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jak2 inhibitor ag490 (MedChemExpress)

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MedChemExpress jak2 inhibitor ag490
Jak2 Inhibitor Ag490, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 222 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ag490/AG490/10__1007_slash_s42995___026___00415___z-101-36-39
Average 97 stars, based on 222 article reviews

jak2 inhibitor ag490 - by Bioz Stars, 2026-10

97/100 stars

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Article Title: (Ba,Ca)(Ti,Sn)O 3 -based piezoelectric ceramics promotes neuroprotection by regulating microglial IL-6/JAK2/STAT3 signaling pathway
Article Snippet: JSM-5900 Scanning Electron Microscope (Japan), Xpert-PRO X-ray Diffractometer (Netherlands), Water Contact Angle Goniometer JC2000D1 (Shanghai Zhongchen Digital Technology Equipment Co., Ltd., China), Quasi-static d 33 Meter ZJ-3AN (Institute of Acoustics, Chinese Academy of Sciences, China), Ultrasound Stimulator (GV-CSI1.0) (Green Valley BrainTech (ShenZhen) Medical Technology Co., Ltd. (China), BV2, PC12 (Wuhan Pricella Biotechnology Co., Ltd., China), Rabbit Anti-iNOS (Thermo Fisher Scientific, Inc.), Rabbit Anti-Iba-1, Arg-1, NeuN, Goat Anti-Rabbit IgG H&L (Alexa Fluor 488), Goat Anti-Mouse IgG H&L (Alexa Fluor 594) (Abcam, UK), Rabbit Anti-CD86, CD206, β-actin, HRP-conjugated Goat Anti-Rabbit IgG (H + L) (Wuhan Proteintech Biotechnology Co., Ltd., China), Rabbit Anti-IL-6, TNF-α, IL-4, IL-10, TGF-β1 (ImmunoWay, United States), Rabbit Anti-IL-1β, JAK2, P-JAK2, STAT3, P-STAT3, BAX, Bcl-2, Cleaved Caspase-3 (Shenyang Wanlei Biotechnology Co., Ltd., China), BCA Protein Assay Kit, CCK8 Cell Viability Assay Kit, LDH Cytotoxicity Assay Kit, SOD Activity Assay Kit, CAT Activity Assay Kit, GSH-Px Activity Assay Kit, MDA Assay Kit, Dapi (Beijing Solarbio Science & Technology Co., Ltd., China), LPS (Sigma, Germany), IL-6, AG490 (MCE, Germany), Laser Confocal Microscope (Olympus Corporation, Japan), SDS-PAGE Electrophoresis System (BIO-RAD, USA).

Cell Culture:

Article Title: Macrophage-derived legumain ameliorates excessive mechanical stress-induced ferroptosis of nucleus pulposus cells and intervertebral disc degeneration via integrin αvβ3-Hippo signaling.
Article Snippet: .. In a separate experiment, BMDMs plated on insert membranes were supplemented with 20 μM AG490 (MCE, HY‐12000) while NPCs were cultured on the well‐plate surfaces. ..

Activation Assay:

Article Title: Oncostatin M promotes immune escape, invasion, and metastasis of pancreatic cancer by upregulating PD-L1 via the PI3K/AKT pathway.
Article Snippet: Pancreatic cancer is characterized by rapid progression and high mortality, while the overall benefit of immune checkpoint inhibitors remains limited, highlighting the need for more effective immunotherapeutic strategies.. Through bioinformatic screening and clinical tissue analyses, we found that oncostatin M (OSM) expression is closely associated with both PD-L1 levels and tumor metastasis in pancreatic cancer, suggesting its potential as a novel immunotherapeutic target.. Western blot (WB), qPCR, and flow cytometry demonstrated that OSM markedly upregulates PD-L1 expression at both the transcriptional and protein levels in pancreatic cancer cells.

