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SouthernBiotech af555
Af555, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/af555/Goat+Anti-Mouse+IgG%2C+Human+ads-Alexa+Fluor+555/pm42492349-98-34-35
Average 93 stars, based on 9 article reviews
af555 - by Bioz Stars, 2026-10
93/100 stars

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Article Title: Human iPSC-derived neural stem cells engraft and improve pathophysiology of MPS I mice
Article Snippet: Goat anti-mouse , IgG(H + L) , AF555 , SouthernBiotech, 1031-32 , AB_2794317 , IF , 1:250.

Incubation:

Article Title: Crystalline silica exposure of genetically diverse mice exacerbates subclinical autoimmunity into systemic autoimmune disease phenotypes including inflammatory arthritis in female mice.
Article Snippet: Inhalation of respirable crystalline silica (c-silica), an occupational hazard, has been associated with the development of systemic autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosus.. However, it is unclear if c-silica-mediated autoimmune disease requires pre-existing subclinical autoimmunity or to what extent the severity of existing subclinical autoimmunity influences the spectrum and severity of c-silicainduced autoimmune phenotypes.. Sixty-one naïve Collaborative Cross (CC) strains, a multiparental panel of recombinant inbred (RI) mouse strains derived from eight founder strains, were assessed for the presence of subclinical autoimmunity via autoantibodies and inflammatory markers.

Article Title: Crystalline silica exposure induces multiple systemic autoimmune phenotypes including inflammatory arthritis and nephritis in Collaborative Cross mice with differing sub-clinical autoimmune profiles
Article Snippet: .. Slides were incubated with ice cold acetone (100%, 10 min), washed in PBS, and blocked in 2% BSA for 45 min. before overnight incubation at 4°C with an antibody-cocktail containing AF555-conjugated-anti-IgG (Southern Biotech, Catalog #: 1030-32) diluted 1/50 and FITC-conjugated anti-C3 (ICL Lab, Catalog#: GC3-90F-Z) diluted 1/100. .. Slides were then washed with PBS, and coverslips mounted using Vectashield anti-fade mounting medium with DAPI (Vector Laboratories Inc., Ref. H-1200).



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