afm1  (Millipore)


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    Name:
    Aflatoxin M1
    Description:

    Catalog Number:
    a6428
    Price:
    None
    Applications:
    Aflatoxin M1 is both a hepatotoxic and hepatocarcinogenic mycotoxin that has been shown to cause immunosuppression in animals.
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    Structured Review

    Millipore afm1
    Aflatoxin M1

    https://www.bioz.com/result/afm1/product/Millipore
    Average 95 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    afm1 - by Bioz Stars, 2020-09
    95/100 stars

    Images

    1) Product Images from "Turn-On Fluorescence Aptasensor on Magnetic Nanobeads for Aflatoxin M1 Detection Based on an Exonuclease III-Assisted Signal Amplification Strategy"

    Article Title: Turn-On Fluorescence Aptasensor on Magnetic Nanobeads for Aflatoxin M1 Detection Based on an Exonuclease III-Assisted Signal Amplification Strategy

    Journal: Nanomaterials

    doi: 10.3390/nano9010104

    Additive study by adding different concentrations of AFM1 (20, 50 and 100 µg kg −1 ) in ( a ) whole milk, ( b ) skimmed milk and ( c ) juice milk. The obtained results by the developed aptasensor and commercial ELISA kits are compared (in white) ( n = 3).
    Figure Legend Snippet: Additive study by adding different concentrations of AFM1 (20, 50 and 100 µg kg −1 ) in ( a ) whole milk, ( b ) skimmed milk and ( c ) juice milk. The obtained results by the developed aptasensor and commercial ELISA kits are compared (in white) ( n = 3).

    Techniques Used: Enzyme-linked Immunosorbent Assay

    Selectivity evaluation of the developed aptasensor for the detection of AFM1 (1 ng mL −1 ) against other control mycotoxins of AFB1, AFM2 and Ochratoxin A at the same concentration.
    Figure Legend Snippet: Selectivity evaluation of the developed aptasensor for the detection of AFM1 (1 ng mL −1 ) against other control mycotoxins of AFB1, AFM2 and Ochratoxin A at the same concentration.

    Techniques Used: Concentration Assay

    Schematic representation of sensitive fluorescent detection of AFM1 based on Exo III-assistant signal amplification recycles.
    Figure Legend Snippet: Schematic representation of sensitive fluorescent detection of AFM1 based on Exo III-assistant signal amplification recycles.

    Techniques Used: Amplification

    The optimization of the experiment: ( a ) Different amount of first-step resultant solution was submitted to the Exo III-assisted recycling amplification in the presence of AFM1. ( n = 3); ( b ) The AFM1 activated first-step displacement time; ( c ) The fluorescence spectra changes according to the concentration of AFM1 (from top to bottom: 20 ng mL −1 to 0 ng mL −1 ); ( d ) The calibration curve was achieved under optimized conditions.
    Figure Legend Snippet: The optimization of the experiment: ( a ) Different amount of first-step resultant solution was submitted to the Exo III-assisted recycling amplification in the presence of AFM1. ( n = 3); ( b ) The AFM1 activated first-step displacement time; ( c ) The fluorescence spectra changes according to the concentration of AFM1 (from top to bottom: 20 ng mL −1 to 0 ng mL −1 ); ( d ) The calibration curve was achieved under optimized conditions.

    Techniques Used: Amplification, Fluorescence, Concentration Assay

    2) Product Images from "Evaluation of Aflatoxin M1 Effects on the Metabolomic and Cytokinomic Profiling of a Hepatoblastoma Cell Line"

    Article Title: Evaluation of Aflatoxin M1 Effects on the Metabolomic and Cytokinomic Profiling of a Hepatoblastoma Cell Line

    Journal: Toxins

    doi: 10.3390/toxins10110436

    Partial least squares-discriminant analysis (PLS-DA) ( A ) and variable importance in projection (VIP) ( B ) plots related to the lipidic fraction of the HepG2 cell line treated with AFM1 compared to untreated cells.
    Figure Legend Snippet: Partial least squares-discriminant analysis (PLS-DA) ( A ) and variable importance in projection (VIP) ( B ) plots related to the lipidic fraction of the HepG2 cell line treated with AFM1 compared to untreated cells.

    Techniques Used:

    ( A ) Cell viability related to HepG2 cells after AFM1 treatment for 48 h. ( B ) Percentage of live, apoptotic, and dead cells (mean ± standard deviation) for HepG2 cells at IC 50 concentration before (CTRL) and after (AFM1) 48 h of treatment. ( C ) Cell percentages in G0/G1, S, and G2/M phases (mean ± standard deviation) for HepG2 cells at IC 50 concentration before (CTRL) and after (AFM1) 48 h of treatment.
    Figure Legend Snippet: ( A ) Cell viability related to HepG2 cells after AFM1 treatment for 48 h. ( B ) Percentage of live, apoptotic, and dead cells (mean ± standard deviation) for HepG2 cells at IC 50 concentration before (CTRL) and after (AFM1) 48 h of treatment. ( C ) Cell percentages in G0/G1, S, and G2/M phases (mean ± standard deviation) for HepG2 cells at IC 50 concentration before (CTRL) and after (AFM1) 48 h of treatment.

    Techniques Used: Standard Deviation, Concentration Assay

    Partial least squares-discriminant analysis (PLS-DA) ( A ) and variable importance in projection (VIP) ( B ) plots related to the polar fraction of the HepG2 cell line treated with AFM1 compared to untreated cells.
    Figure Legend Snippet: Partial least squares-discriminant analysis (PLS-DA) ( A ) and variable importance in projection (VIP) ( B ) plots related to the polar fraction of the HepG2 cell line treated with AFM1 compared to untreated cells.

    Techniques Used:

    3) Product Images from "Curcumin Protects β-Lactoglobulin Fibril Formation and Fibril-Induced Neurotoxicity in PC12Cells"

    Article Title: Curcumin Protects β-Lactoglobulin Fibril Formation and Fibril-Induced Neurotoxicity in PC12Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0133206

    ThT fluorescence of β-Lg (5 mg/mL) at pH 2 heated at 80°C. (A) In the absence and presence of Pb 2+ , curcumin and their mixture. (B) In the absence and presence of AFM1, curcumin and their mixture. Data represent the means ± SD of 3 independent measurements.
    Figure Legend Snippet: ThT fluorescence of β-Lg (5 mg/mL) at pH 2 heated at 80°C. (A) In the absence and presence of Pb 2+ , curcumin and their mixture. (B) In the absence and presence of AFM1, curcumin and their mixture. Data represent the means ± SD of 3 independent measurements.

    Techniques Used: Fluorescence

    The protective effect of curcuminon β-Lg fibrils-induced cytotoxicity of PC12 cells. PC12 cells were incubated with or without β-Lg fibrils, obtained in the absence and/or presence of curcumin, Pb 2+ , or AFM1. The PC12 cell viability was determined using MTT assay after incubation for 4 or 8 h. Incubation with β-Lg fibrils containing curcumin significantly decreased the amount of cell death compared with β-Lg fibrils in the absence of curcumin. Data represent the means ± S.E.M (n = 3; *significantly different from control).
    Figure Legend Snippet: The protective effect of curcuminon β-Lg fibrils-induced cytotoxicity of PC12 cells. PC12 cells were incubated with or without β-Lg fibrils, obtained in the absence and/or presence of curcumin, Pb 2+ , or AFM1. The PC12 cell viability was determined using MTT assay after incubation for 4 or 8 h. Incubation with β-Lg fibrils containing curcumin significantly decreased the amount of cell death compared with β-Lg fibrils in the absence of curcumin. Data represent the means ± S.E.M (n = 3; *significantly different from control).

