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bafa  (Tocris)


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    Structured Review

    Tocris bafa
    ( A-F ) MEFs were treated with vehicle control or 1µM PP for ∼24 hours (22 hours for E and F ) and then co-treated with or without puromycin (10 μg/mL for 30 minutes), MG132 (10μM for 110 minutes) or Baf A (100nM for 22 hours) (n=3 for all samples). Western blot and quantification of puromycin-incorporated proteins ( A, B ), poly-ubiquitinated protein ( C, D ) and LC3II and LC3II/LC3I ratio ( E, F ). ( G-H ) NIH3T3 RHO P23H cells were treated with 0.1µM PP for 24 hours and Western blot ( G ) and quantification ( H ) of RHO P23H level (n=3). ( I, J ) NIH3T3 RHO P23H cells were co-treated with or without MG132 (10μM for 120 minutes). Western blot ( I ) and quantification ( J ) of RHO P23H level (n=3). ( K ) qPCR of proteostasis and YAP1 target genes in P301S-expressing primary neurons treated with or without PP (0.1µM) for 12 hours. ( K-M ) Tau (P301S)-expressing primary neurons were treated with increasing concentration of PP for 24 hours, and western blot and quantification of different proteins (n=4 for HT7, PHF1 and LC3, and n=6 for P62) ( K ) or treated with 0.1µM PP for 12h hours in the presence or absence of <t>BafA</t> (50nM) in the last hour and western blot ( L ) and quantification (n=4 for total Tau and P62 and n=8 for p-Tau) ( M ) of different proteins. ( N ) Tau P301S-expressing neurons were treated with DMSO or 0.1µM PP for 12 hours and qPCR of selective proteostasis and YAP1 target genes (n=6∼11). ( O ) MEFs were treated with DMSO, PP <t>(1µM),</t> <t>XMU-MP-1</t> (1µM) or XMU-MP-1+PP for 24h hours, and qPCR of protein quality control and YAP1s output gene expression quantified as log transformed fold change under DMSO or XMU-MP-1 condition by PP (n=3). All data mean ± S.E.M.
    Bafa, supplied by Tocris, used in various techniques. Bioz Stars score: 95/100, based on 371 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bafa/product/Tocris
    Average 95 stars, based on 371 article reviews
    bafa - by Bioz Stars, 2026-03
    95/100 stars

    Images

    1) Product Images from "Nuclear speckle rejuvenation alleviates proteinopathies at the expense of YAP1"

    Article Title: Nuclear speckle rejuvenation alleviates proteinopathies at the expense of YAP1

    Journal: bioRxiv

    doi: 10.1101/2024.04.18.590103

    ( A-F ) MEFs were treated with vehicle control or 1µM PP for ∼24 hours (22 hours for E and F ) and then co-treated with or without puromycin (10 μg/mL for 30 minutes), MG132 (10μM for 110 minutes) or Baf A (100nM for 22 hours) (n=3 for all samples). Western blot and quantification of puromycin-incorporated proteins ( A, B ), poly-ubiquitinated protein ( C, D ) and LC3II and LC3II/LC3I ratio ( E, F ). ( G-H ) NIH3T3 RHO P23H cells were treated with 0.1µM PP for 24 hours and Western blot ( G ) and quantification ( H ) of RHO P23H level (n=3). ( I, J ) NIH3T3 RHO P23H cells were co-treated with or without MG132 (10μM for 120 minutes). Western blot ( I ) and quantification ( J ) of RHO P23H level (n=3). ( K ) qPCR of proteostasis and YAP1 target genes in P301S-expressing primary neurons treated with or without PP (0.1µM) for 12 hours. ( K-M ) Tau (P301S)-expressing primary neurons were treated with increasing concentration of PP for 24 hours, and western blot and quantification of different proteins (n=4 for HT7, PHF1 and LC3, and n=6 for P62) ( K ) or treated with 0.1µM PP for 12h hours in the presence or absence of BafA (50nM) in the last hour and western blot ( L ) and quantification (n=4 for total Tau and P62 and n=8 for p-Tau) ( M ) of different proteins. ( N ) Tau P301S-expressing neurons were treated with DMSO or 0.1µM PP for 12 hours and qPCR of selective proteostasis and YAP1 target genes (n=6∼11). ( O ) MEFs were treated with DMSO, PP (1µM), XMU-MP-1 (1µM) or XMU-MP-1+PP for 24h hours, and qPCR of protein quality control and YAP1s output gene expression quantified as log transformed fold change under DMSO or XMU-MP-1 condition by PP (n=3). All data mean ± S.E.M.
    Figure Legend Snippet: ( A-F ) MEFs were treated with vehicle control or 1µM PP for ∼24 hours (22 hours for E and F ) and then co-treated with or without puromycin (10 μg/mL for 30 minutes), MG132 (10μM for 110 minutes) or Baf A (100nM for 22 hours) (n=3 for all samples). Western blot and quantification of puromycin-incorporated proteins ( A, B ), poly-ubiquitinated protein ( C, D ) and LC3II and LC3II/LC3I ratio ( E, F ). ( G-H ) NIH3T3 RHO P23H cells were treated with 0.1µM PP for 24 hours and Western blot ( G ) and quantification ( H ) of RHO P23H level (n=3). ( I, J ) NIH3T3 RHO P23H cells were co-treated with or without MG132 (10μM for 120 minutes). Western blot ( I ) and quantification ( J ) of RHO P23H level (n=3). ( K ) qPCR of proteostasis and YAP1 target genes in P301S-expressing primary neurons treated with or without PP (0.1µM) for 12 hours. ( K-M ) Tau (P301S)-expressing primary neurons were treated with increasing concentration of PP for 24 hours, and western blot and quantification of different proteins (n=4 for HT7, PHF1 and LC3, and n=6 for P62) ( K ) or treated with 0.1µM PP for 12h hours in the presence or absence of BafA (50nM) in the last hour and western blot ( L ) and quantification (n=4 for total Tau and P62 and n=8 for p-Tau) ( M ) of different proteins. ( N ) Tau P301S-expressing neurons were treated with DMSO or 0.1µM PP for 12 hours and qPCR of selective proteostasis and YAP1 target genes (n=6∼11). ( O ) MEFs were treated with DMSO, PP (1µM), XMU-MP-1 (1µM) or XMU-MP-1+PP for 24h hours, and qPCR of protein quality control and YAP1s output gene expression quantified as log transformed fold change under DMSO or XMU-MP-1 condition by PP (n=3). All data mean ± S.E.M.

    Techniques Used: Control, Western Blot, Expressing, Concentration Assay, Transformation Assay



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    ( A-F ) MEFs were treated with vehicle control or 1µM PP for ∼24 hours (22 hours for E and F ) and then co-treated with or without puromycin (10 μg/mL for 30 minutes), MG132 (10μM for 110 minutes) or Baf A (100nM for 22 hours) (n=3 for all samples). Western blot and quantification of puromycin-incorporated proteins ( A, B ), poly-ubiquitinated protein ( C, D ) and LC3II and LC3II/LC3I ratio ( E, F ). ( G-H ) NIH3T3 RHO P23H cells were treated with 0.1µM PP for 24 hours and Western blot ( G ) and quantification ( H ) of RHO P23H level (n=3). ( I, J ) NIH3T3 RHO P23H cells were co-treated with or without MG132 (10μM for 120 minutes). Western blot ( I ) and quantification ( J ) of RHO P23H level (n=3). ( K ) qPCR of proteostasis and YAP1 target genes in P301S-expressing primary neurons treated with or without PP (0.1µM) for 12 hours. ( K-M ) Tau (P301S)-expressing primary neurons were treated with increasing concentration of PP for 24 hours, and western blot and quantification of different proteins (n=4 for HT7, PHF1 and LC3, and n=6 for P62) ( K ) or treated with 0.1µM PP for 12h hours in the presence or absence of <t>BafA</t> (50nM) in the last hour and western blot ( L ) and quantification (n=4 for total Tau and P62 and n=8 for p-Tau) ( M ) of different proteins. ( N ) Tau P301S-expressing neurons were treated with DMSO or 0.1µM PP for 12 hours and qPCR of selective proteostasis and YAP1 target genes (n=6∼11). ( O ) MEFs were treated with DMSO, PP <t>(1µM),</t> <t>XMU-MP-1</t> (1µM) or XMU-MP-1+PP for 24h hours, and qPCR of protein quality control and YAP1s output gene expression quantified as log transformed fold change under DMSO or XMU-MP-1 condition by PP (n=3). All data mean ± S.E.M.
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    ( A-F ) MEFs were treated with vehicle control or 1µM PP for ∼24 hours (22 hours for E and F ) and then co-treated with or without puromycin (10 μg/mL for 30 minutes), MG132 (10μM for 110 minutes) or Baf A (100nM for 22 hours) (n=3 for all samples). Western blot and quantification of puromycin-incorporated proteins ( A, B ), poly-ubiquitinated protein ( C, D ) and LC3II and LC3II/LC3I ratio ( E, F ). ( G-H ) NIH3T3 RHO P23H cells were treated with 0.1µM PP for 24 hours and Western blot ( G ) and quantification ( H ) of RHO P23H level (n=3). ( I, J ) NIH3T3 RHO P23H cells were co-treated with or without MG132 (10μM for 120 minutes). Western blot ( I ) and quantification ( J ) of RHO P23H level (n=3). ( K ) qPCR of proteostasis and YAP1 target genes in P301S-expressing primary neurons treated with or without PP (0.1µM) for 12 hours. ( K-M ) Tau (P301S)-expressing primary neurons were treated with increasing concentration of PP for 24 hours, and western blot and quantification of different proteins (n=4 for HT7, PHF1 and LC3, and n=6 for P62) ( K ) or treated with 0.1µM PP for 12h hours in the presence or absence of <t>BafA</t> (50nM) in the last hour and western blot ( L ) and quantification (n=4 for total Tau and P62 and n=8 for p-Tau) ( M ) of different proteins. ( N ) Tau P301S-expressing neurons were treated with DMSO or 0.1µM PP for 12 hours and qPCR of selective proteostasis and YAP1 target genes (n=6∼11). ( O ) MEFs were treated with DMSO, PP <t>(1µM),</t> <t>XMU-MP-1</t> (1µM) or XMU-MP-1+PP for 24h hours, and qPCR of protein quality control and YAP1s output gene expression quantified as log transformed fold change under DMSO or XMU-MP-1 condition by PP (n=3). All data mean ± S.E.M.
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    ( A-F ) MEFs were treated with vehicle control or 1µM PP for ∼24 hours (22 hours for E and F ) and then co-treated with or without puromycin (10 μg/mL for 30 minutes), MG132 (10μM for 110 minutes) or Baf A (100nM for 22 hours) (n=3 for all samples). Western blot and quantification of puromycin-incorporated proteins ( A, B ), poly-ubiquitinated protein ( C, D ) and LC3II and LC3II/LC3I ratio ( E, F ). ( G-H ) NIH3T3 RHO P23H cells were treated with 0.1µM PP for 24 hours and Western blot ( G ) and quantification ( H ) of RHO P23H level (n=3). ( I, J ) NIH3T3 RHO P23H cells were co-treated with or without MG132 (10μM for 120 minutes). Western blot ( I ) and quantification ( J ) of RHO P23H level (n=3). ( K ) qPCR of proteostasis and YAP1 target genes in P301S-expressing primary neurons treated with or without PP (0.1µM) for 12 hours. ( K-M ) Tau (P301S)-expressing primary neurons were treated with increasing concentration of PP for 24 hours, and western blot and quantification of different proteins (n=4 for HT7, PHF1 and LC3, and n=6 for P62) ( K ) or treated with 0.1µM PP for 12h hours in the presence or absence of <t>BafA</t> (50nM) in the last hour and western blot ( L ) and quantification (n=4 for total Tau and P62 and n=8 for p-Tau) ( M ) of different proteins. ( N ) Tau P301S-expressing neurons were treated with DMSO or 0.1µM PP for 12 hours and qPCR of selective proteostasis and YAP1 target genes (n=6∼11). ( O ) MEFs were treated with DMSO, PP <t>(1µM),</t> <t>XMU-MP-1</t> (1µM) or XMU-MP-1+PP for 24h hours, and qPCR of protein quality control and YAP1s output gene expression quantified as log transformed fold change under DMSO or XMU-MP-1 condition by PP (n=3). All data mean ± S.E.M.
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    ( A-F ) MEFs were treated with vehicle control or 1µM PP for ∼24 hours (22 hours for E and F ) and then co-treated with or without puromycin (10 μg/mL for 30 minutes), MG132 (10μM for 110 minutes) or Baf A (100nM for 22 hours) (n=3 for all samples). Western blot and quantification of puromycin-incorporated proteins ( A, B ), poly-ubiquitinated protein ( C, D ) and LC3II and LC3II/LC3I ratio ( E, F ). ( G-H ) NIH3T3 RHO P23H cells were treated with 0.1µM PP for 24 hours and Western blot ( G ) and quantification ( H ) of RHO P23H level (n=3). ( I, J ) NIH3T3 RHO P23H cells were co-treated with or without MG132 (10μM for 120 minutes). Western blot ( I ) and quantification ( J ) of RHO P23H level (n=3). ( K ) qPCR of proteostasis and YAP1 target genes in P301S-expressing primary neurons treated with or without PP (0.1µM) for 12 hours. ( K-M ) Tau (P301S)-expressing primary neurons were treated with increasing concentration of PP for 24 hours, and western blot and quantification of different proteins (n=4 for HT7, PHF1 and LC3, and n=6 for P62) ( K ) or treated with 0.1µM PP for 12h hours in the presence or absence of <t>BafA</t> (50nM) in the last hour and western blot ( L ) and quantification (n=4 for total Tau and P62 and n=8 for p-Tau) ( M ) of different proteins. ( N ) Tau P301S-expressing neurons were treated with DMSO or 0.1µM PP for 12 hours and qPCR of selective proteostasis and YAP1 target genes (n=6∼11). ( O ) MEFs were treated with DMSO, PP <t>(1µM),</t> <t>XMU-MP-1</t> (1µM) or XMU-MP-1+PP for 24h hours, and qPCR of protein quality control and YAP1s output gene expression quantified as log transformed fold change under DMSO or XMU-MP-1 condition by PP (n=3). All data mean ± S.E.M.
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    ( A-F ) MEFs were treated with vehicle control or 1µM PP for ∼24 hours (22 hours for E and F ) and then co-treated with or without puromycin (10 μg/mL for 30 minutes), MG132 (10μM for 110 minutes) or Baf A (100nM for 22 hours) (n=3 for all samples). Western blot and quantification of puromycin-incorporated proteins ( A, B ), poly-ubiquitinated protein ( C, D ) and LC3II and LC3II/LC3I ratio ( E, F ). ( G-H ) NIH3T3 RHO P23H cells were treated with 0.1µM PP for 24 hours and Western blot ( G ) and quantification ( H ) of RHO P23H level (n=3). ( I, J ) NIH3T3 RHO P23H cells were co-treated with or without MG132 (10μM for 120 minutes). Western blot ( I ) and quantification ( J ) of RHO P23H level (n=3). ( K ) qPCR of proteostasis and YAP1 target genes in P301S-expressing primary neurons treated with or without PP (0.1µM) for 12 hours. ( K-M ) Tau (P301S)-expressing primary neurons were treated with increasing concentration of PP for 24 hours, and western blot and quantification of different proteins (n=4 for HT7, PHF1 and LC3, and n=6 for P62) ( K ) or treated with 0.1µM PP for 12h hours in the presence or absence of <t>BafA</t> (50nM) in the last hour and western blot ( L ) and quantification (n=4 for total Tau and P62 and n=8 for p-Tau) ( M ) of different proteins. ( N ) Tau P301S-expressing neurons were treated with DMSO or 0.1µM PP for 12 hours and qPCR of selective proteostasis and YAP1 target genes (n=6∼11). ( O ) MEFs were treated with DMSO, PP <t>(1µM),</t> <t>XMU-MP-1</t> (1µM) or XMU-MP-1+PP for 24h hours, and qPCR of protein quality control and YAP1s output gene expression quantified as log transformed fold change under DMSO or XMU-MP-1 condition by PP (n=3). All data mean ± S.E.M.
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    Image Search Results


    ( A-F ) MEFs were treated with vehicle control or 1µM PP for ∼24 hours (22 hours for E and F ) and then co-treated with or without puromycin (10 μg/mL for 30 minutes), MG132 (10μM for 110 minutes) or Baf A (100nM for 22 hours) (n=3 for all samples). Western blot and quantification of puromycin-incorporated proteins ( A, B ), poly-ubiquitinated protein ( C, D ) and LC3II and LC3II/LC3I ratio ( E, F ). ( G-H ) NIH3T3 RHO P23H cells were treated with 0.1µM PP for 24 hours and Western blot ( G ) and quantification ( H ) of RHO P23H level (n=3). ( I, J ) NIH3T3 RHO P23H cells were co-treated with or without MG132 (10μM for 120 minutes). Western blot ( I ) and quantification ( J ) of RHO P23H level (n=3). ( K ) qPCR of proteostasis and YAP1 target genes in P301S-expressing primary neurons treated with or without PP (0.1µM) for 12 hours. ( K-M ) Tau (P301S)-expressing primary neurons were treated with increasing concentration of PP for 24 hours, and western blot and quantification of different proteins (n=4 for HT7, PHF1 and LC3, and n=6 for P62) ( K ) or treated with 0.1µM PP for 12h hours in the presence or absence of BafA (50nM) in the last hour and western blot ( L ) and quantification (n=4 for total Tau and P62 and n=8 for p-Tau) ( M ) of different proteins. ( N ) Tau P301S-expressing neurons were treated with DMSO or 0.1µM PP for 12 hours and qPCR of selective proteostasis and YAP1 target genes (n=6∼11). ( O ) MEFs were treated with DMSO, PP (1µM), XMU-MP-1 (1µM) or XMU-MP-1+PP for 24h hours, and qPCR of protein quality control and YAP1s output gene expression quantified as log transformed fold change under DMSO or XMU-MP-1 condition by PP (n=3). All data mean ± S.E.M.

