Journal: Nature Communications
Article Title: Alveolar epithelial cells mitigate neutrophilic inflammation in lung injury through regulating mitochondrial fatty acid oxidation
doi: 10.1038/s41467-024-51683-1
Figure Lengend Snippet: a , b Flow cytometric analyses ( a ) evaluating phospholipid synthesis in Cpt1a − / − and control MLE12 cells. The geometric mean fluorescent intensity (MFI) of phospholipid labeling ( b ) represents phospholipid synthesis activity (n = 3 replicates per group; representative data of two independent experiments). c – e Immunoblots ( c ) confirming CPT1a overexpression in Cpt1a OE MLE12 cells, generated through lentiviral transduction (control MLE12 cells generated through lentiviral transduction of empty construct; representative data of two independent experiments). d Flow cytometric analyses evaluating phospholipid synthesis assay of Cpt1a OE and control MLE12 cells, and I phospholipid synthesis activity is compared (n = 3 replicates per group; representative data of two independent experiments). f , g Flow cytometric analyses ( f ) evaluating phospholipid synthesis in AT2 cells, and the phospholipid synthesis activity ( g ) is compared (n = 3 replicates per group; representative data of two independent experiments). h , i Intracellular contents of PC and phosphatidylglycerol (PG) species in AT2 cells, and data of the top 5 abundant h PC and i PG species are shown (n = 12 mice per group; data represent results of two independent experiments). j , k The contents of PC and PG species in bronchoalveolar lavage fluid (BALF) samples, and data of the top five abundant j PC and k PG species are demonstrated (control mice n = 10, Cpt1a iΔAT2 mice n = 9; data represent results of two independent experiments). b , e , g , and h – k Data are represented as mean ± SEM, and b , e , g – k two-sided p values are calculated by unpaired Student’s t-tests ( h – k , only p values less than 0.05 are shown).
Article Snippet: Both MLE12 (CRL-2100) and HEK293T (CRL-3216) cell lines were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).
Techniques: Control, Labeling, Activity Assay, Western Blot, Over Expression, Generated, Transduction, Construct
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