Review





Similar Products

akt inhibitor  (MedChemExpress)
95
MedChemExpress akt inhibitor
E2 and VitD3 improves osteogenesis by <t>PI3K/AKT/mTOR</t> pathway. (A) Q-PCR analysis of mRNA level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (B) The protein level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (C) The WB analysis of protein level of pan and phsopho-PI3K, pan and phospho-Akt, pan and phospho-mTOR, and pan and phospho-FOXO3 in MC3T3-E1 cells treated with PBS, E2, VitD3, E2 plus VitD3. (D) The MC3T3-E1 cells were pretreated with or <t>without</t> <t>Akti1/2</t> (5uM), and were induced to osteoblasts with PBS, E2, VitD3, E2+VitD3. The ALP staining was utilized to evaluate the osteogenic differentiation in each group.
Akt Inhibitor, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/akt inhibitor/product/MedChemExpress
Average 95 stars, based on 1 article reviews
akt inhibitor - by Bioz Stars, 2026-02
95/100 stars
  Buy from Supplier
anti akt  (Proteintech)
96
Proteintech anti akt
E2 and VitD3 improves osteogenesis by <t>PI3K/AKT/mTOR</t> pathway. (A) Q-PCR analysis of mRNA level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (B) The protein level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (C) The WB analysis of protein level of pan and phsopho-PI3K, pan and phospho-Akt, pan and phospho-mTOR, and pan and phospho-FOXO3 in MC3T3-E1 cells treated with PBS, E2, VitD3, E2 plus VitD3. (D) The MC3T3-E1 cells were pretreated with or <t>without</t> <t>Akti1/2</t> (5uM), and were induced to osteoblasts with PBS, E2, VitD3, E2+VitD3. The ALP staining was utilized to evaluate the osteogenic differentiation in each group.
Anti Akt, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti akt/product/Proteintech
Average 96 stars, based on 1 article reviews
anti akt - by Bioz Stars, 2026-02
96/100 stars
  Buy from Supplier
anti pakt  (Proteintech)
96
Proteintech anti pakt
E2 and VitD3 improves osteogenesis by <t>PI3K/AKT/mTOR</t> pathway. (A) Q-PCR analysis of mRNA level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (B) The protein level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (C) The WB analysis of protein level of pan and phsopho-PI3K, pan and phospho-Akt, pan and phospho-mTOR, and pan and phospho-FOXO3 in MC3T3-E1 cells treated with PBS, E2, VitD3, E2 plus VitD3. (D) The MC3T3-E1 cells were pretreated with or <t>without</t> <t>Akti1/2</t> (5uM), and were induced to osteoblasts with PBS, E2, VitD3, E2+VitD3. The ALP staining was utilized to evaluate the osteogenic differentiation in each group.
Anti Pakt, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti pakt/product/Proteintech
Average 96 stars, based on 1 article reviews
anti pakt - by Bioz Stars, 2026-02
96/100 stars
  Buy from Supplier
akt monoclonal antibody  (Proteintech)
96
Proteintech akt monoclonal antibody
E2 and VitD3 improves osteogenesis by <t>PI3K/AKT/mTOR</t> pathway. (A) Q-PCR analysis of mRNA level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (B) The protein level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (C) The WB analysis of protein level of pan and phsopho-PI3K, pan and phospho-Akt, pan and phospho-mTOR, and pan and phospho-FOXO3 in MC3T3-E1 cells treated with PBS, E2, VitD3, E2 plus VitD3. (D) The MC3T3-E1 cells were pretreated with or <t>without</t> <t>Akti1/2</t> (5uM), and were induced to osteoblasts with PBS, E2, VitD3, E2+VitD3. The ALP staining was utilized to evaluate the osteogenic differentiation in each group.
Akt Monoclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/akt monoclonal antibody/product/Proteintech
Average 96 stars, based on 1 article reviews
akt monoclonal antibody - by Bioz Stars, 2026-02
96/100 stars
  Buy from Supplier
phospho akt ser473 monoclonal antibody  (Proteintech)
96
Proteintech phospho akt ser473 monoclonal antibody
E2 and VitD3 improves osteogenesis by <t>PI3K/AKT/mTOR</t> pathway. (A) Q-PCR analysis of mRNA level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (B) The protein level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (C) The WB analysis of protein level of pan and phsopho-PI3K, pan and phospho-Akt, pan and phospho-mTOR, and pan and phospho-FOXO3 in MC3T3-E1 cells treated with PBS, E2, VitD3, E2 plus VitD3. (D) The MC3T3-E1 cells were pretreated with or <t>without</t> <t>Akti1/2</t> (5uM), and were induced to osteoblasts with PBS, E2, VitD3, E2+VitD3. The ALP staining was utilized to evaluate the osteogenic differentiation in each group.
Phospho Akt Ser473 Monoclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phospho akt ser473 monoclonal antibody/product/Proteintech
Average 96 stars, based on 1 article reviews
