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ascl4  (Proteintech)


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    Proteintech ascl4
    Ascl4, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 283 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ascl4/ACSL4+Antibody/pmc12305878-11-0-6
    Average 96 stars, based on 283 article reviews
    ascl4 - by Bioz Stars, 2026-11
    96/100 stars

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    other:

    Article Title: PCK2 induces gefitinib resistance by suppresses ferroptosis in non-small cell lung cancer.
    Article Snippet: Objectives: This study aimed to explore the involvement of phosphoenolpyruvate carboxykinase 2 (PCK2) in gefitinib-resistant non-small cell lung cancer (NSCLC) cells and assess its feasibility as a therapeutic target against gefitinib resistance.. Methods: Gefitinib-resistant cell lines, PC9GR and HCC827GR, were generated through progressive exposure of parental cells to escalating concentrations of gefitinib.. Transcriptomic analysis encompassed the treatment of PC9 and PC9GR cells with gefitinib or vehicle, followed by RNA extraction, sequencing, and subsequent bioinformatic analysis.

    Article Title: Golgi protein ACBD3 downregulation sensitizes cells to ferroptosis.
    Article Snippet: School of Life Sciences, Anhui Medical University, Hefei, China State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China Department of Wound Repair and Plastic and Aesthetic Surgery, the First Affiliated Hospital of Anhui Medical University, Hefei, China Anhui Public Health Clinical Center, Hefei, China Anhui Provincial Institute of Translational Medicine, Anhui Medical University, Hefei, China

    Article Title: Alpha-linolenic acid protects against heatstroke-induced acute lung injury by inhibiting ferroptosis through Nrf2 activation
    Article Snippet: ASCL4 , 66617-1-Ig , 1:1000 , Proteintech , 70KDa.

    Blocking Assay:

    Article Title: FTH1 overexpression using a dCasRx translation enhancement system protects the kidney from calcium oxalate crystal-induced injury
    Article Snippet: .. The proteins were subsequently transferred onto Immun-Blot polyvinylidene fluoride (PVDF) membranes (1620177, BIO-RAD, USA).Membrane blocking was accomplished by applying a rapid blocking buffer (PS108P, Epizyme, China) for 10 min, then proceeding with multiple washes in Tris-buffered saline containing Tween 20 (TBST).Afterward, the membranes were left to incubate overnight at 4 °C with primary antibodies that targeted FTH1 (ab65080, 1:1000, Abcam, UK), GPX4 (A1933, 1:1000, ABclonal, China), CD71 (A5865, 1:1,000, ABclonal, China), ASCL4 (22401-1-AP, 1:4000, Proteintech, USA), SIRT1 (13161-1-AP, 1:2,000, Proteintech, USA), NIX (A24803, 1:1000, ABclonal, China), Tubulin (11224-1-AP, 1:10,000, Proteintech, USA), and GAPDH (10494-1-AP, 1:20,000, Proteintech, USA). .. After additional TBST washes, the membranes were then exposed to suitable secondary antibodies at room temperature for a duration of 1 h. Protein bands were observed with an Odyssey dual-color infrared laser imager from LI-COR in the USA, and the intensities of the bands were measured using ImageJ software version 1.51j8.

    Saline:

    Article Title: FTH1 overexpression using a dCasRx translation enhancement system protects the kidney from calcium oxalate crystal-induced injury
    Article Snippet: .. The proteins were subsequently transferred onto Immun-Blot polyvinylidene fluoride (PVDF) membranes (1620177, BIO-RAD, USA).Membrane blocking was accomplished by applying a rapid blocking buffer (PS108P, Epizyme, China) for 10 min, then proceeding with multiple washes in Tris-buffered saline containing Tween 20 (TBST).Afterward, the membranes were left to incubate overnight at 4 °C with primary antibodies that targeted FTH1 (ab65080, 1:1000, Abcam, UK), GPX4 (A1933, 1:1000, ABclonal, China), CD71 (A5865, 1:1,000, ABclonal, China), ASCL4 (22401-1-AP, 1:4000, Proteintech, USA), SIRT1 (13161-1-AP, 1:2,000, Proteintech, USA), NIX (A24803, 1:1000, ABclonal, China), Tubulin (11224-1-AP, 1:10,000, Proteintech, USA), and GAPDH (10494-1-AP, 1:20,000, Proteintech, USA). .. After additional TBST washes, the membranes were then exposed to suitable secondary antibodies at room temperature for a duration of 1 h. Protein bands were observed with an Odyssey dual-color infrared laser imager from LI-COR in the USA, and the intensities of the bands were measured using ImageJ software version 1.51j8.



