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25922 ab9 herrera espejo et  (ATCC)


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    ATCC 25922 ab9 herrera espejo et
    25922 Ab9 Herrera Espejo Et, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ab9/pm41174184-252-74-73?v=ATCC
    Average 94 stars, based on 53 article reviews
    25922 ab9 herrera espejo et - by Bioz Stars, 2026-07
    94/100 stars

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    Image Search Results


    (a) Thermogram for the ITC titration of 20 μM Neu5Ac aptamer titrated by 1 mM Neu5Ac in aptamer binding buffer; (b) Integrated heat of the ITC titration for Neu5Ac aptamer and Neu5Ac, the black line represents the binding curve fitted with the ‘one set of binding sites’ model; (c) Blotting of total RNA from HeLa cells after metabolic labeling with Ac4ManNAz, or HeLa cells without metabolic labeling; (d) ARPLA-mediated glycoRNA imaging on the surface of HeLa, HL-60, and THP-1 cells. Scale bar: 20 μm (HeLa), 10 μm (HL-60, THP-1); (e) Transmission-through dye microscopic image of HeLa cell. The membrane permeable dye (CellTracker Orange CMRA) and the membrane impermeant quencher acid blue 9 (AB9) were applied to the same cells. AB9 cannot enter the cell with intact membrane and thus cannot quench the membrane permeable dye, so the cells with intact membrane show bright fluorescence signals from CellTracker Orange CMRA. Leaky or damaged membrane after permeabilization treatment allows for the quencher to enter the cell, resulting in reduced or diminished fluorescence of the cell. Scale bar: 40 μm. All experiments were repeated independently three times with similar results.

    Journal: Nature biotechnology

    Article Title: Spatial imaging of glycoRNA in single cells with ARPLA

    doi: 10.1038/s41587-023-01801-z

    Figure Lengend Snippet: (a) Thermogram for the ITC titration of 20 μM Neu5Ac aptamer titrated by 1 mM Neu5Ac in aptamer binding buffer; (b) Integrated heat of the ITC titration for Neu5Ac aptamer and Neu5Ac, the black line represents the binding curve fitted with the ‘one set of binding sites’ model; (c) Blotting of total RNA from HeLa cells after metabolic labeling with Ac4ManNAz, or HeLa cells without metabolic labeling; (d) ARPLA-mediated glycoRNA imaging on the surface of HeLa, HL-60, and THP-1 cells. Scale bar: 20 μm (HeLa), 10 μm (HL-60, THP-1); (e) Transmission-through dye microscopic image of HeLa cell. The membrane permeable dye (CellTracker Orange CMRA) and the membrane impermeant quencher acid blue 9 (AB9) were applied to the same cells. AB9 cannot enter the cell with intact membrane and thus cannot quench the membrane permeable dye, so the cells with intact membrane show bright fluorescence signals from CellTracker Orange CMRA. Leaky or damaged membrane after permeabilization treatment allows for the quencher to enter the cell, resulting in reduced or diminished fluorescence of the cell. Scale bar: 40 μm. All experiments were repeated independently three times with similar results.

    Article Snippet: Acid Blue 9 (AB9) and a Minute plasma membrane-derived lipid raft isolation kit were purchased from Fisher Scientific.

    Techniques: Titration, Binding Assay, Labeling, Imaging, Transmission Assay, Membrane, Fluorescence