Mouse Assay:Article Title: Ptprf is the conserved receptor for Asprosin's glucogenic effects in vertebrates.
Article Snippet: HEK293, HEK293T, HepG2 and Hepa1-6 (ATCC) cells were maintained at 37◦C and 5% CO2 in DMEM (Thermo Fisher), respectively, containing 10% FBS (Gibco) and 100 mg/mL penicillin-streptomycin (Gibco). .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines HEK293 ATCC CRL-1573 HEK293T ATCC CRL-3216 HepG2 ATCC HB-8065 Hepa1-6 ATCC CRL-1830 Experimental models: Organisms/strains Zebrafish: AB strain China Zebrafish Resource Center N/A Mice: C57BL/6NCya-Ptprfem1/Cya cyagen Cat# S-KO-03926 Oligonucleotides Primers, see Table S1 This paper N/A siRNA targeting sequence: PTPRF #1: CCCUUUAAG AUUCUGUACAUU This paper N/A Software and algorithms GraphPad Prism 8.0 Graphpad Software https://www.graphpad.com/ Gator One software Gator Bio https://www.gatorbio.com/software/ 14 Cell Reports 45, 116974, February 24, 2026 .. In zebrafish studies, the C-terminal peptide of zebrafish Fbn1 and the extracellular ligand-binding domains (LBD) of Ptprfa and Ptprfb were expressed through GenScript (China).
Sequencing:Article Title: Ptprf is the conserved receptor for Asprosin's glucogenic effects in vertebrates.
Article Snippet: HEK293, HEK293T, HepG2 and Hepa1-6 (ATCC) cells were maintained at 37◦C and 5% CO2 in DMEM (Thermo Fisher), respectively, containing 10% FBS (Gibco) and 100 mg/mL penicillin-streptomycin (Gibco). .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines HEK293 ATCC CRL-1573 HEK293T ATCC CRL-3216 HepG2 ATCC HB-8065 Hepa1-6 ATCC CRL-1830 Experimental models: Organisms/strains Zebrafish: AB strain China Zebrafish Resource Center N/A Mice: C57BL/6NCya-Ptprfem1/Cya cyagen Cat# S-KO-03926 Oligonucleotides Primers, see Table S1 This paper N/A siRNA targeting sequence: PTPRF #1: CCCUUUAAG AUUCUGUACAUU This paper N/A Software and algorithms GraphPad Prism 8.0 Graphpad Software https://www.graphpad.com/ Gator One software Gator Bio https://www.gatorbio.com/software/ 14 Cell Reports 45, 116974, February 24, 2026 .. In zebrafish studies, the C-terminal peptide of zebrafish Fbn1 and the extracellular ligand-binding domains (LBD) of Ptprfa and Ptprfb were expressed through GenScript (China).
Software:Article Title: Ptprf is the conserved receptor for Asprosin's glucogenic effects in vertebrates.
Article Snippet: HEK293, HEK293T, HepG2 and Hepa1-6 (ATCC) cells were maintained at 37◦C and 5% CO2 in DMEM (Thermo Fisher), respectively, containing 10% FBS (Gibco) and 100 mg/mL penicillin-streptomycin (Gibco). .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines HEK293 ATCC CRL-1573 HEK293T ATCC CRL-3216 HepG2 ATCC HB-8065 Hepa1-6 ATCC CRL-1830 Experimental models: Organisms/strains Zebrafish: AB strain China Zebrafish Resource Center N/A Mice: C57BL/6NCya-Ptprfem1/Cya cyagen Cat# S-KO-03926 Oligonucleotides Primers, see Table S1 This paper N/A siRNA targeting sequence: PTPRF #1: CCCUUUAAG AUUCUGUACAUU This paper N/A Software and algorithms GraphPad Prism 8.0 Graphpad Software https://www.graphpad.com/ Gator One software Gator Bio https://www.gatorbio.com/software/ 14 Cell Reports 45, 116974, February 24, 2026 .. In zebrafish studies, the C-terminal peptide of zebrafish Fbn1 and the extracellular ligand-binding domains (LBD) of Ptprfa and Ptprfb were expressed through GenScript (China).
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Stable Transfection:Article Title: Toxoplasma gondii GRA12 Inhibits the NF-ΚB Signaling Pathway by Targeting P65 and the IKK Complex
Article Snippet: .. HEK293T cells (obtained from the American Type Culture Collection, ATCC® CRL3216TM, Manassas, VA, USA), TLR4-293 cells (HEK293 cells stably transfected with human TLR4a, MD2, and CD14 genes; obtained from InvivoGen, #293-htlr4md2cd14, Hong Kong, China), and HFF cells (human foreskin fibroblasts; obtained from the American Type Culture Collection, ATCC® SCRC-1041TM) were maintained in DMEM (Thermo Fisher, Shanghai, China) supplemented with 10% fetal bovine serum (FBS; Thermo Fisher), 100 U/mL penicillin, and 100 μg/mL streptomycin in a humidified 5% CO2 atmosphere at 37 ◦C. .. HEK293T cells (obtained from the American Type Culture Collection, ATCC CRL3216, Manassas, VA, USA), TLR4-293 cells (HEK293 cells stably transfected with human TLR4a, MD2, and CD14 genes; obtained from InvivoGen, #293-htlr4md2cd14, Hong Kong, China), and HFF cells (human foreskin fibroblasts; obtained from the American Type Culture Collection, ATCC SCRC-1041) were maintained in DMEM (Thermo Fisher, Shanghai, China) supplemented with 10% fetal bovine serum (FBS; Thermo Fisher), 100 U/mL penicillin, and 100 μg/mL streptomycin in a humidified 5% CO2 atmosphere at 37 ◦C.
Transfection:Article Title: Toxoplasma gondii GRA12 Inhibits the NF-ΚB Signaling Pathway by Targeting P65 and the IKK Complex
Article Snippet: .. HEK293T cells (obtained from the American Type Culture Collection, ATCC® CRL3216TM, Manassas, VA, USA), TLR4-293 cells (HEK293 cells stably transfected with human TLR4a, MD2, and CD14 genes; obtained from InvivoGen, #293-htlr4md2cd14, Hong Kong, China), and HFF cells (human foreskin fibroblasts; obtained from the American Type Culture Collection, ATCC® SCRC-1041TM) were maintained in DMEM (Thermo Fisher, Shanghai, China) supplemented with 10% fetal bovine serum (FBS; Thermo Fisher), 100 U/mL penicillin, and 100 μg/mL streptomycin in a humidified 5% CO2 atmosphere at 37 ◦C. .. HEK293T cells (obtained from the American Type Culture Collection, ATCC CRL3216, Manassas, VA, USA), TLR4-293 cells (HEK293 cells stably transfected with human TLR4a, MD2, and CD14 genes; obtained from InvivoGen, #293-htlr4md2cd14, Hong Kong, China), and HFF cells (human foreskin fibroblasts; obtained from the American Type Culture Collection, ATCC SCRC-1041) were maintained in DMEM (Thermo Fisher, Shanghai, China) supplemented with 10% fetal bovine serum (FBS; Thermo Fisher), 100 U/mL penicillin, and 100 μg/mL streptomycin in a humidified 5% CO2 atmosphere at 37 ◦C.
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