Journal: The Journal of endocrinology

Article Title: Growth factor-induced signaling of the pancreatic epithelium.

doi: 10.1677/joe.1.05949

Figure Lengend Snippet: Figure 5 Phospho-Akt and Akt staining in the IFN transgenic pancreas. Pancreatic sections from NOD (A and C) and IFNNOD (B and D). (A and B) Sections stained with an antibody to phospho-Akt (Ser473). A significant number of cells in the proliferating ducts and cells contiguous to the duct cells exhibit phospho-Akt expression in the transgenic mouse pancreas. Interestingly, phospho-Akt is also expressed in the periphery of the islets in the NOD pancreas in a pattern similar to glucagon staining. (C and D) Sections stained with an antibody to Akt. Original magnification 50.

Article Snippet: The polyclonal phospho-Akt antibodies were generated against a synthetic phospho-Ser473 peptide corresponding to residues around mouse Akt (Cell Signaling Technology).

Techniques: Staining, Transgenic Assay, Expressing

Journal: Nutrients

Article Title: Changes Induced by Aging and Long-Term Exercise and/or DHA Supplementation in Muscle of Obese Female Mice.

doi: 10.3390/nu14204240

Figure Lengend Snippet: Figure 2. Representative Western blot and densitometry analysis of the effects of aging (2, 6 and 18 months) on phosphorylated AKT and total AKT (A) and phosphorylated ACC and total ACC (B) in the gastrocnemius muscle of C57BL/6J female mice fed a standard diet. Band densities of phosphorylated AKT and ACC were normalized by total AKT and ACC, respectively. Data presented as mean (SD); n = 3–4.

Article Snippet: Next, membranes were blocked (5% bovine serum album) for 1 h at room temperature and incubated overnight at 4 ◦C with specific primary antibodies at 1:1000 against: Phospho-AKT (p-AKT) (rabbit, Ser473), AKT (rabbit), Phospho-Acetyl-CoA Carboxylase (p-ACC) (rabbit, Ser79), Acetyl-CoA Carboxylase (ACC) (rabbit) (Cell Signaling Technology, Danvers, MA, USA) and β-Actin (mouse, Sigma-Aldrich).

Techniques: Western Blot

Journal: Nutrients

Article Title: Changes Induced by Aging and Long-Term Exercise and/or DHA Supplementation in Muscle of Obese Female Mice.

doi: 10.3390/nu14204240

Figure Lengend Snippet: Figure 4. Representative Western blot and densitometry analysis of the effects of long-term DHA supplementation and/or exercise training on phosphorylated AKT and total AKT (A) and phospho- rylated ACC and total ACC (B) in the gastrocnemius muscle of 18-month-old DIO female mice. Band densities of phosphorylated AKT and phosphorylated ACC were normalized by total AKT and total ACC, respectively. Data presented as mean (SD); n = 3–4. The p values for the main factors, DHA supplementation (DHA), exercise (EX) and the interaction between both (DHAxEX) are shown in the corresponding rows of the tables below the figures. Statistically significant p values are marked in bold.

Article Snippet: Next, membranes were blocked (5% bovine serum album) for 1 h at room temperature and incubated overnight at 4 ◦C with specific primary antibodies at 1:1000 against: Phospho-AKT (p-AKT) (rabbit, Ser473), AKT (rabbit), Phospho-Acetyl-CoA Carboxylase (p-ACC) (rabbit, Ser79), Acetyl-CoA Carboxylase (ACC) (rabbit) (Cell Signaling Technology, Danvers, MA, USA) and β-Actin (mouse, Sigma-Aldrich).

Techniques: Western Blot