mouse pituitary tumor att 20  (ATCC)

Journal: Endocrinology

Article Title: miR-375 Regulation of SSTR2 Expression in Corticotroph Pituitary Cells: Somatostatin Receptor Ligands Effects

doi: 10.1210/endocr/bqaf107

Figure Lengend Snippet: (A) Expression of circulating miR-375 in sera of 17 normal subjects and 26 patients with CD. (B) Expression of miR-375 in 2 normal pituitaries and 6 corticotroph pituitary adenomas tissues. (C) Expression of miR-375 in GH3 and AtT20/D16 cell lines. (D) Expression of miR-375 in AtT20/D16 cell line under DEX 10 −8 M treatment after 24 hours, 48 hours and 6 days. (E) Sstr2 gene expression in GH3 and AtT20/D16 cell lines. (F) Sstr2 gene expression modulation by DEX 10 −8 M treatment after 24 hours, 48 hours, and 6 days in AtT20/D16 cell line. Data in the graphs performed in cell lines represent mean ± SEM of 3 independent experiments. * P < .05, ** P < .01, *** P < .001, **** P < .0001 vs control or among the groups.

Article Snippet: AtT20/D16 cell line (CRL-1795), a strain of the mouse corticotroph pituitary tumor model AtT20, and GH3 cell line (CCL-82.1), a rat somatotroph pituitary tumor model, were provided by American Type Culture Collection (ATCC) and cultured in DMEM media supplemented with 10% fetal bovine serum (FBS) (Gibco, code number 16140071), 200 mM L-glutamine (Gibco, code number 25030081) and 1 × 10 5 U/L penicillin and streptomycin (Gibco, code number 15140122).

Techniques: Expressing, Gene Expression, Control

Journal: Endocrinology

Article Title: miR-375 Regulation of SSTR2 Expression in Corticotroph Pituitary Cells: Somatostatin Receptor Ligands Effects

doi: 10.1210/endocr/bqaf107

Figure Lengend Snippet: (A) Scheme of seed match between miR-375 and Sstr2 in mouse and SSTR2 in human. (B) Protein expression and densitometry of SSTR2 in AtT20/D16 treated with miR-375 inhibitor and mimic, evaluated by WB. The data in the graphs are expressed as a percentage of control and represent the mean ± SEM of 8 independent experiments. (C) Protein expression and fluorescence intensity of SSTR2 in AtT20/D16 treated with miR-375 inhibitor and mimic and in 2 human corticotroph primary cultures and (D) treated with miR-375 inhibitor evaluated by IF. The data in the graphs are expressed as a percentage of control normalized to cell number and represent the mean ± SEM of 3 independent experiments in cell lines. * P < .05, ** P < .01, *** P < .001, **** P < .0001 vs control or among the groups.

Article Snippet: AtT20/D16 cell line (CRL-1795), a strain of the mouse corticotroph pituitary tumor model AtT20, and GH3 cell line (CCL-82.1), a rat somatotroph pituitary tumor model, were provided by American Type Culture Collection (ATCC) and cultured in DMEM media supplemented with 10% fetal bovine serum (FBS) (Gibco, code number 16140071), 200 mM L-glutamine (Gibco, code number 25030081) and 1 × 10 5 U/L penicillin and streptomycin (Gibco, code number 15140122).

Techniques: Expressing, Control, Fluorescence

Journal: Endocrinology

Article Title: miR-375 Regulation of SSTR2 Expression in Corticotroph Pituitary Cells: Somatostatin Receptor Ligands Effects

doi: 10.1210/endocr/bqaf107

Figure Lengend Snippet: Evaluation of cell proliferation in AtT20/D16 treated with (A) OCT 10 −8 M and 10 −7 M and (B) PAS 10 −8 M and 10 −7 M with and without miR-375 inhibitors after 3 days of treatment by DNA assay. The data in the graphs are expressed as a percentage of control and represent the mean ± SEM of 3 independent experiments. * P < .05, ** P < .01, *** P < .001, **** P < .0001 among the groups.

Article Snippet: AtT20/D16 cell line (CRL-1795), a strain of the mouse corticotroph pituitary tumor model AtT20, and GH3 cell line (CCL-82.1), a rat somatotroph pituitary tumor model, were provided by American Type Culture Collection (ATCC) and cultured in DMEM media supplemented with 10% fetal bovine serum (FBS) (Gibco, code number 16140071), 200 mM L-glutamine (Gibco, code number 25030081) and 1 × 10 5 U/L penicillin and streptomycin (Gibco, code number 15140122).

Techniques: Control

Journal: Endocrinology

Article Title: miR-375 Regulation of SSTR2 Expression in Corticotroph Pituitary Cells: Somatostatin Receptor Ligands Effects

doi: 10.1210/endocr/bqaf107

Figure Lengend Snippet: Cellular localization of SSTR2 protein expression in AtT20/D16 evaluated by IF in the presence of OCT 10 −7 M for 20 minutes, miR-375 inhibitor and their combination. Images were captured at 60× magnification. The images reported in the figure represent 1 of the 3 independent experiments.

Article Snippet: AtT20/D16 cell line (CRL-1795), a strain of the mouse corticotroph pituitary tumor model AtT20, and GH3 cell line (CCL-82.1), a rat somatotroph pituitary tumor model, were provided by American Type Culture Collection (ATCC) and cultured in DMEM media supplemented with 10% fetal bovine serum (FBS) (Gibco, code number 16140071), 200 mM L-glutamine (Gibco, code number 25030081) and 1 × 10 5 U/L penicillin and streptomycin (Gibco, code number 15140122).

Techniques: Expressing

Journal: Endocrinology

Article Title: miR-375 Regulation of SSTR2 Expression in Corticotroph Pituitary Cells: Somatostatin Receptor Ligands Effects

doi: 10.1210/endocr/bqaf107

Figure Lengend Snippet: Intracellular signaling modulation of proliferation markers, ERK1/2, and apoptotic markers, caspase 3, and PARP, in AtT20/D16 treated with OCT 10 −8 M and 10 −7 M, miR-375 inhibitor, and their combination, evaluated by WB.

Article Snippet: AtT20/D16 cell line (CRL-1795), a strain of the mouse corticotroph pituitary tumor model AtT20, and GH3 cell line (CCL-82.1), a rat somatotroph pituitary tumor model, were provided by American Type Culture Collection (ATCC) and cultured in DMEM media supplemented with 10% fetal bovine serum (FBS) (Gibco, code number 16140071), 200 mM L-glutamine (Gibco, code number 25030081) and 1 × 10 5 U/L penicillin and streptomycin (Gibco, code number 15140122).

Techniques: