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Protein levels of key cGAS-STING effector proteins are affected by <t>APTX</t> KO during dsDNA stimulation. Microglia cells were transfected with dsDNA and harvested for western blot analysis. Blots were stained for STING and pSTING (A) and levels quantified in (B,C) , TBK1 and pTBK1 (D) and levels quantified in (E,F) and <t>for</t> <t>IRF3</t> and pIRF3 (G) quantified in (H,I) . Graphs show mean ± SEM, analyzed by two-way ANOVA, where significance is scored as ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001.
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Protein levels of key cGAS-STING effector proteins are affected by <t>APTX</t> KO during dsDNA stimulation. Microglia cells were transfected with dsDNA and harvested for western blot analysis. Blots were stained for STING and pSTING (A) and levels quantified in (B,C) , TBK1 and pTBK1 (D) and levels quantified in (E,F) and <t>for</t> <t>IRF3</t> and pIRF3 (G) quantified in (H,I) . Graphs show mean ± SEM, analyzed by two-way ANOVA, where significance is scored as ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001.
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Protein levels of key cGAS-STING effector proteins are affected by APTX KO during dsDNA stimulation. Microglia cells were transfected with dsDNA and harvested for western blot analysis. Blots were stained for STING and pSTING (A) and levels quantified in (B,C) , TBK1 and pTBK1 (D) and levels quantified in (E,F) and for IRF3 and pIRF3 (G) quantified in (H,I) . Graphs show mean ± SEM, analyzed by two-way ANOVA, where significance is scored as ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: Frontiers in Aging Neuroscience

Article Title: The DNA repair enzyme, aprataxin, plays a role in innate immune signaling

doi: 10.3389/fnagi.2023.1290681

Figure Lengend Snippet: Protein levels of key cGAS-STING effector proteins are affected by APTX KO during dsDNA stimulation. Microglia cells were transfected with dsDNA and harvested for western blot analysis. Blots were stained for STING and pSTING (A) and levels quantified in (B,C) , TBK1 and pTBK1 (D) and levels quantified in (E,F) and for IRF3 and pIRF3 (G) quantified in (H,I) . Graphs show mean ± SEM, analyzed by two-way ANOVA, where significance is scored as ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: APTX (sc-374108), IRF3 (SL-12, sc-33641) and Rig1 (D12, sc-376845) antibodies were from Santa Cruz and Actin (A5441) was from Merck.

Techniques: Transfection, Western Blot, Staining