atcc no  (ATCC)

Journal: bioRxiv

Article Title: “Targeting LARP1 Enhances Carboplatin Sensitivity and Suppresses Tumor Growth in Endometrial Cancer”

doi: 10.64898/2026.03.22.713473

Figure Lengend Snippet: (A, B) Kaplan-Meier plots showing that LARP1 overexpression is associated with shorter overall survival (A) and progression-free interval (B) in endometrial cancer cohort (n=454). (C) Immunoblot analysis illustrating the protein expression of LARP1 after transfecting ISHI cell line with control or LARP1 siRNA at 25, 50, 75, and 100 nM. β-actin was used as a loading control. (D) Images derived from immunofluorescence analysis showing the protein expression of LARP1 after transfecting HEC-1A cells with control or LARP1 siRNA at 75 µM. Scale bar = 50 µM.

Article Snippet: Human endometrial cancer cell lines HEC-1A and Ishikawa (ISHI) were obtained from ATCC (American Type Culture Collection, Manassas, VA, USA).

Techniques: Over Expression, Western Blot, Expressing, Control, Derivative Assay, Immunofluorescence

Journal: bioRxiv

Article Title: “Targeting LARP1 Enhances Carboplatin Sensitivity and Suppresses Tumor Growth in Endometrial Cancer”

doi: 10.64898/2026.03.22.713473

Figure Lengend Snippet: (A – D) Cell viability assay showing the effect of two different LARP1 siRNA (1 and 2) on the viability of ISHI (A, B) and HEC-1A (C, D) cell lines. One-way analysis of variance (ANOVA) was used to assess statistical significance among groups, followed by Tukey’s post hoc test for multiple pairwise comparisons. Data are presented as mean ± SD, with individual data points shown as dots. (E) Immunoblot analysis showing the protein expression of cleaved PARP1 and cleaved caspase 3 after transfection of ISHI and HEC-1A cells with control or LARP1 siRNAs. β-actin was used as a loading control.

Article Snippet: Human endometrial cancer cell lines HEC-1A and Ishikawa (ISHI) were obtained from ATCC (American Type Culture Collection, Manassas, VA, USA).

Techniques: Viability Assay, Western Blot, Expressing, Transfection, Control

Journal: bioRxiv

Article Title: “Targeting LARP1 Enhances Carboplatin Sensitivity and Suppresses Tumor Growth in Endometrial Cancer”

doi: 10.64898/2026.03.22.713473

Figure Lengend Snippet: (A, B) Immunoblot analysis showing the protein expression of LARP1 and E2F1 after transfecting ISHI (A) and HEC-1A (B) cells with control or LARP1 siRNA. β-actin was used as a loading control. (C) Images representing immunofluorescence staining of LARP1 (green) and E2F1 (red) in HEC-1A cells after transfection with control or LARP1 siRNA. DAPI was used as a counter stain. Scale bar = 50 µm.

Article Snippet: Human endometrial cancer cell lines HEC-1A and Ishikawa (ISHI) were obtained from ATCC (American Type Culture Collection, Manassas, VA, USA).

Techniques: Western Blot, Expressing, Control, Immunofluorescence, Staining, Transfection

Journal: bioRxiv

Article Title: “Targeting LARP1 Enhances Carboplatin Sensitivity and Suppresses Tumor Growth in Endometrial Cancer”

doi: 10.64898/2026.03.22.713473

Figure Lengend Snippet: (A, B) MTS cell viability assay showing the effect of escalating concentration of carboplatin on the viability of ISHI (A) and HEC-1A cell lines. Sterile water was used as a vehicle control. One-way analysis of variance (ANOVA) was used to assess statistical significance among groups, followed by Tukey’s post hoc test for multiple pairwise comparisons. Data are presented as mean ± SD, with individual data points shown as dots.

Article Snippet: Human endometrial cancer cell lines HEC-1A and Ishikawa (ISHI) were obtained from ATCC (American Type Culture Collection, Manassas, VA, USA).

Techniques: Viability Assay, Concentration Assay, Sterility, Control

Journal: bioRxiv

Article Title: “Targeting LARP1 Enhances Carboplatin Sensitivity and Suppresses Tumor Growth in Endometrial Cancer”

doi: 10.64898/2026.03.22.713473

Figure Lengend Snippet: (A, C) Micrographs representing colony formation assay showing the effect of LARP1 transfection and carboplatin treatment on the clonogenic survival of ISHI (A) and HEC-1A (B) cell lines. (B, D) Quantitative analysis of clonogenic assay showing the effect of LARP1 knockdown and carboplatin treatment on the clonogenic survival of ISHI (B) and HEC-1A (D) cells. One-way analysis of variance (ANOVA) was used to assess statistical significance among groups, followed by Tukey’s post hoc test for multiple pairwise comparisons. Data are presented as mean ± SD, with individual data points shown as dots.

Article Snippet: Human endometrial cancer cell lines HEC-1A and Ishikawa (ISHI) were obtained from ATCC (American Type Culture Collection, Manassas, VA, USA).

Techniques: Colony Assay, Transfection, Clonogenic Assay, Knockdown