Review




Structured Review

Proteintech arg 1
Arg 1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 930 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/arg1/ARG1+Antibody/pm42822644-107-21-23
Average 96 stars, based on 930 article reviews
arg 1 - by Bioz Stars, 2026-10
96/100 stars

Images

Related Articles

Cell-Signaling:

Article Title: Biodegradable polyurethane nerve conduits functionalized with NDP-MSH-loaded self-assembling peptide hydrogels for peripheral nerve regeneration.
Article Snippet: Autologous nerve grafting remains the clinical gold standard for segmental peripheral nerve repair, but it is limited by donor-site morbidity and graft availability.. Nerve guidance conduits (NGCs) offer a promising alternative for peripheral nerve repair.. In this work, we engineered a fully biodegradable waterborne polyurethane (BWPU) NGC functionalized with a pH-responsive self-assembling peptide (SAP) loaded with [Nle4, D-Phe7]-a-melanocyte-stimulating hormone (NDP-MSH)—an a-MSH analogue that activates melanocortin receptors and has been reported to attenuate oxidative stress, apoptosis, and neuroinflammation.

Incubation:

Article Title: ZXDB Drives Macrophage Inflammatory Programming in Sepsis‐Induced Acute Kidney Injury by Recruiting EIF4A3 to Enhance ACACA Translation
Article Snippet: .. Membranes were then incubated overnight at 4°C with primary antibodies against: ZXDB (A303‐656A; Invitrogen, 1:1000), ACACA (21923‐1‐AP; Proteintech, RRID:AB_11042445, 1:1000), EIF4A3 (17 504‐1‐AP; Proteintech, RRID:AB_2097393, 1:1000), iNOS (22226‐1‐AP; Proteintech, RRID:AB_2879038, 1:1000), CD86 (13395‐1‐AP; Proteintech–, RRID:AB_2074882, 1:1000), ARG1 (16001‐1‐AP; Proteintech–, RRID:AB_2289842, 1:1000), CD206 (18704‐1‐AP; Proteintech, RRID:AB_10597232, 1:1000), GAPDH (60004‐1‐Ig; Proteintech, RRID:AB_2107436, 1:10000). ..

Article Title: A Forsythiaside A-loaded, oxygen-releasing thermosensitive hydrogel accelerates diabetic wound healing by remodeling the microenvironment
Article Snippet: For immunohistochemistry (IHC), paraffin-embedded sections were stained using the following primary antibodies: CK19 (Proteintech, 86250–1-RR), Collagen I (Proteintech, 14695–1-AP), and Collagen III (Proteintech, 22734–1-AP). .. For immunofluorescence (IF), tissue sections or cultured cells were incubated overnight at 4 ◦C with the following primary antibodies: CD31 (Proteintech, 11265–1-AP, dilution 1:200), αSMA (Servicbio, GB1113654, 1:500), F4/80 (Abcam, ab300421, 1:100), CD86 (Abclonal, A21198, 1:50), CD206 (Proteintech, 18704–1-AP, 1:100), Arg1 (Proteintech, 16001–1-AP, 1:200), and iNOS (Proteintech, 22226–1-AP, 1:200). .. After washing with PBS, sections were incubated for 1 h at room temperature in the dark with corresponding secondary antibodies: FITC- or Cy3conjugated goat anti-Rabbit IgG (Proteintech, SA00003-2, SA00009-2, dilution 1:200).

Article Title: MicroRNA-493-5p engineered exosomes delivered via piezoelectric microneedles for epigenetic modulation of macrophages in diabetic wound healing
Article Snippet: Proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes (Millipore, #ISEQ00010). .. After blocking with 5% skim milk, membranes were incubated overnight at 4 °C with primary antibodies against: Arg1 (Proteintech, #16001-1-AP), iNOS (Proteintech, #18985-1-AP), IL-1β (Proteintech, #16806-1-AP), PKM2 (ABclonal, #A19053), SLC7A11 (ABclonal, # A24146 ), HIF-1α (ABclonal, #A11945), and β-actin (Cell Signaling Technology, #4970). ..