    Techniques Used: Incubation, MTT Assay

    AFM images of different β-Lg samples incubated at 80°C and pH 2 for 24 h. (A) β-Lg alone, (B) β-Lg in the presence of curcumin, (C) β-Lg in the presence of AFM1 (1 ppb), (D) β-Lg in the presence of AFM1 (1 ppb) and curcumin, (E) β-Lg in the presence of Pb 2+ (0.2 ppm), and (F) β-Lg in the presence of Pb 2+ (0.2 ppm) and curcumin.
    Figure Legend Snippet: AFM images of different β-Lg samples incubated at 80°C and pH 2 for 24 h. (A) β-Lg alone, (B) β-Lg in the presence of curcumin, (C) β-Lg in the presence of AFM1 (1 ppb), (D) β-Lg in the presence of AFM1 (1 ppb) and curcumin, (E) β-Lg in the presence of Pb 2+ (0.2 ppm), and (F) β-Lg in the presence of Pb 2+ (0.2 ppm) and curcumin.

    Techniques Used: Incubation

    ROS production in β-Lg incubated at 80°C and pH 2. (A) In the absence or presence of curcumin and Pb 2+ , and (B) in the absence or presence of curcumin and AFM1. Data represent the means ± SD of 3 independent measurements.
    Figure Legend Snippet: ROS production in β-Lg incubated at 80°C and pH 2. (A) In the absence or presence of curcumin and Pb 2+ , and (B) in the absence or presence of curcumin and AFM1. Data represent the means ± SD of 3 independent measurements.

    Techniques Used: Incubation

    Representative far-UV CD spectra of β-Lg samples after incubation at pH 2 and 80°C. (A) In the absence and presence of Pb 2+ , curcumin and their mixture. (B) In the absence and presence of AFM1, curcumin and their mixture.
    Figure Legend Snippet: Representative far-UV CD spectra of β-Lg samples after incubation at pH 2 and 80°C. (A) In the absence and presence of Pb 2+ , curcumin and their mixture. (B) In the absence and presence of AFM1, curcumin and their mixture.

    Techniques Used: Incubation

    4) Product Images from "A comparative study of procedures for binding of aflatoxin M1 to Lactobacillus rhamnosus GG"

    Article Title: A comparative study of procedures for binding of aflatoxin M1 to Lactobacillus rhamnosus GG

    Journal: Brazilian Journal of Microbiology

    doi: 10.1016/j.bjm.2017.05.003

    Effect of washes on L. rhamnosus GG/AFM1 complex stability. Binding was determined after viable and heat treated bacteria (10 10 CFU) were incubated with AFM1 (1 mL, 50 μg/L) at 37 °C for 18 h with previous pipetting. The complexes formed were exposed to five washes with 1 mL of PBS and the residual AFM1 was quantified. Error bars represent the SD (standard deviation).
    Figure Legend Snippet: Effect of washes on L. rhamnosus GG/AFM1 complex stability. Binding was determined after viable and heat treated bacteria (10 10 CFU) were incubated with AFM1 (1 mL, 50 μg/L) at 37 °C for 18 h with previous pipetting. The complexes formed were exposed to five washes with 1 mL of PBS and the residual AFM1 was quantified. Error bars represent the SD (standard deviation).

    Techniques Used: Binding Assay, Incubation, Standard Deviation

    Effect of washes on L. rhamnosus GG/AFM1 complex stability. Binding was determined after viable and heat treated bacteria (10 10 CFU) were incubated with AFM1 (1 mL, 50 μg/L) at 37 °C for 18 h without previous pipetting. The complexes formed were exposed to five washes with 1 mL of PBS and the residual AFM1 was quantified. Error bars represent the SD.
    Figure Legend Snippet: Effect of washes on L. rhamnosus GG/AFM1 complex stability. Binding was determined after viable and heat treated bacteria (10 10 CFU) were incubated with AFM1 (1 mL, 50 μg/L) at 37 °C for 18 h without previous pipetting. The complexes formed were exposed to five washes with 1 mL of PBS and the residual AFM1 was quantified. Error bars represent the SD.

    Techniques Used: Binding Assay, Incubation

    5) Product Images from "Turn-On Fluorescence Aptasensor on Magnetic Nanobeads for Aflatoxin M1 Detection Based on an Exonuclease III-Assisted Signal Amplification Strategy"

    Article Title: Turn-On Fluorescence Aptasensor on Magnetic Nanobeads for Aflatoxin M1 Detection Based on an Exonuclease III-Assisted Signal Amplification Strategy

    Journal: Nanomaterials

    doi: 10.3390/nano9010104

    Selectivity evaluation of the developed aptasensor for the detection of AFM1 (1 ng mL −1 ) against other control mycotoxins of AFB1, AFM2 and Ochratoxin A at the same concentration.
    Figure Legend Snippet: Selectivity evaluation of the developed aptasensor for the detection of AFM1 (1 ng mL −1 ) against other control mycotoxins of AFB1, AFM2 and Ochratoxin A at the same concentration.

    Techniques Used: Concentration Assay

    Schematic representation of sensitive fluorescent detection of AFM1 based on Exo III-assistant signal amplification recycles.
    Figure Legend Snippet: Schematic representation of sensitive fluorescent detection of AFM1 based on Exo III-assistant signal amplification recycles.

    Techniques Used: Amplification

    The optimization of the experiment: ( a ) Different amount of first-step resultant solution was submitted to the Exo III-assisted recycling amplification in the presence of AFM1. ( n = 3); ( b ) The AFM1 activated first-step displacement time; ( c ) The fluorescence spectra changes according to the concentration of AFM1 (from top to bottom: 20 ng mL −1 to 0 ng mL −1 ); ( d ) The calibration curve was achieved under optimized conditions.
    Figure Legend Snippet: The optimization of the experiment: ( a ) Different amount of first-step resultant solution was submitted to the Exo III-assisted recycling amplification in the presence of AFM1. ( n = 3); ( b ) The AFM1 activated first-step displacement time; ( c ) The fluorescence spectra changes according to the concentration of AFM1 (from top to bottom: 20 ng mL −1 to 0 ng mL −1 ); ( d ) The calibration curve was achieved under optimized conditions.

    Techniques Used: Amplification, Fluorescence, Concentration Assay

    6) Product Images from "Efficient Conjugation of Aflatoxin M1 With Bovine Serum Albumin through Aflatoxin M1-(O-carboxymethyl) Oxime and Production of Anti-aflatoxin M1 Antibodies"

    Article Title: Efficient Conjugation of Aflatoxin M1 With Bovine Serum Albumin through Aflatoxin M1-(O-carboxymethyl) Oxime and Production of Anti-aflatoxin M1 Antibodies

    Journal: Jundishapur Journal of Microbiology

    doi: 10.5812/jjm.8(4)2015.16850

    Consideration of Coupling AFM1 to O-(Carboxymethyl) Hydroxylamine Hemi Hydrochloride by TLC Lane A, standard aflatoxin; lane B, AFM1-(O-carboxymethyl) oxime conjugate.
    Figure Legend Snippet: Consideration of Coupling AFM1 to O-(Carboxymethyl) Hydroxylamine Hemi Hydrochloride by TLC Lane A, standard aflatoxin; lane B, AFM1-(O-carboxymethyl) oxime conjugate.

    Techniques Used: Thin Layer Chromatography

    SDS-PAGE Pattern of Different Fractions of Ion Exchange Chromatography for Purification of IgG Against AFM1 Under Reducing Conditions Followed by Coomassie Blue R250 Staining. Lanes 1 and 2 shows the first peak containing purified antibody, lane 4 and 5 correspond to the second and third peaks respectively. M shows the protein markers with the MW of 94, 66, 45, 30, 20 and 14 KDa from up to down.
    Figure Legend Snippet: SDS-PAGE Pattern of Different Fractions of Ion Exchange Chromatography for Purification of IgG Against AFM1 Under Reducing Conditions Followed by Coomassie Blue R250 Staining. Lanes 1 and 2 shows the first peak containing purified antibody, lane 4 and 5 correspond to the second and third peaks respectively. M shows the protein markers with the MW of 94, 66, 45, 30, 20 and 14 KDa from up to down.

