Journal: Clinical Cancer Research

Article Title: Aprataxin Tumor Levels Predict Response of Colorectal Cancer Patients to Irinotecan-based Treatment

doi: 10.1158/1078-0432.ccr-09-3275

Figure Lengend Snippet: Fig. 1. Correlation between aprataxin (APTX) levels and camptothecin sensitivity. A, there was a negative correlation between mRNA expression levels in a panel of 30 colorectal cancer cell lines and the induction of apoptosis in response to treatment with 1 μmol/L camptothecin for 72 h. B, individual levels of aprataxin and the apoptotic response in these 30 cell lines.

Article Snippet: An aprataxin rabbit polyclonal antibody raised against the COOHterminus of human aprataxin was used at a 1:100 dilution (4°C overnight; Aviva Systems Biology Corp).

Techniques: Expressing

Journal: Clinical Cancer Research

Article Title: Aprataxin Tumor Levels Predict Response of Colorectal Cancer Patients to Irinotecan-based Treatment

doi: 10.1158/1078-0432.ccr-09-3275

Figure Lengend Snippet: Fig. 3. Aprataxin tumor levels and survival of patients with advanced colorectal cancer receiving irinotecan-based treatment. Immunohistochemical staining of colorectal tumors showed a gradient of expression, with some tumors having no detectable aprataxin (A), high levels (D), or intermediate levels of expression (B and C). Progression-free (E) and overall survival (F) according to aprataxin protein levels are shown (Kaplan-Meier plots). Log-rank P values are shown. PFS, progression-free survival; OS, overall survival; HR, hazard ratio; 95% CI, 95% confidence interval.

Article Snippet: An aprataxin rabbit polyclonal antibody raised against the COOHterminus of human aprataxin was used at a 1:100 dilution (4°C overnight; Aviva Systems Biology Corp).

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: PLoS ONE

Article Title: Synergistic Anticancer Effects of Polyphyllin I and Evodiamine on Freshly-Removed Human Gastric Tumors

doi: 10.1371/journal.pone.0065164

Figure Lengend Snippet: A, The mRNA expression levels of ERCC1,TS,TOPO1 and APTX. The lines in the middle stood for mean with SEM; n = 40. B, correlation between ERCC1 mRNA expression levels and Pt sensitivity (rho = −0.645, P <0.001, n = 40). C, correlation between TS mRNA expression levels and 5-FU sensitivity (rho = −0.803, P <0.001, n = 40). D, correlation between APTX mRNA expression levels and EVO sensitivity (rho = −0.322, P = 0.036, n = 40).

Article Snippet: The Assay IDs (Applied Biosystems, Foster City, CA) for the primers and probes were as follows: ACTB: Hs99999903_m1, APTX: Hs00214452_m1, ERCC1: Hs00157415_m1, TS: Hs00426586_m1, TOPO1: Hs00243257_m1.

Techniques: Expressing

Journal: The Journal of Biological Chemistry

Article Title: The scaffold protein XRCC1 stabilizes the formation of polβ/gap DNA and ligase IIIα/nick DNA complexes in base excision repair

doi: 10.1016/j.jbc.2021.101025

Figure Lengend Snippet: Protein–protein interaction analyses between XRCC1 and BER proteins. The real-time protein–protein interaction kinetics of wild-type XRCC1 with polβ ( A ), ligase IIIα ( B ), and APTX ( C ) is measured by SPR assay where the interacting protein partner of XRCC1 was immobilized on CM5 biosensors. The ligand association and dissociation phases are shown for the protein concentration range of XRCC1 on the side of sensorgrams. D , table shows comparison of the equilibrium binding constants (K D ) between XRCC1 wild-type and variants for polβ, ligase IIIα, and APTX. The protein–protein interaction kinetics for XRCC1 variants are presented in Figs. S3–S5 .

Article Snippet: Human wild-type full-length APTX gene was obtained from GenScript and cloned into pET-24b expression vector (Novagen).

Techniques: SPR Assay, Protein Concentration, Comparison, Binding Assay