murine endothelial cells 3b11  (ATCC)

Journal: PLoS Biology

Article Title: Phosphorylation of seryl-tRNA synthetase by ATM/ATR is essential for hypoxia-induced angiogenesis

doi: 10.1371/journal.pbio.3000991

Figure Lengend Snippet: ( A ) Western blot to measure the expression of SerRS proteins in mouse 3B11 endothelial cells stably transfected with tag-free mouse SerRS WT or mutants (SerRS AA and SerRS DD ). The SerRS levels are quantified by the density of the bands and indicated relative to the level of the endogenous SerRS (Vector). ( B , C ) Matrigel plug angiogenesis assay performed with stably transfected 3B11 cells in C3H/HeJ mice. Matrigel plugs (dash lines enclosed regions) excised 14 days after implantation were analyzed by immunofluorescence for Vegfa ( B ) and quantified ( C ) (means ± SEM, n = 6 individual plugs per group, * p < 0.05, ** p < 0.01, *** p < 0.001, n.s., Student t test). See for quantitative data and statistical analysis. See for original, uncropped images supporting blots and gel results. n.s., not significant; SerRS, seryl-tRNA synthetase; SerRS AA , S101A/S241A; SerRS DD , S101D/S241D; SerRS WT , wild-type SerRS; Vegfa, vascular endothelial growth factor A

Article Snippet: Human HEK293 and MDA-MB-231 and mouse 3B11 cell lines were purchased from American Type Culture Collection (ATCC, Manassas, Virginia, United States of America) and cultured in Dulbecco's Modified Eagle Medium (ThermoFisher Scientific, Grand Island, New York, USA) supplemented with heat-inactivated fetal bovine serum (Omega Scientific, Tarzana, California, USA) to a final concentration of 10%.

Techniques: Western Blot, Expressing, Stable Transfection, Transfection, Plasmid Preparation, Angiogenesis Assay, Immunofluorescence

Journal: Cell

Article Title: Senescence-Induced Vascular Remodeling Creates Therapeutic Vulnerabilities in Pancreas Cancer

doi: 10.1016/j.cell.2020.03.008

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: 3B11 (murine) , ATCC , Cat#CRL-2160; RRID:CVCL_5487.

Techniques: Recombinant, In Vitro, In Vivo, Membrane, Plasmid Preparation, Viability Assay, Staining, Reverse Transcription, SYBR Green Assay, RNA Sequencing, Sequencing, shRNA, Software, Gentle, Real-time Polymerase Chain Reaction