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tms1/asc antibody  (MedChemExpress)


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    Structured Review

    MedChemExpress tms1/asc antibody
    Tms1/Asc Antibody, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/asc/TMS1%2FASC+Antibody/custom%40hy-p80548%4042099108
    Average 94 stars, based on 2 article reviews
    tms1/asc antibody - by Bioz Stars, 2026-09
    94/100 stars

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    Incubation:

    Article Title: Endothelial progenitor cell-derived microvesicles therapy relieves myocardial infarction symptoms by altering left ventricular protein expression
    Article Snippet: .. The membranes were incubated overnight at 4°C with primary antibodies targeting: CD34 (1:1000, Abcam, ab317588), Bcl-2 (1:2000, Abcam, ab182858), Bax (1:1000, Abcam, ab32503), Cleaved Caspase-3 (1:500, Abcam, ab2302), NLRP3 (1:1000, GeneTex, GTX639954), Caspase-1 (1:1000, GeneTex, GTX101322), ASC (1:1500, MCE, HY- P80548 ), IL-6 (1:1000, Abcam, ab290735), NPM1 (1:1000, Abcam, ab52644), CSRP3 (1:1000, Abcam, ab172952), NPPA (1:1000, Abcam, ab225844), CCL23 (1:1000, Thermo Fisher Scientific, 500-P124-1MG), and β-actin (1:1000, Abcam, ab8226). ..

    Article Title: Exosomes derived from diabetic microenvironment-preconditioned mesenchymal stem cells ameliorate nonalcoholic fatty liver disease and inhibit pyroptosis of hepatocytes.
    Article Snippet: .. Fixed HepG2 cells and frozen liver sections were blocked with 10 % goat serum at RT for 30 min and 1 h, respectively, and incubated with primary antibodies against GSDMD (#20770-1-AP, 1:100, Proteintech), Caspase-1 (#22915-1-AP, 1:25, Proteintech), ASC (#67824, 1:800, CST; #HY-P80548, 1:50, MCE), and IL-1β (#HY-P80720, 1:100, MCE) overnight at 4 ◦C. .. Cells or liver sections were next incubated with a goat antirabbit IgG (H + L) conjugated to Alexa FluorTM 594 (#A-11012, Invitrogen, Carlsbad, CA, USA) secondary antibody at RT for 1 h. Finally, 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich, St. Louis, MO, USA) was applied to stain nuclei.

    Article Title: Methylene blue mitigates lung injury in HCA rats by regulating macrophage pyroptosis via Nrf2/HO-1 and NLRP3 pathways.
    Article Snippet: .. The samples were then subjected to electrophoresis on 12% SDS-PAGE gels (Invitrogen), transferred onto polyvinylidene difluoride (PVDF) membranes (Invitrogen), and blocked with 5% BSA for 3 h. After washing, the membranes were incubated overnight at 4°C with the following primary antibodies: Nrf2 (PA5-27882, 1:500, Invitrogen), HO-1 (PA5-77833, 1:1000, Invitrogen), NLRP3 (MA5-23919, 1:100, Invitrogen), pro-caspase-1 (ZRB1233, 1:1000, Sigma-Aldrich), caspase-1 (MA5-16215, 1:200, Invitrogen), ASC (HY-P80548, 1:500, MedChemExpress), GSDMD-N (ab215203, 1:1000, Abcam), or GSDMD (PA5-116815, 1:1000, Invitrogen). .. The next day, membranes were incubated with a secondary antibody (ab205718, 1:10,000, Abcam) for 2 hours.

    Article Title: Luteolin Ameliorates Allergic Rhinitis in Mice through Modulating T Cell Subset Imbalance, Endoplasmic Reticulum Stress, and NLRP3 Inflammasome Axes.
    Article Snippet: After separation using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) (12%, Invitrogen), the samples were shifted to a polyvinylidene fluoride (PVDF) membrane (Invitrogen), followed by blocking for 3 hours. .. After rinsing the membranes, placed them at 4°C for an overnight incubation with associated primary antibodies NLRP3 (ab4207, 1:1000, Abcam), ASC (HY-P80548, 1:1000, MedChemExpress, Monmouth Junction, NJ, USA), caspase 1 (ab138483, 1:1000, Abcam), IL-1β (1:2000), IL-18 (1:400), C/EBP homologous protein (CHOP, MA1-250, 1:2000, Invitrogen), glucoseregulated protein 78 (GRP78, PA1-014A, 1:100, Invitrogen), eukaryotic initiation factor 2alpha (eIF2α, ab169528, 1:2000, Abcam), activating transcription factor 4 (ATF4, ab85049, 1:1000, Abcam), and GAPDH (MA5-15738, 1:1000, Invitrogen). .. On the following day, after being rinsed thrice, the membranes were cultured with goat anti-mouse IgG (ab205719, 1:20 000, Abcam) for 2 hours.

    Article Title: Methylene blue mitigates lung injury in HCA rats by regulating macrophage pyroptosis via Nrf2/HO-1 and NLRP3 pathways
    Article Snippet: .. The samples were then subjected to electrophoresis on 12% SDS-PAGE gels (Invitrogen), transferred onto polyvinylidene difluoride (PVDF) membranes (Invitrogen), and blocked with 5% BSA for 3 h. After washing, the membranes were incubated overnight at 4 ∘ C with the following primary antibodies: Nrf2 (PA5-27882, 1:500, Invitrogen), HO-1 (PA5-77833, 1:1000, Invitrogen), NLRP3 (MA5-23919, 1:100, Invitrogen), pro-caspase-1 (ZRB1233, 1:1000, Sigma-Aldrich), caspase-1 (MA5-16215, 1:200, Invitrogen), ASC (HY- P80548 , 1:500, MedChemExpress), GSDMD-N (ab215203, 1:1000, Abcam), or GSDMD (PA5-116815, 1:1000, Invitrogen). .. The next day, membranes were incubated with a secondary antibody (ab205718, 1:10,000, Abcam) for 2 h. A chemiluminescent substrate (ECL, 34580, Thermo Fisher Scientific) was evenly applied to the membranes, and protein bands were visualized using a gel imaging system (iBright CL1500, Invitrogen).

    Article Title: Gastrodin alleviates bortezomib-induced peripheral neuropathy by inhibiting NF-κB/NLRP3 pathway-mediated microglial inflammation
    Article Snippet: .. After blocking at room temperature for 2 hour, membranes were incubated overnight at 4°C with primary antibodies: p-NF-κB p65 (Ser536, 1:1000; Signalway), NLRP3 (SC06-23, 1:1500; HUABIO), ASC (HY- P80548 ; MCE), NF-κB p65 (L8F6, 1:500; Cell Signaling), caspase-1 (SU40-07, 1:80,000; HUABIO), cleaved-caspase-1 (Asp296, 1:1000; Affinity), and GAPDH (60004-1-Ig, 1:50000; Proteintech). .. On the following day, membranes were washed and exposed to secondary antibodies (SA00001-1; SA00001-2, 1:5000; Proteintech) for 1 hour at room temperature, and signals were subsequently detected using ECL chemiluminescent solution (BMU102; Abbkine).

    Electrophoresis:

    Article Title: Methylene blue mitigates lung injury in HCA rats by regulating macrophage pyroptosis via Nrf2/HO-1 and NLRP3 pathways.
    Article Snippet: .. The samples were then subjected to electrophoresis on 12% SDS-PAGE gels (Invitrogen), transferred onto polyvinylidene difluoride (PVDF) membranes (Invitrogen), and blocked with 5% BSA for 3 h. After washing, the membranes were incubated overnight at 4°C with the following primary antibodies: Nrf2 (PA5-27882, 1:500, Invitrogen), HO-1 (PA5-77833, 1:1000, Invitrogen), NLRP3 (MA5-23919, 1:100, Invitrogen), pro-caspase-1 (ZRB1233, 1:1000, Sigma-Aldrich), caspase-1 (MA5-16215, 1:200, Invitrogen), ASC (HY-P80548, 1:500, MedChemExpress), GSDMD-N (ab215203, 1:1000, Abcam), or GSDMD (PA5-116815, 1:1000, Invitrogen). .. The next day, membranes were incubated with a secondary antibody (ab205718, 1:10,000, Abcam) for 2 hours.

    Article Title: Methylene blue mitigates lung injury in HCA rats by regulating macrophage pyroptosis via Nrf2/HO-1 and NLRP3 pathways
    Article Snippet: .. The samples were then subjected to electrophoresis on 12% SDS-PAGE gels (Invitrogen), transferred onto polyvinylidene difluoride (PVDF) membranes (Invitrogen), and blocked with 5% BSA for 3 h. After washing, the membranes were incubated overnight at 4 ∘ C with the following primary antibodies: Nrf2 (PA5-27882, 1:500, Invitrogen), HO-1 (PA5-77833, 1:1000, Invitrogen), NLRP3 (MA5-23919, 1:100, Invitrogen), pro-caspase-1 (ZRB1233, 1:1000, Sigma-Aldrich), caspase-1 (MA5-16215, 1:200, Invitrogen), ASC (HY- P80548 , 1:500, MedChemExpress), GSDMD-N (ab215203, 1:1000, Abcam), or GSDMD (PA5-116815, 1:1000, Invitrogen). .. The next day, membranes were incubated with a secondary antibody (ab205718, 1:10,000, Abcam) for 2 h. A chemiluminescent substrate (ECL, 34580, Thermo Fisher Scientific) was evenly applied to the membranes, and protein bands were visualized using a gel imaging system (iBright CL1500, Invitrogen).