Blocking Assay:

Control:

Article Title: Keratinocytes regulate intraepithelial lymphocytes homing and mediate mucosal barrier integrity via JAK2/STAT3 signaling in oral lichen planus
Article Snippet: .. The models were divided into four groups based on the addition of inhibitors: (1) Control group (treated with K-SFM medium containing an equal concentration of PBS); (2) AG490 group (treated with K-SFM medium containing 50 μmol/L AG490) (AG490, Cat # HY-12003, MCE); (3) RPM group (treated with K-SFM medium containing 20 nmol/L RPM) (RPM, Cat # HY-10219, MCE); and (4) AG490+RPM group (treated with K-SFM medium containing 50 μmol/L AG490 and 20 nmol/L RPM). ..

Concentration Assay:

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jak2 inhibitor ag490 (MedChemExpress)

MedChemExpress jak2 inhibitor ag490
Jak2 Inhibitor Ag490, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ag490/AG490/10__1007_slash_s42995___026___00415___z-101-36-39
Average 97 stars, based on 1 article reviews

jak2 inhibitor ag490 - by Bioz Stars, 2026-10

97/100 stars

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ag490 (Santa Cruz Biotechnology)

Santa Cruz Biotechnology ag490
$Inhibition of STAT3 enhances MeHg-induced Nrf2 activity in mouse hypothalamic neuronal GT1-7 cells. A Representative western blot analysis of Nrf2 protein levels in cytoplasmic and nuclear fractions, with densitometry quantification of nuclear Nrf2 protein levels in GT1-7 cells pre-treated with 100 µM <t>AG490</t> or 30 µM C188-9 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 9 independent cell culture preparations). B qPCR analysis of Nfe2l2 mRNA levels in GT1-7 cells treated with 10 or 100 µM AG490 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 7 independent cell culture preparations). C qPCR analysis of Keap1 mRNA levels in GT1-7 cells treated with 10 or 100 µM AG490 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 7 independent cell culture preparations). D qPCR analysis of Slc7a11 mRNA levels in GT1-7 cells treated with 10 or 100 µM AG490 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 7 independent cell culture preparations). E qPCR analysis of Nqo1 mRNA levels in GT1-7 cells treated with 10 or 100 µM AG490 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 7 independent cell culture preparations). F qPCR analysis of Nfe2l2 mRNA levels in GT1-7 cells treated with 15 or 30 µM C188-9 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 7 independent cell culture preparations).) qPCR analysis of Keap1 mRNA levels in GT1-7 cells treated with 15 or 30 µM C188-9 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 7 independent cell culture preparations). H qPCR analysis of Slc7a11 mRNA levels in GT1-7 cells treated with 15 or 30 µM C188-9 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 6–7 independent cell culture preparations). I qPCR analysis of Nqo1 mRNA levels in GT1-7 cells treated with 15 or 30 µM C188-9 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 7 independent cell culture preparations). Data are expressed as mean ± SD. * indicates p < 0.05$
Ag490, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ag490/AG-490/pmc13649924-72-21-24
Average 93 stars, based on 1 article reviews

ag490 - by Bioz Stars, 2026-10

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ag490 (MedChemExpress)

MedChemExpress ag490
$Inhibition of STAT3 enhances MeHg-induced Nrf2 activity in mouse hypothalamic neuronal GT1-7 cells. A Representative western blot analysis of Nrf2 protein levels in cytoplasmic and nuclear fractions, with densitometry quantification of nuclear Nrf2 protein levels in GT1-7 cells pre-treated with 100 µM <t>AG490</t> or 30 µM C188-9 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 9 independent cell culture preparations). B qPCR analysis of Nfe2l2 mRNA levels in GT1-7 cells treated with 10 or 100 µM AG490 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 7 independent cell culture preparations). C qPCR analysis of Keap1 mRNA levels in GT1-7 cells treated with 10 or 100 µM AG490 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 7 independent cell culture preparations). D qPCR analysis of Slc7a11 mRNA levels in GT1-7 cells treated with 10 or 100 µM AG490 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 7 independent cell culture preparations). E qPCR analysis of Nqo1 mRNA levels in GT1-7 cells treated with 10 or 100 µM AG490 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 7 independent cell culture preparations). F qPCR analysis of Nfe2l2 mRNA levels in GT1-7 cells treated with 15 or 30 µM C188-9 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 7 independent cell culture preparations).) qPCR analysis of Keap1 mRNA levels in GT1-7 cells treated with 15 or 30 µM C188-9 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 7 independent cell culture preparations). H qPCR analysis of Slc7a11 mRNA levels in GT1-7 cells treated with 15 or 30 µM C188-9 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 6–7 independent cell culture preparations). I qPCR analysis of Nqo1 mRNA levels in GT1-7 cells treated with 15 or 30 µM C188-9 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 7 independent cell culture preparations). Data are expressed as mean ± SD. * indicates p < 0.05$
Ag490, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ag490/AG490/pm42462475-65-0-17
Average 97 stars, based on 1 article reviews

