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a8301  (MedChemExpress)


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    MedChemExpress a8301
    A8301, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/a8301/A+83-01/pm41963291-187-39-40
    Average 97 stars, based on 110 article reviews
    a8301 - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Suspension:

    Article Title: Colon-Targeted astragalus polysaccharide nanoparticles prevent NAFLD-Driven hepatocarcinogenesis via microbiota remodeling and NF-κB Inhibition.
    Article Snippet: .. The resulting suspension was embedded in Matrigel (356234, Corning, USA) and cultured in DMEM/F12 medium (11320033, Thermo Fisher, USA) supplemented with 100 U/mL penicillin–streptomycin, 10 mM HEPES (15630106, Thermo Fisher), GlutaMAX (35050061, AR TIC LE IN PR ES S Thermo Fisher), B27 (C0347, Beyotime), N2 (17502048, Thermo Fisher), 30% Wnt3a, 10% R-spondin, 10% murine Noggin (conditioned media), 10 μM forskolin (F6886, Sigma), 100 ng/mL murine EGF (SRP3196, Sigma), 100 ng/mL FGF10 (GF172, Sigma), 25 ng/mL HGF (SRP6014, Sigma), 10 nM gastrin I (HY-P1097, MCE), 1.25 mM N-acetylcysteine (50303ES05, Yeasen, China), 10 mM nicotinamide (72340, Sigma), 5 μM A8301 (53002ES03, Yeasen), and 10 μM Y27632 (HY-10071, MCE). ..

    Article Title: Colon-Targeted astragalus polysaccharide nanoparticles prevent NAFLD-Driven hepatocarcinogenesis via microbiota remodeling and NF-κB Inhibition
    Article Snippet: .. The resulting suspension was embedded in Matrigel (356234, Corning, USA) and cultured in DMEM/F12 medium (11320033, Thermo Fisher, USA) supplemented with 100 U/mL penicillin–streptomycin, 10 mM HEPES (15630106, Thermo Fisher), GlutaMAX (35050061, Thermo Fisher), B27 (C0347, Beyotime), N2 (17502048, Thermo Fisher), 30% Wnt3a, 10% R-spondin, 10% murine Noggin (conditioned media), 10 μM forskolin (F6886, Sigma), 100 ng/mL murine EGF (SRP3196, Sigma), 100 ng/mL FGF10 (GF172, Sigma), 25 ng/mL HGF (SRP6014, Sigma), 10 nM gastrin I (HY-P1097, MCE), 1.25 mM N-acetylcysteine (50303ES05, Yeasen, China), 10 mM nicotinamide (72340, Sigma), 5 μM A8301 (53002ES03, Yeasen), and 10 μM Y27632 (HY-10071, MCE). ..

    Cell Culture:

    Article Title: Colon-Targeted astragalus polysaccharide nanoparticles prevent NAFLD-Driven hepatocarcinogenesis via microbiota remodeling and NF-κB Inhibition.
    Article Snippet: .. The resulting suspension was embedded in Matrigel (356234, Corning, USA) and cultured in DMEM/F12 medium (11320033, Thermo Fisher, USA) supplemented with 100 U/mL penicillin–streptomycin, 10 mM HEPES (15630106, Thermo Fisher), GlutaMAX (35050061, AR TIC LE IN PR ES S Thermo Fisher), B27 (C0347, Beyotime), N2 (17502048, Thermo Fisher), 30% Wnt3a, 10% R-spondin, 10% murine Noggin (conditioned media), 10 μM forskolin (F6886, Sigma), 100 ng/mL murine EGF (SRP3196, Sigma), 100 ng/mL FGF10 (GF172, Sigma), 25 ng/mL HGF (SRP6014, Sigma), 10 nM gastrin I (HY-P1097, MCE), 1.25 mM N-acetylcysteine (50303ES05, Yeasen, China), 10 mM nicotinamide (72340, Sigma), 5 μM A8301 (53002ES03, Yeasen), and 10 μM Y27632 (HY-10071, MCE). ..

    Article Title: Colon-Targeted astragalus polysaccharide nanoparticles prevent NAFLD-Driven hepatocarcinogenesis via microbiota remodeling and NF-κB Inhibition
    Article Snippet: .. The resulting suspension was embedded in Matrigel (356234, Corning, USA) and cultured in DMEM/F12 medium (11320033, Thermo Fisher, USA) supplemented with 100 U/mL penicillin–streptomycin, 10 mM HEPES (15630106, Thermo Fisher), GlutaMAX (35050061, Thermo Fisher), B27 (C0347, Beyotime), N2 (17502048, Thermo Fisher), 30% Wnt3a, 10% R-spondin, 10% murine Noggin (conditioned media), 10 μM forskolin (F6886, Sigma), 100 ng/mL murine EGF (SRP3196, Sigma), 100 ng/mL FGF10 (GF172, Sigma), 25 ng/mL HGF (SRP6014, Sigma), 10 nM gastrin I (HY-P1097, MCE), 1.25 mM N-acetylcysteine (50303ES05, Yeasen, China), 10 mM nicotinamide (72340, Sigma), 5 μM A8301 (53002ES03, Yeasen), and 10 μM Y27632 (HY-10071, MCE). ..

    Control:

    Article Title: G protein-coupled receptor kinase 2 as a novel therapeutic target for gland fibrosis of Sjögren's syndrome.
    Article Snippet: Sjogren’s syndrome (SS) is a chronic, progressive autoimmune disorder characterized by gland fibrosis.. We previously found a close correlation between gland fibrosis and the expression of G protein-coupled receptor kinase 2 (GRK2).. In this study we explored the pathological and therapeutic significance of GRK2 in SS.

    Recombinant:

    Article Title: Tumor-derived colorectal cancer organoids induce a unique Treg cell population by directing CD4 + T cell differentiation
    Article Snippet: The basal BM2 medium for murine and human CRC tumor-organoids culture consisted of Advanced Dulbecco’s Modified Eagle Medium (DMEM)/F12 medium (Gibco) supplemented with 10 mM N’-2-Hydroxyethylpiperazine-N’-2 ethanesulphonic acid (HEPES, Lonza), 2 mM Glutamax (Gibco), 50 U/ml penicillin-streptomycin (Gibco), 100 ng/ml Noggin conditioned medium (produced by lentiviral transfection), 1 mM N-acetylcysteine (Sigma) and 2% B27 serum free supplement (Gibco). .. Then, BM2 mouse (BM2m) medium was further enriched with 10 nM murine recombinant fibroblast growing factor (PeproTech) while BM2 human (BM2h) medium included 500 nM A8301 (MedChemExpress) and 10 μM SB202190 (ApexBio). .. To establish the co-cultures ( A), first, two days old CRC tumor-organoids were retrieved from the BME droplets by adding dispase II (1 mg/ml, Gibco) directly to the medium, followed by a 10-minute incubate at 37 o C with 5% CO 2 .

    Modification:

    Article Title: Omega-3 fatty acid DHA induces ferroptosis in colorectal cancer patient-derived organoids and drug-tolerant cells.
    Article Snippet: .. Complete medium composition was the following: basal medium (Advanced Dulbecco’s modified Eagle medium/F12, penicillin-streptomycin, 2 mM Lglutamine, 1 mM n-Acetyl Cysteine, 10 mM HEPES, B27), supplemented with 50% Wnt3a CM, 10% RspoI-CM, EGF 50 ng/ml, Noggin (Peprotech) 100 ng/ml, A8301 (MedChemExpress) 500 AR TI CL E IN P RE SS nM, Gastrin (Merck) 10 nM, Nicotinammide (Sigma-Aldrich) 5 mM, Primocin (InvivoGen), IGF1 (Peprotech) 100 ng/ml, FGF2 (Peprotech) 50 ng/ml. .. Complete medium composition was the following: basal medium (Advanced Dulbecco’s modified Eagle medium/F12, penicillin-streptomycin, 2 mM Lglutamine, 1 mM n-Acetyl Cysteine, 10 mM HEPES, B27), supplemented with 50% Wnt3a CM, 10% RspoI-CM, EGF 50 ng/ml, Noggin (Peprotech) 100 ng/ml, A8301 (MedChemExpress) 500 AR TI CL E IN P RE SS nM, Gastrin (Merck) 10 nM, Nicotinammide (Sigma-Aldrich) 5 mM, Primocin (InvivoGen), IGF1 (Peprotech) 100 ng/ml, FGF2 (Peprotech) 50 ng/ml.

    Article Title: Omega-3 fatty acid DHA induces ferroptosis in colorectal cancer patient-derived organoids and drug-tolerant cells.
    Article Snippet: Intestinal crypts were centrifuged at 300g for 3 min, washed with cold chelation buffer and centrifuged again at 200g for 3 min. Intestinal crypts were then seeded in 24-wells plate with complete medium plus 10 μM Y27632 (MedChemExpress) and 3 μM CHIR99021 (MedChemExpress) for the first 5-6 days. .. Complete medium composition was the following: basal medium (Advanced Dulbecco’s modified Eagle medium/F12, penicillin-streptomycin, 2 mM Lglutamine, 1 mM n-Acetyl Cysteine, 10 mM HEPES, B27), supplemented with 50% Wnt3a CM, 10% RspoI-CM, EGF 50 ng/ml, Noggin (Peprotech) 100 ng/ml, A8301 (MedChemExpress) 500 AR TI CL E IN P RE SS ARTICLE IN PRESS nM, Gastrin (Merck) 10 nM, Nicotinammide (Sigma-Aldrich) 5 mM, Primocin (InvivoGen), IGF1 (Peprotech) 100 ng/ml, FGF2 (Peprotech) 50 ng/ml. .. Drugs, enzymatic inhibitors and stock solutions of fatty acids Erastin, Ferrostatin-1, RSL-3, Liproxstatin-1, ABT-263 and Oxaliplatin were from MedChemExpress.



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    a8301  (Selleck Chemicals)
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    RBC-MBs promotes the polarization of macrophages towards the M2 phenotype by inhibiting the TLR9-MyD88 and cGAS-STING pathways (A-F) RBC-MB treatment significantly attenuated CpG DNA-induced upregulation of pro-inflammatory cytokines (A-B), key proteins in the TLR9-MyD88 (C-D) and cGAS-STING (E-F) pathways. RAW264.7 cells were treated with 1 μg mL -1 CpG DNA for 24 h, followed by incubation with 0.5 mL RBC-MBs (concentration: 1 × 10 8 particles mL -1 ) for 24 h, and then subjected to western blot analysis (A, C, E). IL-1α, TNF-α, IL-6, TLR9, MyD88, STING, and cGAS levels were normalized to the β-actin level in each sample. p-NF-κB, p-TBK1, and p-IRF3 levels were normalized to their respective total protein levels (NF-κB, TBK1, and IRF3) in each sample. These normalized values were then used for statistical analysis (B, D, F), n = 6. (G) RBC-MB treatment significantly attenuated CpG DNA-induced upregulation of CD86 in macrophages. RAW264.7 cells were treated with 1 μg mL -1 CpG DNA for 24 h, followed by incubation with 0.5 mL RBC-MBs (concentration: 1 × 10 8 particles mL -1 ) for 24 h, then immunostained with an anti-CD86 antibody (green) and DAPI (blue, nuclei), and imaged by fluorescence microscopy. Representative images (left panel) and quantitative fluorescence graph (right panel) are shown. Scale bar: 50 μm, n = 6. (H-J) RBC-MB treatment significantly inhibited hepatic IRI-induced polarization of macrophages toward the M1 phenotype (H) and activation of the TLR9-MyD88 and cGAS-STING pathways (I-J). MASLD rats underwent 45 min of hepatic ischemia followed by reperfusion. At 0, 6, 12, 18, and 24 h post-reperfusion, the rats were treated with either Con-MBs or RBC-MBs. Primary macrophages were isolated from rat livers, then co-stained with CD68 and CD163 followed by flow cytometric analysis (H), n = 3/4, or subjected to western blotting (I-J). CD206, iNOS, TLR9, MyD88, STING, and cGAS levels were normalized to the β-actin level in each sample, and these normalized values were used for statistical analysis (J), n = 3. For (B, D, F, G right panel , H right panel , J), error bars represent mean ± standard deviation. P values were calculated using the unpaired Student's t-test (B, D, F, G right panel ) or one-way analysis of variance (ANOVA) (H right panel , J). *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, not significant.(For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Materials Today Bio

    Article Title: Toll-like receptor 9-overexpressing red blood cell biomimetic microbubbles adsorb cell-free DNA to relieve steatotic liver ischemia-reperfusion injury

    doi: 10.1016/j.mtbio.2026.103558

    Figure Lengend Snippet: RBC-MBs promotes the polarization of macrophages towards the M2 phenotype by inhibiting the TLR9-MyD88 and cGAS-STING pathways (A-F) RBC-MB treatment significantly attenuated CpG DNA-induced upregulation of pro-inflammatory cytokines (A-B), key proteins in the TLR9-MyD88 (C-D) and cGAS-STING (E-F) pathways. RAW264.7 cells were treated with 1 μg mL -1 CpG DNA for 24 h, followed by incubation with 0.5 mL RBC-MBs (concentration: 1 × 10 8 particles mL -1 ) for 24 h, and then subjected to western blot analysis (A, C, E). IL-1α, TNF-α, IL-6, TLR9, MyD88, STING, and cGAS levels were normalized to the β-actin level in each sample. p-NF-κB, p-TBK1, and p-IRF3 levels were normalized to their respective total protein levels (NF-κB, TBK1, and IRF3) in each sample. These normalized values were then used for statistical analysis (B, D, F), n = 6. (G) RBC-MB treatment significantly attenuated CpG DNA-induced upregulation of CD86 in macrophages. RAW264.7 cells were treated with 1 μg mL -1 CpG DNA for 24 h, followed by incubation with 0.5 mL RBC-MBs (concentration: 1 × 10 8 particles mL -1 ) for 24 h, then immunostained with an anti-CD86 antibody (green) and DAPI (blue, nuclei), and imaged by fluorescence microscopy. Representative images (left panel) and quantitative fluorescence graph (right panel) are shown. Scale bar: 50 μm, n = 6. (H-J) RBC-MB treatment significantly inhibited hepatic IRI-induced polarization of macrophages toward the M1 phenotype (H) and activation of the TLR9-MyD88 and cGAS-STING pathways (I-J). MASLD rats underwent 45 min of hepatic ischemia followed by reperfusion. At 0, 6, 12, 18, and 24 h post-reperfusion, the rats were treated with either Con-MBs or RBC-MBs. Primary macrophages were isolated from rat livers, then co-stained with CD68 and CD163 followed by flow cytometric analysis (H), n = 3/4, or subjected to western blotting (I-J). CD206, iNOS, TLR9, MyD88, STING, and cGAS levels were normalized to the β-actin level in each sample, and these normalized values were used for statistical analysis (J), n = 3. For (B, D, F, G right panel , H right panel , J), error bars represent mean ± standard deviation. P values were calculated using the unpaired Student's t-test (B, D, F, G right panel ) or one-way analysis of variance (ANOVA) (H right panel , J). *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, not significant.(For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Primary antibodies used included: rabbit polyclonal antibodies against TLR9 (A14642, Abclonal), NF-κB (A2547, Abclonal), MyD88 (A0980, Abclonal), cGAS (A8335, Abclonal), IL-1α (A2170, Abclonal), TNF-α (A0277, Abclonal), IL-6 (A0286, Abclonal), CD206 (A8301, Abclonal), CD86 (A16805, Abclonal), CD47 (A1838, Abclonal), and ATP1A (A0643, Abclonal); rabbit monoclonal antibodies against STING (13647, CST, Beverly, MA, USA), p-NF-κB (3033, CST), p-TBK1 (5483, CST), TBK1 (ab40676, Abcam), p-IκB (2859, CST), IκB (4812, CST), p-IRF3 (4947, CST), and β-actin (AC026, Abclonal).

    Techniques: Incubation, Concentration Assay, Western Blot, Fluorescence, Microscopy, Activation Assay, Isolation, Staining, Standard Deviation

    Inhibition of NNMT in mPSCs of CP mice limits M2 polarization of mouse macrophages. ( A ) Isolation and culture of bone marrow-derived macrophages (BMDMs) in lentiviral-infected conditional medium. ( B ) The proportion of CD206 + cells was detected by flow cytometry ( n = 3). ( C ) The mRNA levels of Arg1, MRC1, and TGFB1 in the BMDMs ( n = 3). ( D ) Isolation and cultivation of BMDMs in conditional medium with NNMTi. ( E ) The proportion of CD206 + cells was detected by flow cytometry ( n = 3). ( F ) The mRNA levels of Arg1, MRC1, and TGFB1 in the BMDMs ( n = 3).

    Journal: Inflammation

    Article Title: Nicotinamide N-Methyltransferase Inhibition Mitigates Cerulein-Induced Pancreatic Fibrosis via Galectin-3-Mediated Regulation of Stellate Cell Activation and Macrophage M2 Polarization in Mice

    doi: 10.1007/s10753-026-02491-w

    Figure Lengend Snippet: Inhibition of NNMT in mPSCs of CP mice limits M2 polarization of mouse macrophages. ( A ) Isolation and culture of bone marrow-derived macrophages (BMDMs) in lentiviral-infected conditional medium. ( B ) The proportion of CD206 + cells was detected by flow cytometry ( n = 3). ( C ) The mRNA levels of Arg1, MRC1, and TGFB1 in the BMDMs ( n = 3). ( D ) Isolation and cultivation of BMDMs in conditional medium with NNMTi. ( E ) The proportion of CD206 + cells was detected by flow cytometry ( n = 3). ( F ) The mRNA levels of Arg1, MRC1, and TGFB1 in the BMDMs ( n = 3).

    Article Snippet: The primary antibodies are as follow: NNMT antibody (1:3000, 15123-1-AP, Proteintech), α-SMA antibody (1:20000, 67735-1-Ig, Proteintech), H3K27me3 antibody (1:500, DF6941, Affinity, Liyang, Jiangsu, China), Histone H3 antibody (1:1000, AF0863, Affinity), LGALS3 antibody (1:2000, A11198, Abclonal), Arg1 antibody (1:1000, A1847, Abclonal), MRC1 antibody (1:5000, A21014, Abclonal), GAPDH antibody (1:10000, AF7021, Affinity).

    Techniques: Inhibition, Isolation, Derivative Assay, Infection, Flow Cytometry

    Suppression of NNMT impedes macrophage M2 polarization in mice suffering from CP. ( A ) The mRNA levels of Arg1, MRC1, and TGFB1 in mouse pancreatic tissues ( n = 6). ( B ) Immunofluorescence double staining of CD206 and F4/80 in mouse pancreatic tissues ( n = 6). Zoom scale bar = 20 μm, other scale bar = 50 μm. ( C ) Flow cytometry was used to detect the F4/80 + CD206 + cells in mouse pancreatic tissues ( n = 6).

    Journal: Inflammation

    Article Title: Nicotinamide N-Methyltransferase Inhibition Mitigates Cerulein-Induced Pancreatic Fibrosis via Galectin-3-Mediated Regulation of Stellate Cell Activation and Macrophage M2 Polarization in Mice

    doi: 10.1007/s10753-026-02491-w

    Figure Lengend Snippet: Suppression of NNMT impedes macrophage M2 polarization in mice suffering from CP. ( A ) The mRNA levels of Arg1, MRC1, and TGFB1 in mouse pancreatic tissues ( n = 6). ( B ) Immunofluorescence double staining of CD206 and F4/80 in mouse pancreatic tissues ( n = 6). Zoom scale bar = 20 μm, other scale bar = 50 μm. ( C ) Flow cytometry was used to detect the F4/80 + CD206 + cells in mouse pancreatic tissues ( n = 6).

    Article Snippet: The primary antibodies are as follow: NNMT antibody (1:3000, 15123-1-AP, Proteintech), α-SMA antibody (1:20000, 67735-1-Ig, Proteintech), H3K27me3 antibody (1:500, DF6941, Affinity, Liyang, Jiangsu, China), Histone H3 antibody (1:1000, AF0863, Affinity), LGALS3 antibody (1:2000, A11198, Abclonal), Arg1 antibody (1:1000, A1847, Abclonal), MRC1 antibody (1:5000, A21014, Abclonal), GAPDH antibody (1:10000, AF7021, Affinity).

    Techniques: Immunofluorescence, Double Staining, Flow Cytometry

    NNMT regulates M2 polarization of mouse macrophages via LGALS3. ( A ) Isolation and lentivirus infection in mPSCs of CP mice. ( B ) ELISA assay for the expression of LGALS3 in the supernatant of CP-mPSCs ( n = 3). C - D . The mRNA and protein levels of LGALS3 in the mPSCs with LGALS3 overexpression ( n = 3). E . Isolation and culture of BMDMs in lentiviral-infected conditional medium. F - G . The mRNA and protein levels of Arg1 and MRC1 in the BMDMs ( n = 3). H . The proportion of CD206 + cells was detected by flow cytometry ( n = 3).

    Journal: Inflammation

    Article Title: Nicotinamide N-Methyltransferase Inhibition Mitigates Cerulein-Induced Pancreatic Fibrosis via Galectin-3-Mediated Regulation of Stellate Cell Activation and Macrophage M2 Polarization in Mice

    doi: 10.1007/s10753-026-02491-w

    Figure Lengend Snippet: NNMT regulates M2 polarization of mouse macrophages via LGALS3. ( A ) Isolation and lentivirus infection in mPSCs of CP mice. ( B ) ELISA assay for the expression of LGALS3 in the supernatant of CP-mPSCs ( n = 3). C - D . The mRNA and protein levels of LGALS3 in the mPSCs with LGALS3 overexpression ( n = 3). E . Isolation and culture of BMDMs in lentiviral-infected conditional medium. F - G . The mRNA and protein levels of Arg1 and MRC1 in the BMDMs ( n = 3). H . The proportion of CD206 + cells was detected by flow cytometry ( n = 3).

    Article Snippet: The primary antibodies are as follow: NNMT antibody (1:3000, 15123-1-AP, Proteintech), α-SMA antibody (1:20000, 67735-1-Ig, Proteintech), H3K27me3 antibody (1:500, DF6941, Affinity, Liyang, Jiangsu, China), Histone H3 antibody (1:1000, AF0863, Affinity), LGALS3 antibody (1:2000, A11198, Abclonal), Arg1 antibody (1:1000, A1847, Abclonal), MRC1 antibody (1:5000, A21014, Abclonal), GAPDH antibody (1:10000, AF7021, Affinity).

    Techniques: Isolation, Infection, Enzyme-linked Immunosorbent Assay, Expressing, Over Expression, Flow Cytometry