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bafa 1  (MedChemExpress)
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MedChemExpress bafa 1
Bafa 1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bafa  (Tocris)
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( A-F ) MEFs were treated with vehicle control or 1µM PP for ∼24 hours (22 hours for E and F ) and then co-treated with or without puromycin (10 μg/mL for 30 minutes), MG132 (10μM for 110 minutes) or Baf A (100nM for 22 hours) (n=3 for all samples). Western blot and quantification of puromycin-incorporated proteins ( A, B ), poly-ubiquitinated protein ( C, D ) and LC3II and LC3II/LC3I ratio ( E, F ). ( G-H ) NIH3T3 RHO P23H cells were treated with 0.1µM PP for 24 hours and Western blot ( G ) and quantification ( H ) of RHO P23H level (n=3). ( I, J ) NIH3T3 RHO P23H cells were co-treated with or without MG132 (10μM for 120 minutes). Western blot ( I ) and quantification ( J ) of RHO P23H level (n=3). ( K ) qPCR of proteostasis and YAP1 target genes in P301S-expressing primary neurons treated with or without PP (0.1µM) for 12 hours. ( K-M ) Tau (P301S)-expressing primary neurons were treated with increasing concentration of PP for 24 hours, and western blot and quantification of different proteins (n=4 for HT7, PHF1 and LC3, and n=6 for P62) ( K ) or treated with 0.1µM PP for 12h hours in the presence or absence of <t>BafA</t> (50nM) in the last hour and western blot ( L ) and quantification (n=4 for total Tau and P62 and n=8 for p-Tau) ( M ) of different proteins. ( N ) Tau P301S-expressing neurons were treated with DMSO or 0.1µM PP for 12 hours and qPCR of selective proteostasis and YAP1 target genes (n=6∼11). ( O ) MEFs were treated with DMSO, PP <t>(1µM),</t> <t>XMU-MP-1</t> (1µM) or XMU-MP-1+PP for 24h hours, and qPCR of protein quality control and YAP1s output gene expression quantified as log transformed fold change under DMSO or XMU-MP-1 condition by PP (n=3). All data mean ± S.E.M.
Bafa, supplied by Tocris, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bafa  (LC Laboratories)
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( A-F ) MEFs were treated with vehicle control or 1µM PP for ∼24 hours (22 hours for E and F ) and then co-treated with or without puromycin (10 μg/mL for 30 minutes), MG132 (10μM for 110 minutes) or Baf A (100nM for 22 hours) (n=3 for all samples). Western blot and quantification of puromycin-incorporated proteins ( A, B ), poly-ubiquitinated protein ( C, D ) and LC3II and LC3II/LC3I ratio ( E, F ). ( G-H ) NIH3T3 RHO P23H cells were treated with 0.1µM PP for 24 hours and Western blot ( G ) and quantification ( H ) of RHO P23H level (n=3). ( I, J ) NIH3T3 RHO P23H cells were co-treated with or without MG132 (10μM for 120 minutes). Western blot ( I ) and quantification ( J ) of RHO P23H level (n=3). ( K ) qPCR of proteostasis and YAP1 target genes in P301S-expressing primary neurons treated with or without PP (0.1µM) for 12 hours. ( K-M ) Tau (P301S)-expressing primary neurons were treated with increasing concentration of PP for 24 hours, and western blot and quantification of different proteins (n=4 for HT7, PHF1 and LC3, and n=6 for P62) ( K ) or treated with 0.1µM PP for 12h hours in the presence or absence of <t>BafA</t> (50nM) in the last hour and western blot ( L ) and quantification (n=4 for total Tau and P62 and n=8 for p-Tau) ( M ) of different proteins. ( N ) Tau P301S-expressing neurons were treated with DMSO or 0.1µM PP for 12 hours and qPCR of selective proteostasis and YAP1 target genes (n=6∼11). ( O ) MEFs were treated with DMSO, PP <t>(1µM),</t> <t>XMU-MP-1</t> (1µM) or XMU-MP-1+PP for 24h hours, and qPCR of protein quality control and YAP1s output gene expression quantified as log transformed fold change under DMSO or XMU-MP-1 condition by PP (n=3). All data mean ± S.E.M.
Bafa, supplied by LC Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bafa 2,2-bis(3-amino-4-hydroxyphenyl)hexafluoropropane  (Nippon Kayaku)
90
Nippon Kayaku bafa 2,2-bis(3-amino-4-hydroxyphenyl)hexafluoropropane
( A-F ) MEFs were treated with vehicle control or 1µM PP for ∼24 hours (22 hours for E and F ) and then co-treated with or without puromycin (10 μg/mL for 30 minutes), MG132 (10μM for 110 minutes) or Baf A (100nM for 22 hours) (n=3 for all samples). Western blot and quantification of puromycin-incorporated proteins ( A, B ), poly-ubiquitinated protein ( C, D ) and LC3II and LC3II/LC3I ratio ( E, F ). ( G-H ) NIH3T3 RHO P23H cells were treated with 0.1µM PP for 24 hours and Western blot ( G ) and quantification ( H ) of RHO P23H level (n=3). ( I, J ) NIH3T3 RHO P23H cells were co-treated with or without MG132 (10μM for 120 minutes). Western blot ( I ) and quantification ( J ) of RHO P23H level (n=3). ( K ) qPCR of proteostasis and YAP1 target genes in P301S-expressing primary neurons treated with or without PP (0.1µM) for 12 hours. ( K-M ) Tau (P301S)-expressing primary neurons were treated with increasing concentration of PP for 24 hours, and western blot and quantification of different proteins (n=4 for HT7, PHF1 and LC3, and n=6 for P62) ( K ) or treated with 0.1µM PP for 12h hours in the presence or absence of <t>BafA</t> (50nM) in the last hour and western blot ( L ) and quantification (n=4 for total Tau and P62 and n=8 for p-Tau) ( M ) of different proteins. ( N ) Tau P301S-expressing neurons were treated with DMSO or 0.1µM PP for 12 hours and qPCR of selective proteostasis and YAP1 target genes (n=6∼11). ( O ) MEFs were treated with DMSO, PP <t>(1µM),</t> <t>XMU-MP-1</t> (1µM) or XMU-MP-1+PP for 24h hours, and qPCR of protein quality control and YAP1s output gene expression quantified as log transformed fold change under DMSO or XMU-MP-1 condition by PP (n=3). All data mean ± S.E.M.
Bafa 2,2 Bis(3 Amino 4 Hydroxyphenyl)Hexafluoropropane, supplied by Nippon Kayaku, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bafa 1 treatment  (MedChemExpress)
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( A-F ) MEFs were treated with vehicle control or 1µM PP for ∼24 hours (22 hours for E and F ) and then co-treated with or without puromycin (10 μg/mL for 30 minutes), MG132 (10μM for 110 minutes) or Baf A (100nM for 22 hours) (n=3 for all samples). Western blot and quantification of puromycin-incorporated proteins ( A, B ), poly-ubiquitinated protein ( C, D ) and LC3II and LC3II/LC3I ratio ( E, F ). ( G-H ) NIH3T3 RHO P23H cells were treated with 0.1µM PP for 24 hours and Western blot ( G ) and quantification ( H ) of RHO P23H level (n=3). ( I, J ) NIH3T3 RHO P23H cells were co-treated with or without MG132 (10μM for 120 minutes). Western blot ( I ) and quantification ( J ) of RHO P23H level (n=3). ( K ) qPCR of proteostasis and YAP1 target genes in P301S-expressing primary neurons treated with or without PP (0.1µM) for 12 hours. ( K-M ) Tau (P301S)-expressing primary neurons were treated with increasing concentration of PP for 24 hours, and western blot and quantification of different proteins (n=4 for HT7, PHF1 and LC3, and n=6 for P62) ( K ) or treated with 0.1µM PP for 12h hours in the presence or absence of <t>BafA</t> (50nM) in the last hour and western blot ( L ) and quantification (n=4 for total Tau and P62 and n=8 for p-Tau) ( M ) of different proteins. ( N ) Tau P301S-expressing neurons were treated with DMSO or 0.1µM PP for 12 hours and qPCR of selective proteostasis and YAP1 target genes (n=6∼11). ( O ) MEFs were treated with DMSO, PP <t>(1µM),</t> <t>XMU-MP-1</t> (1µM) or XMU-MP-1+PP for 24h hours, and qPCR of protein quality control and YAP1s output gene expression quantified as log transformed fold change under DMSO or XMU-MP-1 condition by PP (n=3). All data mean ± S.E.M.