    Techniques Used: SDS Page, Ion Exchange Chromatography, Purification, Staining

    HPLC Chromatogram of Standard AFM1 (A) and AFM1-(O-carboxymethyl) Oxime Derivative (B)
    Figure Legend Snippet: HPLC Chromatogram of Standard AFM1 (A) and AFM1-(O-carboxymethyl) Oxime Derivative (B)

    Techniques Used: High Performance Liquid Chromatography

    UV-VIS Spectrum Obtained From Coupling AFM1-oxime to BSA; Using UV-VIS Spectrophotometry The peaks at wave lengths of 280 nm and 365 nm correspond to BSA and BSA-AFM1 conjugate, respectively.
    Figure Legend Snippet: UV-VIS Spectrum Obtained From Coupling AFM1-oxime to BSA; Using UV-VIS Spectrophotometry The peaks at wave lengths of 280 nm and 365 nm correspond to BSA and BSA-AFM1 conjugate, respectively.

    Techniques Used: Spectrophotometry

    7) Product Images from "Evaluation of Aflatoxin M1 Effects on the Metabolomic and Cytokinomic Profiling of a Hepatoblastoma Cell Line"

    Article Title: Evaluation of Aflatoxin M1 Effects on the Metabolomic and Cytokinomic Profiling of a Hepatoblastoma Cell Line

    Journal: Toxins

    doi: 10.3390/toxins10110436

    ( A ) Cell viability related to HepG2 cells after AFM1 treatment for 48 h. ( B ) Percentage of live, apoptotic, and dead cells (mean ± standard deviation) for HepG2 cells at IC 50 concentration before (CTRL) and after (AFM1) 48 h of treatment. ( C ) Cell percentages in G0/G1, S, and G2/M phases (mean ± standard deviation) for HepG2 cells at IC 50 concentration before (CTRL) and after (AFM1) 48 h of treatment.
    Figure Legend Snippet: ( A ) Cell viability related to HepG2 cells after AFM1 treatment for 48 h. ( B ) Percentage of live, apoptotic, and dead cells (mean ± standard deviation) for HepG2 cells at IC 50 concentration before (CTRL) and after (AFM1) 48 h of treatment. ( C ) Cell percentages in G0/G1, S, and G2/M phases (mean ± standard deviation) for HepG2 cells at IC 50 concentration before (CTRL) and after (AFM1) 48 h of treatment.

    Techniques Used: Standard Deviation, Concentration Assay

    Partial least squares-discriminant analysis (PLS-DA) ( A ) and variable importance in projection (VIP) ( B ) plots related to the polar fraction of the HepG2 cell line treated with AFM1 compared to untreated cells.
    Figure Legend Snippet: Partial least squares-discriminant analysis (PLS-DA) ( A ) and variable importance in projection (VIP) ( B ) plots related to the polar fraction of the HepG2 cell line treated with AFM1 compared to untreated cells.

    Techniques Used:

    8) Product Images from "Calcium montmorillonite clay reduces AFB1 and FB1 biomarkers in rats exposed to single and co-exposures of aflatoxin and fumonisin"

    Article Title: Calcium montmorillonite clay reduces AFB1 and FB1 biomarkers in rats exposed to single and co-exposures of aflatoxin and fumonisin

    Journal: Journal of applied toxicology : JAT

    doi: 10.1002/jat.2942

    Mean excretion pattern of AFM1. (A) 0.125 mg AFB1 per kg body weight-treated groups with 0, 0.25 and 2% UPSN. (B) Table of percent reduction between the AFB1-treated positive control group (no clay) and each AFB1/UPSN group at time points 12, 24 and 36
    Figure Legend Snippet: Mean excretion pattern of AFM1. (A) 0.125 mg AFB1 per kg body weight-treated groups with 0, 0.25 and 2% UPSN. (B) Table of percent reduction between the AFB1-treated positive control group (no clay) and each AFB1/UPSN group at time points 12, 24 and 36

    Techniques Used: Positive Control

    (A) Area under the curve (AUC) for pharmacokinetic excretion of AFM1 in urine. The black bars are indicative of total AUC for rats treated with 0.125 mg AFB1 per kg body weight and 0, 0.25 or 2% UPSN. Gray bars are indicative of total AUC for rats treated
    Figure Legend Snippet: (A) Area under the curve (AUC) for pharmacokinetic excretion of AFM1 in urine. The black bars are indicative of total AUC for rats treated with 0.125 mg AFB1 per kg body weight and 0, 0.25 or 2% UPSN. Gray bars are indicative of total AUC for rats treated

    Techniques Used:

    9) Product Images from "Efficient Conjugation of Aflatoxin M1 With Bovine Serum Albumin through Aflatoxin M1-(O-carboxymethyl) Oxime and Production of Anti-aflatoxin M1 Antibodies"

    Article Title: Efficient Conjugation of Aflatoxin M1 With Bovine Serum Albumin through Aflatoxin M1-(O-carboxymethyl) Oxime and Production of Anti-aflatoxin M1 Antibodies

    Journal: Jundishapur Journal of Microbiology

    doi: 10.5812/jjm.8(4)2015.16850

    Consideration of Coupling AFM1 to O-(Carboxymethyl) Hydroxylamine Hemi Hydrochloride by TLC Lane A, standard aflatoxin; lane B, AFM1-(O-carboxymethyl) oxime conjugate.
    Figure Legend Snippet: Consideration of Coupling AFM1 to O-(Carboxymethyl) Hydroxylamine Hemi Hydrochloride by TLC Lane A, standard aflatoxin; lane B, AFM1-(O-carboxymethyl) oxime conjugate.

    Techniques Used: Thin Layer Chromatography

    SDS-PAGE Pattern of Different Fractions of Ion Exchange Chromatography for Purification of IgG Against AFM1 Under Reducing Conditions Followed by Coomassie Blue R250 Staining. Lanes 1 and 2 shows the first peak containing purified antibody, lane 4 and 5 correspond to the second and third peaks respectively. M shows the protein markers with the MW of 94, 66, 45, 30, 20 and 14 KDa from up to down.
    Figure Legend Snippet: SDS-PAGE Pattern of Different Fractions of Ion Exchange Chromatography for Purification of IgG Against AFM1 Under Reducing Conditions Followed by Coomassie Blue R250 Staining. Lanes 1 and 2 shows the first peak containing purified antibody, lane 4 and 5 correspond to the second and third peaks respectively. M shows the protein markers with the MW of 94, 66, 45, 30, 20 and 14 KDa from up to down.

    Techniques Used: SDS Page, Ion Exchange Chromatography, Purification, Staining

    HPLC Chromatogram of Standard AFM1 (A) and AFM1-(O-carboxymethyl) Oxime Derivative (B)
    Figure Legend Snippet: HPLC Chromatogram of Standard AFM1 (A) and AFM1-(O-carboxymethyl) Oxime Derivative (B)

    Techniques Used: High Performance Liquid Chromatography

    UV-VIS Spectrum Obtained From Coupling AFM1-oxime to BSA; Using UV-VIS Spectrophotometry The peaks at wave lengths of 280 nm and 365 nm correspond to BSA and BSA-AFM1 conjugate, respectively.
    Figure Legend Snippet: UV-VIS Spectrum Obtained From Coupling AFM1-oxime to BSA; Using UV-VIS Spectrophotometry The peaks at wave lengths of 280 nm and 365 nm correspond to BSA and BSA-AFM1 conjugate, respectively.

    Techniques Used: Spectrophotometry

    10) Product Images from "Development of a UPLC-FLD Method for Detection of Aflatoxin B1 and M1 in Animal Tissue to Study the Effect of Curcumin on Mycotoxin Clearance Rates"

    Article Title: Development of a UPLC-FLD Method for Detection of Aflatoxin B1 and M1 in Animal Tissue to Study the Effect of Curcumin on Mycotoxin Clearance Rates

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2017.00650

    The representative semi-logarithmic plot shows aflatoxin B1 (AFB1) and aflatoxin M1 (AFM1) residue concentrations for broiler kidney tissues vs. time , with the one-sided 95% upper tolerance limit in AFB1-fed group; (A) AFB1 residues (C) AFM1 residues and in curcumin + AFB1-fed group; (B) AFB1 residues (D) AFM1 residues. Small circles represent the residue concentrations of AFB1 and AFM1 for individual broiler chicken. The clearance time calculation based on the limit of quantification (LOQ; 0.02 μg/kg for AFB1 and 0.01 μg/kg for AFM1).
    Figure Legend Snippet: The representative semi-logarithmic plot shows aflatoxin B1 (AFB1) and aflatoxin M1 (AFM1) residue concentrations for broiler kidney tissues vs. time , with the one-sided 95% upper tolerance limit in AFB1-fed group; (A) AFB1 residues (C) AFM1 residues and in curcumin + AFB1-fed group; (B) AFB1 residues (D) AFM1 residues. Small circles represent the residue concentrations of AFB1 and AFM1 for individual broiler chicken. The clearance time calculation based on the limit of quantification (LOQ; 0.02 μg/kg for AFB1 and 0.01 μg/kg for AFM1).

    Techniques Used:

    The representative semi-logarithmic plot shows aflatoxin B1 (AFB1) and aflatoxin M1 (AFM1) residue concentrations for broiler liver tissues vs. time , with the one-sided 95% upper tolerance limit in AFB1-fed group; (A) AFB1 residues (C) AFM1 residues and in curcumin + AFB1-fed group; (B) AFB1 residues (D) AFM1 residues. Small circles represent the residue concentrations of AFB1 and AFM1 for individual broiler chickens. The clearance period calculation based on the limit of quantification (LOQ; 0.02 μg/kg for AFB1 and 0.01 μg/kg for AFM1).
    Figure Legend Snippet: The representative semi-logarithmic plot shows aflatoxin B1 (AFB1) and aflatoxin M1 (AFM1) residue concentrations for broiler liver tissues vs. time , with the one-sided 95% upper tolerance limit in AFB1-fed group; (A) AFB1 residues (C) AFM1 residues and in curcumin + AFB1-fed group; (B) AFB1 residues (D) AFM1 residues. Small circles represent the residue concentrations of AFB1 and AFM1 for individual broiler chickens. The clearance period calculation based on the limit of quantification (LOQ; 0.02 μg/kg for AFB1 and 0.01 μg/kg for AFM1).

    Techniques Used:

    The representative semi-logarithmic plot shows aflatoxin B1 (AFB1) and aflatoxin M1 (AFM1) residue concentrations for broiler muscles tissues vs. time , with the one-sided 95% upper tolerance limit in AFB1-fed group; (A) AFB1 residues (C) AFM1 residues and in curcumin + AFB1-fed group; (B) AFB1 residues (D) AFM1 residues. Small circles represent the residue concentrations of AFB1 and AFM1 for individual broiler chicken. The clearance period calculation based on the limit of quantification (LOQ; 0.02 μg/kg for AFB1 and 0.01 μg/kg for AFM1).
    Figure Legend Snippet: The representative semi-logarithmic plot shows aflatoxin B1 (AFB1) and aflatoxin M1 (AFM1) residue concentrations for broiler muscles tissues vs. time , with the one-sided 95% upper tolerance limit in AFB1-fed group; (A) AFB1 residues (C) AFM1 residues and in curcumin + AFB1-fed group; (B) AFB1 residues (D) AFM1 residues. Small circles represent the residue concentrations of AFB1 and AFM1 for individual broiler chicken. The clearance period calculation based on the limit of quantification (LOQ; 0.02 μg/kg for AFB1 and 0.01 μg/kg for AFM1).

    Techniques Used:

    11) Product Images from "Palladium Nanoparticles-Based Fluorescence Resonance Energy Transfer Aptasensor for Highly Sensitive Detection of Aflatoxin M1 in Milk"

    Article Title: Palladium Nanoparticles-Based Fluorescence Resonance Energy Transfer Aptasensor for Highly Sensitive Detection of Aflatoxin M1 in Milk

