sporothrix brasiliensis isolates (ATCC)
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sporothrix brasiliensis isolates
Sporothrix Brasiliensis Isolates, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sporothrix brasiliensis isolates/product/ATCC
Average 95 stars, based on 66 article reviews
Sporothrix Brasiliensis Isolates, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sporothrix brasiliensis isolates/product/ATCC
Average 95 stars, based on 66 article reviews
sporothrix brasiliensis isolates - by Bioz Stars,
2026-02
95/100 stars
Images
1) Product Images from "Δ 24 -Sterol Methyltransferase Plays an Important Role in the Growth and Development of Sporothrix schenckii and Sporothrix brasiliensis"
Article Title: Δ 24 -Sterol Methyltransferase Plays an Important Role in the Growth and Development of Sporothrix schenckii and Sporothrix brasiliensis
Journal: Frontiers in Microbiology
doi: 10.3389/fmicb.2016.00311
Figure Legend Snippet: Antifungal activity of 24-SMT inhibitor H3 and itraconazole against Sporothrix schenckii and Sporothrix brasiliensis.
Techniques Used: Activity Assay
Figure Legend Snippet: Time-kill plots showing activity of the 24-SMT inhibitor H3 in comparison with that of itraconazole against the yeast forms of Sporothrix schenckii ATCC MYA 4821 and S. brasiliensis ATCC MYA 4823 .
Techniques Used: Activity Assay, Comparison
Figure Legend Snippet: Free sterols present in Sporothrix schenckii ATCC MYA 4821 and Sporothrix brasilensis ATCC MYA 4823 growth in untreated (control) and treated with H3 o itraconazole.
Techniques Used: Control
Figure Legend Snippet: Ultrastructural analysis of Sporothrix schenckii ATCC MYA 4821 yeast cells treated with 0.125 mg/L H3 (D–F) or 0.25 mg/L itraconazole (G–I) (MIC/2) compared to untreated cells (A–C) . Scanning electron microscopy (A,D,G) and transmission electron microscopy (B,C,E,F,H,I) images show the presence of: m, mitochondria (B) ; n, nucleus (B) ; G, Golgi complex (H) ; inner cell wall (ICW); microfibrillar cell wall layer (ML). The arrowhead in (I) indicates a groove in the cell wall structure. (J) Cell diameter analysis by calculation of Feret diameters. (K) Analysis of ICW and ML thickness as measured in TEM images. ∗∗ p < 0.001; ∗∗∗ p < 0.0001. Scale bars: 5 μm (A,D,G) ; 0.5 μm (B,E,H) ; 0.2 μm (C,F,I) .
Techniques Used: Electron Microscopy, Transmission Assay
Figure Legend Snippet: Ultrastructural analysis of Sporothrix brasiliensis ATCC MYA 4823 cells after exposure to 0.03 mg/L H3 (D–F) or 0.25 mg/L itraconazole (G–I) compared to untreated cells (A–C) . Scanning electron microscopy (A,D,G) and transmission electron microscopy (B,C,E,F,H,I) images show the presence of: m, mitochondria (B) ; n, nucleus (B,H) ; v, vacuole (B) ; inner cell wall (ICW); microfibrillar cell wall layer (ML). Arrowheads indicate small vesicles associated with the plasma membrane (I) . (J) Cell diameter analysis by calculation of Feret diameters. (K) Analysis of ICW and ML thickness as measured in TEM images. ∗ p < 0.05, ∗∗ p < 0.001, ∗∗∗ p < 0.0001. Scale bars: 5 μm (A,D,G) ; 0.5 μm (B,E,H) ; 0.2 μm (C,F,I) .
Techniques Used: Electron Microscopy, Transmission Assay, Clinical Proteomics, Membrane
Figure Legend Snippet: Mitochondrial activity after treatment of Sporothrix schenckii ATCC MYA 4821 and S. brasiliensis ATCC MYA 4823 yeast cells with H3 or itraconazole. Cells untreated or treated for 96 h with sub-inhibitory concentrations of H3 or itraconazole were stained with MitoTracker Red CMXRos, after which fluorescence intensity was analyzed by flow cytometry. H3 exposure induced a statistically significant fluorescence intensity increase ( S. schenckii 4821) or decrease ( S. brasiliensis 4823), reflecting mitochondrial disturbance ( ∗ p < 0.05).
Techniques Used: Activity Assay, Staining, Fluorescence, Flow Cytometry
Figure Legend Snippet: Associations of itraconazole and H3 against Sporothrix schenckii and Sporothrix brasiliensis yeast cells. Minimum inhibitory concentration (MIC) values are in mg/L.
Techniques Used: Concentration Assay
Figure Legend Snippet: Selectivity of 24-SMT inhibitor H3, compared to itraconazole, toward Sporothrix schenckii and Sporothrix brasiliensis yeast cells.
Techniques Used: Activity Assay
Figure Legend Snippet: Representative pathway of sterol biosynthesis in Sporothrix schenckii and Sporothrix brasiliensis , and sites of action of the studied inhibitors. Sterols identified on Table are shown in this figure: (1) 14α-methyl-cholesta-8-en-3-one, (2) ergosterol, (3) 14α-methyl-ergosta-8,24(24’)-dien-3β-ol, (4) ergosta-5,7,22,24-tetra-en-3β-ol, (5) ergosta-5,7,24(24′)-trien-3β-ol, (6) 5-dehydro-episterol, (7) 4,14-dimethyl-ergosta-5,7,24(24’)-trien-3β-ol, (8) stigmasterol, (9) obtusifoliol, (10) lanosta-8,24-dien-3-one, (11) lanosterol, (12) 24-methylene-lanosta-8-en-3-one, (13) eburicol, (14) 24-ethyl-lanosta-8,22-dien-3β-ol, (15) 24( E )-ethylidenelanost-8-en-3β -ol, (16) 24( Z )-ethylidenelanost-8-en-3β-ol, (17) 4,4-dimethyl-ergosta-8,14,24(24′)-trien-3β-ol, (18) 4,4′-dimethyl-ergosta-8,24(24′)-dien-3β-ol, (19) 4-methyl-ergosta-8,24(24′)-dien-3β-ol, (20) fecosterol, (21) 4,4-dimethyl-ergosta-8,14,24-trien-3β-ol, (22) 4,4-dimethyl-ergosta-8,24-dien-3β-ol, (23) 4-methyl-ergosta-8,24-dien-3β-ol, and (24) zymosterol. Thick lines indicate the main pathways (pathway A or B) from lanosterol to ergosterol. Dashed arrows show accumulation of intermediate sterols by action of the sterol biosynthesis inhibitors itraconazole and H3 (pathways C, D, E). Arrows marked with an ‘X’ indicate inhibitory interactions of the sterol hydrazone H3 and itraconazole with Δ 24 -sterol methyl transferase (24-SMT 1 ) and C14α-demethylase, respectively. 24-SMR, Δ 24 -sterol methyl reductase.
Techniques Used: