ab5075  (ATCC)

Journal: Antibiotics

Article Title: GATR-3, a Peptide That Eradicates Preformed Biofilms of Multidrug-Resistant Acinetobacter baumannii

doi: 10.3390/antibiotics13010039

Figure Lengend Snippet: Minimum inhibitory concentration of GATR-3 peptide against a collection of MDR A. baumannii strains and other ESKAPE pathogens. The MIC is indicated in μg/mL. We followed the protocol for CLSI testing with the adaptation for peptides [ 28 ].

Article Snippet: AB5075 (MRSN 959) was obtained from BEI Resources (NR-52248, Manassas, VA, USA), and it is a part of the MDR diversity panel.

Techniques: Concentration Assay

Journal: Antibiotics

Article Title: GATR-3, a Peptide That Eradicates Preformed Biofilms of Multidrug-Resistant Acinetobacter baumannii

doi: 10.3390/antibiotics13010039

Figure Lengend Snippet: Estimated MBIC 50 of GATR-3, LL-37, IDR-1018 and polymyxin B against MDR A. baumannii strains.

Article Snippet: AB5075 (MRSN 959) was obtained from BEI Resources (NR-52248, Manassas, VA, USA), and it is a part of the MDR diversity panel.

Techniques:

Journal: Antibiotics

Article Title: GATR-3, a Peptide That Eradicates Preformed Biofilms of Multidrug-Resistant Acinetobacter baumannii

doi: 10.3390/antibiotics13010039

Figure Lengend Snippet: MBEC 50 of peptides and antibiotics against tested bacteria.

Article Snippet: AB5075 (MRSN 959) was obtained from BEI Resources (NR-52248, Manassas, VA, USA), and it is a part of the MDR diversity panel.

Techniques: Bacteria

Journal: Antibiotics

Article Title: GATR-3, a Peptide That Eradicates Preformed Biofilms of Multidrug-Resistant Acinetobacter baumannii

doi: 10.3390/antibiotics13010039

Figure Lengend Snippet: Log reduction in CFUs of AB5075 using a minimum biofilm eradication concentration (MBEC) device. Preformed biofilms of AB5075 were challenged with ( A ) GATR-3 and ( B ) polymyxin B ( n = 3), which resulted in complete eradication of the bacteria at 32 and 2 µg/mL, respectively. The figures represent the number of bacteria present in biofilms at each tested concentration. The detailed mean and standard deviation values can be found in . ( C ) Schematic diagram of samples collected for SEM imaging. ( D – F ) Scanning electron micrographs of the A. baumannii AB5075 biofilm. Bacteria were grown on pegs using an MBEC device to establish biofilms. After incubation, biofilms were treated with 32 µg/mL GATR-3. ( D ) Untreated bacteria (white arrows indicate the slimy material) and ( E ) GATR-3-treated bacteria (white arrows indicate dead cells) were retrieved after sonication of the pegs. ( F ) GATR-3-treated bacteria in the treatment well (detached from the pegs before sonication). Images are taken at 15,000× with a working distance of 9.8 mm. The white bar represents 1 µm.

Article Snippet: AB5075 (MRSN 959) was obtained from BEI Resources (NR-52248, Manassas, VA, USA), and it is a part of the MDR diversity panel.

Techniques: Concentration Assay, Bacteria, Standard Deviation, Imaging, Incubation, Sonication

Journal: Antibiotics

Article Title: GATR-3, a Peptide That Eradicates Preformed Biofilms of Multidrug-Resistant Acinetobacter baumannii

doi: 10.3390/antibiotics13010039

Figure Lengend Snippet: Time to Kill and Resistance of GATR-3 peptide. ( A ) Time-kill kinetics of GATR-3 against A. baumannii AB5075 upon exposure to 1-, 5- and 10-fold the MIC (4 µg/mL) over 10, 30, 60, and 180 min. “MIC ×” indicates the multiple of MIC used. ( B ) Resistance acquisition of AB5075 to GATR-3 and colistin was performed upon exposure to subinhibitory concentrations of the challenge.

Article Snippet: AB5075 (MRSN 959) was obtained from BEI Resources (NR-52248, Manassas, VA, USA), and it is a part of the MDR diversity panel.

Techniques:

Journal: Antibiotics

Article Title: GATR-3, a Peptide That Eradicates Preformed Biofilms of Multidrug-Resistant Acinetobacter baumannii

doi: 10.3390/antibiotics13010039

Figure Lengend Snippet: Membrane effects of GATR-3 on MDR A. baumannii AB5075. ( A ) Membrane depolarization by peptides GATR-3, LL-37, and polymyxin B (PMB) as assessed by the fluorescent dye DiSC 3 (5). ( B ) Membrane disruption (pore formation) was also assessed by ethidium bromide. Bacteria were incubated with 50 µg/mL peptide/drug, and the results were obtained after 20 min of challenge exposure. Student’s t -test was performed on untreated against treated bacteria (*** p < 0.001, **** p < 0.0001). The experiment was repeated twice, and one representative experiment was chosen.

Article Snippet: AB5075 (MRSN 959) was obtained from BEI Resources (NR-52248, Manassas, VA, USA), and it is a part of the MDR diversity panel.

Techniques: Membrane, Disruption, Bacteria, Incubation

Journal: Antibiotics

Article Title: Sensitization of Gram-Negative Bacteria to Aminoglycosides with 2-Aminoimidazole Adjuvants

doi: 10.3390/antibiotics12111563

Figure Lengend Snippet: TOB MICs in the presence of compounds 1 – 5 against AB5075 and AB19606.

Article Snippet: CosmosID provided an Excel document with a list of genetic differences between the parent and evolved AB5075 strains and an annotated AB5075 reference (gabab5075v1.0: https://www.ncbi.nlm.nih.gov/assembly/GCF_000770605.1 , accessed on 17 October 2022).

Techniques: Concentration Assay

Journal: Antibiotics

Article Title: Sensitization of Gram-Negative Bacteria to Aminoglycosides with 2-Aminoimidazole Adjuvants

doi: 10.3390/antibiotics12111563

Figure Lengend Snippet: TOB MICs in the presence of compounds 6 – 12 against AB5075 and AB19606.

Article Snippet: CosmosID provided an Excel document with a list of genetic differences between the parent and evolved AB5075 strains and an annotated AB5075 reference (gabab5075v1.0: https://www.ncbi.nlm.nih.gov/assembly/GCF_000770605.1 , accessed on 17 October 2022).

Techniques: Concentration Assay

Journal: Antibiotics

Article Title: Sensitization of Gram-Negative Bacteria to Aminoglycosides with 2-Aminoimidazole Adjuvants

doi: 10.3390/antibiotics12111563

Figure Lengend Snippet: Additional aminoglycoside MICs in the presence of select 2-AI compounds against AB5075.

Article Snippet: CosmosID provided an Excel document with a list of genetic differences between the parent and evolved AB5075 strains and an annotated AB5075 reference (gabab5075v1.0: https://www.ncbi.nlm.nih.gov/assembly/GCF_000770605.1 , accessed on 17 October 2022).

Techniques: Concentration Assay

Journal: Antibiotics

Article Title: Sensitization of Gram-Negative Bacteria to Aminoglycosides with 2-Aminoimidazole Adjuvants

doi: 10.3390/antibiotics12111563

Figure Lengend Snippet: Tobramycin MICs (μg/mL) of serially passaged bacterial populations.

Article Snippet: CosmosID provided an Excel document with a list of genetic differences between the parent and evolved AB5075 strains and an annotated AB5075 reference (gabab5075v1.0: https://www.ncbi.nlm.nih.gov/assembly/GCF_000770605.1 , accessed on 17 October 2022).

Techniques: