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anti aplnr  (Proteintech)


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    Structured Review

    Proteintech anti aplnr
    Anti Aplnr, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/apj/APJ+Antibody/10__1096_slash_fj__202502186rr-52-17-19
    Average 93 stars, based on 58 article reviews
    anti aplnr - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Apelin-13 alleviates intrauterine adhesion by inhibiting epithelial-mesenchymal transition of endometrial epithelial cells and promoting angiogenesis.
    Article Snippet: Intrauterine adhesion (IUA) is a common complication of surgical manipulation of the uterine cavity such as abortion.. The pathology of IUA is characterized by fibrosis, but the pathogenesis is not fully understood.. The function of Apelin-13 in IUA and related mechanisms were investigated in this study.

    Article Title: Apelin-13/APJ promotes neural stem cells to repair ischemic stroke.
    Article Snippet: Mixed with loading buffer, the protein samples were then kept at 70 ◦C for 10 min. Equal-sized protein samples were separated by 4–12 % ACE Future PAGE and were then transferred to a 0.2-μm polyvinylidene fluoride (PVDF) membrane (Millipore, USA). .. Then, the membranes were incubated at room temperature in Protein Free Rapid Blocking Buffer (1 ×) (Epizyme Biotech, China) for 1 h. The membrane was subsequently incubated with anti-rabbit primary antibodies at 4 ◦C overnight: Bcl-2 (1:500; #26593-1-AP; Proteintech, USA), Bax (1:2000; # 50599-2-Ig; Proteintech, USA), APJ (1:1000; #20341-1-AP; Proteintech, USA), BDNF (1:1000; #108319; Abcam, USA), , β-actin (1:1000; #3700; Cell Signaling Technology, USA). .. On the second day, 1× TBST was used to wash the membranes three times for 10 min each, and after that, the membranes were incubated with anti-rabbit IgG and anti-mouse IgG, HRP-linked antibody (1:2000, Cell Signaling, USA) at room temperature for 1 h. After rinsing three times with 1× TBST for 10 min each, the bands treated with chemiluminescence reagents were detected by the ChemiDocTM Touch Imaging System (Bio-Rad, USA).

    Article Title: Aerobic Exercise Upregulates Renal‐Derived ELABELA to Improve Myocardial Fibrosis by Activating APJ‐AMPK‐Sirt1 and Inhibiting TGFβ1‐Smad2/3 Signaling Pathways in Myocardial Infarction Mice
    Article Snippet: ELABELA (ELA) has been identified as a potential cardiovascular protective factor.. However, the source of endogenous ELA and its molecular mechanism in myocardial fibrosis inhibition remain incompletely understood.. Herein, we found that aerobic exercise significantly improved renal apoptosis caused by MI, inhibited inflammation, attenuated structural damage, enhanced renal function, and increased expression and secretion levels of renal ELA.

    Article Title: The Elabela-APJ axis attenuates sepsis-induced myocardial dysfunction by reducing pyroptosis by balancing the formation and degradation of autophagosomes.
    Article Snippet: .. After blocking, the membranes were incubated overnight at 4 ◦C with antibodies including LC3-B (A5618, Abcam), GAPDH (10,494–1-AP, Proteintech), APJ (20341-1-AP, Proteintech), P62 (23214 S, CST), Atg-5 (12,994 T, CST), NLRP3 (A5652, Abcam), GSDMD-N (A24476, Abcam), IL-1β (A16288, Abcam), and IL-18 (A20473, Abcam). ..

    Blocking Assay:

    Article Title: Apelin-13/APJ promotes neural stem cells to repair ischemic stroke.
    Article Snippet: Mixed with loading buffer, the protein samples were then kept at 70 ◦C for 10 min. Equal-sized protein samples were separated by 4–12 % ACE Future PAGE and were then transferred to a 0.2-μm polyvinylidene fluoride (PVDF) membrane (Millipore, USA). .. Then, the membranes were incubated at room temperature in Protein Free Rapid Blocking Buffer (1 ×) (Epizyme Biotech, China) for 1 h. The membrane was subsequently incubated with anti-rabbit primary antibodies at 4 ◦C overnight: Bcl-2 (1:500; #26593-1-AP; Proteintech, USA), Bax (1:2000; # 50599-2-Ig; Proteintech, USA), APJ (1:1000; #20341-1-AP; Proteintech, USA), BDNF (1:1000; #108319; Abcam, USA), , β-actin (1:1000; #3700; Cell Signaling Technology, USA). .. On the second day, 1× TBST was used to wash the membranes three times for 10 min each, and after that, the membranes were incubated with anti-rabbit IgG and anti-mouse IgG, HRP-linked antibody (1:2000, Cell Signaling, USA) at room temperature for 1 h. After rinsing three times with 1× TBST for 10 min each, the bands treated with chemiluminescence reagents were detected by the ChemiDocTM Touch Imaging System (Bio-Rad, USA).

    Article Title: The Elabela-APJ axis attenuates sepsis-induced myocardial dysfunction by reducing pyroptosis by balancing the formation and degradation of autophagosomes.
    Article Snippet: .. After blocking, the membranes were incubated overnight at 4 ◦C with antibodies including LC3-B (A5618, Abcam), GAPDH (10,494–1-AP, Proteintech), APJ (20341-1-AP, Proteintech), P62 (23214 S, CST), Atg-5 (12,994 T, CST), NLRP3 (A5652, Abcam), GSDMD-N (A24476, Abcam), IL-1β (A16288, Abcam), and IL-18 (A20473, Abcam). ..

    Membrane:

    Article Title: Apelin-13/APJ promotes neural stem cells to repair ischemic stroke.
    Article Snippet: Mixed with loading buffer, the protein samples were then kept at 70 ◦C for 10 min. Equal-sized protein samples were separated by 4–12 % ACE Future PAGE and were then transferred to a 0.2-μm polyvinylidene fluoride (PVDF) membrane (Millipore, USA). .. Then, the membranes were incubated at room temperature in Protein Free Rapid Blocking Buffer (1 ×) (Epizyme Biotech, China) for 1 h. The membrane was subsequently incubated with anti-rabbit primary antibodies at 4 ◦C overnight: Bcl-2 (1:500; #26593-1-AP; Proteintech, USA), Bax (1:2000; # 50599-2-Ig; Proteintech, USA), APJ (1:1000; #20341-1-AP; Proteintech, USA), BDNF (1:1000; #108319; Abcam, USA), , β-actin (1:1000; #3700; Cell Signaling Technology, USA). .. On the second day, 1× TBST was used to wash the membranes three times for 10 min each, and after that, the membranes were incubated with anti-rabbit IgG and anti-mouse IgG, HRP-linked antibody (1:2000, Cell Signaling, USA) at room temperature for 1 h. After rinsing three times with 1× TBST for 10 min each, the bands treated with chemiluminescence reagents were detected by the ChemiDocTM Touch Imaging System (Bio-Rad, USA).



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