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baa-473  (Novartis)

 
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    Novartis baa-473
    Baa 473, supplied by Novartis, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/baa-473/product/Novartis
    Average 90 stars, based on 1 article reviews
    baa-473 - by Bioz Stars, 2026-03
    90/100 stars

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    Real-time PCR amplification conditions, efficiencies, calibration standard, and primers

    Journal: Applied and Environmental Microbiology

    Article Title: Short-Term Nitrogen Fertilization Affects Microbial Community Composition and Nitrogen Mineralization Functions in an Agricultural Soil

    doi: 10.1128/AEM.02278-19

    Figure Lengend Snippet: Real-time PCR amplification conditions, efficiencies, calibration standard, and primers

    Article Snippet: Duplicate assays for each gene and calibration standard series were measured in a single run. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Target gene Primer concn (μM) Size (bp) No. of cycles Length of PCR step (s) Efficiency (%) Calibration standard Primers (reference) Denaturation at 95°C Annealing (°C) Elongation at 72°C npr 0.75 233 40 20 30 (55) 30 108 Environmental DNA clone Fp nprI, Rp nprII ( 48 ) sub 0.75 319 40 20 30 (55) 30 88 Bacillus subtilis ATCC 6051 Fp subIa, RP subII ( 48 ) chiA 0.5 417 40 60 60 (55) 60 110 Stenotrophomonas rhizophila ATCC BAA 473 GA1F, GA1R ( 70 ) ureC 0.5 317 35 60 60 (60) 120 92 Pseudomonas chlororaphis O6 ureC1F, ureC2R ( 71 ) Open in a separate window Real-time PCR amplification conditions, efficiencies, calibration standard, and primers Soil metagenome processing and gene-targeted assembly.

    Techniques: Real-time Polymerase Chain Reaction, Amplification