ag490 - by Bioz Stars, 2026-10

97/100 stars

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chemical inhibitors ag490 (MedChemExpress)

MedChemExpress chemical inhibitors ag490
$Inhibition of STAT3 enhances MeHg-induced Nrf2 activity in mouse hypothalamic neuronal GT1-7 cells. A Representative western blot analysis of Nrf2 protein levels in cytoplasmic and nuclear fractions, with densitometry quantification of nuclear Nrf2 protein levels in GT1-7 cells pre-treated with 100 µM <t>AG490</t> or 30 µM C188-9 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 9 independent cell culture preparations). B qPCR analysis of Nfe2l2 mRNA levels in GT1-7 cells treated with 10 or 100 µM AG490 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 7 independent cell culture preparations). C qPCR analysis of Keap1 mRNA levels in GT1-7 cells treated with 10 or 100 µM AG490 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 7 independent cell culture preparations). D qPCR analysis of Slc7a11 mRNA levels in GT1-7 cells treated with 10 or 100 µM AG490 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 7 independent cell culture preparations). E qPCR analysis of Nqo1 mRNA levels in GT1-7 cells treated with 10 or 100 µM AG490 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 7 independent cell culture preparations). F qPCR analysis of Nfe2l2 mRNA levels in GT1-7 cells treated with 15 or 30 µM C188-9 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 7 independent cell culture preparations).) qPCR analysis of Keap1 mRNA levels in GT1-7 cells treated with 15 or 30 µM C188-9 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 7 independent cell culture preparations). H qPCR analysis of Slc7a11 mRNA levels in GT1-7 cells treated with 15 or 30 µM C188-9 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 6–7 independent cell culture preparations). I qPCR analysis of Nqo1 mRNA levels in GT1-7 cells treated with 15 or 30 µM C188-9 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 7 independent cell culture preparations). Data are expressed as mean ± SD. * indicates p < 0.05$
Chemical Inhibitors Ag490, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ag490/AG490/pm42032729-43-0-9
Average 97 stars, based on 1 article reviews

chemical inhibitors ag490 - by Bioz Stars, 2026-10

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ag490 cat hy 12003 mce (MedChemExpress)

MedChemExpress ag490 cat hy 12003 mce

p-JAK2/JAK2 and p-STAT3/STAT3 were highly expressed in OLP. <t>AG490</t> and RPM inhibited KCs proliferation. AG490 and RPM inhibited homing migration of OLP IELs. (A–C) p-JAK2/JAK2 and p-STAT3/STAT3 were significantly upregulated in OLP compared to controls (n = 6), as detected by western blot. (D) In the MTT assay, at 48 h, the OD450 values of the AG490 group, RPM group, and AG490+RPM group were significantly lower than those of the Control group. Compared with the AG490 group and RPM group, the OD450 values of the AG490+RPM group were significantly reduced. Additionally, the OD450 values of the AG490 group were significantly lower than those of the RPM group. (E) Compared with the control group, the AG490 group and RPM group exhibited a significant reduction in the number of migration tracks, while the AG490+RPM group showed a significantly reduced number of tracks. (F, G) Compared with the control group, the AG490 group and RPM group demonstrated a significant decrease in both speed and maximum displacement, and the AG490+RPM group exhibited a significant reduction in both speed and maximum displacement. * p < 0.05; ** p < 0.01; *** p < 0.001. JAK2, Janus kinase 2; p-JAK2, Phosphorylated Janus kinase 2; STAT3, Signal transducer and activator of transcription 3; p-STAT3, Phosphorylated signal transducer and activator of transcription 3; OD, Optical density; AG490, (E)-N-Benzyl-2-cyano-3-(3,4-dihydroxyphenyl)prop-2-enamide; RPM, Ruxolitinib.

Ag490 Cat Hy 12003 Mce, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ag490/SNS-314/pmc13070951-138-37-41
Average 94 stars, based on 1 article reviews

ag490 cat hy 12003 mce - by Bioz Stars, 2026-10

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$Inhibition of STAT3 enhances MeHg-induced Nrf2 activity in mouse hypothalamic neuronal GT1-7 cells. A Representative western blot analysis of Nrf2 protein levels in cytoplasmic and nuclear fractions, with densitometry quantification of nuclear Nrf2 protein levels in GT1-7 cells pre-treated with 100 µM AG490 or 30 µM C188-9 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 9 independent cell culture preparations). B qPCR analysis of Nfe2l2 mRNA levels in GT1-7 cells treated with 10 or 100 µM AG490 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 7 independent cell culture preparations). C qPCR analysis of Keap1 mRNA levels in GT1-7 cells treated with 10 or 100 µM AG490 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 7 independent cell culture preparations). D qPCR analysis of Slc7a11 mRNA levels in GT1-7 cells treated with 10 or 100 µM AG490 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 7 independent cell culture preparations). E qPCR analysis of Nqo1 mRNA levels in GT1-7 cells treated with 10 or 100 µM AG490 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 7 independent cell culture preparations). F qPCR analysis of Nfe2l2 mRNA levels in GT1-7 cells treated with 15 or 30 µM C188-9 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 7 independent cell culture preparations).) qPCR analysis of Keap1 mRNA levels in GT1-7 cells treated with 15 or 30 µM C188-9 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 7 independent cell culture preparations). H qPCR analysis of Slc7a11 mRNA levels in GT1-7 cells treated with 15 or 30 µM C188-9 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 6–7 independent cell culture preparations). I qPCR analysis of Nqo1 mRNA levels in GT1-7 cells treated with 15 or 30 µM C188-9 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 7 independent cell culture preparations). Data are expressed as mean ± SD. * indicates p < 0.05$

Journal: Neurochemical Research

Article Title: Nrf2 and STAT3 Pathways in Protecting Against Methylmercury-Induced Oxidative Stress in Hypothalamic Neuronal GT1-7 Cells

doi: 10.1007/s11064-026-04898-1

Figure Lengend Snippet: Inhibition of STAT3 enhances MeHg-induced Nrf2 activity in mouse hypothalamic neuronal GT1-7 cells. A Representative western blot analysis of Nrf2 protein levels in cytoplasmic and nuclear fractions, with densitometry quantification of nuclear Nrf2 protein levels in GT1-7 cells pre-treated with 100 µM AG490 or 30 µM C188-9 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 9 independent cell culture preparations). B qPCR analysis of Nfe2l2 mRNA levels in GT1-7 cells treated with 10 or 100 µM AG490 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 7 independent cell culture preparations). C qPCR analysis of Keap1 mRNA levels in GT1-7 cells treated with 10 or 100 µM AG490 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 7 independent cell culture preparations). D qPCR analysis of Slc7a11 mRNA levels in GT1-7 cells treated with 10 or 100 µM AG490 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 7 independent cell culture preparations). E qPCR analysis of Nqo1 mRNA levels in GT1-7 cells treated with 10 or 100 µM AG490 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 7 independent cell culture preparations). F qPCR analysis of Nfe2l2 mRNA levels in GT1-7 cells treated with 15 or 30 µM C188-9 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 7 independent cell culture preparations).) qPCR analysis of Keap1 mRNA levels in GT1-7 cells treated with 15 or 30 µM C188-9 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 7 independent cell culture preparations). H qPCR analysis of Slc7a11 mRNA levels in GT1-7 cells treated with 15 or 30 µM C188-9 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 6–7 independent cell culture preparations). I qPCR analysis of Nqo1 mRNA levels in GT1-7 cells treated with 15 or 30 µM C188-9 for 1 h, followed by co-exposure to 5 µM MeHg for 6 h ( n = 7 independent cell culture preparations). Data are expressed as mean ± SD. * indicates p < 0.05

Article Snippet: To investigate the role of STAT3 in Nrf2 induction, cells were pre-treated for 1 h with either 10 or 100 μM AG490 (Tyrphostin B42, Santa Cruz Biotechnology, Santa Cruz biotechnology, Inc. cat. no. sc-202046) or with 15 or 30 μM of STAT3 Inhibitor XIII, C188-9 (CalbioChem, cat. no. 573128).

