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Dsdna Oligo, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bamhi methyltransferase  (New England Biolabs)
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NT-seq detects both adenine and cytosine methylation in oligonucleotides. a The inverse of A to G ratio at adenine sites between unmodified control and 6mA modified oligo. b Linear regression between A to G frequency at 6mA site and the percentage of 6mA modified oligo. c The inverse of C to T ratio at cytosine sites between unmodified oligo and oligo modified by <t>BamHI</t> <t>methyltransferase.</t> d Linear regression between C to T frequency at 4mC position 39 and the percentage of 4mC modified oligo. e Linear regression between C to T frequency at 4mC position 42 and the percentage of 4mC modified oligo. f C to T ratio at cytosine sites between unmodified control and 5mC modified oligo. Modified adenine or cytosine sites are labeled in red. Adenine or cytosine sites inside the primer regions are not included. Dots represent the mean and error bars represent standard deviation. All samples were replicated three times. One replicate for 25%, 50%, and 100% of 4mC modified oligo samples was not used due to library prep and sequencing depth issues
Bamhi Methyltransferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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m0223s  (New England Biolabs)
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NT-seq detects both adenine and cytosine methylation in oligonucleotides. a The inverse of A to G ratio at adenine sites between unmodified control and 6mA modified oligo. b Linear regression between A to G frequency at 6mA site and the percentage of 6mA modified oligo. c The inverse of C to T ratio at cytosine sites between unmodified oligo and oligo modified by <t>BamHI</t> <t>methyltransferase.</t> d Linear regression between C to T frequency at 4mC position 39 and the percentage of 4mC modified oligo. e Linear regression between C to T frequency at 4mC position 42 and the percentage of 4mC modified oligo. f C to T ratio at cytosine sites between unmodified control and 5mC modified oligo. Modified adenine or cytosine sites are labeled in red. Adenine or cytosine sites inside the primer regions are not included. Dots represent the mean and error bars represent standard deviation. All samples were replicated three times. One replicate for 25%, 50%, and 100% of 4mC modified oligo samples was not used due to library prep and sequencing depth issues
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bam hi dna methyltransferase  (New England Biolabs)
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NT-seq detects both adenine and cytosine methylation in oligonucleotides. a The inverse of A to G ratio at adenine sites between unmodified control and 6mA modified oligo. b Linear regression between A to G frequency at 6mA site and the percentage of 6mA modified oligo. c The inverse of C to T ratio at cytosine sites between unmodified oligo and oligo modified by <t>BamHI</t> <t>methyltransferase.</t> d Linear regression between C to T frequency at 4mC position 39 and the percentage of 4mC modified oligo. e Linear regression between C to T frequency at 4mC position 42 and the percentage of 4mC modified oligo. f C to T ratio at cytosine sites between unmodified control and 5mC modified oligo. Modified adenine or cytosine sites are labeled in red. Adenine or cytosine sites inside the primer regions are not included. Dots represent the mean and error bars represent standard deviation. All samples were replicated three times. One replicate for 25%, 50%, and 100% of 4mC modified oligo samples was not used due to library prep and sequencing depth issues
Bam Hi Dna Methyltransferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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m bamhi methyltransferase reaction buffer  (New England Biolabs)
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NT-seq detects both adenine and cytosine methylation in oligonucleotides. a The inverse of A to G ratio at adenine sites between unmodified control and 6mA modified oligo. b Linear regression between A to G frequency at 6mA site and the percentage of 6mA modified oligo. c The inverse of C to T ratio at cytosine sites between unmodified oligo and oligo modified by <t>BamHI</t> <t>methyltransferase.</t> d Linear regression between C to T frequency at 4mC position 39 and the percentage of 4mC modified oligo. e Linear regression between C to T frequency at 4mC position 42 and the percentage of 4mC modified oligo. f C to T ratio at cytosine sites between unmodified control and 5mC modified oligo. Modified adenine or cytosine sites are labeled in red. Adenine or cytosine sites inside the primer regions are not included. Dots represent the mean and error bars represent standard deviation. All samples were replicated three times. One replicate for 25%, 50%, and 100% of 4mC modified oligo samples was not used due to library prep and sequencing depth issues
M Bamhi Methyltransferase Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bamhi methyltransferase m bamhi  (New England Biolabs)
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NT-seq detects both adenine and cytosine methylation in oligonucleotides. a The inverse of A to G ratio at adenine sites between unmodified control and 6mA modified oligo. b Linear regression between A to G frequency at 6mA site and the percentage of 6mA modified oligo. c The inverse of C to T ratio at cytosine sites between unmodified oligo and oligo modified by <t>BamHI</t> <t>methyltransferase.</t> d Linear regression between C to T frequency at 4mC position 39 and the percentage of 4mC modified oligo. e Linear regression between C to T frequency at 4mC position 42 and the percentage of 4mC modified oligo. f C to T ratio at cytosine sites between unmodified control and 5mC modified oligo. Modified adenine or cytosine sites are labeled in red. Adenine or cytosine sites inside the primer regions are not included. Dots represent the mean and error bars represent standard deviation. All samples were replicated three times. One replicate for 25%, 50%, and 100% of 4mC modified oligo samples was not used due to library prep and sequencing depth issues
Bamhi Methyltransferase M Bamhi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1x m bamhi methyltransferase 1242 reaction buffer  (New England Biolabs)
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New England Biolabs 1x m bamhi methyltransferase 1242 reaction buffer
NT-seq detects both adenine and cytosine methylation in oligonucleotides. a The inverse of A to G ratio at adenine sites between unmodified control and 6mA modified oligo. b Linear regression between A to G frequency at 6mA site and the percentage of 6mA modified oligo. c The inverse of C to T ratio at cytosine sites between unmodified oligo and oligo modified by <t>BamHI</t> <t>methyltransferase.</t> d Linear regression between C to T frequency at 4mC position 39 and the percentage of 4mC modified oligo. e Linear regression between C to T frequency at 4mC position 42 and the percentage of 4mC modified oligo. f C to T ratio at cytosine sites between unmodified control and 5mC modified oligo. Modified adenine or cytosine sites are labeled in red. Adenine or cytosine sites inside the primer regions are not included. Dots represent the mean and error bars represent standard deviation. All samples were replicated three times. One replicate for 25%, 50%, and 100% of 4mC modified oligo samples was not used due to library prep and sequencing depth issues
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https://www.bioz.com/result/1x m bamhi methyltransferase 1242 reaction buffer/product/New England Biolabs
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m bamhi methyltransferase  (New England Biolabs)
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New England Biolabs m bamhi methyltransferase
NT-seq detects both adenine and cytosine methylation in oligonucleotides. a The inverse of A to G ratio at adenine sites between unmodified control and 6mA modified oligo. b Linear regression between A to G frequency at 6mA site and the percentage of 6mA modified oligo. c The inverse of C to T ratio at cytosine sites between unmodified oligo and oligo modified by <t>BamHI</t> <t>methyltransferase.</t> d Linear regression between C to T frequency at 4mC position 39 and the percentage of 4mC modified oligo. e Linear regression between C to T frequency at 4mC position 42 and the percentage of 4mC modified oligo. f C to T ratio at cytosine sites between unmodified control and 5mC modified oligo. Modified adenine or cytosine sites are labeled in red. Adenine or cytosine sites inside the primer regions are not included. Dots represent the mean and error bars represent standard deviation. All samples were replicated three times. One replicate for 25%, 50%, and 100% of 4mC modified oligo samples was not used due to library prep and sequencing depth issues
M Bamhi Methyltransferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m bamhi methyltransferase/product/New England Biolabs
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cutsmart buffer  (New England Biolabs)
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New England Biolabs cutsmart buffer
NT-seq detects both adenine and cytosine methylation in oligonucleotides. a The inverse of A to G ratio at adenine sites between unmodified control and 6mA modified oligo. b Linear regression between A to G frequency at 6mA site and the percentage of 6mA modified oligo. c The inverse of C to T ratio at cytosine sites between unmodified oligo and oligo modified by <t>BamHI</t> <t>methyltransferase.</t> d Linear regression between C to T frequency at 4mC position 39 and the percentage of 4mC modified oligo. e Linear regression between C to T frequency at 4mC position 42 and the percentage of 4mC modified oligo. f C to T ratio at cytosine sites between unmodified control and 5mC modified oligo. Modified adenine or cytosine sites are labeled in red. Adenine or cytosine sites inside the primer regions are not included. Dots represent the mean and error bars represent standard deviation. All samples were replicated three times. One replicate for 25%, 50%, and 100% of 4mC modified oligo samples was not used due to library prep and sequencing depth issues
Cutsmart Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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NT-seq detects both adenine and cytosine methylation in oligonucleotides. a The inverse of A to G ratio at adenine sites between unmodified control and 6mA modified oligo. b Linear regression between A to G frequency at 6mA site and the percentage of 6mA modified oligo. c The inverse of C to T ratio at cytosine sites between unmodified oligo and oligo modified by BamHI methyltransferase. d Linear regression between C to T frequency at 4mC position 39 and the percentage of 4mC modified oligo. e Linear regression between C to T frequency at 4mC position 42 and the percentage of 4mC modified oligo. f C to T ratio at cytosine sites between unmodified control and 5mC modified oligo. Modified adenine or cytosine sites are labeled in red. Adenine or cytosine sites inside the primer regions are not included. Dots represent the mean and error bars represent standard deviation. All samples were replicated three times. One replicate for 25%, 50%, and 100% of 4mC modified oligo samples was not used due to library prep and sequencing depth issues