phospho akt ser473 monoclonal antibody - by Bioz Stars, 2026-02
96/100 stars
  Buy from Supplier
p akt  (Proteintech)
96
Proteintech p akt
E2 and VitD3 improves osteogenesis by <t>PI3K/AKT/mTOR</t> pathway. (A) Q-PCR analysis of mRNA level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (B) The protein level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (C) The WB analysis of protein level of pan and phsopho-PI3K, pan and phospho-Akt, pan and phospho-mTOR, and pan and phospho-FOXO3 in MC3T3-E1 cells treated with PBS, E2, VitD3, E2 plus VitD3. (D) The MC3T3-E1 cells were pretreated with or <t>without</t> <t>Akti1/2</t> (5uM), and were induced to osteoblasts with PBS, E2, VitD3, E2+VitD3. The ALP staining was utilized to evaluate the osteogenic differentiation in each group.
P Akt, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p akt/product/Proteintech
Average 96 stars, based on 1 article reviews
p akt - by Bioz Stars, 2026-02
96/100 stars
  Buy from Supplier
10176 2 ap  (Proteintech)
96
Proteintech 10176 2 ap
E2 and VitD3 improves osteogenesis by <t>PI3K/AKT/mTOR</t> pathway. (A) Q-PCR analysis of mRNA level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (B) The protein level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (C) The WB analysis of protein level of pan and phsopho-PI3K, pan and phospho-Akt, pan and phospho-mTOR, and pan and phospho-FOXO3 in MC3T3-E1 cells treated with PBS, E2, VitD3, E2 plus VitD3. (D) The MC3T3-E1 cells were pretreated with or <t>without</t> <t>Akti1/2</t> (5uM), and were induced to osteoblasts with PBS, E2, VitD3, E2+VitD3. The ALP staining was utilized to evaluate the osteogenic differentiation in each group.
10176 2 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/10176 2 ap/product/Proteintech
Average 96 stars, based on 1 article reviews
10176 2 ap - by Bioz Stars, 2026-02
96/100 stars
  Buy from Supplier
akt proteintech cat 60203 2 ig  (Proteintech)
96
Proteintech akt proteintech cat 60203 2 ig
E2 and VitD3 improves osteogenesis by <t>PI3K/AKT/mTOR</t> pathway. (A) Q-PCR analysis of mRNA level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (B) The protein level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (C) The WB analysis of protein level of pan and phsopho-PI3K, pan and phospho-Akt, pan and phospho-mTOR, and pan and phospho-FOXO3 in MC3T3-E1 cells treated with PBS, E2, VitD3, E2 plus VitD3. (D) The MC3T3-E1 cells were pretreated with or <t>without</t> <t>Akti1/2</t> (5uM), and were induced to osteoblasts with PBS, E2, VitD3, E2+VitD3. The ALP staining was utilized to evaluate the osteogenic differentiation in each group.
Akt Proteintech Cat 60203 2 Ig, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/akt proteintech cat 60203 2 ig/product/Proteintech
Average 96 stars, based on 1 article reviews
akt proteintech cat 60203 2 ig - by Bioz Stars, 2026-02
96/100 stars
  Buy from Supplier
mouse  (Proteintech)
96
Proteintech mouse
E2 and VitD3 improves osteogenesis by <t>PI3K/AKT/mTOR</t> pathway. (A) Q-PCR analysis of mRNA level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (B) The protein level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (C) The WB analysis of protein level of pan and phsopho-PI3K, pan and phospho-Akt, pan and phospho-mTOR, and pan and phospho-FOXO3 in MC3T3-E1 cells treated with PBS, E2, VitD3, E2 plus VitD3. (D) The MC3T3-E1 cells were pretreated with or <t>without</t> <t>Akti1/2</t> (5uM), and were induced to osteoblasts with PBS, E2, VitD3, E2+VitD3. The ALP staining was utilized to evaluate the osteogenic differentiation in each group.
Mouse, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse/product/Proteintech
Average 96 stars, based on 1 article reviews
mouse - by Bioz Stars, 2026-02
96/100 stars
  Buy from Supplier
rabbit  (Proteintech)
96
Proteintech rabbit
E2 and VitD3 improves osteogenesis by <t>PI3K/AKT/mTOR</t> pathway. (A) Q-PCR analysis of mRNA level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (B) The protein level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (C) The WB analysis of protein level of pan and phsopho-PI3K, pan and phospho-Akt, pan and phospho-mTOR, and pan and phospho-FOXO3 in MC3T3-E1 cells treated with PBS, E2, VitD3, E2 plus VitD3. (D) The MC3T3-E1 cells were pretreated with or <t>without</t> <t>Akti1/2</t> (5uM), and were induced to osteoblasts with PBS, E2, VitD3, E2+VitD3. The ALP staining was utilized to evaluate the osteogenic differentiation in each group.
Rabbit, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit/product/Proteintech
Average 96 stars, based on 1 article reviews
rabbit - by Bioz Stars, 2026-02
96/100 stars
  Buy from Supplier

