with upregulated LC3. Prolonged increase of BNIP3 and NIX causes mitochondria dysfunction and loss of mitochondria membrane potential. BNIP3 and NIX can sequester anti-apoptotic proteins such as BCL-2 and BCL-XL. This relieved BH3-only proteins, which facilitated formation of BAX and BAK pores on mitochondria outer membrane, leading to MOMPs, cytochrome c release and CASP9 activation, along with upregulation and releases of pro-apoptotic factors such as AIF, ENDOG, HtrA2/Omi, and Smac/DIABLO. PR induced apoptotic mechanism may also involve some of the 200 mitochondrial proteins regulated by R5020. (C) p53 pathway may also participate in R5020-induced cell apoptosis through upregulation of proteins such as death receptors FAS, DR4 and DR5. Upregulation of MDM2 is involved in a negative feedback regulation of p53. (D) TNF signaling via NFKB is generally pro-survival. It was found to be markedly upregulated. Gene silencing of its key players BIRC3 and TNFAIP3 alone or in combination had no influence on R5020 induced downregulation of effector caspases and apoptosis. (E) True to the holistic nature of proteomic analysis, the study also detected the previously reported upregulation of protooncogenic proteins by R5020, but the upregulation of these proteins are futile and should not be interpreted as evidence for pro-tumoral activity of PR. Additionally, all effector CASPs detected in the MS data and in immunoblots were downregulation despite R5020 induced apoptosis. *Although CASP6 is marginally increased in MS data and immunoblot, its activation by cleavage is evidently reduced in immunoblot. Blue downward arrow = Downregulation; Red upward arrow = Upregulation.

Article Snippet: BIRC3 , 1:1000 , Cell Signaling Technology, Danvers, MA, USA , 3130.

Techniques: Membrane, Activation Assay, Activity Assay, Western Blot

Chidamide regulated the proteins associated with glycolysis, ferroptosis and DDP sensitivity by down-regulating USP35. A549 cells with treatment of 0.8 µM chidamide and H460 cells with treatment of 3.2 µM chidamide or not were transfected with oe-NC or oe-USP35. A - D WB analysis ( A ) was administrated for protein examination of PKM2 ( B ), FPN ( C ), and BIRC3 ( D ) in A549 cells. E - H WB results ( E ) for PKM2 ( F ), FPN ( G ), and BIRC3 ( H ) in H460 cells. */# P < 0.05, **/##/&& P < 0.01, ***/###/&&& P < 0.001. * : compared to oe-NC group, # : compared to oe-USP35 group, & : compared to chidamide + oe-NC group

Journal: BMC Cancer

Article Title: Chidamide impedes glycolysis but increases ferroptosis and cisplatin sensitivity of lung cancer cells through downregulating USP35

doi: 10.1186/s12885-025-14925-z

Figure Lengend Snippet: Chidamide regulated the proteins associated with glycolysis, ferroptosis and DDP sensitivity by down-regulating USP35. A549 cells with treatment of 0.8 µM chidamide and H460 cells with treatment of 3.2 µM chidamide or not were transfected with oe-NC or oe-USP35. A - D WB analysis ( A ) was administrated for protein examination of PKM2 ( B ), FPN ( C ), and BIRC3 ( D ) in A549 cells. E - H WB results ( E ) for PKM2 ( F ), FPN ( G ), and BIRC3 ( H ) in H460 cells. */# P < 0.05, **/##/&& P < 0.01, ***/###/&&& P < 0.001. * : compared to oe-NC group, # : compared to oe-USP35 group, & : compared to chidamide + oe-NC group

Article Snippet: The primary antibodies against USP35 (proteintech, Wuhan, China, 24559-1-AP), PKM2 (proteintech, 15822-1-AP), FPN (proteintech, 26601-1-AP), BIRC3 (proteintech, 24304-1-AP), β-actin (proteintech, 20536-1-AP) were incubated overnight at 4°C.

Techniques: Transfection

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