Mutagenesis:Article Title: Improvement of Nemadectin Production by Overexpressing the Regulatory Gene nemR and Nemadectin Biosynthetic Gene Cluster in Streptomyces Cyaneogriseus
Article Snippet: .. Table 1 Strains and plasmids used in this study Strains or plasmids Relevant characteristic Reference or source Strains E. coli DH5α F , f80lacZDM15, D(lacZYA-argF) U169, deoR, recA1, endA1, hsdR17 (rk , mkþ), phoA, supE44, λ , thi-1, gyrA96, relA1 CWBIO S17–1 supE44, DlacU169 (FlacZDM15), recA1, endA1, hsdR17, thi-1, gyrA96, relA1, par phage lysogenic Gibco-BRL EPI300 F mcrAD(mrr-hsdRMS-mcrBC)Φ80dlacZDM15DlacX74 recA1 endA1 araD139 D (ara, leu)7697 galU galK λ- rpsL (StrR) nupG trfA tonA Epicentre S. cerevisiae VL6–48 MATα his3-D200 trp1-D1 ura3–52 ys2ade2–101 met14 psiþ cir0 ATCC MYA-366 S. cyaneogriseus MOX-101 Nemadectin producer This laboratory DnemR MOX-101 with deleted nemR This study DnemR/pnemR DnemR mutant with pnemR This study MOX-101/pSET152 MOX-101 strain with pSET152 This study MOX-101/pnemR MOX-101 strain with pnemR This study MOX-101/pnemR-ermEp MOX-101 strain with pnemR-ermEp This study MOX-101/pCL01 MOX-101 strain with pCL01 This study MOX-101/pCL-MOX MOX-101 strain with pCL-MOX This study Plasmids pSET152 E. coli replicon, Streptomyces ØC31 attachment site, AprR This laboratory pKCCas9(tipAp) pKC1139 with the scocas9 gene under the control of the inducible promoter tipAp 16 pCL01 Large gene cluster capture vector: derived from pCC1BAC containing a single copy F-factor replicon and a high-copy origin of replication,ΦC31 int/attP, aac(3)IV, oriT RK2, ARSH4/CEN6, and TRP1 17 pDnemR pSET152 with the upstream and downstream DNA fragments of nemR This study pnemR pSET152 with the expression of nemR under the control of native promoter This study pnemR-ermEp pSET152 with the expression of nemR under the control of strong promoter ermEp This study pCL-M1 pCL01 with the upstream and downstream DNA fragments of the gene cluster MOX1 This study pCL-M2 pCL01 with the upstream and downstream DNA fragments of the gene cluster MOX2 This study pCL-M pCL01 with the upstream and downstream DNA fragments of the gene cluster MOX This study pCL-MOX1 pCL-M1 with the biosynthetic gene cluster MOX1 This study pCL-MOX2 pCL-M2 with the biosynthetic gene cluster MOX2 This study pCL-MOX pCL-M with the biosynthetic gene cluster MOX This study Pharmaceutical Fronts Vol. ..