    SDS Page:

    Article Title: Methylene blue mitigates lung injury in HCA rats by regulating macrophage pyroptosis via Nrf2/HO-1 and NLRP3 pathways.
    Article Snippet: .. The samples were then subjected to electrophoresis on 12% SDS-PAGE gels (Invitrogen), transferred onto polyvinylidene difluoride (PVDF) membranes (Invitrogen), and blocked with 5% BSA for 3 h. After washing, the membranes were incubated overnight at 4°C with the following primary antibodies: Nrf2 (PA5-27882, 1:500, Invitrogen), HO-1 (PA5-77833, 1:1000, Invitrogen), NLRP3 (MA5-23919, 1:100, Invitrogen), pro-caspase-1 (ZRB1233, 1:1000, Sigma-Aldrich), caspase-1 (MA5-16215, 1:200, Invitrogen), ASC (HY-P80548, 1:500, MedChemExpress), GSDMD-N (ab215203, 1:1000, Abcam), or GSDMD (PA5-116815, 1:1000, Invitrogen). .. The next day, membranes were incubated with a secondary antibody (ab205718, 1:10,000, Abcam) for 2 hours.

    Article Title: Methylene blue mitigates lung injury in HCA rats by regulating macrophage pyroptosis via Nrf2/HO-1 and NLRP3 pathways
    Article Snippet: .. The samples were then subjected to electrophoresis on 12% SDS-PAGE gels (Invitrogen), transferred onto polyvinylidene difluoride (PVDF) membranes (Invitrogen), and blocked with 5% BSA for 3 h. After washing, the membranes were incubated overnight at 4 ∘ C with the following primary antibodies: Nrf2 (PA5-27882, 1:500, Invitrogen), HO-1 (PA5-77833, 1:1000, Invitrogen), NLRP3 (MA5-23919, 1:100, Invitrogen), pro-caspase-1 (ZRB1233, 1:1000, Sigma-Aldrich), caspase-1 (MA5-16215, 1:200, Invitrogen), ASC (HY- P80548 , 1:500, MedChemExpress), GSDMD-N (ab215203, 1:1000, Abcam), or GSDMD (PA5-116815, 1:1000, Invitrogen). .. The next day, membranes were incubated with a secondary antibody (ab205718, 1:10,000, Abcam) for 2 h. A chemiluminescent substrate (ECL, 34580, Thermo Fisher Scientific) was evenly applied to the membranes, and protein bands were visualized using a gel imaging system (iBright CL1500, Invitrogen).

    Control:

    Article Title: 4-Octyl itaconate-induced Nrf2 nuclear translocation mitigates Caspase-11-dependent noncanonical macrophage pyroptosis and acute lung injury.
    Article Snippet: .. The following primary antibodies were used: Caspase-11 (17D9; 1:1000, NB120-10454, NOVUS Biologicals, USA); GSDMD (EPR19828; 1:1000, ab209845, Abcam, UK); IL-1b (EPR16805-15; 1:1000, ab234437, Abcam, UK); Nrf2/NFE2L2 (1:1000, 80593–1-RR, Proteintech, China); HRP-conjugated bActin (AC028; 1:5000, ABclonal, China; used as a loading control); Lamin B1 Polyclonal antibody (1:2000, 12987–1-AP, Proteintech, China; used as a loading control); HRP-GAPDH (1:2000, AC035, ABclonal, China; used as a loading control); ASC (1:2000, HYP80548, MCE). .. After three washes with TBST, the membranes were incubated with HRP-conjugated secondary antibodies: goat antirabbit IgG (H + L) (1:5000, 31460, Thermo Fisher Scientific, USA) and goat anti-mouse IgG (H + L) (1:5000, S0007/S0011, Affinity Biosciences, USA).

    Blocking Assay:

    Article Title: Gastrodin alleviates bortezomib-induced peripheral neuropathy by inhibiting NF-κB/NLRP3 pathway-mediated microglial inflammation
    Article Snippet: .. After blocking at room temperature for 2 hour, membranes were incubated overnight at 4°C with primary antibodies: p-NF-κB p65 (Ser536, 1:1000; Signalway), NLRP3 (SC06-23, 1:1500; HUABIO), ASC (HY- P80548 ; MCE), NF-κB p65 (L8F6, 1:500; Cell Signaling), caspase-1 (SU40-07, 1:80,000; HUABIO), cleaved-caspase-1 (Asp296, 1:1000; Affinity), and GAPDH (60004-1-Ig, 1:50000; Proteintech). .. On the following day, membranes were washed and exposed to secondary antibodies (SA00001-1; SA00001-2, 1:5000; Proteintech) for 1 hour at room temperature, and signals were subsequently detected using ECL chemiluminescent solution (BMU102; Abbkine).



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