Image Search Results


E2 and VitD3 improves osteogenesis by PI3K/AKT/mTOR pathway. (A) Q-PCR analysis of mRNA level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (B) The protein level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (C) The WB analysis of protein level of pan and phsopho-PI3K, pan and phospho-Akt, pan and phospho-mTOR, and pan and phospho-FOXO3 in MC3T3-E1 cells treated with PBS, E2, VitD3, E2 plus VitD3. (D) The MC3T3-E1 cells were pretreated with or without Akti1/2 (5uM), and were induced to osteoblasts with PBS, E2, VitD3, E2+VitD3. The ALP staining was utilized to evaluate the osteogenic differentiation in each group.

Journal: Materials Today Bio

Article Title: Alendronic acid modified PLGA drug delivery system loaded with 17β-Estradiol and vitamin D3 has anti-osteoporotic effect

doi: 10.1016/j.mtbio.2026.102789

Figure Lengend Snippet: E2 and VitD3 improves osteogenesis by PI3K/AKT/mTOR pathway. (A) Q-PCR analysis of mRNA level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (B) The protein level of ERα, ERβ and VDR in Ctrl, E2, VD and E2+VD in MC3T3-E1 cells. (C) The WB analysis of protein level of pan and phsopho-PI3K, pan and phospho-Akt, pan and phospho-mTOR, and pan and phospho-FOXO3 in MC3T3-E1 cells treated with PBS, E2, VitD3, E2 plus VitD3. (D) The MC3T3-E1 cells were pretreated with or without Akti1/2 (5uM), and were induced to osteoblasts with PBS, E2, VitD3, E2+VitD3. The ALP staining was utilized to evaluate the osteogenic differentiation in each group.

Article Snippet: The AKT inhibitor (Akti1/2, MedChemExpress, USA) was dissolved in DMSO and used at a final concentration of 10 μM.

Techniques: Staining