    Journal: bioRxiv

    Article Title: Nuclear speckle rejuvenation alleviates proteinopathies at the expense of YAP1

    doi: 10.1101/2024.04.18.590103

    Figure Lengend Snippet: ( A-F ) MEFs were treated with vehicle control or 1µM PP for ∼24 hours (22 hours for E and F ) and then co-treated with or without puromycin (10 μg/mL for 30 minutes), MG132 (10μM for 110 minutes) or Baf A (100nM for 22 hours) (n=3 for all samples). Western blot and quantification of puromycin-incorporated proteins ( A, B ), poly-ubiquitinated protein ( C, D ) and LC3II and LC3II/LC3I ratio ( E, F ). ( G-H ) NIH3T3 RHO P23H cells were treated with 0.1µM PP for 24 hours and Western blot ( G ) and quantification ( H ) of RHO P23H level (n=3). ( I, J ) NIH3T3 RHO P23H cells were co-treated with or without MG132 (10μM for 120 minutes). Western blot ( I ) and quantification ( J ) of RHO P23H level (n=3). ( K ) qPCR of proteostasis and YAP1 target genes in P301S-expressing primary neurons treated with or without PP (0.1µM) for 12 hours. ( K-M ) Tau (P301S)-expressing primary neurons were treated with increasing concentration of PP for 24 hours, and western blot and quantification of different proteins (n=4 for HT7, PHF1 and LC3, and n=6 for P62) ( K ) or treated with 0.1µM PP for 12h hours in the presence or absence of BafA (50nM) in the last hour and western blot ( L ) and quantification (n=4 for total Tau and P62 and n=8 for p-Tau) ( M ) of different proteins. ( N ) Tau P301S-expressing neurons were treated with DMSO or 0.1µM PP for 12 hours and qPCR of selective proteostasis and YAP1 target genes (n=6∼11). ( O ) MEFs were treated with DMSO, PP (1µM), XMU-MP-1 (1µM) or XMU-MP-1+PP for 24h hours, and qPCR of protein quality control and YAP1s output gene expression quantified as log transformed fold change under DMSO or XMU-MP-1 condition by PP (n=3). All data mean ± S.E.M.

    Article Snippet: NB (HY-50904), PB (HY-12047), PH (HY-B0883), PP (HY-A0293), MG-132 (HY-13259), and XMU-MP-1 (HY-100526) were purchased through MedChemExpress and BafA (1334) was purchased from Tocris.

    Techniques: Control, Western Blot, Expressing, Concentration Assay, Transformation Assay