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adenosine 5  (MedChemExpress)


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    MedChemExpress adenosine 5
    Adenosine 5, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adenosine 5/product/MedChemExpress
    Average 94 stars, based on 12 article reviews
    adenosine 5 - by Bioz Stars, 2026-02
    94/100 stars

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    Rapid priming sensitizes the NAIP–NLRC4 inflammasome in a p38-dependent manner. ( A and B ) WT or NLRC4 –/– THP-1 cells primed for 1 h with Pam3CSK4 (P3) or BS103 at MOI 10 (BS) before challenge with PA or PA+LFn-Needle. Cell death collected at 1 hpc ( A ), and 3 hpc ( B ). ( C ) Western Blot of lysates from GSDMD –/– THP-1 cells primed for 1 h with P3 or Left unprimed before challenge with PA, PA+100 ng/mL LFn-Needle, or Nigericin, collected at 3 hpc. ( D ) WT THP-1 cells pretreated for 1 h with DMSO or PD169316 , and primed for 1 h <t>with</t> <t>P3,</t> <t>ADP-L-Heptose</t> (AH), or BS at MOI 10 before challenge with PA, PA+LFn-Needle, or Nigericin. Data shown are from one experiment, which are representative of more than three independent experiments. Individual data points represent technical replicates. Cell death was measured at 1 hpc or 3 hpc by PI uptake and calculated as % Cell Death relative to TritonX-100 treatment. Data represent the mean ± SD. Two-way ANOVA. * P < 0.0332, ** P < 0.0021, *** P < 0.0002, **** P < 0.0001.
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    adenosine diphosphate  (MedChemExpress)
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    MedChemExpress adenosine diphosphate
    Rapid priming sensitizes the NAIP–NLRC4 inflammasome in a p38-dependent manner. ( A and B ) WT or NLRC4 –/– THP-1 cells primed for 1 h with Pam3CSK4 (P3) or BS103 at MOI 10 (BS) before challenge with PA or PA+LFn-Needle. Cell death collected at 1 hpc ( A ), and 3 hpc ( B ). ( C ) Western Blot of lysates from GSDMD –/– THP-1 cells primed for 1 h with P3 or Left unprimed before challenge with PA, PA+100 ng/mL LFn-Needle, or Nigericin, collected at 3 hpc. ( D ) WT THP-1 cells pretreated for 1 h with DMSO or PD169316 , and primed for 1 h <t>with</t> <t>P3,</t> <t>ADP-L-Heptose</t> (AH), or BS at MOI 10 before challenge with PA, PA+LFn-Needle, or Nigericin. Data shown are from one experiment, which are representative of more than three independent experiments. Individual data points represent technical replicates. Cell death was measured at 1 hpc or 3 hpc by PI uptake and calculated as % Cell Death relative to TritonX-100 treatment. Data represent the mean ± SD. Two-way ANOVA. * P < 0.0332, ** P < 0.0021, *** P < 0.0002, **** P < 0.0001.
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    Image Search Results


    Rapid priming sensitizes the NAIP–NLRC4 inflammasome in a p38-dependent manner. ( A and B ) WT or NLRC4 –/– THP-1 cells primed for 1 h with Pam3CSK4 (P3) or BS103 at MOI 10 (BS) before challenge with PA or PA+LFn-Needle. Cell death collected at 1 hpc ( A ), and 3 hpc ( B ). ( C ) Western Blot of lysates from GSDMD –/– THP-1 cells primed for 1 h with P3 or Left unprimed before challenge with PA, PA+100 ng/mL LFn-Needle, or Nigericin, collected at 3 hpc. ( D ) WT THP-1 cells pretreated for 1 h with DMSO or PD169316 , and primed for 1 h with P3, ADP-L-Heptose (AH), or BS at MOI 10 before challenge with PA, PA+LFn-Needle, or Nigericin. Data shown are from one experiment, which are representative of more than three independent experiments. Individual data points represent technical replicates. Cell death was measured at 1 hpc or 3 hpc by PI uptake and calculated as % Cell Death relative to TritonX-100 treatment. Data represent the mean ± SD. Two-way ANOVA. * P < 0.0332, ** P < 0.0021, *** P < 0.0002, **** P < 0.0001.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Shigella OspF blocks rapid p38-dependent priming of the NAIP–NLRC4 inflammasome

    doi: 10.1073/pnas.2510950123

    Figure Lengend Snippet: Rapid priming sensitizes the NAIP–NLRC4 inflammasome in a p38-dependent manner. ( A and B ) WT or NLRC4 –/– THP-1 cells primed for 1 h with Pam3CSK4 (P3) or BS103 at MOI 10 (BS) before challenge with PA or PA+LFn-Needle. Cell death collected at 1 hpc ( A ), and 3 hpc ( B ). ( C ) Western Blot of lysates from GSDMD –/– THP-1 cells primed for 1 h with P3 or Left unprimed before challenge with PA, PA+100 ng/mL LFn-Needle, or Nigericin, collected at 3 hpc. ( D ) WT THP-1 cells pretreated for 1 h with DMSO or PD169316 , and primed for 1 h with P3, ADP-L-Heptose (AH), or BS at MOI 10 before challenge with PA, PA+LFn-Needle, or Nigericin. Data shown are from one experiment, which are representative of more than three independent experiments. Individual data points represent technical replicates. Cell death was measured at 1 hpc or 3 hpc by PI uptake and calculated as % Cell Death relative to TritonX-100 treatment. Data represent the mean ± SD. Two-way ANOVA. * P < 0.0332, ** P < 0.0021, *** P < 0.0002, **** P < 0.0001.

    Article Snippet: For activating PRRs, cells were treated with 100 ng/mL Pam3CSK4 (Invivogen, tlrl-pms), 100 ng/mL ADP-L-Heptose (Invivogen, tlrl-adph-l), 100 ng/mL LPS (Enzo, ALX-581-013), or 1 μg/mL C12-iE-DAP (Invivogen, tlrl-c12dap) for the indicated times.

    Techniques: Western Blot