airway epithelial growth medium (PromoCell)

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PromoCell airway epithelial growth medium
Airway Epithelial Growth Medium, supplied by PromoCell, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/C-21040/Airway+Epithelial+Cell+Growth+Medium+2/pm33386991-70-28-32
Average 90 stars, based on 6 article reviews

airway epithelial growth medium - by Bioz Stars, 2026-10

90/100 stars

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Article Title: Cabozantinib, a Promising Therapeutic Strategy for Resistant Head and Neck Squamous Cell Carcinoma Patients
Article Snippet: 8 1.. University Côte d’Azur, Institute for Research on Cancer and Aging of Nice (IRCAN), 9 CNRS UMR 7284; INSERM U1081, Centre Antoine Lacassagne, 06189 Nice, France 10 2.. LIA ROPSE, Laboratoire International Associé Université Côte d’Azur Centre 11 Scientifique de Monaco 12 3.

Article Title: Temporal dynamics of ovine airway epithelial cell differentiation at an air-liquid interface
Article Snippet: The cells were again centrifuged and resuspended in airway epithelial growth medium (AEGM) (Promocell).

Cell Culture:

Article Title: TGF-β1 increases permeability of ciliated airway epithelia via redistribution of claudin 3 from tight junction into cell nuclei.
Article Snippet: P a p p [ c m / s ] P a p p [ c m / s ] log10 (C TGF- 1 g/ml) cultivation time [d] T G F - + A 8 3 -0 1 1 μ M C T R L N a F lu o C T R L 4 k D a C T R L 2 0 k D a C T R L N a F lu o C T R L 4 k D a C T R L 2 0 k D a C T R L B M P 2 A c ti v in A T G F + D M H -1 T G F + A 8 3 -0 1 T G F + S B 5 0 5 1 2 4 T G F + L D N 2 1 2 8 5 4 T G F + M L 3 4 7 Hannover, project no. 2699-2015) or obtained from Epithelix (Geneva, Switzerland). .. For proliferation, 106 Passage 1 cells were thawed and transferred into cell culture flask (75 cm2 surface area, Sarstedt AG & Co. KG, Nümbrecht, Germany) with 20 ml airway epithelial growth medium (Promocell, Heidelberg, Germany) supplemented with 5 μg/ml Plasmocin prophylactic, 100 μg/ml Primocin and 10 μg/ml Fungin (all from InvivoGen, Toulouse, France) and were cultivated at submerged conditions at 37 °C, 5% CO2 and 95% humidity until reaching 90% confluence. .. Cells were detached using DetachKit (Promocell, Heidelberg, Germany), and 3.5 * 104 cells suspended in 200 μl airway growth medium per filter were transferred to collagen-coated filters placed in 600 μl airway growth medium and cultivated at submerged conditions at 37 °C, 5% CO2 and 95% humidity.

Article Title: Antimalarial activity and toxicity of Garcinia mangostana Linn.
Article Snippet: .. Normal human epithelial cell (HRE) was purchased from Promocell Co. Ltd. (Heidelberg, Germany) and cultured in renal epithelial cell growth medium 2 supplemented with SupplementPack (Promocell Co. Ltd., Heidelberg, Germany). ..

Sterility:

Article Title: Development of primary human nasal epithelial cell cultures for the study of cystic fibrosis pathophysiology.
Article Snippet: DEVELOPMENT OF PRIMARY HUMAN NASAL EPITHELIAL CELL CULTURES 1 FOR THE STUDY OF CYSTIC FIBROSIS PATHOPHYSIOLOGY 2 3 F de Courcey1, A Zholos2, H Atherton-Watson, MTS Williams1, P Canning1, H 4 Danahay, JS Elborn1, M Ennis1* 5 6 7 1 Centre for Infection and Immunity, Queen’s University Belfast, Health Sciences 8 Building, Lisburn Road, Belfast BT9 7BL, UK 9 10 Centre for Vision and Vascular Science, Queen’s University Belfast, Institute of Clinical 11 Science, Block A, Belfast BT12 6BA , UK and the Department of Biophysics, 12 Educational and Scientific Centre “Institute of Biology”, Taras Shevchenko Kiev 13 National University, 2 Academician Glushkov Avenue, Kiev 03187, Ukraine 14 15 Novartis Institutes for Biomedical Research, Horsham RH12 5AB, UK 16 17 Running Head: Nasal epithelial cell culture in cystic fibrosis 18 19 4 Joint senior authors 20 * Corresponding author 21 22 Correspondence details for the author are: Prof. Madeleine Ennis, Centre for Infection 23 and Immunity, Queen’s University Belfast, Health Sciences Building, Lisburn Road, 24 Belfast BT9 7AE, UK.. 25 Tel: +4428 90 972672 Fax: +44 2890972671.. E-mail: m.ennis@qub.ac.uk 26 27 Articles in PresS.

Modification:

Article Title: The potential of Olea europaea extracts to prevent TGFβ1-induced epithelial to mesenchymal transition in human nasal respiratory epithelial cells
Article Snippet: Next, the epithelial layer was separated from the tissue and minced completely before being digested in 0.6% Collagenase Type I (Worthington, USA) for 60 min in a shaker incubator at 37 °C. .. After the tissue was fully digested, it was centrifuged for 5 min at 2370× g . Then, the supernatant was discarded and the pellet washed with DPBS and re-centrifuged for 5 min at 2370× g . The cell pellets were suspended in growth medium consisting of Airway Epithelial Growth Medium (AEGM) (PromoCell, USA), Defined Keratinocyte Serum-Free Medium (DKSFM) (GIBCO, USA) and Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12 (F12: DMEM) supplemented with 5% Fetal Bovine Serum (FBS) (BioWest, USA), in a 1:1:2 ratio and seeded into a 6-well plate (Thermo Fischer, USA). ..

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