Control:Article Title: Improvement of Nemadectin Production by Overexpressing the Regulatory Gene nemR and Nemadectin Biosynthetic Gene Cluster in Streptomyces Cyaneogriseus
Article Snippet: .. Table 1 Strains and plasmids used in this study Strains or plasmids Relevant characteristic Reference or source Strains E. coli DH5α F , f80lacZDM15, D(lacZYA-argF) U169, deoR, recA1, endA1, hsdR17 (rk , mkþ), phoA, supE44, λ , thi-1, gyrA96, relA1 CWBIO S17–1 supE44, DlacU169 (FlacZDM15), recA1, endA1, hsdR17, thi-1, gyrA96, relA1, par phage lysogenic Gibco-BRL EPI300 F mcrAD(mrr-hsdRMS-mcrBC)Φ80dlacZDM15DlacX74 recA1 endA1 araD139 D (ara, leu)7697 galU galK λ- rpsL (StrR) nupG trfA tonA Epicentre S. cerevisiae VL6–48 MATα his3-D200 trp1-D1 ura3–52 ys2ade2–101 met14 psiþ cir0 ATCC MYA-366 S. cyaneogriseus MOX-101 Nemadectin producer This laboratory DnemR MOX-101 with deleted nemR This study DnemR/pnemR DnemR mutant with pnemR This study MOX-101/pSET152 MOX-101 strain with pSET152 This study MOX-101/pnemR MOX-101 strain with pnemR This study MOX-101/pnemR-ermEp MOX-101 strain with pnemR-ermEp This study MOX-101/pCL01 MOX-101 strain with pCL01 This study MOX-101/pCL-MOX MOX-101 strain with pCL-MOX This study Plasmids pSET152 E. coli replicon, Streptomyces ØC31 attachment site, AprR This laboratory pKCCas9(tipAp) pKC1139 with the scocas9 gene under the control of the inducible promoter tipAp 16 pCL01 Large gene cluster capture vector: derived from pCC1BAC containing a single copy F-factor replicon and a high-copy origin of replication,ΦC31 int/attP, aac(3)IV, oriT RK2, ARSH4/CEN6, and TRP1 17 pDnemR pSET152 with the upstream and downstream DNA fragments of nemR This study pnemR pSET152 with the expression of nemR under the control of native promoter This study pnemR-ermEp pSET152 with the expression of nemR under the control of strong promoter ermEp This study pCL-M1 pCL01 with the upstream and downstream DNA fragments of the gene cluster MOX1 This study pCL-M2 pCL01 with the upstream and downstream DNA fragments of the gene cluster MOX2 This study pCL-M pCL01 with the upstream and downstream DNA fragments of the gene cluster MOX This study pCL-MOX1 pCL-M1 with the biosynthetic gene cluster MOX1 This study pCL-MOX2 pCL-M2 with the biosynthetic gene cluster MOX2 This study pCL-MOX pCL-M with the biosynthetic gene cluster MOX This study Pharmaceutical Fronts Vol. ..
Derivative Assay:Article Title: Improvement of Nemadectin Production by Overexpressing the Regulatory Gene nemR and Nemadectin Biosynthetic Gene Cluster in Streptomyces Cyaneogriseus
Article Snippet: .. Table 1 Strains and plasmids used in this study Strains or plasmids Relevant characteristic Reference or source Strains E. coli DH5α F , f80lacZDM15, D(lacZYA-argF) U169, deoR, recA1, endA1, hsdR17 (rk , mkþ), phoA, supE44, λ , thi-1, gyrA96, relA1 CWBIO S17–1 supE44, DlacU169 (FlacZDM15), recA1, endA1, hsdR17, thi-1, gyrA96, relA1, par phage lysogenic Gibco-BRL EPI300 F mcrAD(mrr-hsdRMS-mcrBC)Φ80dlacZDM15DlacX74 recA1 endA1 araD139 D (ara, leu)7697 galU galK λ- rpsL (StrR) nupG trfA tonA Epicentre S. cerevisiae VL6–48 MATα his3-D200 trp1-D1 ura3–52 ys2ade2–101 met14 psiþ cir0 ATCC MYA-366 S. cyaneogriseus MOX-101 Nemadectin producer This laboratory DnemR MOX-101 with deleted nemR This study DnemR/pnemR DnemR mutant with pnemR This study MOX-101/pSET152 MOX-101 strain with pSET152 This study MOX-101/pnemR MOX-101 strain with pnemR This study MOX-101/pnemR-ermEp MOX-101 strain with pnemR-ermEp This study MOX-101/pCL01 MOX-101 strain with pCL01 This study MOX-101/pCL-MOX MOX-101 strain with pCL-MOX This study Plasmids pSET152 E. coli replicon, Streptomyces ØC31 attachment site, AprR This laboratory pKCCas9(tipAp) pKC1139 with the scocas9 gene under the control of the inducible promoter tipAp 16 pCL01 Large gene cluster capture vector: derived from pCC1BAC containing a single copy F-factor replicon and a high-copy origin of replication,ΦC31 int/attP, aac(3)IV, oriT RK2, ARSH4/CEN6, and TRP1 17 pDnemR pSET152 with the upstream and downstream DNA fragments of nemR This study pnemR pSET152 with the expression of nemR under the control of native promoter This study pnemR-ermEp pSET152 with the expression of nemR under the control of strong promoter ermEp This study pCL-M1 pCL01 with the upstream and downstream DNA fragments of the gene cluster MOX1 This study pCL-M2 pCL01 with the upstream and downstream DNA fragments of the gene cluster MOX2 This study pCL-M pCL01 with the upstream and downstream DNA fragments of the gene cluster MOX This study pCL-MOX1 pCL-M1 with the biosynthetic gene cluster MOX1 This study pCL-MOX2 pCL-M2 with the biosynthetic gene cluster MOX2 This study pCL-MOX pCL-M with the biosynthetic gene cluster MOX This study Pharmaceutical Fronts Vol. ..
Expressing:Article Title: Improvement of Nemadectin Production by Overexpressing the Regulatory Gene nemR and Nemadectin Biosynthetic Gene Cluster in Streptomyces Cyaneogriseus
Article Snippet: .. Table 1 Strains and plasmids used in this study Strains or plasmids Relevant characteristic Reference or source Strains E. coli DH5α F , f80lacZDM15, D(lacZYA-argF) U169, deoR, recA1, endA1, hsdR17 (rk , mkþ), phoA, supE44, λ , thi-1, gyrA96, relA1 CWBIO S17–1 supE44, DlacU169 (FlacZDM15), recA1, endA1, hsdR17, thi-1, gyrA96, relA1, par phage lysogenic Gibco-BRL EPI300 F mcrAD(mrr-hsdRMS-mcrBC)Φ80dlacZDM15DlacX74 recA1 endA1 araD139 D (ara, leu)7697 galU galK λ- rpsL (StrR) nupG trfA tonA Epicentre S. cerevisiae VL6–48 MATα his3-D200 trp1-D1 ura3–52 ys2ade2–101 met14 psiþ cir0 ATCC MYA-366 S. cyaneogriseus MOX-101 Nemadectin producer This laboratory DnemR MOX-101 with deleted nemR This study DnemR/pnemR DnemR mutant with pnemR This study MOX-101/pSET152 MOX-101 strain with pSET152 This study MOX-101/pnemR MOX-101 strain with pnemR This study MOX-101/pnemR-ermEp MOX-101 strain with pnemR-ermEp This study MOX-101/pCL01 MOX-101 strain with pCL01 This study MOX-101/pCL-MOX MOX-101 strain with pCL-MOX This study Plasmids pSET152 E. coli replicon, Streptomyces ØC31 attachment site, AprR This laboratory pKCCas9(tipAp) pKC1139 with the scocas9 gene under the control of the inducible promoter tipAp 16 pCL01 Large gene cluster capture vector: derived from pCC1BAC containing a single copy F-factor replicon and a high-copy origin of replication,ΦC31 int/attP, aac(3)IV, oriT RK2, ARSH4/CEN6, and TRP1 17 pDnemR pSET152 with the upstream and downstream DNA fragments of nemR This study pnemR pSET152 with the expression of nemR under the control of native promoter This study pnemR-ermEp pSET152 with the expression of nemR under the control of strong promoter ermEp This study pCL-M1 pCL01 with the upstream and downstream DNA fragments of the gene cluster MOX1 This study pCL-M2 pCL01 with the upstream and downstream DNA fragments of the gene cluster MOX2 This study pCL-M pCL01 with the upstream and downstream DNA fragments of the gene cluster MOX This study pCL-MOX1 pCL-M1 with the biosynthetic gene cluster MOX1 This study pCL-MOX2 pCL-M2 with the biosynthetic gene cluster MOX2 This study pCL-MOX pCL-M with the biosynthetic gene cluster MOX This study Pharmaceutical Fronts Vol. ..
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