Journal: Science Advances
Article Title: A functional proteomics platform to reveal the sequence determinants of lysine methyltransferase substrate selectivity
Figure Lengend Snippet: K-OPL reveals the substrate selectivity of G9a, SET7/9, and SMYD2. K-OPL substrate selectivity profiles for G9a ( A ), SET7/9 ( B ), and SMYD2 ( C ). Mean results of two independent K-OPL SPA screens for each enzyme are reported as position-normalized heat maps (see fig. S1 for global normalized heat maps and raw K-OPL data). The color code is proportional to the creation of enzyme product, where red (1) is most active and blue (0) is least active. Rows show the identity of each fixed residue, and columns show the position within the sequence. Initial rate measurements with peptides corresponding to known and newly identified substrates for G9a ( D ), SET7/9 ( E ), or SMYD2 ( F ). cpm, counts per minute. Point mutations predicted to decrease or increase the rate of methylation are indicated in red or green, respectively. Data points are shown as the mean of three independent measurements, and error is presented as ±SEM. For some data points, error bars are masked by the symbol weight.
Article Snippet: SET7/9 was purchased from New England BioLabs (catalog no. M0223).
Techniques: Sequencing, Methylation