Journal: Genome Biology

Article Title: NT-seq: a chemical-based sequencing method for genomic methylome profiling

doi: 10.1186/s13059-022-02689-9

Figure Lengend Snippet: NT-seq detects both adenine and cytosine methylation in oligonucleotides. a The inverse of A to G ratio at adenine sites between unmodified control and 6mA modified oligo. b Linear regression between A to G frequency at 6mA site and the percentage of 6mA modified oligo. c The inverse of C to T ratio at cytosine sites between unmodified oligo and oligo modified by BamHI methyltransferase. d Linear regression between C to T frequency at 4mC position 39 and the percentage of 4mC modified oligo. e Linear regression between C to T frequency at 4mC position 42 and the percentage of 4mC modified oligo. f C to T ratio at cytosine sites between unmodified control and 5mC modified oligo. Modified adenine or cytosine sites are labeled in red. Adenine or cytosine sites inside the primer regions are not included. Dots represent the mean and error bars represent standard deviation. All samples were replicated three times. One replicate for 25%, 50%, and 100% of 4mC modified oligo samples was not used due to library prep and sequencing depth issues

Article Snippet: 4mC modified oligo (91bp) was generated by treating dsDNA oligo with BamHI methyltransferase (methylates GGATCC motif at the first cytosine base) according to the manufacturer’s instructions (NEB, M0223S).

Techniques: Methylation, Modification, Labeling, Standard Deviation, Sequencing