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Survival in B16-F10 mouse model with antibiotic-gene free NanoP (A) Transfection of B16-F10 cells using 600 ng of <t>pUNO1</t> or nanoplasmid (NanoP) backbones as determined by flow cytometry. (B) GFP expression, determined by flow cytometry. Each data bar represents mean ± SEM with four biological replicates. (C) Average tumor growth of tumors treated with nanoplasmid 4T12 NPs + anti-PD1 vs. nanoplasmid luciferase NPs + anti-PD1. Each data curve represents eight biological replicates. Significance is represented by ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, and ∗∗∗∗ p ≤ 0.0001.
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Survival in B16-F10 mouse model with antibiotic-gene free NanoP (A) Transfection of B16-F10 cells using 600 ng of <t>pUNO1</t> or nanoplasmid (NanoP) backbones as determined by flow cytometry. (B) GFP expression, determined by flow cytometry. Each data bar represents mean ± SEM with four biological replicates. (C) Average tumor growth of tumors treated with nanoplasmid 4T12 NPs + anti-PD1 vs. nanoplasmid luciferase NPs + anti-PD1. Each data curve represents eight biological replicates. Significance is represented by ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, and ∗∗∗∗ p ≤ 0.0001.
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Survival in B16-F10 mouse model with antibiotic-gene free NanoP (A) Transfection of B16-F10 cells using 600 ng of <t>pUNO1</t> or nanoplasmid (NanoP) backbones as determined by flow cytometry. (B) GFP expression, determined by flow cytometry. Each data bar represents mean ± SEM with four biological replicates. (C) Average tumor growth of tumors treated with nanoplasmid 4T12 NPs + anti-PD1 vs. nanoplasmid luciferase NPs + anti-PD1. Each data curve represents eight biological replicates. Significance is represented by ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, and ∗∗∗∗ p ≤ 0.0001.
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Survival in B16-F10 mouse model with antibiotic-gene free NanoP (A) Transfection of B16-F10 cells using 600 ng of <t>pUNO1</t> or nanoplasmid (NanoP) backbones as determined by flow cytometry. (B) GFP expression, determined by flow cytometry. Each data bar represents mean ± SEM with four biological replicates. (C) Average tumor growth of tumors treated with nanoplasmid 4T12 NPs + anti-PD1 vs. nanoplasmid luciferase NPs + anti-PD1. Each data curve represents eight biological replicates. Significance is represented by ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, and ∗∗∗∗ p ≤ 0.0001.
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Survival in B16-F10 mouse model with antibiotic-gene free NanoP (A) Transfection of B16-F10 cells using 600 ng of <t>pUNO1</t> or nanoplasmid (NanoP) backbones as determined by flow cytometry. (B) GFP expression, determined by flow cytometry. Each data bar represents mean ± SEM with four biological replicates. (C) Average tumor growth of tumors treated with nanoplasmid 4T12 NPs + anti-PD1 vs. nanoplasmid luciferase NPs + anti-PD1. Each data curve represents eight biological replicates. Significance is represented by ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, and ∗∗∗∗ p ≤ 0.0001.
Single Stranded Aav Backbone V445 Pfb Gfp, supplied by Virovek Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Survival in B16-F10 mouse model with antibiotic-gene free NanoP (A) Transfection of B16-F10 cells using 600 ng of <t>pUNO1</t> or nanoplasmid (NanoP) backbones as determined by flow cytometry. (B) GFP expression, determined by flow cytometry. Each data bar represents mean ± SEM with four biological replicates. (C) Average tumor growth of tumors treated with nanoplasmid 4T12 NPs + anti-PD1 vs. nanoplasmid luciferase NPs + anti-PD1. Each data curve represents eight biological replicates. Significance is represented by ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, and ∗∗∗∗ p ≤ 0.0001.
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Survival in B16-F10 mouse model with antibiotic-gene free NanoP (A) Transfection of B16-F10 cells using 600 ng of <t>pUNO1</t> or nanoplasmid (NanoP) backbones as determined by flow cytometry. (B) GFP expression, determined by flow cytometry. Each data bar represents mean ± SEM with four biological replicates. (C) Average tumor growth of tumors treated with nanoplasmid 4T12 NPs + anti-PD1 vs. nanoplasmid luciferase NPs + anti-PD1. Each data curve represents eight biological replicates. Significance is represented by ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, and ∗∗∗∗ p ≤ 0.0001.
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Survival in B16-F10 mouse model with antibiotic-gene free NanoP (A) Transfection of B16-F10 cells using 600 ng of <t>pUNO1</t> or nanoplasmid (NanoP) backbones as determined by flow cytometry. (B) GFP expression, determined by flow cytometry. Each data bar represents mean ± SEM with four biological replicates. (C) Average tumor growth of tumors treated with nanoplasmid 4T12 NPs + anti-PD1 vs. nanoplasmid luciferase NPs + anti-PD1. Each data curve represents eight biological replicates. Significance is represented by ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, and ∗∗∗∗ p ≤ 0.0001.
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Creative Biogene Inc lentiviral backbone
Survival in B16-F10 mouse model with antibiotic-gene free NanoP (A) Transfection of B16-F10 cells using 600 ng of <t>pUNO1</t> or nanoplasmid (NanoP) backbones as determined by flow cytometry. (B) GFP expression, determined by flow cytometry. Each data bar represents mean ± SEM with four biological replicates. (C) Average tumor growth of tumors treated with nanoplasmid 4T12 NPs + anti-PD1 vs. nanoplasmid luciferase NPs + anti-PD1. Each data curve represents eight biological replicates. Significance is represented by ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, and ∗∗∗∗ p ≤ 0.0001.
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Image Search Results


Survival in B16-F10 mouse model with antibiotic-gene free NanoP (A) Transfection of B16-F10 cells using 600 ng of pUNO1 or nanoplasmid (NanoP) backbones as determined by flow cytometry. (B) GFP expression, determined by flow cytometry. Each data bar represents mean ± SEM with four biological replicates. (C) Average tumor growth of tumors treated with nanoplasmid 4T12 NPs + anti-PD1 vs. nanoplasmid luciferase NPs + anti-PD1. Each data curve represents eight biological replicates. Significance is represented by ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, and ∗∗∗∗ p ≤ 0.0001.

Journal: Molecular Therapy Advances

Article Title: Development of bicistronic plasmids and fusion proteins for clinical translation of tumor immune reprogramming

doi: 10.1016/j.omta.2026.201708

Figure Lengend Snippet: Survival in B16-F10 mouse model with antibiotic-gene free NanoP (A) Transfection of B16-F10 cells using 600 ng of pUNO1 or nanoplasmid (NanoP) backbones as determined by flow cytometry. (B) GFP expression, determined by flow cytometry. Each data bar represents mean ± SEM with four biological replicates. (C) Average tumor growth of tumors treated with nanoplasmid 4T12 NPs + anti-PD1 vs. nanoplasmid luciferase NPs + anti-PD1. Each data curve represents eight biological replicates. Significance is represented by ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, and ∗∗∗∗ p ≤ 0.0001.

Article Snippet: In tandem, the pUNO1 backbone from the plasmid pUNO1 MCS (InvivoGen, catalog no. pUNO1-mcs) was digested with NheI and SalI.

Techniques: Transfection, Flow Cytometry, Expressing, Luciferase