apaf1 (Proteintech)
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Proteintech
apaf1
Apaf1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 212 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/apaf1/IGF2BP3+Antibody/pm41787385-145-38-41
Average 96 stars, based on 212 article reviews
Apaf1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 212 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/apaf1/IGF2BP3+Antibody/pm41787385-145-38-41
Average 96 stars, based on 212 article reviews
apaf1 - by Bioz Stars,
2026-10
96/100 stars
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Incubation:Article Title: Oxymatrine Alleviates Cerebral Ischemia/Reperfusion Injury By Targeting HDAC1 to Regulate Mitochondria-Related Autophagy and Oxidative Stress. Article Snippet: Coronal sections underwent antigen retrieval using citrate buffer (Beyotime, Jiangsu, China), followed by blocking with 10% goat serum in PBS for 1 h at room temperature. .. Sections were incubated overnight at 4 °C with the following primary antibodies diluted in PBS:LC3 and PINK1 (1:200, ABclonal, Wuhan, China); Beclin1, Article Title: Oxymatrine Alleviates Cerebral Ischemia/Reperfusion Injury By Targeting HDAC1 to Regulate Mitochondria-Related Autophagy and Oxidative Stress Article Snippet: Coronal sections underwent antigen retrieval using citrate buffer (Beyotime, Jiangsu, China), followed by blocking with 10% goat serum in PBS for 1 h at room temperature. .. Sections were incubated overnight at 4 °C with the following primary antibodies diluted in PBS:LC3 and PINK1 (1:200, ABclonal, Wuhan, China); Beclin1, Article Title: EMC6 regulates acinar apoptosis via APAF1 in acute and chronic pancreatitis Article Snippet: Briefly, tissue homogenate was incubated with immunoprecipitation lysate (Servicebio) overnight at 4°C, followed by centrifuging for 10 min at 14,000 × g . After the supernatant was collected and the protein concentration was measured by BCA protein quantitative detection kit (G2026; Servicebio, China), a small amount of tissue lysate was collected for input experiments. .. The tissue lysate was added with 1.0 μg of rabbit IgG (Servicebio) and 20 μl of A /G beads (Millipore) and incubated at 4 °C for 1 h. After centrifugation, the supernatant was incubated with respective primary antibodies against EMC6 (Omnimabs; 2 μg), Western Blot:Article Title: EMC6 regulates acinar apoptosis via APAF1 in acute and chronic pancreatitis Article Snippet: .. Finally, Supernatant was analyzed by immunoblotting with EMC6 (Omnimabs; diluted 1:1000) and other:Article Title: LncRNA EGFR-AS1 inhibits ferroptosis to reduce radiosensitivity of cervical cancer through the m6A/IGF2BP3/APAF1 axis. Article Snippet: Western blot 294 Cervical cancer cells were lysed by RIPA buffer (Thermo), allowing 295 for the extraction of total protein. Centrifugation:Article Title: EMC6 regulates acinar apoptosis via APAF1 in acute and chronic pancreatitis Article Snippet: Briefly, tissue homogenate was incubated with immunoprecipitation lysate (Servicebio) overnight at 4°C, followed by centrifuging for 10 min at 14,000 × g . After the supernatant was collected and the protein concentration was measured by BCA protein quantitative detection kit (G2026; Servicebio, China), a small amount of tissue lysate was collected for input experiments. .. The tissue lysate was added with 1.0 μg of rabbit IgG (Servicebio) and 20 μl of A /G beads (Millipore) and incubated at 4 °C for 1 h. After centrifugation, the supernatant was incubated with respective primary antibodies against EMC6 (Omnimabs; 2 μg), Negative Control:Article Title: EMC6 regulates acinar apoptosis via APAF1 in acute and chronic pancreatitis Article Snippet: Briefly, tissue homogenate was incubated with immunoprecipitation lysate (Servicebio) overnight at 4°C, followed by centrifuging for 10 min at 14,000 × g . After the supernatant was collected and the protein concentration was measured by BCA protein quantitative detection kit (G2026; Servicebio, China), a small amount of tissue lysate was collected for input experiments. .. The tissue lysate was added with 1.0 μg of rabbit IgG (Servicebio) and 20 μl of A /G beads (Millipore) and incubated at 4 °C for 1 h. After centrifugation, the supernatant was incubated with respective primary antibodies against EMC6 (Omnimabs; 2 μg), |