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    MedChemExpress ascl4
    A , B Western blot analyses of NCOA4 protein in HSC-T6 cells in NC or siNCOA4 group. C , D Expression of α-SMA, Desmin, and FTH by immunofluorescence staining in NC or siNCOA4 HSC-T6 cells treated with Ag, and the quantitative analysis. Scale bar = 10 or 50 μm. E HSC-T6 cells captured by TEM. The green arrows indicate the damaged mitochondria. The yellow arrows indicate the healthy mitochondria. The red arrows stand for autolysosome. F Quantitative PCR analyses of NCOA4 mRNA expression in activated JS-1 cells in NC or siNCOA4 group. G , H Western blot analyses of P62 and LC3B II protein in NC or siNCOA4 JS-1 cells treated with TGF-β1 or/and Ag, and the quantitative analysis. I and J Western blot analyses of <t>ASCL4,</t> XCT, and GPX4 protein in NC or siNCOA4 JS-1 cells treated with TGF-β1 or/and Ag, and the quantitative analysis. K JS-1 cells captured by TEM. L , M Expression of α-SMA by immunofluorescence staining in JS-1 cells in different groups, and the quantitative analysis. Scale bar = 20 μm. N Quantitative PCR analyses of FTH and FTL mRNA expression in JS-1 cells. All data presented as means ± SEM. n = 3–6 per group. (ns means p > 0.05, * p < 0.05, ** p < 0.01, ***p < 0.001, **** p < 0.0001).
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    anti ascl4 antibody  (ABclonal Biotechnology)
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    A , B Western blot analyses of NCOA4 protein in HSC-T6 cells in NC or siNCOA4 group. C , D Expression of α-SMA, Desmin, and FTH by immunofluorescence staining in NC or siNCOA4 HSC-T6 cells treated with Ag, and the quantitative analysis. Scale bar = 10 or 50 μm. E HSC-T6 cells captured by TEM. The green arrows indicate the damaged mitochondria. The yellow arrows indicate the healthy mitochondria. The red arrows stand for autolysosome. F Quantitative PCR analyses of NCOA4 mRNA expression in activated JS-1 cells in NC or siNCOA4 group. G , H Western blot analyses of P62 and LC3B II protein in NC or siNCOA4 JS-1 cells treated with TGF-β1 or/and Ag, and the quantitative analysis. I and J Western blot analyses of <t>ASCL4,</t> XCT, and GPX4 protein in NC or siNCOA4 JS-1 cells treated with TGF-β1 or/and Ag, and the quantitative analysis. K JS-1 cells captured by TEM. L , M Expression of α-SMA by immunofluorescence staining in JS-1 cells in different groups, and the quantitative analysis. Scale bar = 20 μm. N Quantitative PCR analyses of FTH and FTL mRNA expression in JS-1 cells. All data presented as means ± SEM. n = 3–6 per group. (ns means p > 0.05, * p < 0.05, ** p < 0.01, ***p < 0.001, **** p < 0.0001).
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    Image Search Results


    A , B Western blot analyses of NCOA4 protein in HSC-T6 cells in NC or siNCOA4 group. C , D Expression of α-SMA, Desmin, and FTH by immunofluorescence staining in NC or siNCOA4 HSC-T6 cells treated with Ag, and the quantitative analysis. Scale bar = 10 or 50 μm. E HSC-T6 cells captured by TEM. The green arrows indicate the damaged mitochondria. The yellow arrows indicate the healthy mitochondria. The red arrows stand for autolysosome. F Quantitative PCR analyses of NCOA4 mRNA expression in activated JS-1 cells in NC or siNCOA4 group. G , H Western blot analyses of P62 and LC3B II protein in NC or siNCOA4 JS-1 cells treated with TGF-β1 or/and Ag, and the quantitative analysis. I and J Western blot analyses of ASCL4, XCT, and GPX4 protein in NC or siNCOA4 JS-1 cells treated with TGF-β1 or/and Ag, and the quantitative analysis. K JS-1 cells captured by TEM. L , M Expression of α-SMA by immunofluorescence staining in JS-1 cells in different groups, and the quantitative analysis. Scale bar = 20 μm. N Quantitative PCR analyses of FTH and FTL mRNA expression in JS-1 cells. All data presented as means ± SEM. n = 3–6 per group. (ns means p > 0.05, * p < 0.05, ** p < 0.01, ***p < 0.001, **** p < 0.0001).

    Journal: Communications Biology

    Article Title: Astragalin promotes HSCs ferroptosis through NCOA4 mediated ferritinophagy to alleviate liver fibrosis in zebrafish and mice

    doi: 10.1038/s42003-025-08421-0

    Figure Lengend Snippet: A , B Western blot analyses of NCOA4 protein in HSC-T6 cells in NC or siNCOA4 group. C , D Expression of α-SMA, Desmin, and FTH by immunofluorescence staining in NC or siNCOA4 HSC-T6 cells treated with Ag, and the quantitative analysis. Scale bar = 10 or 50 μm. E HSC-T6 cells captured by TEM. The green arrows indicate the damaged mitochondria. The yellow arrows indicate the healthy mitochondria. The red arrows stand for autolysosome. F Quantitative PCR analyses of NCOA4 mRNA expression in activated JS-1 cells in NC or siNCOA4 group. G , H Western blot analyses of P62 and LC3B II protein in NC or siNCOA4 JS-1 cells treated with TGF-β1 or/and Ag, and the quantitative analysis. I and J Western blot analyses of ASCL4, XCT, and GPX4 protein in NC or siNCOA4 JS-1 cells treated with TGF-β1 or/and Ag, and the quantitative analysis. K JS-1 cells captured by TEM. L , M Expression of α-SMA by immunofluorescence staining in JS-1 cells in different groups, and the quantitative analysis. Scale bar = 20 μm. N Quantitative PCR analyses of FTH and FTL mRNA expression in JS-1 cells. All data presented as means ± SEM. n = 3–6 per group. (ns means p > 0.05, * p < 0.05, ** p < 0.01, ***p < 0.001, **** p < 0.0001).

    Article Snippet: Primary antibodies including α-SMA (1:400, proteintech), Desmin (HuaBio, 1:200), Collagen-1 (Abcam, 1:400), LC3B II (Abcam, 1:200), P62 (Abmart, 1:200), NCOA4 (DF4255, SAB, 1:100), GPX4 (ab125066, Abcam, 1:500), COX2 (ab283574, Abcam, 1:500), FTH (1:100, Abcam), ASCL4 (HY- P80977 , MCE, 1:100).

    Techniques: Western Blot, Expressing, Immunofluorescence, Staining, Real-time Polymerase Chain Reaction

    A , B Immunofluorescence staining of α-SMA, TFR, DMT1, and GPX4 in HSC-T6 cells treated with Ag as well as vehicle or Fer-1, and the quantitative analysis. Scale bar = 10 μm. C , D ROS probe staining and Fe 2+ probe staining of HSC-T6 cells in different groups, and the quantitative analysis. Scale bar= 10 or 100 μm. E MDA assay of HSC-T6 cells in different groups. F , G Immunofluorescence staining of α-SMA in JS-1 cells in different groups, and the quantitative analysis. Scale bar = 20 μm. H Quantitative PCR analyses of GPX4 , COX2 and TFR mRNA expression in JS-1 cells. I , J Western blot analyses of ASCL4 and DMT1 protein in JS-1 cells, and the quantitative analysis. K , L Western blot analyses of LC3B II, P62, FTH and FTL protein in JS-1 cells, and the quantitative analysis. All data presented as means ± SEM. n = 3–5 per group. (ns means p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

    Journal: Communications Biology

    Article Title: Astragalin promotes HSCs ferroptosis through NCOA4 mediated ferritinophagy to alleviate liver fibrosis in zebrafish and mice

    doi: 10.1038/s42003-025-08421-0

    Figure Lengend Snippet: A , B Immunofluorescence staining of α-SMA, TFR, DMT1, and GPX4 in HSC-T6 cells treated with Ag as well as vehicle or Fer-1, and the quantitative analysis. Scale bar = 10 μm. C , D ROS probe staining and Fe 2+ probe staining of HSC-T6 cells in different groups, and the quantitative analysis. Scale bar= 10 or 100 μm. E MDA assay of HSC-T6 cells in different groups. F , G Immunofluorescence staining of α-SMA in JS-1 cells in different groups, and the quantitative analysis. Scale bar = 20 μm. H Quantitative PCR analyses of GPX4 , COX2 and TFR mRNA expression in JS-1 cells. I , J Western blot analyses of ASCL4 and DMT1 protein in JS-1 cells, and the quantitative analysis. K , L Western blot analyses of LC3B II, P62, FTH and FTL protein in JS-1 cells, and the quantitative analysis. All data presented as means ± SEM. n = 3–5 per group. (ns means p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

    Article Snippet: Primary antibodies including α-SMA (1:400, proteintech), Desmin (HuaBio, 1:200), Collagen-1 (Abcam, 1:400), LC3B II (Abcam, 1:200), P62 (Abmart, 1:200), NCOA4 (DF4255, SAB, 1:100), GPX4 (ab125066, Abcam, 1:500), COX2 (ab283574, Abcam, 1:500), FTH (1:100, Abcam), ASCL4 (HY- P80977 , MCE, 1:100).

    Techniques: Immunofluorescence, Staining, Multiple Displacement Amplification, Real-time Polymerase Chain Reaction, Expressing, Western Blot