Article Title: ACOX1 downregulation contributes to macrophage M1 polarization and neutrophilic airway inflammation in asthma.
Article Snippet: Separated proteins were transferred to PVDF membranes (Millipore), which were subsequently blocked with 5% skim milk for 1 h at room temperature. .. Membranes were incubated with primary antibodies against GAPDH (1:5000, Servicebio), Tubulin (1:5000, Servicebio), ACOX1 (1:2000, Abclonal), iNOS (1:2000, Abclonal), IL-1β (1:2000, Proteintech), ARG1(1:2000, Proteintech), ATGL(1:2000, Proteintech), p65/p-p65 (1:2000, Proteintech), and IKBα/p-IKBα (1:2000, Proteintech), followed by secondary antibodies. .. Protein bands were visualized using ECL substrate (MCE) and quantified using ImageJ software.

Immunofluorescence:

Article Title: A Forsythiaside A-loaded, oxygen-releasing thermosensitive hydrogel accelerates diabetic wound healing by remodeling the microenvironment
Article Snippet: For immunohistochemistry (IHC), paraffin-embedded sections were stained using the following primary antibodies: CK19 (Proteintech, 86250–1-RR), Collagen I (Proteintech, 14695–1-AP), and Collagen III (Proteintech, 22734–1-AP). .. For immunofluorescence (IF), tissue sections or cultured cells were incubated overnight at 4 ◦C with the following primary antibodies: CD31 (Proteintech, 11265–1-AP, dilution 1:200), αSMA (Servicbio, GB1113654, 1:500), F4/80 (Abcam, ab300421, 1:100), CD86 (Abclonal, A21198, 1:50), CD206 (Proteintech, 18704–1-AP, 1:100), Arg1 (Proteintech, 16001–1-AP, 1:200), and iNOS (Proteintech, 22226–1-AP, 1:200). .. After washing with PBS, sections were incubated for 1 h at room temperature in the dark with corresponding secondary antibodies: FITC- or Cy3conjugated goat anti-Rabbit IgG (Proteintech, SA00003-2, SA00009-2, dilution 1:200).

Article Title: Bacteria-responsive DNAgel system for targeted delivery of photothermally enhanced MXene/MoS 2 in the treatment of pyogenic osteomyelitis
Article Snippet: The PrimeScript RT reagent Kit with gDNA Eraser was purchased from Takara (Japan), and SYBR Green PCR Master Mix was purchased from Monad (China). .. Primary antibodies for immunocytochemistry and immunofluorescence staining—including ARG1 and iNOS, TRAP, β-Catenin, OCN and WNT4—were obtained from Proteintech (China). .. The IL-6, SP7, BMP2, and COL1A1 antibody was purchased from Abcam (UK).

Tissue:

Article Title: A Forsythiaside A-loaded, oxygen-releasing thermosensitive hydrogel accelerates diabetic wound healing by remodeling the microenvironment
Article Snippet: For immunohistochemistry (IHC), paraffin-embedded sections were stained using the following primary antibodies: CK19 (Proteintech, 86250–1-RR), Collagen I (Proteintech, 14695–1-AP), and Collagen III (Proteintech, 22734–1-AP). .. For immunofluorescence (IF), tissue sections or cultured cells were incubated overnight at 4 ◦C with the following primary antibodies: CD31 (Proteintech, 11265–1-AP, dilution 1:200), αSMA (Servicbio, GB1113654, 1:500), F4/80 (Abcam, ab300421, 1:100), CD86 (Abclonal, A21198, 1:50), CD206 (Proteintech, 18704–1-AP, 1:100), Arg1 (Proteintech, 16001–1-AP, 1:200), and iNOS (Proteintech, 22226–1-AP, 1:200). .. After washing with PBS, sections were incubated for 1 h at room temperature in the dark with corresponding secondary antibodies: FITC- or Cy3conjugated goat anti-Rabbit IgG (Proteintech, SA00003-2, SA00009-2, dilution 1:200).

Cell Culture:

Article Title: A Forsythiaside A-loaded, oxygen-releasing thermosensitive hydrogel accelerates diabetic wound healing by remodeling the microenvironment
Article Snippet: For immunohistochemistry (IHC), paraffin-embedded sections were stained using the following primary antibodies: CK19 (Proteintech, 86250–1-RR), Collagen I (Proteintech, 14695–1-AP), and Collagen III (Proteintech, 22734–1-AP). .. For immunofluorescence (IF), tissue sections or cultured cells were incubated overnight at 4 ◦C with the following primary antibodies: CD31 (Proteintech, 11265–1-AP, dilution 1:200), αSMA (Servicbio, GB1113654, 1:500), F4/80 (Abcam, ab300421, 1:100), CD86 (Abclonal, A21198, 1:50), CD206 (Proteintech, 18704–1-AP, 1:100), Arg1 (Proteintech, 16001–1-AP, 1:200), and iNOS (Proteintech, 22226–1-AP, 1:200). .. After washing with PBS, sections were incubated for 1 h at room temperature in the dark with corresponding secondary antibodies: FITC- or Cy3conjugated goat anti-Rabbit IgG (Proteintech, SA00003-2, SA00009-2, dilution 1:200).

Blocking Assay:

Article Title: MicroRNA-493-5p engineered exosomes delivered via piezoelectric microneedles for epigenetic modulation of macrophages in diabetic wound healing
Article Snippet: Proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes (Millipore, #ISEQ00010). .. After blocking with 5% skim milk, membranes were incubated overnight at 4 °C with primary antibodies against: Arg1 (Proteintech, #16001-1-AP), iNOS (Proteintech, #18985-1-AP), IL-1β (Proteintech, #16806-1-AP), PKM2 (ABclonal, #A19053), SLC7A11 (ABclonal, # A24146 ), HIF-1α (ABclonal, #A11945), and β-actin (Cell Signaling Technology, #4970). ..

Immunocytochemistry:

Article Title: Bacteria-responsive DNAgel system for targeted delivery of photothermally enhanced MXene/MoS 2 in the treatment of pyogenic osteomyelitis
Article Snippet: The PrimeScript RT reagent Kit with gDNA Eraser was purchased from Takara (Japan), and SYBR Green PCR Master Mix was purchased from Monad (China). .. Primary antibodies for immunocytochemistry and immunofluorescence staining—including ARG1 and iNOS, TRAP, β-Catenin, OCN and WNT4—were obtained from Proteintech (China). .. The IL-6, SP7, BMP2, and COL1A1 antibody was purchased from Abcam (UK).

Staining:

Article Title: Bacteria-responsive DNAgel system for targeted delivery of photothermally enhanced MXene/MoS 2 in the treatment of pyogenic osteomyelitis
Article Snippet: The PrimeScript RT reagent Kit with gDNA Eraser was purchased from Takara (Japan), and SYBR Green PCR Master Mix was purchased from Monad (China). .. Primary antibodies for immunocytochemistry and immunofluorescence staining—including ARG1 and iNOS, TRAP, β-Catenin, OCN and WNT4—were obtained from Proteintech (China). .. The IL-6, SP7, BMP2, and COL1A1 antibody was purchased from Abcam (UK).



Similar Products

95
ABclonal Biotechnology anti arg 1
Anti Arg 1, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/arg1/ARG1+Rabbit+mAb/pmc13597281-86-20-21
Average 95 stars, based on 1 article reviews
anti arg 1 - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier

95
ABclonal Biotechnology arg 1
Synthesis and Characterization of CEC NPs. (A) Diagram of the preparation process of CEC NPs. (B) FCM analysis shows the proportion of CC-linker-FITC modified EC NPs after co-incubation with different concentrations of CC-linker-FITC (10, 50, and 100 μM) with EC. (C) Representative CLSM images of CEC NPs showing signals for EC NPs (red, labeled with Dil) and CC-linker (green, labeled with FITC). Scale bar = 1 μm. (D) Representative TEM images of M2 Exo, EC NPs, and CEC NPs. Scale bar = 100 nm. (E) Western blot analysis of exosome markers (TSG101, CD81), and macrophage markers <t>(Arg-1)</t> in different material groups (M2 Exo, EC NPs, and CEC NPs), n = 3. (F) Zeta potential of CC-linker, EC NPs, and CEC NPs. (G) Hydrodynamic size distribution of Exo, EC NPs, and CEC NPs measured by DLS. (H) UV-Vis absorption spectra of CC-linker, EC NPs, and CEC NPs. (I) Hydrodynamic size of CEC NPs dispersed in PBS for different storage periods. (J) Cumulative release of CC-90011 from CEC NPs are measured in buffers with different pH values (Red: pH 6.5, Blue: pH 7.4). n = 3. All the representative images are shown from three independent experiments.
Arg 1, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/arg1/ARG1+Rabbit+mAb/pmc13355431-340-40-43
Average 95 stars, based on 1 article reviews
arg 1 - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier

96
Proteintech arg 1
Synthesis and Characterization of CEC NPs. (A) Diagram of the preparation process of CEC NPs. (B) FCM analysis shows the proportion of CC-linker-FITC modified EC NPs after co-incubation with different concentrations of CC-linker-FITC (10, 50, and 100 μM) with EC. (C) Representative CLSM images of CEC NPs showing signals for EC NPs (red, labeled with Dil) and CC-linker (green, labeled with FITC). Scale bar = 1 μm. (D) Representative TEM images of M2 Exo, EC NPs, and CEC NPs. Scale bar = 100 nm. (E) Western blot analysis of exosome markers (TSG101, CD81), and macrophage markers <t>(Arg-1)</t> in different material groups (M2 Exo, EC NPs, and CEC NPs), n = 3. (F) Zeta potential of CC-linker, EC NPs, and CEC NPs. (G) Hydrodynamic size distribution of Exo, EC NPs, and CEC NPs measured by DLS. (H) UV-Vis absorption spectra of CC-linker, EC NPs, and CEC NPs. (I) Hydrodynamic size of CEC NPs dispersed in PBS for different storage periods. (J) Cumulative release of CC-90011 from CEC NPs are measured in buffers with different pH values (Red: pH 6.5, Blue: pH 7.4). n = 3. All the representative images are shown from three independent experiments.
Arg 1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/arg1/ARG1+Antibody/pm42822644-107-21-23
Average 96 stars, based on 1 article reviews
arg 1 - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier

93
Boster Bio anti arg1
Synthesis and Characterization of CEC NPs. (A) Diagram of the preparation process of CEC NPs. (B) FCM analysis shows the proportion of CC-linker-FITC modified EC NPs after co-incubation with different concentrations of CC-linker-FITC (10, 50, and 100 μM) with EC. (C) Representative CLSM images of CEC NPs showing signals for EC NPs (red, labeled with Dil) and CC-linker (green, labeled with FITC). Scale bar = 1 μm. (D) Representative TEM images of M2 Exo, EC NPs, and CEC NPs. Scale bar = 100 nm. (E) Western blot analysis of exosome markers (TSG101, CD81), and macrophage markers <t>(Arg-1)</t> in different material groups (M2 Exo, EC NPs, and CEC NPs), n = 3. (F) Zeta potential of CC-linker, EC NPs, and CEC NPs. (G) Hydrodynamic size distribution of Exo, EC NPs, and CEC NPs measured by DLS. (H) UV-Vis absorption spectra of CC-linker, EC NPs, and CEC NPs. (I) Hydrodynamic size of CEC NPs dispersed in PBS for different storage periods. (J) Cumulative release of CC-90011 from CEC NPs are measured in buffers with different pH values (Red: pH 6.5, Blue: pH 7.4). n = 3. All the representative images are shown from three independent experiments.
Anti Arg1, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/arg1/Anti-liver+Arginase%2FARG1+Antibody+Picoband/pm42805596-126-77-80
Average 93 stars, based on 1 article reviews
anti arg1 - by Bioz Stars, 2026-10
93/100 stars
  Buy from Supplier

96
Proteintech antibodies against arg1
Synthesis and Characterization of CEC NPs. (A) Diagram of the preparation process of CEC NPs. (B) FCM analysis shows the proportion of CC-linker-FITC modified EC NPs after co-incubation with different concentrations of CC-linker-FITC (10, 50, and 100 μM) with EC. (C) Representative CLSM images of CEC NPs showing signals for EC NPs (red, labeled with Dil) and CC-linker (green, labeled with FITC). Scale bar = 1 μm. (D) Representative TEM images of M2 Exo, EC NPs, and CEC NPs. Scale bar = 100 nm. (E) Western blot analysis of exosome markers (TSG101, CD81), and macrophage markers <t>(Arg-1)</t> in different material groups (M2 Exo, EC NPs, and CEC NPs), n = 3. (F) Zeta potential of CC-linker, EC NPs, and CEC NPs. (G) Hydrodynamic size distribution of Exo, EC NPs, and CEC NPs measured by DLS. (H) UV-Vis absorption spectra of CC-linker, EC NPs, and CEC NPs. (I) Hydrodynamic size of CEC NPs dispersed in PBS for different storage periods. (J) Cumulative release of CC-90011 from CEC NPs are measured in buffers with different pH values (Red: pH 6.5, Blue: pH 7.4). n = 3. All the representative images are shown from three independent experiments.
Antibodies Against Arg1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/arg1/ARG1+Antibody/pm42800030-383-22-26
Average 96 stars, based on 1 article reviews
antibodies against arg1 - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier

95
ABclonal Biotechnology arg1
GA promotes M2 polarization via PRKCD to alleviate SI-ALI. (A) Immunohistochemistry analysis of PRKCD expression. (B) Hematoxylin and eosin staining of lung tissues. (C) Assessment of pulmonary edema by W/D ratio. (D) Immunofluorescence analysis of iNOS and <t>Arg1</t> expression in lung tissues. (E) ELISA analysis of IL-1β and TNF-α levels in BALF. Data are presented as mean ± SD (n=6 mice per group). For multiple-group comparisons, one-way ANOVA followed by Tukey’s post hoc test was applied. *, P<0.05; ***, P<0.001; ****, P<0.0001. ANOVA, analysis of variance; BALF, bronchoalveolar lavage fluid; DAPI, 4',6-diamidino-2-phenylindole; ELISA, enzyme-linked immunosorbent assay; GA, glycyrrhizic acid; IL-1β, interleukin-1 beta; NC, negative control; PRKCD, protein kinase C delta; SD, standard deviation; SI-ALI, sepsis-induced acute lung injury; TNF-α, tumor necrosis factor-alpha; W/D, wet-to-dry weight.
Arg1, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/arg1/ARG1+%2F+Arginase+1+Rabbit+pAb/pmc13656050-94-7-14
Average 95 stars, based on 1 article reviews
arg1 - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier

96
Proteintech anti arg 1
GA promotes M2 polarization via PRKCD to alleviate SI-ALI. (A) Immunohistochemistry analysis of PRKCD expression. (B) Hematoxylin and eosin staining of lung tissues. (C) Assessment of pulmonary edema by W/D ratio. (D) Immunofluorescence analysis of iNOS and <t>Arg1</t> expression in lung tissues. (E) ELISA analysis of IL-1β and TNF-α levels in BALF. Data are presented as mean ± SD (n=6 mice per group). For multiple-group comparisons, one-way ANOVA followed by Tukey’s post hoc test was applied. *, P<0.05; ***, P<0.001; ****, P<0.0001. ANOVA, analysis of variance; BALF, bronchoalveolar lavage fluid; DAPI, 4',6-diamidino-2-phenylindole; ELISA, enzyme-linked immunosorbent assay; GA, glycyrrhizic acid; IL-1β, interleukin-1 beta; NC, negative control; PRKCD, protein kinase C delta; SD, standard deviation; SI-ALI, sepsis-induced acute lung injury; TNF-α, tumor necrosis factor-alpha; W/D, wet-to-dry weight.
Anti Arg 1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/arg1/ARG1+Antibody/pm42775811-424-80-83
Average 96 stars, based on 1 article reviews
anti arg 1 - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier

96
Proteintech arg1
GA promotes M2 polarization via PRKCD to alleviate SI-ALI. (A) Immunohistochemistry analysis of PRKCD expression. (B) Hematoxylin and eosin staining of lung tissues. (C) Assessment of pulmonary edema by W/D ratio. (D) Immunofluorescence analysis of iNOS and <t>Arg1</t> expression in lung tissues. (E) ELISA analysis of IL-1β and TNF-α levels in BALF. Data are presented as mean ± SD (n=6 mice per group). For multiple-group comparisons, one-way ANOVA followed by Tukey’s post hoc test was applied. *, P<0.05; ***, P<0.001; ****, P<0.0001. ANOVA, analysis of variance; BALF, bronchoalveolar lavage fluid; DAPI, 4',6-diamidino-2-phenylindole; ELISA, enzyme-linked immunosorbent assay; GA, glycyrrhizic acid; IL-1β, interleukin-1 beta; NC, negative control; PRKCD, protein kinase C delta; SD, standard deviation; SI-ALI, sepsis-induced acute lung injury; TNF-α, tumor necrosis factor-alpha; W/D, wet-to-dry weight.
Arg1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/arg1/ARG1+Antibody/pm42772039-96-22-23
Average 96 stars, based on 1 article reviews
arg1 - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier

96
Proteintech arginase 1
GA promotes M2 polarization via PRKCD to alleviate SI-ALI. (A) Immunohistochemistry analysis of PRKCD expression. (B) Hematoxylin and eosin staining of lung tissues. (C) Assessment of pulmonary edema by W/D ratio. (D) Immunofluorescence analysis of iNOS and <t>Arg1</t> expression in lung tissues. (E) ELISA analysis of IL-1β and TNF-α levels in BALF. Data are presented as mean ± SD (n=6 mice per group). For multiple-group comparisons, one-way ANOVA followed by Tukey’s post hoc test was applied. *, P<0.05; ***, P<0.001; ****, P<0.0001. ANOVA, analysis of variance; BALF, bronchoalveolar lavage fluid; DAPI, 4',6-diamidino-2-phenylindole; ELISA, enzyme-linked immunosorbent assay; GA, glycyrrhizic acid; IL-1β, interleukin-1 beta; NC, negative control; PRKCD, protein kinase C delta; SD, standard deviation; SI-ALI, sepsis-induced acute lung injury; TNF-α, tumor necrosis factor-alpha; W/D, wet-to-dry weight.
Arginase 1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/arg1/ARG1+Antibody/pm42532044-526-48-53
Average 96 stars, based on 1 article reviews
arginase 1 - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier

95
ABclonal Biotechnology anti arg1
GA promotes M2 polarization via PRKCD to alleviate SI-ALI. (A) Immunohistochemistry analysis of PRKCD expression. (B) Hematoxylin and eosin staining of lung tissues. (C) Assessment of pulmonary edema by W/D ratio. (D) Immunofluorescence analysis of iNOS and <t>Arg1</t> expression in lung tissues. (E) ELISA analysis of IL-1β and TNF-α levels in BALF. Data are presented as mean ± SD (n=6 mice per group). For multiple-group comparisons, one-way ANOVA followed by Tukey’s post hoc test was applied. *, P<0.05; ***, P<0.001; ****, P<0.0001. ANOVA, analysis of variance; BALF, bronchoalveolar lavage fluid; DAPI, 4',6-diamidino-2-phenylindole; ELISA, enzyme-linked immunosorbent assay; GA, glycyrrhizic acid; IL-1β, interleukin-1 beta; NC, negative control; PRKCD, protein kinase C delta; SD, standard deviation; SI-ALI, sepsis-induced acute lung injury; TNF-α, tumor necrosis factor-alpha; W/D, wet-to-dry weight.
Anti Arg1, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/arg1/ARG1+%2F+Arginase+1+Rabbit+pAb/10__31083_slash_fbl53591-84-23-26
Average 95 stars, based on 1 article reviews
anti arg1 - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier

Image Search Results


Synthesis and Characterization of CEC NPs. (A) Diagram of the preparation process of CEC NPs. (B) FCM analysis shows the proportion of CC-linker-FITC modified EC NPs after co-incubation with different concentrations of CC-linker-FITC (10, 50, and 100 μM) with EC. (C) Representative CLSM images of CEC NPs showing signals for EC NPs (red, labeled with Dil) and CC-linker (green, labeled with FITC). Scale bar = 1 μm. (D) Representative TEM images of M2 Exo, EC NPs, and CEC NPs. Scale bar = 100 nm. (E) Western blot analysis of exosome markers (TSG101, CD81), and macrophage markers (Arg-1) in different material groups (M2 Exo, EC NPs, and CEC NPs), n = 3. (F) Zeta potential of CC-linker, EC NPs, and CEC NPs. (G) Hydrodynamic size distribution of Exo, EC NPs, and CEC NPs measured by DLS. (H) UV-Vis absorption spectra of CC-linker, EC NPs, and CEC NPs. (I) Hydrodynamic size of CEC NPs dispersed in PBS for different storage periods. (J) Cumulative release of CC-90011 from CEC NPs are measured in buffers with different pH values (Red: pH 6.5, Blue: pH 7.4). n = 3. All the representative images are shown from three independent experiments.

Journal: Bioactive Materials

Article Title: Biomimetic M2 exosome-based dual-drug nanoplatform for combined immunomodulation and bone protection in rheumatoid arthritis

doi: 10.1016/j.bioactmat.2026.05.010

Figure Lengend Snippet: Synthesis and Characterization of CEC NPs. (A) Diagram of the preparation process of CEC NPs. (B) FCM analysis shows the proportion of CC-linker-FITC modified EC NPs after co-incubation with different concentrations of CC-linker-FITC (10, 50, and 100 μM) with EC. (C) Representative CLSM images of CEC NPs showing signals for EC NPs (red, labeled with Dil) and CC-linker (green, labeled with FITC). Scale bar = 1 μm. (D) Representative TEM images of M2 Exo, EC NPs, and CEC NPs. Scale bar = 100 nm. (E) Western blot analysis of exosome markers (TSG101, CD81), and macrophage markers (Arg-1) in different material groups (M2 Exo, EC NPs, and CEC NPs), n = 3. (F) Zeta potential of CC-linker, EC NPs, and CEC NPs. (G) Hydrodynamic size distribution of Exo, EC NPs, and CEC NPs measured by DLS. (H) UV-Vis absorption spectra of CC-linker, EC NPs, and CEC NPs. (I) Hydrodynamic size of CEC NPs dispersed in PBS for different storage periods. (J) Cumulative release of CC-90011 from CEC NPs are measured in buffers with different pH values (Red: pH 6.5, Blue: pH 7.4). n = 3. All the representative images are shown from three independent experiments.

