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autophagy related 7 atg7  (Proteintech)


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    Proteintech autophagy related 7 atg7
    Autophagy Related 7 Atg7, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1517 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/autophagy related 7 atg7/product/Proteintech
    Average 96 stars, based on 1517 article reviews
    autophagy related 7 atg7 - by Bioz Stars, 2026-02
    96/100 stars

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    Increased IL-1β secretion by macrophages due to autophagy impairment enhances inflammatory chemokine expression in LSECs. ( A ) Protein expression in ATG7-KO macrophages and NC macrophages generated using the CRISPR-CAS9 system. ( B ) Evaluation of IL-1β and caspase-1 levels in the culture supernatant of THP-1 macrophages by ELISA following 24-hour stimulation with or without 25 ng/mL LPS (n = 3/group). ( C ) Experimental method using LPS-stimulated THP-1 macrophage conditioned medium. ( D ) Evaluation of NF-κB or MAP kinase-related proteins in TMNK-1 cells. ( E and F ) Inflammatory chemokine gene expression in TMNK-1 cells when treated with control medium or THP-1 macrophage conditioned medium (n = 4/group). SN50, NF-κB inhibitor; SP600125, JNK inhibitor; SB203580, p38 MAPK inhibitor. ( G ) Inflammatory chemokine gene expression measured 24 hours after treating TMNK-1 cells with varying concentrations of human recombinant IL-1β (n = 4/group). ( H ) Evaluation of inflammatory chemokine expression in TMNK-1 cells after 6 hours of stimulation with recombinant human IL-1β (5 ng/mL) in the presence of SP600125 and SB203580 (n = 4/group).

    Journal: Cellular and Molecular Gastroenterology and Hepatology

    Article Title: Liver Sinusoidal Endothelial Cells Promote Metabolic Dysfunction-associated Steatohepatitis Progression via Interleukin-1R1-mediated Chemokine Production Induced by Macrophage-derived Interleukin-1β

    doi: 10.1016/j.jcmgh.2025.101698

    Figure Lengend Snippet: Increased IL-1β secretion by macrophages due to autophagy impairment enhances inflammatory chemokine expression in LSECs. ( A ) Protein expression in ATG7-KO macrophages and NC macrophages generated using the CRISPR-CAS9 system. ( B ) Evaluation of IL-1β and caspase-1 levels in the culture supernatant of THP-1 macrophages by ELISA following 24-hour stimulation with or without 25 ng/mL LPS (n = 3/group). ( C ) Experimental method using LPS-stimulated THP-1 macrophage conditioned medium. ( D ) Evaluation of NF-κB or MAP kinase-related proteins in TMNK-1 cells. ( E and F ) Inflammatory chemokine gene expression in TMNK-1 cells when treated with control medium or THP-1 macrophage conditioned medium (n = 4/group). SN50, NF-κB inhibitor; SP600125, JNK inhibitor; SB203580, p38 MAPK inhibitor. ( G ) Inflammatory chemokine gene expression measured 24 hours after treating TMNK-1 cells with varying concentrations of human recombinant IL-1β (n = 4/group). ( H ) Evaluation of inflammatory chemokine expression in TMNK-1 cells after 6 hours of stimulation with recombinant human IL-1β (5 ng/mL) in the presence of SP600125 and SB203580 (n = 4/group).

    Article Snippet: , Atg7 , Mm00512209_m1 , , , .

    Techniques: Expressing, Generated, CRISPR, Enzyme-linked Immunosorbent Assay, Gene Expression, Control, Recombinant

    Myeloid-specific Atg7 deficiency is associated with increased levels of mature IL-1β in liver tissue and increased chemokine expression in LSECs. ( A ) Evaluation of mature IL-1β protein levels in liver tissues from Atg7 flox/flox or Atg7 ΔMye mice in LPS intraperitoneal injection model mice (n = 6/group). ( B ) Atg7 gene expression in cells isolated from liver tissues of Atg7 flox/flox or Atg7 ΔMye mice (n = 10–12/group). ( C ) Expression of inflammatory chemokine genes in liver tissues from model mice intraperitoneally injected with LPS (n = 13–14 mice/group). ( D ) Expression of inflammatory chemokine genes in isolated LSECs (n = 9–12/group).

    Journal: Cellular and Molecular Gastroenterology and Hepatology

    Article Title: Liver Sinusoidal Endothelial Cells Promote Metabolic Dysfunction-associated Steatohepatitis Progression via Interleukin-1R1-mediated Chemokine Production Induced by Macrophage-derived Interleukin-1β

    doi: 10.1016/j.jcmgh.2025.101698

    Figure Lengend Snippet: Myeloid-specific Atg7 deficiency is associated with increased levels of mature IL-1β in liver tissue and increased chemokine expression in LSECs. ( A ) Evaluation of mature IL-1β protein levels in liver tissues from Atg7 flox/flox or Atg7 ΔMye mice in LPS intraperitoneal injection model mice (n = 6/group). ( B ) Atg7 gene expression in cells isolated from liver tissues of Atg7 flox/flox or Atg7 ΔMye mice (n = 10–12/group). ( C ) Expression of inflammatory chemokine genes in liver tissues from model mice intraperitoneally injected with LPS (n = 13–14 mice/group). ( D ) Expression of inflammatory chemokine genes in isolated LSECs (n = 9–12/group).

    Article Snippet: , Atg7 , Mm00512209_m1 , , , .

    Techniques: Expressing, Injection, Gene Expression, Isolation