    Journal: Toxins

    doi: 10.3390/toxins9100318

    Relative fluorescence intensity (( F other mycotoxins − F 0 )/( F AFM1 − F 0 )) of the aptamer-bridged FRET biosensor for AFM 1 detection in the presence of different mycotoxins, where F 0 is the fluorescence intensity in the absence of AFM 1 or other mycotoxins. Data were presented as average ±SD from three independent measurements. The concentration of mycotoxins were all 150 pg/mL. Experiments were conducted in HEPES buffer under excitation at 480 nm.
    Figure Legend Snippet: Relative fluorescence intensity (( F other mycotoxins − F 0 )/( F AFM1 − F 0 )) of the aptamer-bridged FRET biosensor for AFM 1 detection in the presence of different mycotoxins, where F 0 is the fluorescence intensity in the absence of AFM 1 or other mycotoxins. Data were presented as average ±SD from three independent measurements. The concentration of mycotoxins were all 150 pg/mL. Experiments were conducted in HEPES buffer under excitation at 480 nm.

    Techniques Used: Fluorescence, Concentration Assay

    Related Articles

    Modification:

    Article Title: Curcumin Protects β-Lactoglobulin Fibril Formation and Fibril-Induced Neurotoxicity in PC12Cells
    Article Snippet: .. Materials Bovine β-Lg type A (L7870),Curcumin (C-1386), thioflavin-T (ThT; T-3516), AFM1, Nerve growth factor (NGF) and Dulbecco’s modified Eagle’s medium (DMEM) were purchased from Sigma-Aldrich (USA). .. Fetal bovine serum (FBS) and the horse serum were obtained from Gibco (Life Technologies, USA) and Rat pheochromocytoma PC12 cells from Pasture Institute of Iran (Tehran, Iran) was used.

    Concentration Assay:

    Article Title: Evaluation of Aflatoxin M1 Effects on the Metabolomic and Cytokinomic Profiling of a Hepatoblastoma Cell Line
    Article Snippet: .. A stock solution of AFM1 at a concentration of 1 mM was prepared in dimethyl sulfoxide (DMSO Sigma-Aldrich, St. Louis, MO, USA) arriving to a final DMSO concentration of lower than 0.1% by serial dilutions. .. A total of 2 × 103 cells per well were plated and allowed to attach for 24 h. Cells were then stimulated with 0.1, 1, 5, 10, 25, and 50 µM concentrations of AFM1 (this concentration range was selected on the basis of the literature [ , ]).

    Binding Assay:

    Article Title: A comparative study of procedures for binding of aflatoxin M1 to Lactobacillus rhamnosus GG
    Article Snippet: .. AFM1 binding assay A standard of AFM1 (10 μg/mL), suspended in acetonitrile, was obtained from Sigma (St. Louis, MO, USA). .. To prepare solutions A and B with a concentration of 100 μg/L and 50 μg/L, respectively, the AFM1 stock solution was diluted with phosphate-buffered saline (PBS; pH 7.4, 0.05% Tween 20, v/v).

    Construct:

    Article Title: A comparative study of procedures for binding of aflatoxin M1 to Lactobacillus rhamnosus GG
    Article Snippet: .. The AFM1 retention time was 7.5 min. A calibration curve was constructed with AFM1 standard (Sigma–Aldrich, MO, USA) at concentrations ranging from 0.1 to 100 μg/L. .. Two-way ANOVA was conducted using SPSS 19.0 statistical software (SPSS Inc., Chicago, IL, USA) to identify significant differences between the different procedures.

    Conjugation Assay:

    Article Title: Efficient Conjugation of Aflatoxin M1 With Bovine Serum Albumin through Aflatoxin M1-(O-carboxymethyl) Oxime and Production of Anti-aflatoxin M1 Antibodies
    Article Snippet: .. Conjugation of AFM1-(O-carboxymethyl) Oxime to Bovine Serum Albumin One hundred microliter of AFM1 -oxime in 400 μL of 25% ethanol containing 6.5 mg EDS was mixed with 200 μg of BSA (Sigma, St. Louis, MO, USA) in 20 μL of 0.05 M sodium phosphate buffer (pH 7.2). ..

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    Millipore millex
    Millex, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/millex/product/Millipore
    Average 99 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    millex - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Millipore ptfe
    Ptfe, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ptfe/product/Millipore
    Average 99 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    ptfe - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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