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apy0201  (MedChemExpress)


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    MedChemExpress apy0201
    Apy0201, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 967 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/apy0201/product/MedChemExpress
    Average 99 stars, based on 967 article reviews
    apy0201 - by Bioz Stars, 2026-02
    99/100 stars

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    (A to D) Vero-E6 cells were pretreated with the indicated concentration of Cathepsin L inhibitor Calpain Inhibitor III (A), PIKfyve inhibitor <t>APY0201</t> (B) SMARCA4 inhibitor PFI-3 (C), SMAD3 inhibitor SIS3 (D) for 48 hours and then infected with SARS-CoV-2 at a MOI of 0.2. Cell viability was measured at 3dpi and compared to mock infected controls. Red, infected; blue, mock (E to F) Vero-E6 cells were pretreated with 10 μM Calpain inhibitor III, PFI-3 or SIS3 for 48 hours and then infected with icSARS-CoV-2 mNG at a MOI of 1. Infected cell frequencies were measured by mNeonGreen expression at 2 dpi. Data were analyzed by one-way ANOVA with Tukey’s multiple comparison test. Shown are means ± SEM. ns, not statistically significant; *P < 0.05; **P < 0.01; ***P < 0.001. Data are pooled from two independent experiments each in duplicate except in (E), where results are shown from one representative experiment.
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    (A to D) Vero-E6 cells were pretreated with the indicated concentration of Cathepsin L inhibitor Calpain Inhibitor III (A), PIKfyve inhibitor APY0201 (B) SMARCA4 inhibitor PFI-3 (C), SMAD3 inhibitor SIS3 (D) for 48 hours and then infected with SARS-CoV-2 at a MOI of 0.2. Cell viability was measured at 3dpi and compared to mock infected controls. Red, infected; blue, mock (E to F) Vero-E6 cells were pretreated with 10 μM Calpain inhibitor III, PFI-3 or SIS3 for 48 hours and then infected with icSARS-CoV-2 mNG at a MOI of 1. Infected cell frequencies were measured by mNeonGreen expression at 2 dpi. Data were analyzed by one-way ANOVA with Tukey’s multiple comparison test. Shown are means ± SEM. ns, not statistically significant; *P < 0.05; **P < 0.01; ***P < 0.001. Data are pooled from two independent experiments each in duplicate except in (E), where results are shown from one representative experiment.

    Journal: bioRxiv

    Article Title: Genome-wide CRISPR screen reveals host genes that regulate SARS-CoV-2 infection

    doi: 10.1101/2020.06.16.155101

    Figure Lengend Snippet: (A to D) Vero-E6 cells were pretreated with the indicated concentration of Cathepsin L inhibitor Calpain Inhibitor III (A), PIKfyve inhibitor APY0201 (B) SMARCA4 inhibitor PFI-3 (C), SMAD3 inhibitor SIS3 (D) for 48 hours and then infected with SARS-CoV-2 at a MOI of 0.2. Cell viability was measured at 3dpi and compared to mock infected controls. Red, infected; blue, mock (E to F) Vero-E6 cells were pretreated with 10 μM Calpain inhibitor III, PFI-3 or SIS3 for 48 hours and then infected with icSARS-CoV-2 mNG at a MOI of 1. Infected cell frequencies were measured by mNeonGreen expression at 2 dpi. Data were analyzed by one-way ANOVA with Tukey’s multiple comparison test. Shown are means ± SEM. ns, not statistically significant; *P < 0.05; **P < 0.01; ***P < 0.001. Data are pooled from two independent experiments each in duplicate except in (E), where results are shown from one representative experiment.

    Article Snippet: Calpain Inhibitor III (#14283), SIS3 (#15495), APY0201 (#9001589), and PFI-3 (#15267) were purchased from Cayman Chemical.

    Techniques: Concentration Assay, Infection, Expressing