rasamsonia byssochlamydoides nrrl 3658  (ATCC)

Journal: Cancers

Article Title: Transcriptomics and Metabolomics Integration Reveals Redox-Dependent Metabolic Rewiring in Breast Cancer Cells

doi: 10.3390/cancers13205058

Figure Lengend Snippet: Metabolic phenotype characterization of the MDA-MB231 triple-negative breast cancer cell line. ( A ) Proliferation curves of MCF102A ( – ) and MDA-MB231 ( – ) cells. Cells were grown in 6-well plates in the appropriate growth medium. Cells were collected and counted at the indicated time points. ( B , C ) Extracellular uptake of Glc/Gln and secretion of Lac/Glu in MCF102A ( – ) and MDA-MB231 ( – ) cells grown for 48 h determined enzymatically using YSI2950 bioanalyzer. ( D ) Mitochondrial respiration reflected by OCR levels in MCF102A ( – ) and MDA-MB231 ( – ) cells under basal conditions or following the addition of the indicated drugs ( n = 5). ( E ) NADH/NAD + ratio in MCF102A ( – ) and MDA-MB231 ( – ) cells obtained by colorimetric assay ( n = 9). ( F) Intracellular ROS levels in MCF102A ( – ) and MDA-MB231 ( – ) cells measured by DCFDA staining. ( G ) GSH/GSSG ratio in MCF102A ( – ) and MDA-MB231 ( – ) cells based on relative abundance obtained by LC-MS analysis. ( H ) Relative metabolites abundance of the glycolysis (left panel) and TCA cycle (right panel) pathways in MCF102A ( – ) and MDA-MB231 ( – ) cells. ( I ) Untargeted metabolic profiling of MDA-MB231 and MCF102A cell lines grown in standard growth conditions. Hierarchical clustering heatmaps, obtained by Metaboanalyst 5.0, show significantly different intracellular metabolites by LC-MS. ( J ) Representative images of the western blot analysis of MCF102A and MDA-MB231 reporting CtBP1, CtBP2, and β-actin expression (upper panels) (relative uncropped western blot and densitometry signals can be found in ). Relative densitometry analyses of CtBP1 and CtBP2 expression in the MDA-MB231 cell line compared to MCF102A (lower panels). Error bars indicate SD ( n = 3, unless otherwise noted). ** p -value < 0.005; * p -value < 0.01.

Article Snippet: MDA-MB231 and MCF102A cell lines were obtained from the American Type Culture Collection (ATCC) (LGC Standard, Teddington, UK).

Techniques: Colorimetric Assay, Staining, Liquid Chromatography with Mass Spectroscopy, Western Blot, Expressing

Journal: Cancers

Article Title: Transcriptomics and Metabolomics Integration Reveals Redox-Dependent Metabolic Rewiring in Breast Cancer Cells

doi: 10.3390/cancers13205058

Figure Lengend Snippet: Metabolic profiling of MCF102A and MDA-MB231 under drug treatment. ( A , B ) Untargeted metabolic profiling of control and HIPP-treated MCF102A (upper panel) and MDA-MB231 (lower panel) cell lines ( A ), or control and P4-treated MCF102A (upper panel) and MDA-MB231 (lower panel) cell lines ( B ) grown in standard growth conditions. Hierarchical clustering heatmaps, obtained by Metaboanalyst 5.0, show significantly different intracellular metabolites detected by LC-MS.

Article Snippet: MDA-MB231 and MCF102A cell lines were obtained from the American Type Culture Collection (ATCC) (LGC Standard, Teddington, UK).

Techniques: Control, Liquid Chromatography with Mass Spectroscopy

Journal: Cancers

Article Title: Transcriptomics and Metabolomics Integration Reveals Redox-Dependent Metabolic Rewiring in Breast Cancer Cells

doi: 10.3390/cancers13205058

Figure Lengend Snippet: Glucose metabolic rewiring by (U- 13 C 6 ) glucose labeling. ( A , B ) Atom transition map of (U- 13 C 6 ) glucose (blue circles) used to detect metabolic changes of control or drug-treated MDA-MB231 ( A ) or MCF102A ( B ). Filled circles indicate 13C enrichment. ** p -value < 0.005; * p -value < 0.01.

Article Snippet: MDA-MB231 and MCF102A cell lines were obtained from the American Type Culture Collection (ATCC) (LGC Standard, Teddington, UK).

Techniques: Labeling, Control