Bafa 1 Treatment, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bafilomycin a1  (MedChemExpress)
96
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( A-F ) MEFs were treated with vehicle control or 1µM PP for ∼24 hours (22 hours for E and F ) and then co-treated with or without puromycin (10 μg/mL for 30 minutes), MG132 (10μM for 110 minutes) or Baf A (100nM for 22 hours) (n=3 for all samples). Western blot and quantification of puromycin-incorporated proteins ( A, B ), poly-ubiquitinated protein ( C, D ) and LC3II and LC3II/LC3I ratio ( E, F ). ( G-H ) NIH3T3 RHO P23H cells were treated with 0.1µM PP for 24 hours and Western blot ( G ) and quantification ( H ) of RHO P23H level (n=3). ( I, J ) NIH3T3 RHO P23H cells were co-treated with or without MG132 (10μM for 120 minutes). Western blot ( I ) and quantification ( J ) of RHO P23H level (n=3). ( K ) qPCR of proteostasis and YAP1 target genes in P301S-expressing primary neurons treated with or without PP (0.1µM) for 12 hours. ( K-M ) Tau (P301S)-expressing primary neurons were treated with increasing concentration of PP for 24 hours, and western blot and quantification of different proteins (n=4 for HT7, PHF1 and LC3, and n=6 for P62) ( K ) or treated with 0.1µM PP for 12h hours in the presence or absence of <t>BafA</t> (50nM) in the last hour and western blot ( L ) and quantification (n=4 for total Tau and P62 and n=8 for p-Tau) ( M ) of different proteins. ( N ) Tau P301S-expressing neurons were treated with DMSO or 0.1µM PP for 12 hours and qPCR of selective proteostasis and YAP1 target genes (n=6∼11). ( O ) MEFs were treated with DMSO, PP <t>(1µM),</t> <t>XMU-MP-1</t> (1µM) or XMU-MP-1+PP for 24h hours, and qPCR of protein quality control and YAP1s output gene expression quantified as log transformed fold change under DMSO or XMU-MP-1 condition by PP (n=3). All data mean ± S.E.M.