Techniques: Inhibition, Activity Assay, Western Blot, Analysis, Cell Culture

p-JAK2/JAK2 and p-STAT3/STAT3 were highly expressed in OLP. AG490 and RPM inhibited KCs proliferation. AG490 and RPM inhibited homing migration of OLP IELs. (A–C) p-JAK2/JAK2 and p-STAT3/STAT3 were significantly upregulated in OLP compared to controls (n = 6), as detected by western blot. (D) In the MTT assay, at 48 h, the OD450 values of the AG490 group, RPM group, and AG490+RPM group were significantly lower than those of the Control group. Compared with the AG490 group and RPM group, the OD450 values of the AG490+RPM group were significantly reduced. Additionally, the OD450 values of the AG490 group were significantly lower than those of the RPM group. (E) Compared with the control group, the AG490 group and RPM group exhibited a significant reduction in the number of migration tracks, while the AG490+RPM group showed a significantly reduced number of tracks. (F, G) Compared with the control group, the AG490 group and RPM group demonstrated a significant decrease in both speed and maximum displacement, and the AG490+RPM group exhibited a significant reduction in both speed and maximum displacement. * p < 0.05; ** p < 0.01; *** p < 0.001. JAK2, Janus kinase 2; p-JAK2, Phosphorylated Janus kinase 2; STAT3, Signal transducer and activator of transcription 3; p-STAT3, Phosphorylated signal transducer and activator of transcription 3; OD, Optical density; AG490, (E)-N-Benzyl-2-cyano-3-(3,4-dihydroxyphenyl)prop-2-enamide; RPM, Ruxolitinib.

Journal: Frontiers in Immunology

Article Title: Keratinocytes regulate intraepithelial lymphocytes homing and mediate mucosal barrier integrity via JAK2/STAT3 signaling in oral lichen planus

doi: 10.3389/fimmu.2026.1794867

Figure Lengend Snippet: p-JAK2/JAK2 and p-STAT3/STAT3 were highly expressed in OLP. AG490 and RPM inhibited KCs proliferation. AG490 and RPM inhibited homing migration of OLP IELs. (A–C) p-JAK2/JAK2 and p-STAT3/STAT3 were significantly upregulated in OLP compared to controls (n = 6), as detected by western blot. (D) In the MTT assay, at 48 h, the OD450 values of the AG490 group, RPM group, and AG490+RPM group were significantly lower than those of the Control group. Compared with the AG490 group and RPM group, the OD450 values of the AG490+RPM group were significantly reduced. Additionally, the OD450 values of the AG490 group were significantly lower than those of the RPM group. (E) Compared with the control group, the AG490 group and RPM group exhibited a significant reduction in the number of migration tracks, while the AG490+RPM group showed a significantly reduced number of tracks. (F, G) Compared with the control group, the AG490 group and RPM group demonstrated a significant decrease in both speed and maximum displacement, and the AG490+RPM group exhibited a significant reduction in both speed and maximum displacement. * p < 0.05; ** p < 0.01; *** p < 0.001. JAK2, Janus kinase 2; p-JAK2, Phosphorylated Janus kinase 2; STAT3, Signal transducer and activator of transcription 3; p-STAT3, Phosphorylated signal transducer and activator of transcription 3; OD, Optical density; AG490, (E)-N-Benzyl-2-cyano-3-(3,4-dihydroxyphenyl)prop-2-enamide; RPM, Ruxolitinib.

Article Snippet: The models were divided into four groups based on the addition of inhibitors: (1) Control group (treated with K-SFM medium containing an equal concentration of PBS); (2) AG490 group (treated with K-SFM medium containing 50 μmol/L AG490) (AG490, Cat # HY-12003, MCE); (3) RPM group (treated with K-SFM medium containing 20 nmol/L RPM) (RPM, Cat # HY-10219, MCE); and (4) AG490+RPM group (treated with K-SFM medium containing 50 μmol/L AG490 and 20 nmol/L RPM).

Techniques: Migration, Western Blot, MTT Assay, Control

AG490 and RPM inhibited the expression of apoptosis-related proteins Bax and caspase-3 and promoted the expression of Bcl-2, as measured by ELISA. (a), compared with control, p < 0.05; (b), compared with AG490 group, p < 0.05; (c), compared with RPM group, p < 0.05. AG490, (E)-N-Benzyl-2-cyano-3-(3,4-dihydroxyphenyl)prop-2-enamide; RPM, Ruxolitinib; Bax, Bcl-2 associated X protein; Bcl-2, B-cell lymphoma/leukemia-2 gene; caspase-3, Cysteine-dependent aspartate-specific protease-3.

Journal: Frontiers in Immunology

Article Title: Keratinocytes regulate intraepithelial lymphocytes homing and mediate mucosal barrier integrity via JAK2/STAT3 signaling in oral lichen planus

doi: 10.3389/fimmu.2026.1794867

Figure Lengend Snippet: AG490 and RPM inhibited the expression of apoptosis-related proteins Bax and caspase-3 and promoted the expression of Bcl-2, as measured by ELISA. (a), compared with control, p < 0.05; (b), compared with AG490 group, p < 0.05; (c), compared with RPM group, p < 0.05. AG490, (E)-N-Benzyl-2-cyano-3-(3,4-dihydroxyphenyl)prop-2-enamide; RPM, Ruxolitinib; Bax, Bcl-2 associated X protein; Bcl-2, B-cell lymphoma/leukemia-2 gene; caspase-3, Cysteine-dependent aspartate-specific protease-3.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Control

AG490 and RPM synergistically inhibited the phosphorylation of JAK2/STAT3. (A, B) AG490 and RPM selectively inhibited JAK2/STAT3 phosphorylation. * p < 0.05; ** p < 0.01; *** p < 0.001. (C) JAK2 and p-JAK2 were downregulated in AG490 and AG490+RPM group. (D) STAT3 and p-STAT3 were downregulated in RPM and AG490+RPM group. (E) Compared with the control group, the protein ratios of p-JAK2/JAK2 and p-STAT3/STAT3 were significantly increased in the AG490 group, RPM group, and AG490+RPM group. Compared with the AG490 group and RPM group, the protein ratios of p-JAK2/JAK2 and p-STAT3/STAT3 were significantly decreased in the AG490+RPM group. a, compared with control, p < 0.05; b, compared with AG490 group, p < 0.05; c, compared with RPM group, p < 0.05; aa, compared with control, p < 0.01. AG490, (E)-N-Benzyl-2-cyano-3-(3,4-dihydroxyphenyl)prop-2-enamide; RPM, Ruxolitinib; JAK2, Janus kinase 2; p-JAK2, Phosphorylated Janus kinase 2; STAT3, Signal transducer and activator of transcription 3; p-STAT3: Phosphorylated signal transducer and activator of transcription 3; GAPDH, Glycerol-3-phosphate dehydrogenase.

Journal: Frontiers in Immunology

Article Title: Keratinocytes regulate intraepithelial lymphocytes homing and mediate mucosal barrier integrity via JAK2/STAT3 signaling in oral lichen planus

doi: 10.3389/fimmu.2026.1794867

Figure Lengend Snippet: AG490 and RPM synergistically inhibited the phosphorylation of JAK2/STAT3. (A, B) AG490 and RPM selectively inhibited JAK2/STAT3 phosphorylation. * p < 0.05; ** p < 0.01; *** p < 0.001. (C) JAK2 and p-JAK2 were downregulated in AG490 and AG490+RPM group. (D) STAT3 and p-STAT3 were downregulated in RPM and AG490+RPM group. (E) Compared with the control group, the protein ratios of p-JAK2/JAK2 and p-STAT3/STAT3 were significantly increased in the AG490 group, RPM group, and AG490+RPM group. Compared with the AG490 group and RPM group, the protein ratios of p-JAK2/JAK2 and p-STAT3/STAT3 were significantly decreased in the AG490+RPM group. a, compared with control, p < 0.05; b, compared with AG490 group, p < 0.05; c, compared with RPM group, p < 0.05; aa, compared with control, p < 0.01. AG490, (E)-N-Benzyl-2-cyano-3-(3,4-dihydroxyphenyl)prop-2-enamide; RPM, Ruxolitinib; JAK2, Janus kinase 2; p-JAK2, Phosphorylated Janus kinase 2; STAT3, Signal transducer and activator of transcription 3; p-STAT3: Phosphorylated signal transducer and activator of transcription 3; GAPDH, Glycerol-3-phosphate dehydrogenase.

Techniques: Phospho-proteomics, Control

The expression of ZO-1 and Occludin mRNA was upregulated after using AG490 and RPM. (A, B) Compared with the control group, the expression of ZO-1 and Occludin mRNA increased in the AG490 group. The expression of ZO-1 and Occludin mRAN in the RPM group was upregulated. The expression of ZO-1 and Occludin mRNA was significantly increased in the AG490+RPM group. Compared with the RPM group, the AG490+RPM group showed increased expression of ZO-1 and Occludin mRNA. * p < 0.05; ** p < 0.01; *** p < 0.001. (C) Schematic diagram of the regulatory network mechanism involving E-cadherin/CD103-JAK2/STAT3-ZO-1/Occludin in OLP. The binding of E-cadherin/CD103 downregulated JAK2/STAT3 phosphorylation in KCs and upregulated mucosal barrier molecules ZO-1 and Occludin, which helped maintain the integrity of the mucosal barrier. CD103, Integrin alpha-E; E-cadherin, Epithelial cadherin; JAK2, Janus kinase 2; STAT3, Signal transducer and activator of transcription 3; AG490, (E)-N-Benzyl-2-cyano-3-(3,4-dihydroxyphenyl)prop-2-enamide; RPM, Ruxolitinib; ZO-1, Zonula occludens protein 1; KC, Keratinocytes; IEL, Intraepithelial lymphocyte.

Journal: Frontiers in Immunology

Article Title: Keratinocytes regulate intraepithelial lymphocytes homing and mediate mucosal barrier integrity via JAK2/STAT3 signaling in oral lichen planus

doi: 10.3389/fimmu.2026.1794867

Figure Lengend Snippet: The expression of ZO-1 and Occludin mRNA was upregulated after using AG490 and RPM. (A, B) Compared with the control group, the expression of ZO-1 and Occludin mRNA increased in the AG490 group. The expression of ZO-1 and Occludin mRAN in the RPM group was upregulated. The expression of ZO-1 and Occludin mRNA was significantly increased in the AG490+RPM group. Compared with the RPM group, the AG490+RPM group showed increased expression of ZO-1 and Occludin mRNA. * p < 0.05; ** p < 0.01; *** p < 0.001. (C) Schematic diagram of the regulatory network mechanism involving E-cadherin/CD103-JAK2/STAT3-ZO-1/Occludin in OLP. The binding of E-cadherin/CD103 downregulated JAK2/STAT3 phosphorylation in KCs and upregulated mucosal barrier molecules ZO-1 and Occludin, which helped maintain the integrity of the mucosal barrier. CD103, Integrin alpha-E; E-cadherin, Epithelial cadherin; JAK2, Janus kinase 2; STAT3, Signal transducer and activator of transcription 3; AG490, (E)-N-Benzyl-2-cyano-3-(3,4-dihydroxyphenyl)prop-2-enamide; RPM, Ruxolitinib; ZO-1, Zonula occludens protein 1; KC, Keratinocytes; IEL, Intraepithelial lymphocyte.

Techniques: Expressing, Control, Binding Assay, Phospho-proteomics

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Activation Assay:

Blocking Assay:

Control:

Concentration Assay:

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