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acrylamide bis crosslinking (Bio-Rad)

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Bio-Rad acrylamide bis crosslinking
Acrylamide Bis Crosslinking, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 10173 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/acrylamide/Acrylamide/10__22175_slash_mmb__15701-88-55-93
Average 97 stars, based on 10173 article reviews

acrylamide bis crosslinking - by Bioz Stars, 2026-09

97/100 stars

Images

Staining:

Article Title: Biochemical characterisation and substrate-specific proteolytic diversity of venom metalloproteinases in African puff adders
Article Snippet: .. The gels are 4-20% acrylamide (BioRad) and were stained using Coomassie Blue R250 (prothrombin) or silver stain (fibrinogen and factor X). ..

Article Title: Biochemical characterisation and substrate-specific proteolytic diversity of venom metalloproteinases in African puff adders
Article Snippet: .. The gel used was 4-20% acrylamide (BioRad) and was stained with Coomassie Blue R250. .. Lane 1, casein control; lanes 2 (no EDTA) and 3 (EDTA) KEN 26 kDa SVMP; lanes 4 (no EDTA) and 5 (EDTA) KEN 68 kDa SVMP; lanes 6 (no EDTA) and 7 (EDTA) NGA 30 kDa SVMP; lanes 8 (no EDTA) and 9 (EDTA) TZA 21 kDa SVMP; lane M, molecular weight markers (Thermo Page Ruler), indicated in kDa.

Article Title: Biochemical characterisation and substrate-specific proteolytic diversity of venom metalloproteinases in African puff adders
Article Snippet: Lane 1, standard reducing conditions; lane M, molecular weight markers (Thermo PageRuler with key molecular weights indicated in kDa), lane 2 non-reducing conditions (0.5% SDS), lane 3 as lane 2 but with 0.25% NP40 added after the SDS, lane 4, as lane 2 but with 0.25% NP40 added prior to the SDS. .. The gel used was a 4-20% acrylamide (BioRad) and was stained with Coomassie Blue R250. ..

Silver Staining:

Article Title: Biochemical characterisation and substrate-specific proteolytic diversity of venom metalloproteinases in African puff adders
Article Snippet: .. The gels are 4-20% acrylamide (BioRad) and were stained using Coomassie Blue R250 (prothrombin) or silver stain (fibrinogen and factor X). ..

Incubation:

Article Title: Altair-dvOPM: an open-access platform for large-field three-dimensional tissue imaging
Article Snippet: .. Fixed cells were rinsed three times with 1 × PBS to remove NaN 3 and incubated in an anchoring solution containing 1.4% formaldehyde (FA, Sigma-Aldrich) and 2% acrylamide (AA, Bio-Rad) in 1 × PBS for 3 h at 37 °C without agitation. .. Following anchoring, samples were incubated in monomer solution consisting of 23% sodium acrylate (SA, AmBeed), 10% AA, 0.1% N,N′-methylenbisacrylamide (BIS, Sigma-Aldrich), 0.5% tetramethylethylenediamine (TEMED, Bio-Rad), 1 × PBS and Milli-Q water for 30 min on ice to allow uniform monomer diffusion.