Bafilomycin A1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bafilomycin a1 bafa  (MedChemExpress)
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( A-F ) MEFs were treated with vehicle control or 1µM PP for ∼24 hours (22 hours for E and F ) and then co-treated with or without puromycin (10 μg/mL for 30 minutes), MG132 (10μM for 110 minutes) or Baf A (100nM for 22 hours) (n=3 for all samples). Western blot and quantification of puromycin-incorporated proteins ( A, B ), poly-ubiquitinated protein ( C, D ) and LC3II and LC3II/LC3I ratio ( E, F ). ( G-H ) NIH3T3 RHO P23H cells were treated with 0.1µM PP for 24 hours and Western blot ( G ) and quantification ( H ) of RHO P23H level (n=3). ( I, J ) NIH3T3 RHO P23H cells were co-treated with or without MG132 (10μM for 120 minutes). Western blot ( I ) and quantification ( J ) of RHO P23H level (n=3). ( K ) qPCR of proteostasis and YAP1 target genes in P301S-expressing primary neurons treated with or without PP (0.1µM) for 12 hours. ( K-M ) Tau (P301S)-expressing primary neurons were treated with increasing concentration of PP for 24 hours, and western blot and quantification of different proteins (n=4 for HT7, PHF1 and LC3, and n=6 for P62) ( K ) or treated with 0.1µM PP for 12h hours in the presence or absence of <t>BafA</t> (50nM) in the last hour and western blot ( L ) and quantification (n=4 for total Tau and P62 and n=8 for p-Tau) ( M ) of different proteins. ( N ) Tau P301S-expressing neurons were treated with DMSO or 0.1µM PP for 12 hours and qPCR of selective proteostasis and YAP1 target genes (n=6∼11). ( O ) MEFs were treated with DMSO, PP <t>(1µM),</t> <t>XMU-MP-1</t> (1µM) or XMU-MP-1+PP for 24h hours, and qPCR of protein quality control and YAP1s output gene expression quantified as log transformed fold change under DMSO or XMU-MP-1 condition by PP (n=3). All data mean ± S.E.M.
Bafilomycin A1 Bafa, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bafa 1  (Millipore)
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( A-F ) MEFs were treated with vehicle control or 1µM PP for ∼24 hours (22 hours for E and F ) and then co-treated with or without puromycin (10 μg/mL for 30 minutes), MG132 (10μM for 110 minutes) or Baf A (100nM for 22 hours) (n=3 for all samples). Western blot and quantification of puromycin-incorporated proteins ( A, B ), poly-ubiquitinated protein ( C, D ) and LC3II and LC3II/LC3I ratio ( E, F ). ( G-H ) NIH3T3 RHO P23H cells were treated with 0.1µM PP for 24 hours and Western blot ( G ) and quantification ( H ) of RHO P23H level (n=3). ( I, J ) NIH3T3 RHO P23H cells were co-treated with or without MG132 (10μM for 120 minutes). Western blot ( I ) and quantification ( J ) of RHO P23H level (n=3). ( K ) qPCR of proteostasis and YAP1 target genes in P301S-expressing primary neurons treated with or without PP (0.1µM) for 12 hours. ( K-M ) Tau (P301S)-expressing primary neurons were treated with increasing concentration of PP for 24 hours, and western blot and quantification of different proteins (n=4 for HT7, PHF1 and LC3, and n=6 for P62) ( K ) or treated with 0.1µM PP for 12h hours in the presence or absence of <t>BafA</t> (50nM) in the last hour and western blot ( L ) and quantification (n=4 for total Tau and P62 and n=8 for p-Tau) ( M ) of different proteins. ( N ) Tau P301S-expressing neurons were treated with DMSO or 0.1µM PP for 12 hours and qPCR of selective proteostasis and YAP1 target genes (n=6∼11). ( O ) MEFs were treated with DMSO, PP <t>(1µM),</t> <t>XMU-MP-1</t> (1µM) or XMU-MP-1+PP for 24h hours, and qPCR of protein quality control and YAP1s output gene expression quantified as log transformed fold change under DMSO or XMU-MP-1 condition by PP (n=3). All data mean ± S.E.M.
Bafa 1, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( A-F ) MEFs were treated with vehicle control or 1µM PP for ∼24 hours (22 hours for E and F ) and then co-treated with or without puromycin (10 μg/mL for 30 minutes), MG132 (10μM for 110 minutes) or Baf A (100nM for 22 hours) (n=3 for all samples). Western blot and quantification of puromycin-incorporated proteins ( A, B ), poly-ubiquitinated protein ( C, D ) and LC3II and LC3II/LC3I ratio ( E, F ). ( G-H ) NIH3T3 RHO P23H cells were treated with 0.1µM PP for 24 hours and Western blot ( G ) and quantification ( H ) of RHO P23H level (n=3). ( I, J ) NIH3T3 RHO P23H cells were co-treated with or without MG132 (10μM for 120 minutes). Western blot ( I ) and quantification ( J ) of RHO P23H level (n=3). ( K ) qPCR of proteostasis and YAP1 target genes in P301S-expressing primary neurons treated with or without PP (0.1µM) for 12 hours. ( K-M ) Tau (P301S)-expressing primary neurons were treated with increasing concentration of PP for 24 hours, and western blot and quantification of different proteins (n=4 for HT7, PHF1 and LC3, and n=6 for P62) ( K ) or treated with 0.1µM PP for 12h hours in the presence or absence of BafA (50nM) in the last hour and western blot ( L ) and quantification (n=4 for total Tau and P62 and n=8 for p-Tau) ( M ) of different proteins. ( N ) Tau P301S-expressing neurons were treated with DMSO or 0.1µM PP for 12 hours and qPCR of selective proteostasis and YAP1 target genes (n=6∼11). ( O ) MEFs were treated with DMSO, PP (1µM), XMU-MP-1 (1µM) or XMU-MP-1+PP for 24h hours, and qPCR of protein quality control and YAP1s output gene expression quantified as log transformed fold change under DMSO or XMU-MP-1 condition by PP (n=3). All data mean ± S.E.M.

Journal: bioRxiv

Article Title: Nuclear speckle rejuvenation alleviates proteinopathies at the expense of YAP1

doi: 10.1101/2024.04.18.590103

Figure Lengend Snippet: ( A-F ) MEFs were treated with vehicle control or 1µM PP for ∼24 hours (22 hours for E and F ) and then co-treated with or without puromycin (10 μg/mL for 30 minutes), MG132 (10μM for 110 minutes) or Baf A (100nM for 22 hours) (n=3 for all samples). Western blot and quantification of puromycin-incorporated proteins ( A, B ), poly-ubiquitinated protein ( C, D ) and LC3II and LC3II/LC3I ratio ( E, F ). ( G-H ) NIH3T3 RHO P23H cells were treated with 0.1µM PP for 24 hours and Western blot ( G ) and quantification ( H ) of RHO P23H level (n=3). ( I, J ) NIH3T3 RHO P23H cells were co-treated with or without MG132 (10μM for 120 minutes). Western blot ( I ) and quantification ( J ) of RHO P23H level (n=3). ( K ) qPCR of proteostasis and YAP1 target genes in P301S-expressing primary neurons treated with or without PP (0.1µM) for 12 hours. ( K-M ) Tau (P301S)-expressing primary neurons were treated with increasing concentration of PP for 24 hours, and western blot and quantification of different proteins (n=4 for HT7, PHF1 and LC3, and n=6 for P62) ( K ) or treated with 0.1µM PP for 12h hours in the presence or absence of BafA (50nM) in the last hour and western blot ( L ) and quantification (n=4 for total Tau and P62 and n=8 for p-Tau) ( M ) of different proteins. ( N ) Tau P301S-expressing neurons were treated with DMSO or 0.1µM PP for 12 hours and qPCR of selective proteostasis and YAP1 target genes (n=6∼11). ( O ) MEFs were treated with DMSO, PP (1µM), XMU-MP-1 (1µM) or XMU-MP-1+PP for 24h hours, and qPCR of protein quality control and YAP1s output gene expression quantified as log transformed fold change under DMSO or XMU-MP-1 condition by PP (n=3). All data mean ± S.E.M.

Article Snippet: NB (HY-50904), PB (HY-12047), PH (HY-B0883), PP (HY-A0293), MG-132 (HY-13259), and XMU-MP-1 (HY-100526) were purchased through MedChemExpress and BafA (1334) was purchased from Tocris.

Techniques: Control, Western Blot, Expressing, Concentration Assay, Transformation Assay