Article Snippet: For immunofluorescence staining, RAW264.7 cells were seeded into a well plate, and after LPS stimulation for 12 h, M2 Exo, EC, and CEC were added for treatment for 24 h. Subsequently, the expression of iNOS (1:200, A3774, ABclonal, China) and Arg-1 (1:200, A4923, ABclonal, China) in macrophages was detected using CLSM.

Techniques: Modification, Incubation, Labeling, Western Blot, Zeta Potential Analyzer

M1-to-M2 repolarization by EC NPs in vitro . (A-B) Representative CLSM images of the polarization markers of macrophages iNOS (green) and Arg-1 (red) after treatment with CXB, Exo, and EC NPs. DAPI: blue. Scale bar = 25 μm. (C-D) RT-qPCR (C) and FCM (D) verification of the expression of Arg-1, iNOS, CD86, CD206, IL-1β, IL-6, and IL-10 in macrophages after different treatment. (E) Quantification of the CD206 + /CD86 + ratio in RAW 264.7 macrophages corresponding to the FCM data in (D). (F) Concentrations of IL-6 and IL-10 in cell culture supernatants were measured by ELISA. (G-J) The representative CLSM images (G) and FCM (J) results of ROS (green) generation in M1 macrophages treated with different conditions (CXB, Exo, EC NPs) detected using the DCFH-DA probe. Quantitative analysis of the fluorescence intensity of DCFH-DA in the CLSM images (H) and in FCM (J), corresponding to (G) and (I), respectively. n = 3, Scale bar = 25 μm ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001, ns: no significance. All the representative images are shown from three independent experiments.

Journal: Bioactive Materials

Article Title: Biomimetic M2 exosome-based dual-drug nanoplatform for combined immunomodulation and bone protection in rheumatoid arthritis

doi: 10.1016/j.bioactmat.2026.05.010

Figure Lengend Snippet: M1-to-M2 repolarization by EC NPs in vitro . (A-B) Representative CLSM images of the polarization markers of macrophages iNOS (green) and Arg-1 (red) after treatment with CXB, Exo, and EC NPs. DAPI: blue. Scale bar = 25 μm. (C-D) RT-qPCR (C) and FCM (D) verification of the expression of Arg-1, iNOS, CD86, CD206, IL-1β, IL-6, and IL-10 in macrophages after different treatment. (E) Quantification of the CD206 + /CD86 + ratio in RAW 264.7 macrophages corresponding to the FCM data in (D). (F) Concentrations of IL-6 and IL-10 in cell culture supernatants were measured by ELISA. (G-J) The representative CLSM images (G) and FCM (J) results of ROS (green) generation in M1 macrophages treated with different conditions (CXB, Exo, EC NPs) detected using the DCFH-DA probe. Quantitative analysis of the fluorescence intensity of DCFH-DA in the CLSM images (H) and in FCM (J), corresponding to (G) and (I), respectively. n = 3, Scale bar = 25 μm ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001, ns: no significance. All the representative images are shown from three independent experiments.

Article Snippet: For immunofluorescence staining, RAW264.7 cells were seeded into a well plate, and after LPS stimulation for 12 h, M2 Exo, EC, and CEC were added for treatment for 24 h. Subsequently, the expression of iNOS (1:200, A3774, ABclonal, China) and Arg-1 (1:200, A4923, ABclonal, China) in macrophages was detected using CLSM.

Techniques: In Vitro, Quantitative RT-PCR, Expressing, Cell Culture, Enzyme-linked Immunosorbent Assay, Fluorescence

Histological analysis of therapeutic effects following CEC administration in vivo . (A) Histopathological evaluation of ankle joints in each group was performed by H&E staining. Scale bar = 200 μm. (B) Histochemical identification of OCs by TRAP staining in arthritic joints from different treatment groups. Scale bar = 200 μm. (C) Cartilage damage in murine ankle joints following different treatments was assessed by SOFG staining. Scale bar = 50 μm. (D) Immunofluorescence staining was performed to detect the expression of macrophage marker F4/80 (purple), M1 macrophage marker (iNOS, red) and M2 macrophage marker (Arg-1, green) in joint tissues of healthy mice and CIA mice receiving different treatments, scale bar = 100 μm. All images are representative of five mice per group.

Journal: Bioactive Materials

Article Title: Biomimetic M2 exosome-based dual-drug nanoplatform for combined immunomodulation and bone protection in rheumatoid arthritis

doi: 10.1016/j.bioactmat.2026.05.010

Figure Lengend Snippet: Histological analysis of therapeutic effects following CEC administration in vivo . (A) Histopathological evaluation of ankle joints in each group was performed by H&E staining. Scale bar = 200 μm. (B) Histochemical identification of OCs by TRAP staining in arthritic joints from different treatment groups. Scale bar = 200 μm. (C) Cartilage damage in murine ankle joints following different treatments was assessed by SOFG staining. Scale bar = 50 μm. (D) Immunofluorescence staining was performed to detect the expression of macrophage marker F4/80 (purple), M1 macrophage marker (iNOS, red) and M2 macrophage marker (Arg-1, green) in joint tissues of healthy mice and CIA mice receiving different treatments, scale bar = 100 μm. All images are representative of five mice per group.

Article Snippet: For immunofluorescence staining, RAW264.7 cells were seeded into a well plate, and after LPS stimulation for 12 h, M2 Exo, EC, and CEC were added for treatment for 24 h. Subsequently, the expression of iNOS (1:200, A3774, ABclonal, China) and Arg-1 (1:200, A4923, ABclonal, China) in macrophages was detected using CLSM.

Techniques: In Vivo, Staining, Immunofluorescence, Expressing, Marker

GA promotes M2 polarization via PRKCD to alleviate SI-ALI. (A) Immunohistochemistry analysis of PRKCD expression. (B) Hematoxylin and eosin staining of lung tissues. (C) Assessment of pulmonary edema by W/D ratio. (D) Immunofluorescence analysis of iNOS and Arg1 expression in lung tissues. (E) ELISA analysis of IL-1β and TNF-α levels in BALF. Data are presented as mean ± SD (n=6 mice per group). For multiple-group comparisons, one-way ANOVA followed by Tukey’s post hoc test was applied. *, P<0.05; ***, P<0.001; ****, P<0.0001. ANOVA, analysis of variance; BALF, bronchoalveolar lavage fluid; DAPI, 4',6-diamidino-2-phenylindole; ELISA, enzyme-linked immunosorbent assay; GA, glycyrrhizic acid; IL-1β, interleukin-1 beta; NC, negative control; PRKCD, protein kinase C delta; SD, standard deviation; SI-ALI, sepsis-induced acute lung injury; TNF-α, tumor necrosis factor-alpha; W/D, wet-to-dry weight.

Journal: Journal of Thoracic Disease

Article Title: Glycyrrhizic acid attenuates sepsis-induced acute lung injury by regulating macrophage M2 polarization via PRKCD

doi: 10.21037/jtd-2026-1327

Figure Lengend Snippet: GA promotes M2 polarization via PRKCD to alleviate SI-ALI. (A) Immunohistochemistry analysis of PRKCD expression. (B) Hematoxylin and eosin staining of lung tissues. (C) Assessment of pulmonary edema by W/D ratio. (D) Immunofluorescence analysis of iNOS and Arg1 expression in lung tissues. (E) ELISA analysis of IL-1β and TNF-α levels in BALF. Data are presented as mean ± SD (n=6 mice per group). For multiple-group comparisons, one-way ANOVA followed by Tukey’s post hoc test was applied. *, P<0.05; ***, P<0.001; ****, P<0.0001. ANOVA, analysis of variance; BALF, bronchoalveolar lavage fluid; DAPI, 4',6-diamidino-2-phenylindole; ELISA, enzyme-linked immunosorbent assay; GA, glycyrrhizic acid; IL-1β, interleukin-1 beta; NC, negative control; PRKCD, protein kinase C delta; SD, standard deviation; SI-ALI, sepsis-induced acute lung injury; TNF-α, tumor necrosis factor-alpha; W/D, wet-to-dry weight.

Article Snippet: Tissue sections were incubated with iNOS and Arg1 along with macrophage marker F4/80 (A18637, ABclonal).

Techniques: Immunohistochemistry, Analysis, Expressing, H&E Stain, Immunofluorescence, Enzyme-linked Immunosorbent Assay, Negative Control, Standard Deviation