human coronary artery endothelial cells  (ATCC)

Journal: iScience

Article Title: Comparative effects of hemodialysis modalities on microparticle induction and neutrophil activation in a randomized cross-over study

doi: 10.1016/j.isci.2025.113485

Figure Lengend Snippet: Effects of post-hemodialysis microparticles from the mixed dilution online-HDF (OL-HDF) or hemodialysis with median cut-off dialyzer (MCO-HDX) on human coronary artery endothelial cells (HCAECs) The representation of carboxyfluorescein succinimidyl ester (CSFE)-stained MPs (green color) represented by CSFE fluorescent intensity with the representative immunofluorescent pictures (A and B) are demonstrated ( n = 6/group, scale bar = 10 μ m). The red color in A is zona occluden-1 (ZO-1; a tight junction molecule) and the blue color is DNA-stained by 4′,6-diamidino-2-phenylindole (DAPI). The influences of the post-dialysis MPs from OL-HDF (C) or MCO+HDX (D) with 1x10 4 (light color) or 1x10 5 (heavy color) particles/μL at 24 h after inoculation, compared with control (only media but not MPs) in the condition with or without phorbol myristate acetate (PMA, the positive control activator) on HCAECs as indicated by vascular cell adhesion molecule 1 (VCAM-1) and cell apoptosis, are shown. The effect of MPs on tight junction molecules ZO-1 is also demonstrated through the intensity score of the red color with representative immunofluorescent (E, F) ( n = 6/group, scale bar = 10 μ m). Notably, the MPs were not stained to avoid the noise from the green-fluorescent color. ∗ p < 0.05 vs. control within group; # p < 0.05 vs. same condition between groups.

Article Snippet: Primary Human Coronary Endothelial Cells (HCAECs), Female , ATCC , PCS-100-020 Lot No. 70001400.

Techniques: Staining, Control, Positive Control

Journal: bioRxiv

Article Title: Chromatin landscape and enhancer-gene interaction differences between three cardiac cell types

doi: 10.1101/2025.09.23.678180

Figure Lengend Snippet:

Article Snippet: Human coronary artery smooth muscle cells (HCASMC) and Human coronary artery endothelial cells (HCAEC) were all commercially bought from Thermo Fisher Scientific, Waltham, MA, U.S.A. (product code: C-017-5C) and the American Type Culture Collection (ATCC), Manassas, VA, U.S.A. (Product code: PCS-100-020), respectively.

Techniques: Modification

Journal: bioRxiv

Article Title: Mitochondrial Dysfunction in Endothelial Cells Drives Greater Vascular Impairment in Females with Diabetes-Associated Peripheral Artery Disease

doi: 10.1101/2025.09.22.677648

Figure Lengend Snippet: (A) Schematic illustrating site of tissue collection from below- and above knee amputations. (B) Myography showing increased endothelial dysfunction in female vs male patient arteries. Left , endothelial-dependent relaxation in response to acetylcholine (ACh). Right , No difference in vascular smooth muscle cell (VSMC), sodium nitroprusside (SNP)- mediated relaxation (n=3-5). (C) Left, representative image of microvessels in tibialis anterior (CD31 + SMA + , yellow arrows). Laminin (myocytes, white), CD31 (ECs, red) and SMA (pericytes, green); scale bar 50 μm. Right , quantification (n=5/sex). (D) Myocyte area is unaltered with sex (n=5). (E) NADPH oxidase ( Nox ) mRNA marker expression in muscle measured by qPCR, normalized to β -actin (n=5). (F) 8-isoprostane levels in plasma remain unchanged with sex (n=5). Results are mean±SEM; two-way ANOVA, Student’s t -test or Mann–Whitney U -test; * P <0.05, ** P <0.01, *** P <0.001.

Article Snippet: Primary human male and female coronary artery ECs were purchased from American Type Culture Collection.

Techniques: Marker, Expressing, Clinical Proteomics, MANN-WHITNEY

Journal: bioRxiv

Article Title: Mitochondrial Dysfunction in Endothelial Cells Drives Greater Vascular Impairment in Females with Diabetes-Associated Peripheral Artery Disease

doi: 10.1101/2025.09.22.677648

Figure Lengend Snippet: (A) Schematic to show vessel collection for myography. (B) Myography showing increased endothelial dysfunction in female vs male arteries. Left , endothelial-dependent relaxation in response to acetylcholine (ACh). Right , sodium nitroprusside (SNP)-mediated relaxation (n=5-7). (C) Myography in non-injured vessels (N=5-7). (D) Plasma nitrite/nitrate levels (n=4-6). Laser Doppler imaging showing reduced blood perfusion over time with diabetes in (E) male and (F) female mice. Left, representative image of blood flow at 14 d. Right, quantification. (n=9-11). (G) Laser doppler perfusion index at 14 days (n=9-11). (H) Microvessel number (CD31+SMA+, <50 µm in diameter) in gastrocnemius tissues normalized to the number of myocytes and control non-ischemic limbs (n=9-11). (I) Tubule formation of male and female murine ECs shows an altered phenotype. (J) Tubulogenesis is reduced in female ECs under diabetic conditions. Left, Wimasis platform for vessel coverage and networks. Right , quantification (n=5/group). Results are mean±SEM; two-way ANOVA, Student’s t -test or Mann–Whitney U -test; * P <0.05, ** P <0.01, *** P <0.001 and **** P <0.0001.

Article Snippet: Primary human male and female coronary artery ECs were purchased from American Type Culture Collection.

Techniques: Clinical Proteomics, Imaging, Control, MANN-WHITNEY